Objective To promote the application of the best evidence for supportive care in the management of depression in patients with head and neck cancer, and to improve the quality of clinical care.Methods Follow the three stages of clinical evidence practice of Joanna Briggs Institute (JBI): baseline review, practice change and effect evaluation, the best evidence of supportive nursing in depression management for head and neck cancer patients were retrieved, and 4 quality review indicators were developed. A total of 50 hospitalized head and neck cancer patients and 12 nurses were selected to collect data by means of field observation, interviews and nursing records. The obstacles to the application of evidence were analyzed and a practical scheme was worked out.Results The nurse's implementation rate of the four review indicators increased from 0-90％ at the baseline review to 90％-100％ after the evidence was applied. Except for Indicator 3 "Indicators nurses introduced the pressure on patients and their families to face cancer", the implementation rates of the other three indicators were significantly improved (P<0.01). After the application of evidence, the incidence of depression was decreased (P<0.05). The score of Relaxation, Sleep and Fatigue Management Knowledge Questionnaire was (18.20±14.09) before the application of evidence, which was lower than that after the application of evidence (80.60±12.84), and the difference was statistically significant (t=-23.14, P<0.01). The nurses' score of Supportive Nursing Knowledge Questionnaire before the application of evidence was (63.83±10.78), which was lower than that after the application of evidence (95.5±3.18), and the difference was statistically significant (t=-9.76,P<0.01).Conclusions The evidence-based application program can standardize nurses' supportive nursing behaviors for patients with depressive symptoms, reduce the severity of depression, improve the self-support knowledge level, and improve the quality of depression management in hospitalized patients with head and neck cancer.

Objective To observe the effect of hyperbaric oxygen (HBO) combined with fasudil hydrochloride on the nerve function of the patients after embolization in the treatment of intracranial aneurysm.Methods Clinical data of 130 patients with intracranial aneurysm treated in our hospital from June 2011 to June 2016 were recruited as research subjects and they were divided into the control group (n =55) and the observation group (n =75) in accordance with different treatment methods.The patients of the control group were treated with nimodipine in addition to routine therapy,while the patients of the observation group were supplemented with HBO on the basis of the treatment received by the control group.Brain edema scores both before and after treatment were compared between the 2 groups.Analyses were also made on the following indexes:Glasgow coma scale (GCS) and Glasgow outcome scale (GOS) grade,middle cerebral artery blood (MCA) flow velocity and cerebral vasospasm (CVS),neural function defect (NFD)scores,Barthel index scores,incidence of cerebral infarction at day 30 after surgery,as well as incidence of adverse reactions.Results There was statistical significance in the brain edema scores,NFD scores and Barthel scores of the observation group,as compared with those before treatment (P ＜ 0.01).MCA and CVS blood flow velocity was significantly lower than that before treatment (P ＜ 0.01),and that of the observation group was obviously lower than that of the control group (P ＜ 0.05).The GCS scores were significantly lower than those before treatment [(14.14±1.88) vs (8.87±1.06),(11.17±1.71) vs (8.71±1.17),P＜0.01].Three months after treatment,GOS grade of the observation group was obviously superior to that of the control group (F =9.625,P ＜0.01).The rate of cerebral infarction in the patients of the observation group was significantly lower than that of the control group (6.67％ vs 18.18％,P ＜ 0.05),and statistical significance could be noted,when comparisons were made between the 2 groups (P ＜ 0.05).Conclusions HBO combined with fasudil hydrochloride in the treatment of intracranial aneurysm after embolization could significantly improve the neurological function,enhance cerebral artery blood flow velocity and promote recovery of daily activities of the patients.

