Objective To investigate the relationships of expression of membrane palmitoylated protein 7(MPP7)in gastric cancer(GC)tissues with clinicopathological features and prognosis.Methods A total of 213 GC patients in the hospital from March 2018 to April 2020 were selected as research objects,and their tumor tissues and adjacent non-tumor tissues were collected.Immunohisto-chemistry(IHC)was used to detect the protein expression level of MPP7 in the tumor tissues and ad-jacent non-tumor tissues of GC patients,and the relationship between MPP7 and clinicopathological features was analyzed.The Kaplan-Meier survival curve was used to analyze the relationship between MPP7 expression and prognosis of GC patients.Univariate and multivariate Cox regression analyses were used to explore the relevant factors influencing the prognosis of GC patients.Results Compared with adjacent non-tumor tissues,MPP7 protein was significantly highly expressed in GC tissues(P＜0.05).Among GC patients,those with high MPP7 expression had significantly higher ratios of lymph node metastasis,Ⅲ to Ⅳ stages of cTNM staging,poor differentiation,and muscular layer invasion compared with those with low MPP7 expression(P＜0.001).Among the 156 patients with high MPP7 expression,the survival rate was 8.33％(13/156);among 57 patients with low MPP7 expression,the survival rate was 52.63％(30/57).The results of the Kaplan-Meier survival curve showed a significant difference in survival rates between the high MPP7 expression patients and the low MPP7 expression patients(Log-rank x2=54.333,P＜0.001).Compared with GC patients in the survival group,those in the death group had significantly higher ratios of lymph node metastasis,Ⅲ to Ⅳ stages of cTNM staging,poor differentiation,muscular layer invasion,and high MPP7 expression(P＜0.001).The results of multivariate Cox regression analysis showed that lymph node metastasis,Ⅲ to Ⅳ stages of cTNM staging,poor differentiation,muscular layer invasion,and high MPP7 expres-sion were all risk factors influencing the prognosis of GC patients(P＜0.05).Conclusion High MPP7 expression is closely related to the clinicopathological features and prognosis of GC patients,and it may serve as a potential biomarker for predicting GC progression and poor prognosis.

Objective To solve the problems of clinical laboratory module in mobile medical unit such as miscellaneous and mixed equipment,disordered packages,and weak mobility and responsiveness.Methods The concept of modularization was used for the secondary optimization of clinical laboratory equipment and consumables.The specialized vehicle for clinical laboratory was designed with considerations on the advantages of clinical laboratory shelter in equipment and medical vehicle in mobility,so as the values of clinical laboratory module were enhanced in mobile medical unit.Results Modular and standardized management of clinical laboratory equipment was realized to solve the existing problems of equipment.Conclusion The equipment supportability and mobility are enhanced in mobile medical unit,so that the requirements can be fulfilled for medical support missions at peace time and wartime.

To explore rapid 3D reconstruction based on wiggle stereoscopy for cerebrovascular CT images to e-liminate the complicacy and inconvenience of the traditional methods. Focal parts of cerebrovascular CT images were segmented based on intelligent neural network fusion model. A 3D human body model base was established, and the focal parts were replaced with the corresponding ones in the base to realize cerebrovascular CT images 3D reconstruction. The 3D human body model base enhanced the efficiency of the cerebrovascular CT images 3D reconstruction greatly. The rapid reconstruction based on wiggle stereoscopy facilitates the display of cerebrovascular CT im-ages.

Objective To investigate the effect of FK506 on the in vitro ostengenic potential of rat bone marrow-derived mesenchymal stem cells(MSCs)and the dose-response effect of FK506(5-5000 nmol/ L)on the ostengenic potential of MSCs in vitro.Methods MSCs derived from primary culture were sub- cultured for 16 days under four conditions:(1)?-MEM containing L-ascorbicacid-2-phosphate(AsAP)and?-glycemphosphate(?-GP)(basic culture medium)as a control;(2) AsAP and?-GP(basic culture medi- um) plus dexamethasone;(3)AsAP and?-GP(basic culture medium)plus FK506,(4)AsAP and?-GP (basic culture medium)plus FK506 and dexamethasone.Osteogenie potential was determined by testing os- teoblastic morphology,cell proliferation,alkaline phosphatase(APase)activity,bone nodule formation and the expression of osteocalcin mRNA.Results FK506 promoted the proliferation of MSCs,induced mineral- izing bone-like nodule formation,and increased APase activity and the expression of osteocalcin mRNA.The data also showed that the efficacy of FK506 was greater when used in combination with dexamethasone.Opti- mal ostengenesis was achieved with?-MEM containing 0.25mmol/L AsAP,10mmol/L?-GP,10nmol/L dexa- methasone and 50nmol/L FK506.Conclusion FK506 has powerful ostengenic ablility.It can be consid- ered as an osteogenic agent to repair bone defects.