Objective:To investigate the expression level of long non-coding RNA long stress-induced non-coding transcript 5 (LSINCT5) in cervical squamous cell carcinoma tissues and its effect of regulating miRNA-451(miR-451) on cell proliferation and migration ability of cervical squamous cell carcinoma and the possible mechanism.Methods:Cancer tissues and its adjacent normal tissues (2 cm away from the tumor edge) of 40 patients with cervical squamous cell carcinoma in Shanxi Province Cancer Hospital and Shanxi Bethunn Hospital from January 2023 to June 2023 were collected. Human normal cervical epithelial cell line H8 and cervical squamous cell carcinoma cell lines SiHa and CaSki were selected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of LSINCT5 and miR-451 in cervical cancer tissues, its adjacent tissues and all cell lines. The SiHa cell line with the highest relative expression level of LSINCT5 was selected and transfected with the small interfering RNA targeting LSINCT5 (si-LSINCT5 group) sequence and its negative control sequence (si-Con group), respectively. CCK-8 method was also used to detect the proliferation ability of SiHa cell of both groups. The migration ability of the 2 groups of SiHa cells was detected by using scratch healing assay. Dual luciferase reporter gene assay was used to verify the targeting relationship between LSINCT5 and miR-451. Western blot assay was used to detect the expressions of related proteins in PI3K-AKT-mTOR signaling pathway in the 2 groups.Results:The relative expression level of LSINCT5 in cancer tissues and adjacent tissues of 40 patients with cervical squamous cell carcinoma was 3.25±0.44 and 1.02±0.18, respectively, and the relative expression level in cancer tissues was higher than that in adjacent tissues ( t = 25.69, P < 0.01). The relative expression level of LSINCT5 in normal cervical epithelial cells H8, cervical squamous cell carcinoma cells CaSki and SiHa was 1.00±0.06, 2.41±0.08 and 4.42±0.09, respectively, and the difference was statistically significant ( F = 5.48, P < 0.001). CCK-8 method showed that the proliferation ability of SiHa cells in the si-LSINCT5 group was lower than that in the si-Con group from 24 h after transfection, and the differences were statistically significant (all P < 0.05). The cell scratch healing experiment showed that the cell scratch inhibition rates of the si-LSINCT5 group and the si-Con group were (70±6)% and (34±9)%, respectively, and the differences were statistically significant ( t = 5.76, P < 0.05). Dual luciferase reporter gene assay confirmed that LSINCT5 had a targeting relationship with miR-451. The relative expression levels of p-PI3K, p-Akt, and p-mTOR in si-LSINCT5 group were lower than those in the si-Con group (all P < 0.05). Conclusions:The relative expression level of lncRNA LSINCT5 is high in cervical squamous cell carcinoma tissues. Knockdown of LSINCT5 may inhibit the proliferation and migration ability of cervical squamous cell carcinoma by sponging miR-451 and reducing the expression of related proteins in PI3K-AKT-mTOR signaling pathway.

Objective:To investigate the expression level of long non-coding RNA long stress-induced non-coding transcript 5 (LSINCT5) in cervical squamous cell carcinoma tissues and its effect of regulating miRNA-451(miR-451) on cell proliferation and migration ability of cervical squamous cell carcinoma and the possible mechanism.Methods:Cancer tissues and its adjacent normal tissues (2 cm away from the tumor edge) of 40 patients with cervical squamous cell carcinoma in Shanxi Province Cancer Hospital and Shanxi Bethunn Hospital from January 2023 to June 2023 were collected. Human normal cervical epithelial cell line H8 and cervical squamous cell carcinoma cell lines SiHa and CaSki were selected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of LSINCT5 and miR-451 in cervical cancer tissues, its adjacent tissues and all cell lines. The SiHa cell line with the highest relative expression level of LSINCT5 was selected and transfected with the small interfering RNA targeting LSINCT5 (si-LSINCT5 group) sequence and its negative control sequence (si-Con group), respectively. CCK-8 method was also used to detect the proliferation ability of SiHa cell of both groups. The migration ability of the 2 groups of SiHa cells was detected by using scratch healing assay. Dual luciferase reporter gene assay was used to verify the targeting relationship between LSINCT5 and miR-451. Western blot assay was used to detect the expressions of related proteins in PI3K-AKT-mTOR signaling pathway in the 2 groups.Results:The relative expression level of LSINCT5 in cancer tissues and adjacent tissues of 40 patients with cervical squamous cell carcinoma was 3.25±0.44 and 1.02±0.18, respectively, and the relative expression level in cancer tissues was higher than that in adjacent tissues ( t = 25.69, P < 0.01). The relative expression level of LSINCT5 in normal cervical epithelial cells H8, cervical squamous cell carcinoma cells CaSki and SiHa was 1.00±0.06, 2.41±0.08 and 4.42±0.09, respectively, and the difference was statistically significant ( F = 5.48, P < 0.001). CCK-8 method showed that the proliferation ability of SiHa cells in the si-LSINCT5 group was lower than that in the si-Con group from 24 h after transfection, and the differences were statistically significant (all P < 0.05). The cell scratch healing experiment showed that the cell scratch inhibition rates of the si-LSINCT5 group and the si-Con group were (70±6)% and (34±9)%, respectively, and the differences were statistically significant ( t = 5.76, P < 0.05). Dual luciferase reporter gene assay confirmed that LSINCT5 had a targeting relationship with miR-451. The relative expression levels of p-PI3K, p-Akt, and p-mTOR in si-LSINCT5 group were lower than those in the si-Con group (all P < 0.05). Conclusions:The relative expression level of lncRNA LSINCT5 is high in cervical squamous cell carcinoma tissues. Knockdown of LSINCT5 may inhibit the proliferation and migration ability of cervical squamous cell carcinoma by sponging miR-451 and reducing the expression of related proteins in PI3K-AKT-mTOR signaling pathway.