Objective To observe the effect of hyperbaric oxygen (HBO) combined with fasudil hydrochloride on the nerve function of the patients after embolization in the treatment of intracranial aneurysm.Methods Clinical data of 130 patients with intracranial aneurysm treated in our hospital from June 2011 to June 2016 were recruited as research subjects and they were divided into the control group (n =55) and the observation group (n =75) in accordance with different treatment methods.The patients of the control group were treated with nimodipine in addition to routine therapy,while the patients of the observation group were supplemented with HBO on the basis of the treatment received by the control group.Brain edema scores both before and after treatment were compared between the 2 groups.Analyses were also made on the following indexes:Glasgow coma scale (GCS) and Glasgow outcome scale (GOS) grade,middle cerebral artery blood (MCA) flow velocity and cerebral vasospasm (CVS),neural function defect (NFD)scores,Barthel index scores,incidence of cerebral infarction at day 30 after surgery,as well as incidence of adverse reactions.Results There was statistical significance in the brain edema scores,NFD scores and Barthel scores of the observation group,as compared with those before treatment (P ＜ 0.01).MCA and CVS blood flow velocity was significantly lower than that before treatment (P ＜ 0.01),and that of the observation group was obviously lower than that of the control group (P ＜ 0.05).The GCS scores were significantly lower than those before treatment [(14.14±1.88) vs (8.87±1.06),(11.17±1.71) vs (8.71±1.17),P＜0.01].Three months after treatment,GOS grade of the observation group was obviously superior to that of the control group (F =9.625,P ＜0.01).The rate of cerebral infarction in the patients of the observation group was significantly lower than that of the control group (6.67％ vs 18.18％,P ＜ 0.05),and statistical significance could be noted,when comparisons were made between the 2 groups (P ＜ 0.05).Conclusions HBO combined with fasudil hydrochloride in the treatment of intracranial aneurysm after embolization could significantly improve the neurological function,enhance cerebral artery blood flow velocity and promote recovery of daily activities of the patients.

OBJECTIVETo provide mutation analysis and prenatal diagnosis for a family affected with congenital factor VII(FVII) deficiency.

METHODSDNA was extracted from peripheral blood samples from the proband and his parents. All exons and flanking sequence of the FVII gene were amplified with PCR and subjected to direct sequencing. Prenatal diagnosis was performed by amniocentesis.

RESULTSA homozygous mutation (NM_000131.3) c.572-1G>A was identified in the proband. Both parents of the fetus were carriers of the mutation.

CONCLUSIONA method for molecular diagnosis of congenital factor VII deficiency was established and successfully applied for an affected family.

Objective The aim of this study was to compare recent clinical efficacy between a novel guidance method for percutaneous pedicle screw placement and the conventional fluoroscopic method for long segments thoracolumbar vertebral fracture without nerve injury. Methods A total of 38 patients with thoracolumbar vertebral fracture in our hospital from September 2010 to December 2012 were divided into group A and group B.Eighteen patients in group A underwent 200 percutaneous pedicle screw fixation by conventional fluoroscopic meth-od.Twenty patients in group B underwent 210 percutaneous pedicle screw fixation by a novel guidance method.All the operation for 38 cases were performed by the same surgeon.The time of insertion,radiation exposure,and accuracy of the screw placement between the two groups were compared.The accuracy of screws was evaluated and graded by two consecutive postoperative CT of operation segment for two groups. Results The mean time for a single pedicle screw placement was (13.11 ±2.32)minutes in group A and (10.35 ±1.92)minutes in group B,respectively.The average radiation exposure was (8.11 ±1.15)s in group B and (13.07 ±2.06)s in group A respectively.The differ-ences were statistically significant for both screw placement and radiation exposure times (P <0.05).A total of 172 screws (86.00%)in group A and 185 screws (88.09%)in group B were perfectly located within the pedicle.The statistical difference of grade A between two groups was not significant(P >0.05).Conclusion The novel guidance system can significantly reduce the insertion time and radiation expo-sure for long segments percutaneous pedicle screw placement,which provides the same accuracy for screw placement compared with the con-ventional method.

