In order to explore the role of BspE protein in Brucella infection,yeast two-hybrid tech-nique was used to screen host cell proteins that interact with BspE protein.The constructed BspE recombinant plasmid pGBKT7-BspE was used as bait plasmid to hybridize with the RAW264.7-cD-NA library of mouse mononuclear macrophages by yeast two-hybridization technique.The positive clones were extracted by plasmid,sequenced and co-immunoprecipitation to determine the host cell proteins that could interact with BspE.The subcellular localization of BspE proteins was analyzed by confocal laser microscopy.The physical and chemical properties,protein structure and function of BspE interacting proteins were analyzed by bioinformatics.The siRNA for one of the BspE inter-acting proteins was synthesized,the expression of its gene was silenced in HEK293T cells,and the silenced cells was infected with Brucella M5-90 and the number of intracellular bacteria was coun-ted.The results showed that the decoy plasmid pGBKT7-BspE was successfully constructed,and the plasmid could express BspE protein in yeast.Eight positive clones were obtained from the host cell genome library by yeast two-hybridization.The positive clones were identified as RBM27 and PCBP1 by sequencing,backcross and co-immunoprecipitation.Bioinformatics was used to predict the cell location,protein structure and amino acid composition of RBM27 and PCBP1.After siRNA interference,the expression level of PCBP1 was significantly decreased and the amount of M5-90 in the cell was increased.Brucellosis secreted protein BspE interacts with host proteins RBM27 and PCBPl,and PCBP1 negatively regulates the proliferation of Brucellosis.

Objective:To study the inhibitory effect of endothelial progenitor cells (EPCs) on aortic injury in mice with systemic lupus erythematosus (SLE) arteriosclerosis.Methods:APOE -/- mice were injected with norphytane and high fat diet to establish lupus vascular injury model. Then the mice were divided into normal control group (ND group), high fat diet group (HFD group), high fat diet+ SLE vascular injury group (HFD+ SLE group), high fat diet+ SLE vascular injury+ hydroxychloroquine treatment group (HFD+ SLE+ Hydro group), high fat diet+ SLE vascular injury+ EPCs treatment group (HFD+ SLE+ EPCs group). At the end of the experiment, urine, blood and aortic tissues of mice in each group were collected, and the content of urinary protein and the depth of serum type I interferon (IFN-Ⅰ) were detected by enzyme linked immunosorbent assay (ELISA). The activation of cyclic guanosine monophosphate synthase/interferon gene stimulating factor/type I interferon (cGAS/STING/IFN-Ⅰ) pathway, the levels of inflammatory factors, adhesion fractions and chemokines in the aorta of mice in each group were detected by immunohistochemistry and Western blotting (WB). The lipid deposition in the aorta was detected by oil red staining. Results:The results of ELISA showed that the levels of urinary protein and serum IFN-Ⅰ in HFD+ SLE group were higher than those in normal control group. EPCs treatment could reduce the levels of urinary protein and serum IFN-Ⅰ in SLE atherosclerotic mice. WB results showed that the expression of CD19, CD68, CD34, chemokine, cGAS, p-STING, phosphorylated TANK binding kinase 1 (p-TBK1), phosphorylated interferon regulatory factor 3 (p-IRF3) and IFN-Ⅰ increased in HFD+ SLE group, and hydroxychloroquine and EPCs decreased the levels of these factors. CGAS/STING/IFN-Ⅰ signal pathway is involved in the occurrence and development of atherosclerosis in SLE patients; both EPCs and hydroxychloroquine can inhibit the activation of cGAS/STING/IFN-Ⅰ signal, thus reducing atherosclerosis in SLE mice.Conclusions:cGAS/STING/IFN-Ⅰ pathway is involved in the development of SLE atherosclerosis. EPCs can inhibit the activation of cGAS/STING signal, reduce the expression and secretion of IFN-Ⅰ, and then reduce vascular inflammation and inhibit the development of SLE-related atherosclerosis.

