Objective To compare the quality of liquids dispensed by intelligent dispensing robot and traditional manual in PIVAS,to ensure the safety of clinical infusion.Methods Using omeprazole and ambroxol as the main drugs and 5％glucose injection and 0.9％sodium chloride solution as the solvent,a number of quality indexes including drug residues and the number of insoluble particles were evaluated in the two modes of manual and robotic dispensing,respectively.Results Under the robot dispensing mode and manual dispensing mode,the residual amount of drugs was less than 5％,the insoluble particles were far less than the limit,the pH value was in line with the human infusion range,and the endotoxin and aseptic culture were negative results,with no statistical significance(P＞0.05).Conclusions The quality of liquid dispensed under the robotic dispensing mode is guaranteed,and there is no significant difference in the quality of liquid dispensed with the traditional manual dispensing mode,which provides a reliable guarantee for the large-scale application of robotic dispensing in intravenous admixture service.

Objective To compare the quality of liquids dispensed by intelligent dispensing robot and traditional manual in PIVAS,to ensure the safety of clinical infusion.Methods Using omeprazole and ambroxol as the main drugs and 5％glucose injection and 0.9％sodium chloride solution as the solvent,a number of quality indexes including drug residues and the number of insoluble particles were evaluated in the two modes of manual and robotic dispensing,respectively.Results Under the robot dispensing mode and manual dispensing mode,the residual amount of drugs was less than 5％,the insoluble particles were far less than the limit,the pH value was in line with the human infusion range,and the endotoxin and aseptic culture were negative results,with no statistical significance(P＞0.05).Conclusions The quality of liquid dispensed under the robotic dispensing mode is guaranteed,and there is no significant difference in the quality of liquid dispensed with the traditional manual dispensing mode,which provides a reliable guarantee for the large-scale application of robotic dispensing in intravenous admixture service.

Adeno-associated virus (AAV)-based gene therapies hold broad application prospects in the treatment of rare and genetic diseases. However, emerging safety concerns such as dose-dependent immune responses, target organ toxicity, and genomic integration have increasingly become apparent, significantly limiting their clinical translation. This article begins by examining the major manifestations of AAV vector toxicity, delvesinto the mechanisms by which factors such as capsid structure, genome design, and expression regulation contribute to toxic responses, and provides a systematic review of the advances in current mainstream detection technologies—including droplet digital PCR, single-cell RNA sequencing, bioluminescence imaging, and liquid biopsy—for toxicity identification and monitoring. Furthermore, it explores mitigation strategies such as capsid engineering, route of administration optimization, immune modulation, and personalized interventions. The paper proposes a conceptual "design-monitor-intervene" closed-loop control framework, aiming to offer valuable insights for safety evaluation, clinical application optimization, and regulatory strategy development of AAV-based gene therapeutics.

Objective:To evaluate and study the current status of safety and quality control of moxifloxacin hydro-chloride and sodium chloride injection.Methods:Fifteen production enterprises nationwide producing moxifloxacin hydrochloride and sodium chloride injection were sampled and tested according to each manufacturer's respective quality standards.Additionally,the safety-related items of"bacterial endotoxins"and"abnormal toxicity"were further studied and expanded upon.The rationality of the existing standards was analyzed.Results:According to the standard test,the qualification rate of 187 batches of moxifloxacin hydrochloride was 100％.However,compa-ring the quality standards used by various enterprises,it was found that there are significant differences in their re-quirements for bacterial endotoxin projects and abnormal toxicity projects.After using a unified and stricter standard for expansion testing,all 187 batches of samples still passed.Conclusion:The overall quality of moxifloxacin hydrochloride and sodium chloride injection in China is satisfactory in terms of safety control.It is recommended to unify the safety standards according to the draft quality standard for"moxifloxacin hydrochloride and sodium chlo-ride injection"issued by the National Pharmacopoeia Commission.

BACKGROUND:Exosomes play a role in all stages of wound repair,and there is currently a large body of research on exosomes in skin wound repair,which has been shown to have great potential for clinical applications. OBJECTIVE:To summarize and discuss the main mechanisms and clinical applications of exosomes in the treatment of skin wounds,in order to promote the clinical translation of exosomes. METHODS:PubMed,clinicaltrials.gov,China National Knowledge Infrastructure,Food and Drug Administration database,and Chinese Clinical Trial Register were searched from inception to March 2023.The English search terms were"exosomes,wound healing,stem cells,chronic wound,immunoregulation,inflammation,skin,therapeutic use,isolation,characterization,infections".The Chinese search terms were"exosomes,wound healing,stem cells,immunomodulation,clinical applications".A total of 79 articles were included for the summary. RESULTS AND CONCLUSION:(1)Exosomes can improve and accelerate wound healing through inflammation regulation,immune protection,angiogenesis,cell proliferation and migration,and collagen remodeling.(2)Exosomes derived from stem cells have mature preparation techniques and related mechanism research,which is currently the mainstream research direction.Non-stem cell-derived exosomes have the advantages of convenience,economy,and easy production,and can be used as a supplement for clinical applications.(3)The clinical application of exosomes is still in its infancy,but has great potential for application.Various exosome modification techniques have laid the foundation for the future development of clinically personalized services and require further research.(4)The clinical translation of exosomes faces many challenges,such as low yield,high heterogeneity,lack of unified standards for isolation,purification,and quality control,and difficulties in storage.

Objective To discuss medicine using regularity of TCM treatment for hypothyroidism. Methods Articles in the CNKI from Jan. 2000 to Dec. 2013 were searched. Articles about TCM treatment for hypothyroidism were screened for the establishment of database. Microsoft Excel was used to analyze the frequency of main medicine, composing principle, and combination of new medicine in the articles. Results Totally 150 cases of prescriptions about hypothyroidism were screened, which involved 178 Chinese herbal medicines. Poria in the prescriptions appears with the highest frequency;Atractylodis Macrocephalae Rhizoma and Poria have the highest frequency of medicinal pair. 14 core combinations and 7 new combinations were obtained through evolution. Conclusion Most doctors’ TCM treatment for hypothyroidism in CNKI is to replenish qi to invigorate the spleen, warm the kidney to tonify yang, and excrete water and dampness.

Objective To establish the fingerprint of amino acids composition of Jinlong capsule. Methods Using 6-acetamido-4-hydroxy-2-methyl quinoline (AQC) as the derivating agent, 17 kinds of amino acids were analyzed by UPLC at the same time and the fingerprint of amino acids composition of Jinlong capsule was established. Result Totally 22 co-possessing peaks were selected as the fingerprint peaks, 17 kinds of amino acids were identified. The fingerprint similarity value of 10 batches was above 0.9. Conclusion The fingerprint is characteristic and can be used for the quality control of Jinlong capsule.