Objective:To explore the relationship between white matter integrity and cognitive function in first-episode children and adolescents with schizophrenia by diffusion tensor imaging (DTI).Methods:Totally 84 children and adolescents with first-episode schizophrenia who were hospitalized in the Department of Child and Adolescent Psychiatry at the Second Affiliated Hospital of Xinxiang Medical University from September 2017 to June 2021 were selected as the study group, and 35 healthy children and adolescents matched with the study group were selected as the control group. Positive and negative symptom scale (PANSS) was used to assess the patient's mental symptoms. Diffusion tensor imaging (DTI) scans were performed, and the structural integrity of white matter fibers in the brain was measured by tract-based spatial statistics (TBSS), with the indicators including fractional anisotropy (FA). The Chinese version of measurement and treatment research to improve cognition in schizophrenia consensus cognitive battery (MCCB) and the Stroop color-word test were used to evaluate the cognitive function of all subjects. SPSS 26.0 software was used to analyse the data by analysis of variance, independent sample t-test, Mann Whitney U-test and Pearson correlation analysis. Results:(1)The FA values of the right superior longitudinal tract (MNI: x=49, y=-40, z=31) and the left corticospinal tract (MNI: x=-27, y=-22, z=-19) in the study group were higher than those in the control group(both P<0.05). The FA values of the bilateral cingulate gyrus (MNI: x=-7, y=25, z=14; x=20, y=-50, z=25) and the occipital part of the corpus callosum radiation line (MNI: x=-19, y=-50, z=21) in the study group were lower than those in the control group(both P<0.05).(2)The trail making test score of the study group was higher than that of the control group( t=4.344, P<0.01), and the scores of the symbol coding, Hopkins verbal learning test-revised( HVLT-R), brief visuospatial memory test-revised(BVMT-R), word fluency test, number span, maze, Stroop word, Stroop color and Stroop color words were lower than those in the control group( t=-13.041--3.204, all P<0.05). (3) The FA value of right cingulate gyrus (hippocampus) was negatively correlated with the number span ( r=-0.269, P=0.023).The FA value in occipital part of the corpus callosum radiation line was positively correlated with the symbol code ( r=0.237, P=0.047).The FA value of the left lower longitudinal bundle was negatively correlated with the Stroop color and maze ( r=-0.289, -0.275; P=0.010, 0.020).The FA value of the right inferior longitudinal fasciculus was negatively correlated with the score of maze ( r=-0.254, P=0.032). The FA value of occipital part of the corpus callosum radiation line was positively correlated with positive symptom score ( r=0.170, P=0.040). Conclusion:Cognitive dysfunction in children and adolescent with first-episode schizophrenia may be related to abnormal white matter integrity in the brain.

Objective To investigate the correlation between serum indirect bilirubin(IBIL)levels and carotid intima-media thickness(CIMT)of acute cerebral infarction patients.Methods A total of 229 patients with acute cerebral infarction at Wuxi No.2 Chinese Medicine Hospital from July 2021 to November 2023 were divided into the intima-media thickness group(CIMT≥0.9mm,n=108)and intima-media normal group(CIMT<0.9mm,n=121)according to CIMT values.The differences in gender,age,body mass index,smoking history,history of alcohol drinking,hypertension,coronary heart disease,diabetes,hyperlipidemia,total bilirubin(TBIL),direct bilirubin(DBIL),indirect bilirubin(IBIL),total cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,triglyceride,fasting blood glucose,uric acid,and cystatin C were compared between the two groups.To further elucidate the association between serum bilirubin levels and CIMT using both univariate and multivariate analysis.Results The two groups showed significant differences in gender,body mass index and IBIL in the results(P<0.05).However,there was no significant difference in age,history of alcohol drinking,smoking history,diabetes,coronary heart disease,hypertension,hyperlipidemia,TBIL,DBIL,total cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,triglyceride,fasting blood glucose,uric acid and cystatin C levels(P>0.05).Binary Logistic regression analysis indicated that gender,body mass index and IBIL were significant independent predictors of CIMT values(P<0.05).Conclusion Serum indirect bilirubin level is inversely associated with CIMT in acute cerebral infarction patients,which may be one of the protective factors of atherosclerosis.