Objective:To study the inhibitory effect of endothelial progenitor cells (EPCs) on aortic injury in mice with systemic lupus erythematosus (SLE) arteriosclerosis.Methods:APOE -/- mice were injected with norphytane and high fat diet to establish lupus vascular injury model. Then the mice were divided into normal control group (ND group), high fat diet group (HFD group), high fat diet+ SLE vascular injury group (HFD+ SLE group), high fat diet+ SLE vascular injury+ hydroxychloroquine treatment group (HFD+ SLE+ Hydro group), high fat diet+ SLE vascular injury+ EPCs treatment group (HFD+ SLE+ EPCs group). At the end of the experiment, urine, blood and aortic tissues of mice in each group were collected, and the content of urinary protein and the depth of serum type I interferon (IFN-Ⅰ) were detected by enzyme linked immunosorbent assay (ELISA). The activation of cyclic guanosine monophosphate synthase/interferon gene stimulating factor/type I interferon (cGAS/STING/IFN-Ⅰ) pathway, the levels of inflammatory factors, adhesion fractions and chemokines in the aorta of mice in each group were detected by immunohistochemistry and Western blotting (WB). The lipid deposition in the aorta was detected by oil red staining. Results:The results of ELISA showed that the levels of urinary protein and serum IFN-Ⅰ in HFD+ SLE group were higher than those in normal control group. EPCs treatment could reduce the levels of urinary protein and serum IFN-Ⅰ in SLE atherosclerotic mice. WB results showed that the expression of CD19, CD68, CD34, chemokine, cGAS, p-STING, phosphorylated TANK binding kinase 1 (p-TBK1), phosphorylated interferon regulatory factor 3 (p-IRF3) and IFN-Ⅰ increased in HFD+ SLE group, and hydroxychloroquine and EPCs decreased the levels of these factors. CGAS/STING/IFN-Ⅰ signal pathway is involved in the occurrence and development of atherosclerosis in SLE patients; both EPCs and hydroxychloroquine can inhibit the activation of cGAS/STING/IFN-Ⅰ signal, thus reducing atherosclerosis in SLE mice.Conclusions:cGAS/STING/IFN-Ⅰ pathway is involved in the development of SLE atherosclerosis. EPCs can inhibit the activation of cGAS/STING signal, reduce the expression and secretion of IFN-Ⅰ, and then reduce vascular inflammation and inhibit the development of SLE-related atherosclerosis.

Objective:To study the effects of electromagnetic radiation（EMR）on the nervous system of rats in a simulated warship environment，so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups，with six rats in each group. There were four exposure groups：60 V/m exposure for 1 d and 3 d，120 V/m exposure for 1 d and 3 d. There were two control groups：control group for 1 d and control group for 3 d. After exposure，cholinergic neurotransmitters，blood-brain barrier permeability，and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group［（515.52±5.88）pmol/L］was lower than that in the control group for 3 d［（550.94±20.44）pmol/L］，with statistically significant difference（ P<0.05）. The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group［（578.84±25.14）pmol/L］and the 120 V/m exposure for 1 d group［（519.62±13.09）pmol/L］were both lower than that in the control group for 1 d［（605.13±17.99）pmol/L］，with statistically significant differences（ P<0.05； P<0.01）. The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group［（586.20±12.20）pmol/L］and the 120 V/m exposure for 3 d group［（591.22±9.78）pmol/L］were both lower than that in the control group for 3 d［（623.68±15.07）pmol/L］，with statistically significant differences（ P<0.01）. The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups（ P<0.01），respectively；the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups（ P<0.01）；after radio frequency（RF）exposure，the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m，the cholinergic neurotransmitters in the brains of SD rats have changed，the permeability of blood-brain barrier is increased，and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats，suggesting that the EMR of this band can bring potential harm to the deck crew，which demands appropriate protective measures.

