ObjectiveTo explore the effect of modified Ditan Decoction （涤痰汤） on chronic intermittent hypoxia cognitive function and the potential function mechanism. MethodsTwenty-four Sprague-Dawley （SD） rats were randomly divided into a normal group， a model group， and a modified Ditan Decoction group， with eight rats in each group. Rats in the modified Ditan Decoction group were administered the decoction by gavage at 14.8 ml/（kg·d）， while the normal group and the model group received the same dose of normal saline. Thirty minutes after daily gavage， the rats in all three groups were placed in an intermittent hypoxia chamber. The oxygen concentration for the model group and the modified Ditan Decoction group was adjusted daily for 8 hours using a computer program to establish the model， while the normal group was exposed to the same airflow rate of ambient air. The intervention was continued for 12 weeks to establish a chronic intermittent hypoxia rat model. The Y-maze test was used to evaluate spatial working memory in the rats. Resting-state functional magnetic resonance imaging （rs-fMRI） was performed to detect whole-brain regional homogeneity （ReHo） and seed-based functional connectivity （FC）. Brain regions showing significant differences in rs-fMRI were selected for further analysis. Immunofluorescence was used to detect β-amyloid （Aβ） deposition and the number of ionized calcium-binding adapter molecule 1 （IBA1）-positive microglial cells. Immunohistochemistry was employed to assess the expression of synaptophysin （SYP）， the excitatory synapse marker vesicular glutamate transporter 1 （Vglut1）， and the inhibitory synapse marker vesicular γ-aminobutyric acid transporter （VGAT）. ResultsCompared with the normal group， the model group showed a reduced spontaneous alternation rate in the Y-maze test. The smoothed Z-score standardized regional homogeneity （SzReHo） value in the left entorhinal cortex significantly increased， and the FC value from this seed point to the left basal forebrain significantly reduced. Additionally， the model group exhibited significantly higher Aβ fluorescence intensity and Iba1 positivity in the left entorhinal cortex， decreased expression of SYP， Vglut1， and VGAT， along with an increased Vglut1/VGAT ratio （P＜0.05 or P＜0.01）. Compared to the model group， the modified Ditan Decoction group demonstrated an increased spontaneous alternation rate， a significantly reduced SzReHo value in the left entorhinal cortex， and a significantly increased FC value from this region to the left basal forebrain. Furthermore， this group showed significantly lower Aβ fluorescence intensity and Iba1 positivity in the left entorhinal cortex， increased levels of SYP， Vglut1， and VGAT， and a decreased Vglut1/VGAT ratio （P＜0.05 or P＜0.01）. ConclusionModified Ditan Decoction can reconstruct the projection from the left basal forebrain to the entorhinal cortex in chronic intermittent hypoxia， thereby reducing Aβ aggregation and excessive microglial activation in the left entorhinal cortex. This process improves the excitation/inhibition imbalance caused by synaptic remodeling， ultimately enhancing cognitive function in rats of chronic intermittent hypoxia.

BackgroundPreventing and intervening in adolescent gaming disorder is of significant practical importance. Gaming motivation is strongly linked to gaming addiction and serves a key function in comprehending and addressing addictive gaming behaviors. However， the relationship between components of gaming motivation and symptoms of gaming disorder remains unclear. ObjectiveTo explore the relationship between components of gaming motivation and symptoms of gaming disorder among adolescents， so as to provide references for the prevention and intervention of gaming disorder in this population. MethodsFrom January to February 2024， a cluster sampling method was employed to select 1 414 adolescents from four middle schools in Sichuan Province and Chongqing Municipality as participants in the study. Online Game Motivation Scale （OGMS） and Gaming Disorder Scale for Adolescents （GADIS-A） were administered. Network analysis methods were utilized to investigate the relationships between components of gaming motivation and symptoms of gaming disorder. ResultsThe network edge weights revealed that achievement motivation was positively correlated with impaired game control ability， continued gaming despite negative consequences and the frequency of symptom occurrence （r=0.115， 0.050， 0.076， P<0.05）. Social motivation was negatively correlated with negative consequences （r=-0.054， P<0.05），while immersion motivation was positively correlated with continued gaming despite negative consequences （r=0.032， P<0.05）. Achievement motivation exhibited the highest strength centrality （1.157） among the three components of gaming motivation. ConclusionThe connections between components of gaming motivation and symptoms of gaming disorder exhibit distinct patterns， with each motivational component influencing gaming disorder through specific symptom pathway. Among these components， achievement motivation plays the most critical role in the interplay between gaming motivation and symptoms of gaming disorder. ［Funded by Chongqing Science and Health Joint Medical Science and Technology Innovation Projects General Projects （number， 2023MSXM133）］

【Objective】 To explore the intention of voluntary blood donation in students from a middle vocational school in Jinhua and its influencing factors. 【Methods】 A total of 400 students were enrolled from a middle vocational school in Jinhua by convenience sampling, and a self-designed questionnaire was issued to each student to investigate the cognition, intention and attitude of voluntary blood donation. The influencing factors of voluntary blood donation intention were analyzed by Logistic regression. 【Results】 A total of 394 valid questionnaires(98.50%) were collected. For 394 surveyed students, the average score of cognition of voluntary blood donation was (7.27±2.69) points, and the overall rate of intention to voluntary blood donation was 21.32%. Logistic regression analysis equation consisted of family support, better cognition, feeling happy about blood donation and worrying about blood donation, with risk ratios(OR) as 31.78, 188.69, 26.27 and 0.01, respectively(all P<0.05). 【Conclusion】 Students from a middle vocational school showed poor congnition and intention of voluntary blood donation.Family support for voluntary blood donation, better cognition of voluntary blood donation and happiness for blood donation were positive factors, and the fear of blood donation was a negative factor.It is necessary to strengthen the publicity of blood donation among students in middle vocational school to improve their congnition and intention to voluntary blood donation.

