1.Effects of electromagnetic radiation on the nervous system in rats
Jianping ZHANG ; Shuanghong CHEN ; Guozhen GUO ; Hongyuan ZHOU ; Peng GAO ; Xiaomeng REN
Chinese journal of nautical medicine and hyperbaric medicine 2022;29(3):367-371
Objective:To study the effects of electromagnetic radiation(EMR)on the nervous system of rats in a simulated warship environment,so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups,with six rats in each group. There were four exposure groups:60 V/m exposure for 1 d and 3 d,120 V/m exposure for 1 d and 3 d. There were two control groups:control group for 1 d and control group for 3 d. After exposure,cholinergic neurotransmitters,blood-brain barrier permeability,and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group[(515.52±5.88)pmol/L]was lower than that in the control group for 3 d[(550.94±20.44)pmol/L],with statistically significant difference( P<0.05). The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group[(578.84±25.14)pmol/L]and the 120 V/m exposure for 1 d group[(519.62±13.09)pmol/L]were both lower than that in the control group for 1 d[(605.13±17.99)pmol/L],with statistically significant differences( P<0.05; P<0.01). The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group[(586.20±12.20)pmol/L]and the 120 V/m exposure for 3 d group[(591.22±9.78)pmol/L]were both lower than that in the control group for 3 d[(623.68±15.07)pmol/L],with statistically significant differences( P<0.01). The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups( P<0.01),respectively;the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups( P<0.01);after radio frequency(RF)exposure,the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m,the cholinergic neurotransmitters in the brains of SD rats have changed,the permeability of blood-brain barrier is increased,and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats,suggesting that the EMR of this band can bring potential harm to the deck crew,which demands appropriate protective measures.
2.Effects of electromagnetic radiation on the nervous system in rats
Jianping ZHANG ; Shuanghong CHEN ; Guozhen GUO ; Hongyuan ZHOU ; Peng GAO ; Xiaomeng REN
Chinese journal of nautical medicine and hyperbaric medicine 2022;29(3):367-371
Objective:To study the effects of electromagnetic radiation(EMR)on the nervous system of rats in a simulated warship environment,so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups,with six rats in each group. There were four exposure groups:60 V/m exposure for 1 d and 3 d,120 V/m exposure for 1 d and 3 d. There were two control groups:control group for 1 d and control group for 3 d. After exposure,cholinergic neurotransmitters,blood-brain barrier permeability,and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group[(515.52±5.88)pmol/L]was lower than that in the control group for 3 d[(550.94±20.44)pmol/L],with statistically significant difference( P<0.05). The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group[(578.84±25.14)pmol/L]and the 120 V/m exposure for 1 d group[(519.62±13.09)pmol/L]were both lower than that in the control group for 1 d[(605.13±17.99)pmol/L],with statistically significant differences( P<0.05; P<0.01). The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group[(586.20±12.20)pmol/L]and the 120 V/m exposure for 3 d group[(591.22±9.78)pmol/L]were both lower than that in the control group for 3 d[(623.68±15.07)pmol/L],with statistically significant differences( P<0.01). The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups( P<0.01),respectively;the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups( P<0.01);after radio frequency(RF)exposure,the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m,the cholinergic neurotransmitters in the brains of SD rats have changed,the permeability of blood-brain barrier is increased,and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats,suggesting that the EMR of this band can bring potential harm to the deck crew,which demands appropriate protective measures.
3.The anterograde sural neurovascular flap based on the popliteal artery perforator for covering tissue defects in middle and distal upper leg
Tingyu ZHOU ; Aixi YU ; You ZHANG ; Bin ZHONG ; Junsheng DU ; Jin CHEN ; Shuanghong GUO
Chinese Journal of Microsurgery 2016;39(5):428-431
Objective To evaluate the clinical effect of popliteal artery perforator-based sural neurovascular flap for repairing soft tissue defects in middle and distal upper leg.Methods Between December,2011 to September,2015,18 cases with skin soft tissue defects on the middle and distal upper leg were treated with popliteal artery perforator-based sural neurovascular flap,in which 12 cases were males,and 6 cases were females.The age was from 24 to 55 years,with the average age of 35.9 years.The size of tissue defects ranged from 3.0 cm × 3.0 cm to 7.0 cm × 9.0 cm.Results All flaps survived completely in 18 cases,1 case of marginal infection heated after dressing,and the rest cases in Ⅰ healing.The outline and function of survived flap were satisfactory during 3-16 months follow-up,with two-point discrimination of 4.0-10.0 mm.Conclusion The anterograde sural neurovascular flap based on the popliteal artery perforator provides a practical option for covering tissue defects in middle and distal upper leg.This flap is characteristiced by reliable blood supply without sacrificing main vesses,good contour and texture,in addition,the operation is easy of handling.

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