Objective:To study the effects of electromagnetic radiation（EMR）on the nervous system of rats in a simulated warship environment，so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups，with six rats in each group. There were four exposure groups：60 V/m exposure for 1 d and 3 d，120 V/m exposure for 1 d and 3 d. There were two control groups：control group for 1 d and control group for 3 d. After exposure，cholinergic neurotransmitters，blood-brain barrier permeability，and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group［（515.52±5.88）pmol/L］was lower than that in the control group for 3 d［（550.94±20.44）pmol/L］，with statistically significant difference（ P<0.05）. The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group［（578.84±25.14）pmol/L］and the 120 V/m exposure for 1 d group［（519.62±13.09）pmol/L］were both lower than that in the control group for 1 d［（605.13±17.99）pmol/L］，with statistically significant differences（ P<0.05； P<0.01）. The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group［（586.20±12.20）pmol/L］and the 120 V/m exposure for 3 d group［（591.22±9.78）pmol/L］were both lower than that in the control group for 3 d［（623.68±15.07）pmol/L］，with statistically significant differences（ P<0.01）. The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups（ P<0.01），respectively；the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups（ P<0.01）；after radio frequency（RF）exposure，the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m，the cholinergic neurotransmitters in the brains of SD rats have changed，the permeability of blood-brain barrier is increased，and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats，suggesting that the EMR of this band can bring potential harm to the deck crew，which demands appropriate protective measures.

Objective:To find an effective statistical analysis model for analyzing the detected concentration of the cabin airborne microorganism, so as to provide a method to obtain the theoretical limits of allowable concentration of cabin airborne microbial pollution.Methods:According to the principles of mathematical statistics, the detected data were processed by five mapping transformations: linear transformation y= x, negative exponential transformation y=1/ x, root exponential transformation y=sqrt( x), logarithmic transformation y=log10( x), and inverse trigonometric function transformation y=arctg( x). The distribution of data was analyzed by six probability function models: normal distribution, gamma distribution, exponential distribution, Rayleigh distribution, Weibull distribution, and beta distribution; when the mapping transformation confidence level α=0.05, Kolmogorov-Smirnov test (KS test) was used to test the acceptability of the model. When P≥α, the distribution model was deemed as "not to be rejected" . Results:After linear transformation, negative exponential transformation, root exponential transformation, and logarithmic transformation, the detected concentrations of the cabin airborne microorganism of large surface warships and submarines accepted normal distribution model, gamma distribution model, and Weibull distribution model ( P≥α), while that in the ordinary surface warships accepted Weibull distribution model ( P≥α). Conclusion:Weibull distribution model has good generality in the statistical analysis of the detected concentration of the cabin airborne microorganism, which can be used to calculate the theoretical parameters of allowable concentration.

Objective:To find an effective statistical analysis model for analyzing the detected concentration of the cabin airborne microorganism, so as to provide a method to obtain the theoretical limits of allowable concentration of cabin airborne microbial pollution.Methods:According to the principles of mathematical statistics, the detected data were processed by five mapping transformations: linear transformation y= x, negative exponential transformation y=1/ x, root exponential transformation y=sqrt( x), logarithmic transformation y=log10( x), and inverse trigonometric function transformation y=arctg( x). The distribution of data was analyzed by six probability function models: normal distribution, gamma distribution, exponential distribution, Rayleigh distribution, Weibull distribution, and beta distribution; when the mapping transformation confidence level α=0.05, Kolmogorov-Smirnov test (KS test) was used to test the acceptability of the model. When P≥α, the distribution model was deemed as "not to be rejected" . Results:After linear transformation, negative exponential transformation, root exponential transformation, and logarithmic transformation, the detected concentrations of the cabin airborne microorganism of large surface warships and submarines accepted normal distribution model, gamma distribution model, and Weibull distribution model ( P≥α), while that in the ordinary surface warships accepted Weibull distribution model ( P≥α). Conclusion:Weibull distribution model has good generality in the statistical analysis of the detected concentration of the cabin airborne microorganism, which can be used to calculate the theoretical parameters of allowable concentration.

