Physicochemical Characterization and Biocompatibility of Bioactive Glass/Polycaprolactone Scaffold for Alveolar Bone Regeneration In bone tissue engineering, scaffold-based approaches offer great potential for regenerating damaged or lost bone tissue. Composite scaffolds have gained popularity because they combine the beneficial properties of two or more materials, providing promising biomimetic properties for tissue regeneration. In our study, combining the biocompatibility of bioactive glass with the mechanical qualities of polycaprolactone provides tailored properties and scaffold architecture conducive to alveolar bone regeneration. This study aims to develop a bone substitute using bioactive glass/polycaprolactone (BG/PCL) composites and to evaluate its biocompatibility for potential applications in alveolar bone regeneration. BG/PCL scaffolds were fabricated using a solvent-casting method at three different weight percentages (wt.%) ratios (10:90, 20:80, and 30:70). The scaffolds were characterised using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and methyl tetrazolium (MTT) (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) Assay to evaluate their physical, chemical, and biocompatibility properties. Among the three BG:PCL composite ratios tested (10:90, 20:80, and 30:70), the 10:90 ratio demonstrated the most favourable properties. SEM analysis of this composition revealed optimal surface roughness and well-distributed interconnected pores, which are conducive to cell attachment, enhanced cellular infiltration, and efficient nutrient diffusion. FTIR spectra displayed characteristic absorption bands corresponding to the functional groups of BG and PCL components while the biocompatibility test confirmed the viability and proliferation of periodontal ligament (PDL) fibroblast cells when cultured with the scaffolds. This study demonstrates that the developed BG/PCL composite, especially at the 10:90 ratio, possesses suitable properties and biocompatibility, highlighting its potential for use in alveolar bone regeneration.

Understanding the Effects of Sterilisation Methods on Orthodontic Archwires Properties: A Review Sterilisation is the removal of all microorganisms, vegetative or spore-forming, from an article, surface or medium that can be accomplished through steam autoclaving, dry heat and cold solution following strict guidelines for efficient archwire sterilisation. Orthodontic archwire sterilisation is a concern because patients are often exposed to contaminated dental products. Thus, new and used arch wires will not show significant differences in terms of properties while in usage. This article presents a review of the sterilisation methods effects on the characteristics and properties of several types of orthodontic archwires including stainless steel (SS), nickel-titanium (NiTi), copper NiTi, beta titanium (β-Ti), heat-activated NiTi and titanium molybdenum (TiMb). Available records in “all databases” of Web of Science, PubMed, ScienceDirect, Scopus and Elsevier were searched, and other studies were manually searched using keywords, retrieved and compiled. Studies related to sterilisation of orthodontic archwires such as archwire type, sterilisation method, characterisation techniques and mechanical properties were summarised based on articles published from 1st July 2015 to 29th February 2024, and it is shown that the characteristics of orthodontic archwires may be affected by sterilisation techniques. Heat sterilisation can change the load-deflection properties of some wires, while the chemical composition and surface structures of archwires are unchanged. After sterilisation, the number of nickel ions released from the archwires does not increase significantly. The findings support the idea that archwire sterilisation procedures are an effective infection control treatment and facilitate the mechanical characterisation of orthodontic archwires.

Chrysanthemum rubellum (durian) flower (CR) is well-known for its usefulness in conventional and advanced medicine. Bioactive glass properties are geared towards hard tissue regeneration. Hence, this study aims to investigate the response of dental pulp stem cells (DPSC) when exposed to bioactive glass-chrysanthemum flower extract-conditioned medium. Methods: Chrysanthemum rubellum (durian) yellow coloured petals freeze dried extracts (CRE) was prepared by separation and agitation in distilled water where the final powdery compound was being investigated and combined with melt-derived BG 45S5 powder to produce BGCRE-conditioned medium. The CRE extracts in various concentrations and BGCRE-conditioned medium were exposed to DPSC and the cells responses were assessed using AB and MTT assays. The CRE and BGCRE-conditioned media were also assessed using ICP-OES to check for ionic release profile from both medium. Results: The CRE-conditioned medium (7.81, 15.63, 31.25 and 62.5 μg/ml) showed a dose-dependent effect towards DPSC from Days 1 until 14. The BGCRE-conditioned medium containing BG powders (1 mg/ml) with the lower amount of CRE extracts (0.02 and 0.1 mg/ml) promoted DPSC viability and proliferation rate from Days 1 until 14 based on AB and MTT assays. The BGCRE-conditioned medium has potentially affected the DPSC viability and proliferation. Conclusion: The presence of CRE in BGCRE-conditioned medium enhanced the DPSC viability and proliferation possibly through the combined effect of CRE and BG. This BGCRE combination showed potential as natural medicament for dental tissue regeneration
Craniofacial and Biomaterials Sciences Cluster ; Advanced Medical & ; Dental Institute ; Universiti Sains Malaysia ; Bertam ; 13200 Kepala Batas ; Pulau Pinang ; Malaysia 2 Department of Paedodontics and Preventive Dentistry ; Coorg Institute of Dental Sciences ; Virajpet ; 571218 ; Coorg Institute ; Karnataka State ; India 3 Faculty of Applied Sciences ; Universiti Teknologi MARA ; 40450 Shah Alam ; Selangor ; Malaysia