Objective:To investigate the effects of oleuropein (OLE) on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway.Methods:SiHa cells were treated with 0-80 μ mol/L OLEs to detect cell survival rate and screen for the optimal drug concentration. SiHa cells were randomly separated into SiHa group, OLE low concentration (OLE-L) group, OLE medium concentration (OLE-M) group, OLE high concentration (OLE-H) group, and OLE-H+PI3K activator 740Y-P (OLE-H+740Y-P) group. CCK-8 method was applied to detect the proliferation activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells in each group. Flow cytometry was applied to detect the apoptosis rate of cells in each group. Western blot was used to detect the expression levels of PI3K-AKT signaling pathway related proteins, epithelial mesenchymal transition (EMT), and resistance related proteins such as epithelial cadherin, vimentin, N-cadherin, and P-glycoprotein (P-gp) of cells in each group.Results:OLEs with concentrations of 5, 10, and 20 μmol/L were selected for subsequent experiments. Compared with the SiHa group, the survival rate, numbers of clone formation, migration and invasion of cells, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, Vimentin, N-cadherin and P-gp in the OLE-L, OLE-M, and OLE-H groups gradually decreased ( P<0.05), while the apoptosis rate and E-cadherin expression level increased ( P<0.05). Compared to that before cisplatin treatment, the apoptosis rate of SiHa cells increased after cisplatin treatment ( P<0.05). The addition of PI3K activator 740Y-P on the basis of high concentration OLE reversed the trend of changes in the above indicators ( P<0.05) . Conclusions:OLE can inhibit the migration and invasion of cervical cancer cells, and improve their chemotherapy sensitivity. Its mechanism of action may be related to the inhibition of the PI3K-AKT signaling pathway.

Objective:To investigate the effects of oleuropein (OLE) on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway.Methods:SiHa cells were treated with 0-80 μ mol/L OLEs to detect cell survival rate and screen for the optimal drug concentration. SiHa cells were randomly separated into SiHa group, OLE low concentration (OLE-L) group, OLE medium concentration (OLE-M) group, OLE high concentration (OLE-H) group, and OLE-H+PI3K activator 740Y-P (OLE-H+740Y-P) group. CCK-8 method was applied to detect the proliferation activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells in each group. Flow cytometry was applied to detect the apoptosis rate of cells in each group. Western blot was used to detect the expression levels of PI3K-AKT signaling pathway related proteins, epithelial mesenchymal transition (EMT), and resistance related proteins such as epithelial cadherin, vimentin, N-cadherin, and P-glycoprotein (P-gp) of cells in each group.Results:OLEs with concentrations of 5, 10, and 20 μmol/L were selected for subsequent experiments. Compared with the SiHa group, the survival rate, numbers of clone formation, migration and invasion of cells, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, Vimentin, N-cadherin and P-gp in the OLE-L, OLE-M, and OLE-H groups gradually decreased ( P<0.05), while the apoptosis rate and E-cadherin expression level increased ( P<0.05). Compared to that before cisplatin treatment, the apoptosis rate of SiHa cells increased after cisplatin treatment ( P<0.05). The addition of PI3K activator 740Y-P on the basis of high concentration OLE reversed the trend of changes in the above indicators ( P<0.05) . Conclusions:OLE can inhibit the migration and invasion of cervical cancer cells, and improve their chemotherapy sensitivity. Its mechanism of action may be related to the inhibition of the PI3K-AKT signaling pathway.

Objective To analyze the eye lens equivalent dose levels of doctors during interventional cardiology procedures and identify related influential factors. Methods Twenty interventional specialists were selected from a cardiovascular specialty hospital. The cumulative equivalent doses to their eye lens during operations were monitored, and equipment-related parameters (fluoroscopy time, dose area product value [DAP], and entrance skin dose[ESD]), operation types, and operators’ positions were recorded. Results The annual equivalent doses to the eye lens of seven doctors exceeded 20 mSv. There was a linear correlation between the weekly number of operations and the equivalent dose to the eye lens (R² = 0.457, P = 0.001). The mean eye lens equivalent dose per operation was 17.1 μSv, showing linear correlations with fluoroscopy time, DAP values, and ESD values (R² = 0.427, 0.206, and 0.237, respectively, P < 0.05). The fluoroscopy time, DAP value, ESD value, and eye lens equivalent dose during percutaneous coronary intervention (PCI) were significantly higher than those during coronary angiography (t = −3.226, −3.108, −3.061, and −2.667, respectively, P < 0.03). Conclusion The annual equivalent doses to the eye lens are relatively high in interventional radiologists, some of whom may have values higher than the latest dose limit (20 mSv) suggested by the International Commission on Radiological Protection. Attention should be paid to operators performing PCI, and the workload optimization is necessary in practical operations to avoid unnecessary fluoroscopy time and reduce the eye lens doses of the operators.

