Introduction: Throughout the history of humankind, herbs had been playing a major role in the treatments of human illness. As a result of thousands of research works, humankind›s identified excellent herbs to treat various illnesses such as malignancy, diabetes, and atherosclerosis. One of them is weak wheat named Triticum aestivum Linn. Wheat germinate and buds 7-14 days after germination are rich in proteins, vitamins, minerals and chlorophyll, which has similar structure to blood hemoglobin. Furthermore, hemoglobin is rich in iron and chlorophyll is rich in magnesium. Therefore, it is called “green blood”. Internationally, the wheatgrass is being clinically studied as it has antioxidant, antibacterial, and therapeutic effects for diabetes, respiratory tract illness, urinary tract disorders, atherosclerosis, and tumor.
Although Mongolians had been using the wheatgrass as a nutritional supply for cattle, during recent years, as it was identified that health benefits for humans, wheatgrass is being used for a human meal. We aimed to cultivate wheat seed and conduct the phytochemical study. Method: In order to achieve our goal, we planted wheatgrass, prepared extract and identified some of the chemical constituents. Moreover, we germinated wheat seed, dried, grinded, and extracted it with hexane, chloroform, methanol, and distilled water by using Soxhlet apparatus.
A preliminary screening was carried by the application of various testing methods of Molisch’ test, Fehling’s test, Biuret test, Xantho protein test, Buchard’s test, Mayer’s test, Wagner’s test, Ferric chloride test, Lead acetate test, Ninhydrin test, Keller-Killiani test, Foam test for determining the presence of alkaloids, terpenes, steroids, saponins, flavonoids, polysaccharides, and tannins, respectively. Moreover, we used spectrophotometry to determine the chlorophyll content Conclusion All the extracts derived from T. aestivum L showed the qualitative presence of major phytochemicals including alkaloids, steroids, and tannins, flavonoids, carbohydrates, saponins, triterpenoids. It was found that chlorophyll content 66%±0.2.