The IL-8 and MMP-7 genes participate in the carcinogenesis of many malignancies, but the role of both genes in cervical cancer is not fully elucidated. The aim of this study was to determine the frequency of IL-8 and MMP-7 gene mutations and to assess their effects on the risk of early stage cervical cancer and lymph node metastasis. The clinical stage and histological grade of cervical cancer were also studied. The peripheral blood from the patients with early stage cervical cancers and normal controls was collected and the DNA was extracted. The incidence of IL-8 and MMP-7 gene mutations was assessed by using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS PCR) and restriction fragment length polymorphism (RFLP). The data were statistically analyzed by x2 test. The results showed that: (1) The genotype frequency of IL-8 -251AT and TT was significantly higher in the cervical cancer group than in the normal control group (OR=2.290 and 2.619 respectively, P=0.001), and it was also higher in the lymphatic metastasis group than that without metastasis (OR=2.917, P=0.035); (2) The frequency of MMP-7 -181G/G genotype was significantly higher in the cervical cancer group and in the lymphatic metastasis group (P<0.05); (3) The incidence of IL-8 mutation was two times higher in IIa cervical cancer group than in Ib1 and Ib2 cervical cancer group (P=0.006). For the MMP-7 gene, there was statistically significant difference in the incidence of mutation between the Ib1, Ib2 and the IIa (P=0.000); (4) Different histological types and different grades of cervical cancer had different incidence of mutations, statistically. It was suggested that there was significant difference in the genotype of IL-8 -251TT and MMP-7 -181GG polymorphism between the cervical cancer group and the lymph node metastasis group. Moreover, individuals with IL-8 T allele or MMP-7 G allele carriers were at significantly higher risk of cervical cancer, particularly the early (IIa) and medium, poorly differentiated cervical cancer (G2+G3).

The IL-8 and MMP-7 genes participate in the carcinogenesis of many malignancies,but the role of both genes in cervical cancer is not fully elucidated.The aim of this study was to determine the frequency of IL-8 and MMP-7 gene mutations and to assess their effects on the risk of early stage cervical cancer and lymph node metastasis.The clinical stage and histological grade of cervical cancer were also studied.The peripheral blood from the patients with early stage cervical cancers and normal controls was collected and the DNA was extracted.The incidence of IL-8 and MMP-7 gene mutations was assessed by using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS PCR) and restriction fragment length polymorphism (RFLP).The data were statistically analyzed by x2 test.The results showed that:(1) The genotype frequency of IL-8 -251AT and TT was significantly higher in the cervical cancer group than in the normal control group (OR=2.290 and 2.619 respectively,P=0.001),and it was also higher in the lymphatic metastasis group than that without metastasis (OR=2.917,P=0.035); (2) The frequency of MMP-7 -181G/G genotype was significantly higher in the cervical cancer group and in the lymphatic metastasis group (P＜0.05); (3) The incidence of IL-8 mutation was two times higher in Ⅱa cervical cancer group than in Ⅰ bl and Ⅰ b2 cervical cancer group (P=0.006).For the MMP-7 gene,there was statistically significant difference in the incidence of mutation between the Ⅰ bl,Ⅰ b2 and the Ⅱ a (P=0.000); (4) Different histological types and different grades of cervical cancer had different incidence of mutations,statistically.It was suggested that there was significant difference in the genotype of IL-8 -251TT and MMP-7 -181GG polymorphism between the cervical cancer group and the lymph node metastasis group.Moreover,individuals with IL-8 T allele or MMP-7 G allele carriers were at significantly higher risk of cervical cancer,particularly the early ( Ⅱ a) and medium,poorly differentiated cervical cancer (G2+G3).

The mosquito vector of filariasis malayi and its vectorial capacity was investigated In 5 endemic villages in Leshan Prefecture, Sichuan Province. The results showed that the man-biting rate, numan blood index and vectorial capacity of An. lesteri anthropophagus were 0.7, 5.1 and 10.63 times higher than those of An. sinensis. Besides, the natural infection by microfilaria in An, lesteri anthropophagus was also higher than that in An. sinensis by 5 times.From the above result, the authors concluded that An. lesteri anthropophagus was the main vector for transmitting filariasis malayi in the area under study.