Objective:This study aims to analyze the structural characteristics and fibrous structural integrity of the white matter of the brain and their relationship with obsessive-compulsive symptoms in children and adolescents with first-onset obsessive-compulsive disorder (OCD).Methods:Thirty-eight patients with OCD (OCD group) and 35 age-, sex-, and education-matched controls (control group) were included. The brain white matter volume was measured using voxel-based morphometry (VBM) with diffusion tensor imaging (DTI) scans. The tract-based spatial statistics (TBSS), including fractional anisotropy (FA), mean diffusivity (MD), and axial diffusivity (AD), were applied to measure the structural integrity of white matter fibres. The independent samples t-test was used to compare FA, MD, and AD values in differential brain areas between the 2 groups. The clinical characteristics of the patients were assessed using the Yale-Brown Obsessive-Compulsive Symptom Scale for Children. Pearson′s correlation analysis was used to analyze the relationship between the severity of the obsessive-compulsive symptoms and the white matter volume of the brain and the FA, MD, and AD values of the significantly different brain areas in the OCD group. Results:Compared to the control group, the OCD group had lower white matter volumes in the left inferior frontal gyrus, right inferior temporal gyrus, right inferior frontal gyrus, left parietal gyrus, and left limbic lobe ( t=-3.67, -4.21, -3.58, -3.70, -4.34, all P<0.01); had higher FA, MD, and AD values in the left corticospinal tract, left superior longitudinal tract; had higher MD and AD values in the right corticospinal tract, right and left anterior thalamic radiations, right and left inferior fronto-occipital gyrus, and left inferior longitudinal gyrus; had higher FA, MD, and AD values in the occipital and frontal part of the corpus callosum radiation; had a lower FA value in the left and right anterior thalamic radiation, left and right inferior frontal gyrus, left cingulate gyrus, left and right inferior longitudinal gyrus, and right superior longitudinal gyrus (temporal); and had a lower AD value in the left hook gyrus and right superior longitudinal gyrus. In the OCD group, the Pearson′s correlation analysis showed that the white matter volume of the right inferior frontal gyrus was negatively correlated with the obsessive-compulsive thinking factor score ( r=-0.355, P<0.05), and the left parietal gyrus was negatively correlated with the total obsessive-compulsive score, the obsessive-compulsive thinking factor score, and the obsessive-compulsive behavior factor score ( r=-0.361, -0.350, -0.376, all P<0.05); that the FA value in the occipital part of the corpus callosum radiation was negatively correlated with the total obsessive-compulsive score and the obsessive-compulsive behavior factor score ( r=-0.410, -0.411, both P<0.05), the MD values in the left and right anterior thalamic radiation were both negatively correlated with total compulsive scores ( r=-0.389, P<0.05; -0.489, P<0.01), the AD values in the left and right anterior thalamic radiation were both negatively correlated with total compulsive scores ( r=-0.446, -0.405, both P<0.05),the MD and AD values in the left and right anterior thalamic radiation were both negatively correlated with compulsive behavior factor scores ( r=-0.558, -0.631, -0.530, -0.539, all P<0.01), and the AD value in the left subfrontal occipital tract was negatively correlated with compulsive total score and compulsive thinking factor score ( r=-0.359, P<0.05; r=-0.499, P<0.01). Conclusion:Children and adolescents with OCD have reduced white matter volume and impaired structural integrity of white matter fibres, involving the cortico-striato-thalamo-cortical circuit and several brain regions, such as the parietal lobe, limbic lobe, corpus callosum, corticospinal tract, and inferior longitudinal tract. The abnormalities in white matter structure and structural integrity of fibres are correlated with the severity of OCD symptoms.

