To establish murine graft versus host disease (GVHD) model, in order to evaluate the efficacy of human CD40 Ig fusion protein treatment. To establish murine GVHD model, 2 5?10 7 or 5 0?10 7 /L spleen cells of male C57BL/6 mice were intravenously injected into female BALB/c mice, as receipt, respectively, after sub lethally irradiation by 60 Co source. After the induction of GVHD, human CD40 Ig fusion protein was intravenously injected three times at a dose of 50?g, 150?g and 450?g on day 0, 2 and 4, respectively. The results showed that the typical expressions of GVHD were observed 4 or 5 days after the injection of donor spleen cells, and specific male Y chromosome fragment was amplified by genomic PCR in female BALC/c receipts. The mean survival time (MST) of GVHD induced mice was significantly prolonged by the treatment of human CD40 Ig fusion protein. It is suggested that Murine GVHD model successfully established can be used in evaluating the effects of anti GVHD therapies.

To investigate the acute and late side effects of autologous peripheral blood stem cell transplantation (Auto PBSCT) in children. Acute toxicity was evaluated in 21cases, according to Beurmen criteria. 11 patients who lived longer than 3 years after Auto PBSCT were followed up. Acute toxicity was mild. Growth and neuropsychological development were normal. Two children developed slight compensated hypothyroidism. Puberty was delayed in one male patient. Mild cataract was noticed in six children. Seven patients were found to suffer from hepatitis. The results suggested that pediatric patients were able to tolerate an appropriate pre conditioning regimen. Attention shoud be paid to the prevention of hepatitis.