Objective To explore the feasibility of extracting and transplanting the live cells from burn scab tissue combined with artificial dermal scaffold coverage for the treatment of deep Ⅱ-degree burn wounds.Methods Four female Bama miniature pigs aged 7 to 8 months were successfully anesthetized and 3 square skin deep Ⅱ-degree burn wound models with a side length of 50 mm were established on each side of the spine(each wound is divided into four parts of a square with a side length of 25 mm).A total of 24 wounds were established.The ipsilateral wounds were divided into the dermis group and the combination group.After the live cells from the scabs were extracted and replanted on the wound surface of the combined group,an artificial dermal scaffold was covered.The wound surface of the dermis group was simply covered with an artificial dermal scaffold.On 7,14,21,28 d of the experiment,5 mm diameter tissues corresponding to the central part of each wound surface after anesthesia were successively and simultaneously cut by circular drilling.The HE staining was performed to observe the coverage rate of wound epidermis,the Masson staining was used to observe the tissue collagen content,Ki-67 antigen was used to observe the proliferation rate of wound cells,and CD31 count was used to observe the neovascularization of wound capillaries.Results On 7 d of the experiment,no wound epidermis coverage was observed in both groups.The wound coverage rate on 14,21,28 d in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05),moreover,the wound coverage rate in the combination group was increased with the experimental time was extended(P＜0.05).The collagen content on 7 d of experiment in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05).The Ki-67 expression level of the wound tissue on 7-28 d in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05).There was no statistically significant difference in the CD31 expression level during 7-28 d of experiment between the two groups(P＞0.05).Conclusion The extraction and transplantation of live cells from deep burn scab tissue combined with artifi-cial dermal scaffolds coverage could partially repair burn deep Ⅱ-degree wound.

ObjectiveTo evaluate the application effect of homemade visible stomach tube guiding device in patients with gastric canal implantation under general anesthesia.MethodsFrom January to December 2015,90 patients who would undergo gastrointestinal or hepatobiliary surgery under general anesthesia with indwelling gastric tube were selected as the research object by purpose sampling method. 90 cases were divided into group A (conventional catheter),group B (guidewire guided) and group C (homemade visible stomach tube guiding device guided),with 30 cases in each group. All patients received gastric canal implantation immediately after endotracheal intubation under general anesthesia. We recorded the time of one-time successful gastric tube implantation,the times of implantation,the number of successful cases that implanted at a time,the total number of cases with successful implantation and the number of cases with throat mucosal injury.ResultsThe intubation time of group A and group B were longer than that of group C (P<0.05). The time in group A was the longest. Only 10 cases in group A were successful implanted in one time,19 cases in group B were successfully implanted in one time,and all the cases in group C were successfully implanted in one time. The one-time success rate of gastric canal implantation of group C (100.0%) was higher than that of group A (33.3%) and group B (63.3%) (P<0.05). The total success rate of gastric canal implantation of group A (53.3%) was lower than that of group B (90.0%) and group C (100%) (P<0.05). The incidence of throat mucosal injury of group C (3.3%) was lower than that of group A (33.3%) and group B (26.7%) (P<0.05).ConclusionsThe homemade visible stomach tube guiding device can quickly improve the one-time success rate of gastric canal implantation in patients under general anesthesia,reduce the time of implantation and decrease the incidence of throat mucosal injury.

Objective To investigate anesthesia efficacy and safety between two drugs joint propofol intrave-nous anesthesia for colonoscopy,to provide reference for clinical treatment.Methods 120 routine colonoscopy patients were selected,they were randomly divided into control group and observation group,60 cases in each group. Patients of the control group were given ketamine and propofol before examination.Patients in the observation group were given fentanyl and propofol for intravenous anesthesia before the examination.The arterial pressure MAP,heart rate,HR,pulse oximetry SpO2 ,recovery time,time away from the hospital,anesthetic effects and adverse reactions were compared between the two groups.Results Anesthesia excellent rate in the observation group was 86.67%,that was 85.00% in the control group,the difference was not significant (χ2 =0.984,P =0.114).In the observation group,the recovery time and time away from the hospital were (7.44 ±1.24)min,(19.11 ±2.67)min,which were significantly shorter than those in the control group,the differences were statistically significant (t =5.847,P =0.004;t =6.922,P =0.001).The MAP,SpO2,HR at different time points showed statistically significant differences between the two groups(P >0.05).The incidence rate of adverse reactions in the observation group was 21.67%, that in the control group was 36.67%,the difference was not statistically significant (χ2 =5.665,P =0.007). Conclusion Fentanyl and propofol for intravenous anesthesia in colonoscopy has good anesthetic effect and good safety,which is worthy of clinical application.