Objective:This study aims to analyze the structural characteristics and fibrous structural integrity of the white matter of the brain and their relationship with obsessive-compulsive symptoms in children and adolescents with first-onset obsessive-compulsive disorder (OCD).Methods:Thirty-eight patients with OCD (OCD group) and 35 age-, sex-, and education-matched controls (control group) were included. The brain white matter volume was measured using voxel-based morphometry (VBM) with diffusion tensor imaging (DTI) scans. The tract-based spatial statistics (TBSS), including fractional anisotropy (FA), mean diffusivity (MD), and axial diffusivity (AD), were applied to measure the structural integrity of white matter fibres. The independent samples t-test was used to compare FA, MD, and AD values in differential brain areas between the 2 groups. The clinical characteristics of the patients were assessed using the Yale-Brown Obsessive-Compulsive Symptom Scale for Children. Pearson′s correlation analysis was used to analyze the relationship between the severity of the obsessive-compulsive symptoms and the white matter volume of the brain and the FA, MD, and AD values of the significantly different brain areas in the OCD group. Results:Compared to the control group, the OCD group had lower white matter volumes in the left inferior frontal gyrus, right inferior temporal gyrus, right inferior frontal gyrus, left parietal gyrus, and left limbic lobe ( t=-3.67, -4.21, -3.58, -3.70, -4.34, all P<0.01); had higher FA, MD, and AD values in the left corticospinal tract, left superior longitudinal tract; had higher MD and AD values in the right corticospinal tract, right and left anterior thalamic radiations, right and left inferior fronto-occipital gyrus, and left inferior longitudinal gyrus; had higher FA, MD, and AD values in the occipital and frontal part of the corpus callosum radiation; had a lower FA value in the left and right anterior thalamic radiation, left and right inferior frontal gyrus, left cingulate gyrus, left and right inferior longitudinal gyrus, and right superior longitudinal gyrus (temporal); and had a lower AD value in the left hook gyrus and right superior longitudinal gyrus. In the OCD group, the Pearson′s correlation analysis showed that the white matter volume of the right inferior frontal gyrus was negatively correlated with the obsessive-compulsive thinking factor score ( r=-0.355, P<0.05), and the left parietal gyrus was negatively correlated with the total obsessive-compulsive score, the obsessive-compulsive thinking factor score, and the obsessive-compulsive behavior factor score ( r=-0.361, -0.350, -0.376, all P<0.05); that the FA value in the occipital part of the corpus callosum radiation was negatively correlated with the total obsessive-compulsive score and the obsessive-compulsive behavior factor score ( r=-0.410, -0.411, both P<0.05), the MD values in the left and right anterior thalamic radiation were both negatively correlated with total compulsive scores ( r=-0.389, P<0.05; -0.489, P<0.01), the AD values in the left and right anterior thalamic radiation were both negatively correlated with total compulsive scores ( r=-0.446, -0.405, both P<0.05),the MD and AD values in the left and right anterior thalamic radiation were both negatively correlated with compulsive behavior factor scores ( r=-0.558, -0.631, -0.530, -0.539, all P<0.01), and the AD value in the left subfrontal occipital tract was negatively correlated with compulsive total score and compulsive thinking factor score ( r=-0.359, P<0.05; r=-0.499, P<0.01). Conclusion:Children and adolescents with OCD have reduced white matter volume and impaired structural integrity of white matter fibres, involving the cortico-striato-thalamo-cortical circuit and several brain regions, such as the parietal lobe, limbic lobe, corpus callosum, corticospinal tract, and inferior longitudinal tract. The abnormalities in white matter structure and structural integrity of fibres are correlated with the severity of OCD symptoms.

Objective:To summarize the anesthesia management of living small bowel transplantation.Methods:Severn patients undergoing living and allogeneic small bowel transplantation for the first time were selected.The intraoperative hemodynamics, indexes of blood gas analysis, body temperature and blood transfusion and volume of liquid infused were analyzed.Postoperative outcomes were tracked.Results:Six cases survived and were successfully discharged from hospital successfully, and one patient died.In the operation room, 71% patients were successfully extubated after surgery.Compared with the values during anatomical separation period, Hb during vascular anastomosis and intestinal reconstruction periods and concentration of Ca 2+ during intestinal reconstruction period were significantly decreased, and the blood glucose concentration during vascular anastomosis period were increased ( P<0.05 or 0.01). Compared with the values during vascular anastomosis period, the blood glucose concentration was increased significantly during intestinal reconstruction period ( P<0.05). Crystalloid solution (57±30) ml/kg and colloid solution which mainly containing 20% albumin (15±13) ml/kg were infused mainly during anatomical separation and vascular anastomosis periods in all the patients. Conclusion:The condition of successful living small bowel transplantation is fully evaluation and preparation before surgery.Intravenous-inhalational anesthesia combined with transverses abdominis plane block and rational infusion of colloid solution with vasoactive drugs to maintain hemodynamics stability and monitor blood gas, body temperature, active adjustment of electrolytes and internal environment and stable body temperature can be helpful in maintaining perioperative stable vital signs during the perioperative period, removing the tracheal tube early at the end of surgery, and reducing the development of postoperative complications in patients undergoing living small bowel transplantation.

