Objective To explore the signal transduction pathway of cyst fluid of Echinococcus granalosus in anti-parasite mechanisms through investigating the effect of cyst fluid on the expression of Toll-like receptor(TLR) in cells. Methods Changes of TLR4 and TGF-β1 expression of 7404 liver cells were detected by quantitative PCR. Results After treatment with increasing concentration cyst fluid the expres-sion of TLR4 was reduced. TGF-β1 expression of liver cells increased with the dose. TLR2 expressions in each group were very low. Conclusion Cyst fluid can increase the expression of TLR4, suggesting that the TLR4 signal transduction pathway involve anti-cyst fluid of Echinococcus granulosus. High concentrations of cyst fluid contribute to TGF-β1 expression which plays a role in immune evasion.

Objective To observe ultramicro pathologic change of rabbit brain central damaged tissue and peripheral tissue after LOT, to evaluate the changed structure of blood brain barrier (BBB) of peripheral tissue in acute stage. Method Seventy Newzealand rabbits were randomly screened from Zhejiang University Animal Experiment Center. By stereotaxic technique, semiconductor surgica laser fibers were inserted into right frontal lobes and heat treated to randomly build LITT Group A (2 W, 600 s, n = 20) and LITT Group B (15 W, 100 s, n = 20) brain damaged models successfully. Other 15 nomal rabbits were randomly distributed as mannitol perfusion group and fake operation group. The ultramicro structures in central thermodamaged tissue were observed with transmission electro microscope after LITT 3 h,6 h,12 h,24 h. In peripheral tissue, ultramicro morphologic changes of brain vessels and BBB were evaluated. S100B protein in serum and BBB indexe were measured at different stages post LITT. Experimental data were treated as one-factor analysis of variance and q test. Results The brain damage center connected the tip of laser fiber and turn into thermodamage tissue. The main structure changes were cytoclasis, damnification of cell membrum, swelling of cell organelle such as mitochondrion, endoplasmic reticulurn,disappearance of mitochondrion and sparseness of cytoplasm in local tissure. Heat energy conducted to damage peripheral tissue, some cells occured apoptosis in different stage. In acute stage after LITT, contracted capillary vessel, oncreted red cell, swell endothelium cell, broken base membrum, wide around clearance and destroyed aperture structure were identified. The levels of serum S100B and BBB indexe dramatically rised. The opening time of BBB in peripheral tissue was longer than mannital perfusion group. However at 24 h post LITT, they began to recover in Group A. The difference of serum S100B and BBB indexe between Group A and Group B has statistical significance ( P =0.0087). Conclusions With semiconductor laser heat treatment and stereotaxic techniqe, definite cells cytoclasis, cell membrance structures and chondriosome damage could be performed obviously in rabbit brain thermotherapy point. Apoptosis could be found in peripheral tissue, BBB could be opened in an acute stage. The opening time course of BBB was shortened in those LITT cases with small power. It shew us a new method to perform a safe and exact damage zone of brain for functional neurosurgery.

Objective To investigate the incidence of peptic ulcer (PU) in seafarers in recent 10 years.Methods Nine hundred and sixty-nine cases of gastroscopy data obtained through outpatient,inpatient and physical examinations from June 1998 to June 2008 were investigated and results were compared with those of the counterparts working on the land at the same seaport (the control group).Results In the seafarer group,416 cases of PU were screened,of which 203 were cases of duodenal ulcer (DU),197 were cases of gastric ulcer (GU) and 16 were cases of complex ulcer (CU).On the other hand,in the control group,179 eases of PU were screened,of which 90 were cases of DU,70 were cases of GU and 19 were cases of CU.For both groups,differences could be seen in the age of onset,site of ulcer and the state of helicobaeter pylori (HP).Incidence of peptic ulcer for the seafarer group was obviously higher than that of the control group.The age of initial onset for the seafarers was markedly smaller than that of the control ( P < 0.05).Conclusions Due to many influential factors,incidence of peptic ulcer was obviously higher among seafarers.Protective measures should be taken,so as to decrease its incidence.

Objective To investigate the incidence of peptic ulcer (PU) in seafarers in recent 10 years.Methods Nine hundred and sixty-nine cases of gastroscopy data obtained through outpatient,inpatient and physical examinations from June 1998 to June 2008 were investigated and results were compared with those of the counterparts working on the land at the same seaport (the control group).Results In the seafarer group,416 cases of PU were screened,of which 203 were cases of duodenal ulcer (DU),197 were cases of gastric ulcer (GU) and 16 were cases of complex ulcer (CU).On the other hand,in the control group,179 eases of PU were screened,of which 90 were cases of DU,70 were cases of GU and 19 were cases of CU.For both groups,differences could be seen in the age of onset,site of ulcer and the state of helicobaeter pylori (HP).Incidence of peptic ulcer for the seafarer group was obviously higher than that of the control group.The age of initial onset for the seafarers was markedly smaller than that of the control ( P < 0.05).Conclusions Due to many influential factors,incidence of peptic ulcer was obviously higher among seafarers.Protective measures should be taken,so as to decrease its incidence.

Objective To explore the relationship between blood lead and general symptoms in pediatric clinic.Methods Levels of the blood lead of 487 cases with symptoms and 80 normal children as control group were determined by atom absorbent spectrophotometry.Results The blood lead level in patient group was higher than that in control group(P