Background: Gurgem-13, a traditional Mongolian medicinal formula, is described in the Four Medical Tantras as having therapeutic effects against liver disorders, toxins, renal “wind” conditions, urinary retention, hot-natured conditions, and parasitic infections. It has been widely used in Mongolian traditional medical practice for the treatment of various liver diseases. Aim: To evaluate the effects of Gurgem-13 fractions on the viability of LX-2 cells (human hepatic stellate cell line). Materials and Methods: Gurgem-13 powder was extracted by multiple solvents, including acetonitrile, methanol, distilled water, petroleum ether, n-hexane, ethyl acetate, and anhydrous ethanol, to obtain distinct fractions. LX-2 cells were cultured under standard conditions and treated with varying concentrations of each fraction. Cell proliferation was assessed using the MTT assay. Results: All Gurgem-13 fractions exhibited dose-dependent inhibitory effects on LX-2 cell proliferation. At concentrations of 50 and 100 µL/mL, a statistically significant reduction in cell proliferation was observed (p<0.05). Among the tested fractions, the ethyl acetate fraction demonstrated the highest inhibitory activity. Conclusion Gurgem-13 fractions, particularly the ethyl acetate fraction, significantly suppresses the proliferation of hepatic stellate cells in vitro, suggesting potential antifibrotic activity. These findings support the traditional use of Gurgem-13 in liver disease and suggest its potential therapeutic value against liver fibrosis.