Objective:To study the inactivation efficacy of pulsed light with different irradiation intensities on microorganisms, so as to provide a validation for the design of sterilization techniques.Methods:Using Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC9372) as experimental bacteria. The carrier quantitative germicidal test was used to evaluate the inactivation efficacy. The exponential dilution method and the pour plate method were used to measure the inactivation efficacy. The scanning electron microscope and transmission electron microscope were used to observe the effects of different irradiation intensities of light on bacterial structure. The bacterial control group (CO group) and the bacterial test group (T group) were set up with the same initial concentration in the experiment. In the CO group, the bacteria without pulsed light irradiation were cultured in parallel for 30 s, 60 s, and 90 s. In the T group, bacteria received irradiation 50 cm, 100 cm, 150 cm, and 200 cm away from the pulsed light source respectively, and the irradiation time duration was set as 30 s, 60 s, and 90 s for each distance.Results:The inactivation rate of ATCC25923 was 100% and that of ATCC9372 was 99.9% when they were 50 cm and 100 cm away from the pulsed light source for 30 s. When the irradiation distance was increased to 150 cm, the inactivation rates of ATCC25923 for 30 s, 60 s, and 90 s were 81.9%, 95.4%, and 100%, respectively; and those of ATCC9372 were 76.0%, 92.3%, and 99.9%, respectively. When the irradiation distance was increased to 200 cm, the inactivation efficiency of pulsed light was significantly reduced; the inactivation rates of ATCC25923 at the three detection time points were reduced to 24.5%, 38.8%, and 48.8%, respectively, and those of ATCC9372 were reduced to 14.2%, 20.0%, and 28.5%.Conclusion:Pulsed light can quickly eradicate microorganisms, and its inactivation rate and efficiency are closely related to the intensity of irradiation.

Objective:To study the inactivation efficacy of pulsed light with different irradiation intensities on microorganisms, so as to provide a validation for the design of sterilization techniques.Methods:Using Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC9372) as experimental bacteria. The carrier quantitative germicidal test was used to evaluate the inactivation efficacy. The exponential dilution method and the pour plate method were used to measure the inactivation efficacy. The scanning electron microscope and transmission electron microscope were used to observe the effects of different irradiation intensities of light on bacterial structure. The bacterial control group (CO group) and the bacterial test group (T group) were set up with the same initial concentration in the experiment. In the CO group, the bacteria without pulsed light irradiation were cultured in parallel for 30 s, 60 s, and 90 s. In the T group, bacteria received irradiation 50 cm, 100 cm, 150 cm, and 200 cm away from the pulsed light source respectively, and the irradiation time duration was set as 30 s, 60 s, and 90 s for each distance.Results:The inactivation rate of ATCC25923 was 100% and that of ATCC9372 was 99.9% when they were 50 cm and 100 cm away from the pulsed light source for 30 s. When the irradiation distance was increased to 150 cm, the inactivation rates of ATCC25923 for 30 s, 60 s, and 90 s were 81.9%, 95.4%, and 100%, respectively; and those of ATCC9372 were 76.0%, 92.3%, and 99.9%, respectively. When the irradiation distance was increased to 200 cm, the inactivation efficiency of pulsed light was significantly reduced; the inactivation rates of ATCC25923 at the three detection time points were reduced to 24.5%, 38.8%, and 48.8%, respectively, and those of ATCC9372 were reduced to 14.2%, 20.0%, and 28.5%.Conclusion:Pulsed light can quickly eradicate microorganisms, and its inactivation rate and efficiency are closely related to the intensity of irradiation.

Objective: To investigate the current situation of risk assessment of neonatal pressure injury (PI) and using of the assessment scale from 8 hospitals in Zhejiang Province,providing reference for further improvement of the scale. Methods: A cross-sectional study was used to investigate 184 nurses in the department of neonatal intensive care unit admitted to 8 hospitals in Zhejiang Province by using a self-made questionnaire. Results: Three of the eight hospitals did not use scale for neonatal PI assessment routinely. Of the remaining five hospitals, three hospitals used Neonatal Skin Risk Assessment Scale (NSRAS), one hospital used Braden Q Pediatric Skin Risk Assessment Scale (Pediatric Braden Q Scale) and one hospital used Neonatal/Infant Braden Q scale (Neonatal/Infant Braden Q Scale). 129 nurses from 5 hospitals evaluated the scales routinely used by the department.Nearly 93.80% (121/129) of the nurses thought the scales were easy to understand, 76.74% (99/129) of the nurses thought the scales were easy to measure, 76.74% (99/129) of the nurses thought the scales were good at predicting, 75.19% (97/129) of the nurses thought the scales were suitable for newborns, 37.21% (48/129) of the nurses thought the scales did not adequately assess the severity of the condition, 34.11% (44/129) of the nurses thought the scales did not adequately assess the medical device factors, 24.81% (32/129) of the nurses thought the scales scores were not clearly defined. Conclusion The use of risk assessment scale for neonatal PI has not been unified yet, and the three scales need to be improved. The department should pay attention to the risk assessment of newborn PI and strengthen the awareness of PI prevention.