Objective:To investigate the effect of long non-coding RNA (lncRNA) H19 regulating miRNA-675 (miR-675) and phosphatase and tensin homologue-deleted chromosome ten gene (PTEN) on the cell proliferation of glioma.Methods:Glioma cell lines U87-MG and U251 were chosen. The siRNA online design tool wad used to design small interfering RNA (siRNA) targeting H19. U87-MG and U251 cell lines with the stable knockdown of H19 were constructed (the stable knockdown of H19 group), and the cells randomly transfected with siRNA plasmid were taken as the control group, and normal cultured cells were treated as the blank group. Additionally, miR-675 and control microRNA were transfected into U87-MG and U251 with the stable knockdown of H19 (the overexpressing miR-675 group and the corresponding control group). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of miR-675 and H19 in each group; the methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation ability; the dual luciferase reporter gene assay was used to verify the targeting relationship between miR-675 and PTEN; Western blot was used to detect the relative expression level of PTEN protein.Results:The MTT assay results showed that the proliferation ability of U87-MG and U251 cells in the stable knockdown of H19 group was lower than that of the corresponding control group; and the differences in cell proliferation ability of all the groups after 48 h of culture were statistically significant (all P < 0.05). qRT-PCR detection results showed that the relative expression level of miR-675 in U251 cells in the stable knockdown of H19 group and the corresponding control group was 0.329±0.009 and 1.043±0.087, respectively, and the difference was statistically significant ( t = 14.15, P < 0.001); the relative expression level of miR-675 in U87-MG cells in the stable knockdown of H19 group and the corresponding control group was 0.299±0.009 and 1.027±0.106, respectively, and the difference was statistically significant ( t = 11.85, P < 0.001); the relative expression level of miR-675 in U87-MG and U251 cells in the stable knockdown of H19 group was lower than that of the corresponding control group. The dual luciferase reporter gene assay verified that miR-675 could bind to the 3'-UTR of PTEN. Western blot detection results showed that the relative expression level of PTEN protein in U87-MG and U251 cells in the stable knockdown of H19 group was higher than that of the corresponding control group and the blank group; in the U87-MG and U251 cells in the stable knockdown of H19 group, the relative expression level of PTEN in the overexpressing miR-675 group was lower than that of the corresponding blank group and the control group. In the U87-MG and U251 cells in the stable knockdown of H19 group, the cell proliferation ability of the overexpressing miR-675 group was higher than that of the corresponding blank group and the control group; the differences in cell proliferation ability of all the groups after 48 h of culture were statistically significant (all P < 0.05). Conclusions:lncRNA H19 may regulate the cell proliferation of glioma cells through the miR-675-PTEN signaling pathway.

Background In recent years, the rising number of e-cigarette users among adolescents and the surging cases of lung injury related to e-cigarette use have attracted the attention of researchers in various fields. Objective To identify the research hotspots and trends of e-cigarette or vaping product use associated lung injury (EVALI) worldwide from 2013 to 2022 by bibliometric and visual analysis. Methods Web of Science Core Collection was selected to obtain literature related to EVALI from 2013 to 2022 across the world, statistics were calculated by country/region, institution, author, journal, cited literature, keyword, etc. CiteSpace 6.2.R1 was used for visual analysis to draw diagrams of publication trend, author cooperation network, co-citation clustering time distribution, and keyword cluster. Results A total of 888 EVALI-related papers published between 2013 and 2022 were retrieved. The number of publications was gradually increased, with a significant increase in 2020 and a decrease from 2021, but the number of citations was increased year by year. The most active country was the United States (631 articles). European and American countries cooperated closely and the centrality was prominent. Among the publishing institutions, the University of California system topped the list with 103 articles. Rahman I (27 articles) published the most articles and had a high degree of centrality; Goniewicz M L was the most cited author; and the network analysis diagram showed relatively weak collaboration between authors. The American Journal of Respiratory and Critical Care Medicine was the journal with the highest number of publications (94 articles). The top 5 cited articles were all cited more than 300 times. The leading high-frequency keywords of EVALI-related studies were nicotine (149 times), exposure (118 times), and oxidative stress (80 times). The cluster of key nodes in the co-citation network and the clustering time distribution diagram indicated youth e-cigarette addiction received widespread attention from society. From the top 25 keywords with the strongest bursts, the focus of research on the pathogenesis of EVALI gradually shifted from the oxidative stress damage associated with e-cigarette vapor to the oxidative effect of flavoring chemicals in the process of lung injury. The current research interests in this field were mainly the mechanisms of various chemicals in e-cigarettes and the heating elements that led to damage to the lungs. Conclusion EVALI is receiving continuous attention from researchers in government, medical institutions, and other organizations. A variety of e-cigarette ingredients such as flavoring chemicals may lead to varying degrees of cytotoxicity, inflammation, and lung damage. However, the pathophysiology of EVALI remains unclear. In the future, more Chinese scholars should be encouraged to participate in this field.