Objective:To investigate the effect of low expression of human epidermal growth factor-like domain protein 7 (EGFL7) gene in cervical cancer cell Hela on its migration and invasion ability.Methods:Cells in the experimental group used small interfering RNA (siRNA) to target the human EGFL7 gene to reduce the expression of EGFL7 in human cervical cancer cells Hela, and cells in the control group were transfected with Mock-siRNA. Real-time quantitative PCR was used to detect the EGFL7 mRNA content of cancer cells in each group; Western blot was used to detect EGFL7 protein expression of cancer cells in each group; The cell scratch healing experiment and Transwell experiment were used to detect the migration and invasion ability of Hela cells in each group.Results:siRNA reduced the protein expression of EGFL7 in human cervical cancer cell Hela. The wound closure percentage of Hela cells in the control group was 74.1%±6.8%. After the expression of EGFL7 was reduced, the percentage of cervical cancer cells was 42%±4.9%, and the wound closure ability was significantly reduced ( P<0.05) . The results of Transwell cell transfer showed that the number of cells successfully transferred by Hela cells in the control group was 179.24±20.01, while the number of cells successfully transferred by Hela cells with low EGFL7 expression was 79.22±13.16. The transfer ability of cells transfected with EGFL7-siRNA was significantly reduced ( P<0.05) . The results of invasion experiments showed that the number of successfully transferred cells in the control group was 79.35±8.04, the number of cells successfully transferred in the EGFL7-siRNA group was 26.98±6.24, and the invasion ability of Hela cells with low expression of EGFL7 decreased ( P< 0.05) . The expression of E-cad in Hela cells with low expression of EGFL7 was up-regulated, and the expression of MMP2/9 protein was down-regulated (all P<0.05) . Conclusion:The low expression of EGFL7 can reduce the migration and invasion ability of cervical cancer cell Hela through the EMT pathway.

Objective:Establishment of a new model of human primary colon cancer transplantation tumor in normal immune mice and to provide a reliable experimental animal model for studying the pathogenesis of colon cancer under normal immunity.Methods:Human colon cancer cells come from colon cancer patients who underwent surgery in the Affiliated Hospital of Jining Medical College in 2017. The mice in the cell control group were inoculated with phosphate buffered solution (PBS) containing colon cancer cells, the microcarrier control group was inoculated with PBS containing microcarrier 6, and the cell-microcarrier complex group was inoculated with the PBS containing colon cancer cell-microcarrier complex. The cells of each group were inoculated under the skin of the right axilla of mice by subcutaneous injection, and the time, size, tumor formation rate and pathological changes under microscope were recorded. The transplanted tumor tissue was immunohistochemically stained with the EnVisiion two-step method, and the tumor formation rate of the transplanted tumor was judged according to the proportion of positive cells in the visual field. The polymerase chain reaction (PCR) method was used to detect the expression of human-specific Alu sequence in mice tumor tissue.Results:After inoculation with tumor cells, the mice in the cell control group and the microcarrier control group did not die and did not form tumors; the mice in the cell-microcarrier complex group had palpable subcutaneous tumors in the right axillary subcutaneously on the 5th to 7th days after inoculation, and tumor formation rate is 67% (10/15), and the tumor volume can reach about 500 mm 3 2 to 3 weeks after vaccination. The immunohistochemistry results showed that CK20, CDX-2 and carcinoembryonic antigen were all positively expressed. The PCR results showed that the expression of human-specific Alu sequence can be detected in the transplanted tumor tissue of tumor-bearing mice. Conclusion:Human primary colon cancer cells used microcarrier 6 as a carrier to form tumors in normal immunized mice, and successfully established a new model of human colon cancer transplantation tumor in normal immune mice.