Objective To investigate the effects of hyperbaric oxygen (HBO) on intracephalic angiogenesis in rats with intracerebral hemorrhage (ICH) and the mechanism involved.Methods The healthy SD rats were randomly divided into the sham surgery group (group A),the intracerebral hemorrhage group (group B) and the HBO group (group C).Observations were made on changes in rat behavior of each group.Expression levels of proliferating cell nuclear antigen (PCNA),yon Willebrand 's factor (vWF),hypoxia inducible factor-1 (HIF-1 α) and vascular endothelial growth factor (VEGF) were detected in the brain of rats of all the groups.Results Significant differences could be noted in behavior scores of the HBO group at d 14,d 21 and d 28,as compared with those of group B,with obvious statistical significance (P ＜ 0.01).The expression of HIF1-α in the brain tissue around the hematoma was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups (P ＜ 0.01).The expression of PCNA in the brain tissue around the hematoma for the animals of the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜ 0.05).The expression of VEGF for the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜0.01).The expression of vWF for the HBO group was also higher than that of group B,and statistical significance could also be seen in the expression levels at d 14,d 21 and d 28 (P ＜ 0.01).Conclusions HBO could accelerate angiogenesis of the damaged tissue induced by cerebral hemorrhage,promote absorption of hematoma,enable recovery of behavioral capacity and enhance rapid rehabilitation of the body,through expressions of such related factors as HIF-1 a,VEGF,PCNA and vWF proteins.

Objective To investigate the effects of hyperbaric oxygen (HBO) on intracephalic angiogenesis in rats with intracerebral hemorrhage (ICH) and the mechanism involved.Methods The healthy SD rats were randomly divided into the sham surgery group (group A),the intracerebral hemorrhage group (group B) and the HBO group (group C).Observations were made on changes in rat behavior of each group.Expression levels of proliferating cell nuclear antigen (PCNA),yon Willebrand 's factor (vWF),hypoxia inducible factor-1 (HIF-1 α) and vascular endothelial growth factor (VEGF) were detected in the brain of rats of all the groups.Results Significant differences could be noted in behavior scores of the HBO group at d 14,d 21 and d 28,as compared with those of group B,with obvious statistical significance (P ＜ 0.01).The expression of HIF1-α in the brain tissue around the hematoma was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups (P ＜ 0.01).The expression of PCNA in the brain tissue around the hematoma for the animals of the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜ 0.05).The expression of VEGF for the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜0.01).The expression of vWF for the HBO group was also higher than that of group B,and statistical significance could also be seen in the expression levels at d 14,d 21 and d 28 (P ＜ 0.01).Conclusions HBO could accelerate angiogenesis of the damaged tissue induced by cerebral hemorrhage,promote absorption of hematoma,enable recovery of behavioral capacity and enhance rapid rehabilitation of the body,through expressions of such related factors as HIF-1 a,VEGF,PCNA and vWF proteins.

Objectives To observe the effects of varied doses of L-thyroxin sodium ( L-T4) on congenital hypothyroidism ( CH) and to determine appropriate dose for early therapeutic intervention. Methods Totally, 108 cases of confirmed CH were divided into two groups (54 in each group). All the cases were administered with L-T4 at varied doses( 10. 1? - 15. 0 ?g ? kg-1? d-1 for large-dose group and 6. 0 - 10. 0 ?g ?kg-1? d-1 for small-dose group) after their parents were trained for basic knowledge of CH by specialists. Their serum levels of T3, T4 and TSH, as well as their body height, weight and head circumference, were measured during follow-up. L-T4 was maintained at doses of 1. 0 - 4. 0 ?g ? kg -1 ? d-1 when their serum levels of T3, T4 and TSH returned to normal. Results All the 108 cases had their clinical symptoms quickly improved and development and growth accelerated after L-T4 substitution. Their serum level of T3 returned to normal in all cases, that of T4 did not return to normal in six cases of large-dose group and in 16 cases of small-dose group, and that of TSH did return to normal in 22 cases of large-dose group and in 39 cases of small-dose group, respectively, after one-month treatment, with a significant difference between the two groups. Cases in large-dose group gained more weight in average than those in small-dose group when they were three months old. Conclusions Early therapeutic intervention for babies with CH with L-thyroxin sodium of 10. 1 -15. 0 ?g ? kg-1? d-1 could quickly correct abnormal function of their thyroid and could avoid abnormal development of their intelligence due to CH.