Objective:To explore the effect and mechanism of naringenin (NAR) on glucose and lipid metabolism and oxidative stress in rats with gestational diabetes mellitus (GDM) .Methods:10 normal pregnant rats were selected as the control group, and the GDM model was prepared. One-time tail iv streptozotocin (STZ, 35 mg/kg) was used to construct GDM model. The 50 rats successfully modeled were divided into model group, metformin group (Met group, 20 mg/kg), low NAR (NAR-L, 50 mg/kg), high (NAR-H, 100 mg/kg) dose groups and NAR+EX527 group (NAR 100 mg/kg+EX527 10 mg/kg), 10 rats per group. Rats in Met group and NAR low-dose and high-dose groups were given corresponding doses of drugs ig. Rats in NAR+EX527 group were given NAR ig and EX527 ip at the same time, once a day, for 28 consecutive days. The levels of fasting blood glucose (FBG) and fasting serum insulin (FINS) in rats were detected, and the homeostasis model assessment for insulin resistance (HOMA-IR) was calculated; the levels of serum triglycerides (TG), cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) were detected; the level of malondialdehyde (MDA) in pancreatic tissue and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected; the tissue lesions of pancreas and placenta were observed with HE staining; the expression of pancreatic tissue AMP-activated protein kinase (AMPK), p-AMPK, silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1 α) proteins was detected with Western blotting method. Results:Compared with control, the FBG, FINS, HOMA-IR, serum TC, TG, LDL-C levels, and pancreatic tissue MDA level in the model group were significantly increased, the SOD and GSH-Px activities, pancreatic tissue p-AMPK/AMPK ratio and SIRT1 and PGC-1 α expression levels were significantly reduced ( P<0.05), and the placenta and pancreas tissues showed obvious pathological damage; Compared with the model group, the FBG, FINS, HOMA-IR, serum TC, TG, LDL-C levels, and pancreatic tissue MDA level in the Met group and the NAR-L and NAR-H groups were significantly reduced, the SOD and GSH-Px activities, pancreatic tissue p-AMPK/AMPK ratio and SIRT1 and PGC-1 α expression levels were significantly increased ( P<0.05), the placenta and pancreas tissues damage reduced; and on the basis of NAR intervention, the use of SIRT1 inhibitor EX527 could significantly reverse the protective effect of NAR on GDM rats. Conclusion:NAR may improve glucose and lipid metabolism disorder and oxidative stress response in GDM rats by activatin SIRT1/PGC-1 α pathway.

Objective:To analyze the influencing factors of incision infection in patients undergoing colorectal cancer surgery under the standards of enhanced recovery after surgery, to establish a prediction model for the risk of postoperative incision infection and to evaluate its efficacy, providing a reference for surgical site caring of colorectal cancer patients under enhanced recovery after surgery precisely.Methods:A cross-sectional survey was used, 285 patients who underwent colorectal tumor surgery in the Department of Gastrointestinal Surgery of the First Affiliated Hospital of Xinjiang Medical University and the First Department of Gastrointestinal Surgery and the Second Department of Gastrointestinal Surgery of Xinjiang Uygur Autonomous Region Cancer Hospital from July 2023 to January 2024 were prospectively selected as the study subjects by the convenience sampling method, 225 cases were used as the modeling group and 60 cases were selected as the external validation group. The preoperative data, intraoperative data and postoperative data of subjects were collected. Postoperative incision infection was taken as the outcome index. Univariate analysis and multivariate Logistic regression analysis were used to screen the independent risk factors of postoperative incision infection of patients with colorectal tumors, and drew a nomogram, Hosmer-Lemeshow test and receiver operating characteristic (ROC) curve were used to verify the model.Results:The modeling group consisted of 131 males and 94 females aged (58.42 ± 16.24) years old. There were 35 males and 25 females in the external validation group, with an age of (57.16 ± 16.28) years. Multivariate analysis showed that malnutrition ( OR=6.614, 95% CI 2.008-21.789), length of hospital stay ( OR=1.058, 95% CI 1.004-1.114), interleukin-6 ( OR=1.041, 95% CI 1.021-1.062), and lymphocyte count ( OR=0.275, 95% CI 0.093-0.813) were independent risk factors for incision infection (all P<0.05). The area under the ROC curve of the modeling group was 0.921, the 95% CI was 0.754-0.902, the optimal cut-off value was 0.461, the sensitivity was 0.782, and the specificity was 0.785, concordance index of calibration curve was 0.971. External validation results showed that area under the ROC curve was 0.828, the 95% CI was 0.754-0.902, the optimal cut-off value was 0.438, the sensitivity was 0.745 and the specificity was 0.783. Conclusions:Under the enhanced recovery after surgery, the risk prediction model established in this study has good effect in predicting postoperative incision infection, which can provide a reference for early identification of high-risk patients with postoperative incision infection after colorectal cancer surgery.