Introduction: The interpretations of printed panoramic radiographs film frequently depend on personal judgements analysis by observers. The findings correlations between more than two observers must consider statistical tool which can determine that sometimes the observers will agree or disagree merely by chance. Intraclass Correlation Coefficient (ICC) generally measures the degree of similarity among individuals within a cluster. Kappa statistic is the most frequently applied statistical analysis for this reason. This study aims to determine the validity of utilising printed panoramic radiographs for assessing dental anomalies and inter-examiner reliability in detecting dental anomalies observed within the panoramic radiographs. Methods: Twenty samples of printed versions of orthopantomogram (OPG) were arbitrarily selected from the AMDI Dental Clinic. Radiographic analyses were conducted twice, Trial 1 and Trial 2, with two-week intervals in between. Ten (10) printed OPG films were used in each session. During both assessments, incidence of dental anomalies was determined. All twenty OPGs were examined and analysed by the principal investigator and two independent examiners according to the inclusion and exclusion criterias. The data analysis was carried out using SPSS version 23. Results: The results of ICC for Trials 1 and 2 were 0.961 and 0.984, respectively. Conclusion: The outcomes demonstrated that the printed OPG films can be used to detect dental anomalies and inter-examiner dependability was in very good assertion for both trials, hence printed OPG films that satisfy the inclusion and exclusion criterias can be utilised for assessing dental anomalies as shown by the reliable ICC values in this study.

Panoramic radiograph is a routine investigation tool in orthodontic diagnosis and treatment planning however the limitation of two-dimensional image in assessing the position of impacted canine and surrounding tissues had cause unnecessary treatment options and complications during treatment. This report highlights the differences in investigation outcome and orthodontic treatment planning of two similar cases of patients with impacted upper canine, in which one only had panoramic radiograph and another case supplemented with Cone Beam Computed Tomography (CBCT). The case supported only by panoramic radiograph had changes in treatment planning during canine removal surgery as prevention from oro-antral fistula. This event could have been prevented if CBCT was taken where the canine can be assessed for proximity towards maxillary sinus. It is recommended for CBCT in orthodontic assessment for canine impaction especially for cases where canine is positioned higher than root apex of adjacent teeth and when root resorption is suspected.

Dental treatment under general anaesthesia may be needed for some children and adolescents due to medical or behaviour problem. The objective of the study is to identify the type of treatment that has been carried out under GA in Hospital Universiti Sains Malaysia (HUSM). A retrospective record review study from hospital records of dental patients (under 18 years old) receiving dental treatment under GA from 2003 until 2007 were retrieved from the database. Information such as the reason for GA, and the type of treatment provided was recorded in data sheet. The data were analyzed using SPSS 12.0.1 for Windows. It was checked and verified for errors. A total of 349 cases were treated of which 43.6% had medical problems. Patients were mostly diagnosed to have rampant caries (77.1%) and some of them have behavioural problems (34.4%). Treatment pattern in deciduous dentition revealed more extraction (97.8%) as compared to restoration (75.7%) whereas in permanent dentition more restoration was done (24.3%) as compared to extraction (2.2%). Majority of the restorations were done using Glass Ionomer Cements (47.5%). Biopsy (4.3%) contributed mainly to the surgery (24.1%) done during GA. General anesthesia is necessary when dental disease is interfering with health and general well-being of patient and it can facilitated dental treatment allowing dentists to benefit from improved treatment conditions and provide a higher quality of care.