Objective:To explore the effect and mechanism of naringenin (NAR) on glucose and lipid metabolism and oxidative stress in rats with gestational diabetes mellitus (GDM) .Methods:10 normal pregnant rats were selected as the control group, and the GDM model was prepared. One-time tail iv streptozotocin (STZ, 35 mg/kg) was used to construct GDM model. The 50 rats successfully modeled were divided into model group, metformin group (Met group, 20 mg/kg), low NAR (NAR-L, 50 mg/kg), high (NAR-H, 100 mg/kg) dose groups and NAR+EX527 group (NAR 100 mg/kg+EX527 10 mg/kg), 10 rats per group. Rats in Met group and NAR low-dose and high-dose groups were given corresponding doses of drugs ig. Rats in NAR+EX527 group were given NAR ig and EX527 ip at the same time, once a day, for 28 consecutive days. The levels of fasting blood glucose (FBG) and fasting serum insulin (FINS) in rats were detected, and the homeostasis model assessment for insulin resistance (HOMA-IR) was calculated; the levels of serum triglycerides (TG), cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) were detected; the level of malondialdehyde (MDA) in pancreatic tissue and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected; the tissue lesions of pancreas and placenta were observed with HE staining; the expression of pancreatic tissue AMP-activated protein kinase (AMPK), p-AMPK, silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1 α) proteins was detected with Western blotting method. Results:Compared with control, the FBG, FINS, HOMA-IR, serum TC, TG, LDL-C levels, and pancreatic tissue MDA level in the model group were significantly increased, the SOD and GSH-Px activities, pancreatic tissue p-AMPK/AMPK ratio and SIRT1 and PGC-1 α expression levels were significantly reduced ( P<0.05), and the placenta and pancreas tissues showed obvious pathological damage; Compared with the model group, the FBG, FINS, HOMA-IR, serum TC, TG, LDL-C levels, and pancreatic tissue MDA level in the Met group and the NAR-L and NAR-H groups were significantly reduced, the SOD and GSH-Px activities, pancreatic tissue p-AMPK/AMPK ratio and SIRT1 and PGC-1 α expression levels were significantly increased ( P<0.05), the placenta and pancreas tissues damage reduced; and on the basis of NAR intervention, the use of SIRT1 inhibitor EX527 could significantly reverse the protective effect of NAR on GDM rats. Conclusion:NAR may improve glucose and lipid metabolism disorder and oxidative stress response in GDM rats by activatin SIRT1/PGC-1 α pathway.

Objective:To analyze the influencing factors of incision infection in patients undergoing colorectal cancer surgery under the standards of enhanced recovery after surgery, to establish a prediction model for the risk of postoperative incision infection and to evaluate its efficacy, providing a reference for surgical site caring of colorectal cancer patients under enhanced recovery after surgery precisely.Methods:A cross-sectional survey was used, 285 patients who underwent colorectal tumor surgery in the Department of Gastrointestinal Surgery of the First Affiliated Hospital of Xinjiang Medical University and the First Department of Gastrointestinal Surgery and the Second Department of Gastrointestinal Surgery of Xinjiang Uygur Autonomous Region Cancer Hospital from July 2023 to January 2024 were prospectively selected as the study subjects by the convenience sampling method, 225 cases were used as the modeling group and 60 cases were selected as the external validation group. The preoperative data, intraoperative data and postoperative data of subjects were collected. Postoperative incision infection was taken as the outcome index. Univariate analysis and multivariate Logistic regression analysis were used to screen the independent risk factors of postoperative incision infection of patients with colorectal tumors, and drew a nomogram, Hosmer-Lemeshow test and receiver operating characteristic (ROC) curve were used to verify the model.Results:The modeling group consisted of 131 males and 94 females aged (58.42 ± 16.24) years old. There were 35 males and 25 females in the external validation group, with an age of (57.16 ± 16.28) years. Multivariate analysis showed that malnutrition ( OR=6.614, 95% CI 2.008-21.789), length of hospital stay ( OR=1.058, 95% CI 1.004-1.114), interleukin-6 ( OR=1.041, 95% CI 1.021-1.062), and lymphocyte count ( OR=0.275, 95% CI 0.093-0.813) were independent risk factors for incision infection (all P<0.05). The area under the ROC curve of the modeling group was 0.921, the 95% CI was 0.754-0.902, the optimal cut-off value was 0.461, the sensitivity was 0.782, and the specificity was 0.785, concordance index of calibration curve was 0.971. External validation results showed that area under the ROC curve was 0.828, the 95% CI was 0.754-0.902, the optimal cut-off value was 0.438, the sensitivity was 0.745 and the specificity was 0.783. Conclusions:Under the enhanced recovery after surgery, the risk prediction model established in this study has good effect in predicting postoperative incision infection, which can provide a reference for early identification of high-risk patients with postoperative incision infection after colorectal cancer surgery.

Objective:To investigate the predictive value of different body obesity measures for non-alcoholic fatty liver disease (NAFLD).Methods:It was a cross-sectional study. The present study was a case-control study involving 553 subjects who underwent physical examination from January to April 2022. The subjects were divided into NAFLD group ( n=321 cases) and control group ( n=232 cases) according to abdominal ultrasound imaging parameters. All subjects completed a general information questionnaire, liver ultrasound examination, serum biochemical indices and physical measurements. Logistic regression model was used to analyze the correlation between human obesity measures (neck circumference, triceps skinfold thickness (TSF),body mass index (BMI), waist-to-hip ratio, lipid accumulation index (LAP), visceral fat index (VAI), body roundness index (BRI) and a body shape index (ABSI)) and NAFLD. Receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to evaluate the predictive value of single and combined measures of obesity for NAFLD. Results:The subjects were stratified by gender, and the quartile levels of BMI, neck circumference, TSF, waist-to-hip ratio, LAP, VAI and BRI were all correlated with NAFLD in both male and female (all P<0.05). After further adjustment for confounding factors, compared with those in group Q 1, group Q 4 of the above-mentioned indexes still had higher odds ratios ( P<0.05). The AUC value of LAP in predicting NAFLD was the largest in both men and women, which was 0.836(0.788-0.876) and 0.885(0.839-0.921), and the cut-off value was 41.93 and 33.27, respectively. There was no significant difference in AUC of ROC predicting NAFLD among LAP, BRI and BMI ( P>0.05). The AUC of ABSI in predicting NAFLD was less than 0.7(namely 0.584(0.525-0.641) and 0.679(0.618-0.735) in men and women, respectively), which indicated poor predictive performance for NAFLD. In the pairwise combination index, the AUC of ROC predicting NAFLD with TSF+LAP in male was the largest, which was 0.864(0.819-0.901), and there was statistical significance when compared with BRI (AUC=0.818(0.769-0.860)) and BMI (AUC=0.816(0.767-0.858)) ( P<0.05), but there was no statistical significance when compared with LAP (AUC=0.836(0.788-0.876)) ( P>0.05). The AUC of ROC predicting NAFLD with VAI+LAP in women was the largest, it was 0.894(0.849-0.928), there was statistical significance when compared with BMI (AUC=0.849(0.799-0.890)) ( P<0.05), but there was no statistical significance when compared with LAP (AUC=0.885(0.839-0.921)) and BRI (AUC=0.870(0.822-0.908)) ( P>0.05). Conclusion:BMI, neck circumference, TSF, waist-to-hip ratio, LAP, VAI and BRI all have good predictive value for NAFLD.

Objective : To investigate the role and mechanism of bone formation caused by the ratio of advanced platelet-rich fibrin (A-PRF) and β-tricalcium phosphate (β-TCP) in rabbit femur defect model, which provides a new idea for clinical treatment of bone defect. Methods : Twenty-four New Zealand white rabbits were divided into model group, 1∶1 complex group (A-PRF∶β-TCP=1∶1), 2∶1 complex group (A-PRF∶β- TCP=2∶1) and 4∶1 complex group (A-PRF∶β- TCP=4∶1）, with 6 rabbits in each group. Femoral defect models were constructed in each group. In the composite group, the bone defect was filled with composite material, while in the model group, no material was filled. After 8 weeks, the animals were euthanized and specimens were collected. Bone mineral density (BMD), bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.SP) and trabecular number (Tb.N) in femoral defect tissue were measured by micro-CT and photographed. Hematoxylin - eosin staining was used to detect the pathological changes of new bone tissue. The morphological changes of the new bone tissue were observed by scanning electron microscopy. Determination of phospho-mitogen activated protein kinase p38 (p-p38MAPK), CCAAT/enhancer binding protein homologous protein (CHOP) and phospho-cysteine aspartic protease-3 (p-Caspase3) in newborn femur by ELISA. The mRNA expressions of osteoprotegerin (OPG), bone morphogenetic protein-2 (BMP-2), receptor activator of nuclear factor kappa-B ligand (RANKL) and p38MAPK were detected by real-time quantitative PCR. The expression of OPG, BMP-2, RANKL, p-p38MAPK and p-Caspase3 protein in the new bone tissue was observed by immunohistochemistry. Results : In the model group, bone formation in the femoral defect area was slow and osteogenic quality was poor. Compared with the model group, the bone formation and neocapillaries of femoral defect area in the complex group was good, BMD, BV.TV, Tb.Th, Tb.N were increased, and Tb.Sp were decreased, the expressions of p-p38MAPK, CHOP and p-Caspase3 were decreased, and the mRNA and protein expressions of OPG and BMP-2 were increased. The mRNA expression of RANKL and p38MAPK was decreased. Apoptosis in new bone tissue of each group showed the lowest apoptosis rate in samples of the 2∶1 complex group (P<0.05); A-PRF: β-TCP=2∶1 ratio has the best osteogenic effect. Conclusion The complex composed of A-PRF and β-TCP can promote the expression of OPG, inhibit the expression of RANKL and phosphorylation of p38MAPK, reduce the apoptosis of new bone tissue cells, and promote osteogenic differentiation.

Objective: To understand the status quo and influencing factors of overweight and obesity in preschool children, and to provide scientific theoretical reference for the prevention and control of overweight and obesity in preschool children in Urumqi. Methods: Stratified cluster sampling method was adopted to select 1 897 preschool children from 10 kindergartens in Urumqi from October to December in 2021 to understand the status quo of overweight and obesity of preschool children by measuring their height and weight. The influencing factors were collected by questionnaire survey, including Chinese preschoolers eating behavior questionnaire, Chinese preschooler s caregivers feeding behavior scale, 3-6 year old children s home nurture environment scale, and characteristics and influencing factors of physical activity among preschool children. Results: The prevalence of overweight and obesity in preschool children was 31.21% (592), including 19.50% (370) overweight and 11.70% (222) obesity.Childhood overweight and obesity detection rates varied significantly by age, sex, child dietary habits, father BMI, maternal BMI, and maternal pre pregnancy BMI ( χ 2=19.63,28.75,9.45,18.21,18.45,19.36, P <0.05). Multivariate Logistic regression analysis showed that gender, children s eating habits, paternal BMI, pregnancy BMI, satiety responsiveness, external eating, initiative eating, weight concerns, behavior restricted feeding, physical activity and family physical activity environment were the influencing factors of overweight and obesity in preschool children( OR =0.52,1.43,1.51,1.44,0.69,0.74,1.35,1.71,0.81,0.96,1.10, P < 0.05 ). Conclusion Overweight and obesity in preschool children are popular in Urumqi. Education, diet control and scientific exercise should be strengthened to prevent childhood overweight and obesity.

Objective:To explore the status quo of self-disclosure in patients with stroke and analyze its influencing factors.Methods:In this cross-sectional study, totally 248 patients with stroke admitted in Neurological Wards of a ClassⅢ Grade A hospital in Zhengzhou between February and August 2019 were selected as subjects by convenient sampling, and investigated with the demographic questionnaire, Distress Disclosure Index (DDI) , Stigma Scale for Chronic Illness (SSCI) , Self-rating Depression Scale (SDS) and Perceived Social Support Scale (PSSS) .Results:The SSCI score of the stroke patients was (35.56±6.26) , including 17.74% (44/248) at low levels, 74.60% (185/248) at medium levels, and 7.66% (19/248) at high levels. The total SSCI score of the stroke patients was (55.95±10.80) ; the SDS score was (56.98±9.31) ; and the PSSS score was (54.10±10.83) . Multiple linear regression analysis revealed that educational level, monthly household income per capita, stigma of chronic diseases, depression and social support were the influencing factors of self-disclosure in patients with stroke ( P<0.05) , which could account for 36.6% of the total variation of self-disclosure. Conclusions:The self-disclosure of stroke patients stands at a medium level and needs to be improved. Medical workers should take effective interventions to improve the self-disclosure of stroke patients and promote their physical and mental health.