Objective:To study effects of multilevel nutritional support based on Nutrition Risk Screening (NRS 2002) on nutritional status and prognosis of patients with craniocerebral tumor surgery.Methods:A total of 40 patients undergoing craniocerebral tumor surgery who were admitted to The Third People's Hospital of Hubei Province from November 2018 to October 2019 were included as the control group. A total of 40 patients undergoing craniocerebral tumor surgery who were admitted to the Third People's Hospital of Hubei Province from November 2019 to November 2020 were included as the observation group. The control group was given routine nutritional support, and the observation group was given multi-level nutritional support based on NRS 2002. The nutritional status, serum nutritional indexes, prognosis and complications were compared between the two groups before the intervention and one month after the intervention.Results:After 1 month of intervention, the thickness of triceps skinfold and muscle circumference of unaffected upper arm in the two groups were greater than those before intervention, and those of the observation group were greater than the control group, and the differences were statistically significant ( P<0.05) . The levels of prealbumin, albumin and hemoglobin in the two groups were higher than those before the intervention, and the observation group were higher than the control group, and the differences were statistically significant ( P<0.05) . The Glasgow score and ability of daily living score of the two groups were higher than those before the intervention, and the observation group were higher than the control group, and the differences were statistically significant ( P<0.05) . After 1 month of intervention, there were no statistically significant differences in body mass index, constipation, diarrhea, pulmonary infection and malnutrition between the two groups ( P>0.05) . Conclusions:Multilevel nutritional support based on NRS 2002 can improve the nutritional status and prognosis of patients undergoing craniocerebral tumor surgery and the application effect is good.

Objective:To study the effects of electromagnetic radiation（EMR）on the nervous system of rats in a simulated warship environment，so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups，with six rats in each group. There were four exposure groups：60 V/m exposure for 1 d and 3 d，120 V/m exposure for 1 d and 3 d. There were two control groups：control group for 1 d and control group for 3 d. After exposure，cholinergic neurotransmitters，blood-brain barrier permeability，and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group［（515.52±5.88）pmol/L］was lower than that in the control group for 3 d［（550.94±20.44）pmol/L］，with statistically significant difference（ P<0.05）. The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group［（578.84±25.14）pmol/L］and the 120 V/m exposure for 1 d group［（519.62±13.09）pmol/L］were both lower than that in the control group for 1 d［（605.13±17.99）pmol/L］，with statistically significant differences（ P<0.05； P<0.01）. The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group［（586.20±12.20）pmol/L］and the 120 V/m exposure for 3 d group［（591.22±9.78）pmol/L］were both lower than that in the control group for 3 d［（623.68±15.07）pmol/L］，with statistically significant differences（ P<0.01）. The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups（ P<0.01），respectively；the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups（ P<0.01）；after radio frequency（RF）exposure，the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m，the cholinergic neurotransmitters in the brains of SD rats have changed，the permeability of blood-brain barrier is increased，and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats，suggesting that the EMR of this band can bring potential harm to the deck crew，which demands appropriate protective measures.

Objective:To study the effects of electromagnetic radiation（EMR）on the nervous system of rats in a simulated warship environment，so as to provide reference for improving the working condition for deck crew.Methods:A total of 36 adult male SD rats were randomly divided into six groups，with six rats in each group. There were four exposure groups：60 V/m exposure for 1 d and 3 d，120 V/m exposure for 1 d and 3 d. There were two control groups：control group for 1 d and control group for 3 d. After exposure，cholinergic neurotransmitters，blood-brain barrier permeability，and HSP70 were detected.Results:The content of ACh in hippocampus in the 60 V/m exposure for 3 d group［（515.52±5.88）pmol/L］was lower than that in the control group for 3 d［（550.94±20.44）pmol/L］，with statistically significant difference（ P<0.05）. The contents of ACh in cerebral cortex in the 60 V/m exposure for 1 d group［（578.84±25.14）pmol/L］and the 120 V/m exposure for 1 d group［（519.62±13.09）pmol/L］were both lower than that in the control group for 1 d［（605.13±17.99）pmol/L］，with statistically significant differences（ P<0.05； P<0.01）. The contents of ACh in cerebral cortex in 60 V/m exposure for 3 d group［（586.20±12.20）pmol/L］and the 120 V/m exposure for 3 d group［（591.22±9.78）pmol/L］were both lower than that in the control group for 3 d［（623.68±15.07）pmol/L］，with statistically significant differences（ P<0.01）. The contents of AChE in hippocampus and cerebral cortex were significantly increased in all four exposure groups compared with their correspondent control groups（ P<0.01），respectively；the concentrations of S100β in serum were significantly increased in the exposure for 3 d groups（ P<0.01）；after radio frequency（RF）exposure，the positive expressions of Hsp70 increased rapidly. Conclusion:After 1 day or 3 days of S-band RF exposure with electric field intensities of 60 V/m and 120 V/m，the cholinergic neurotransmitters in the brains of SD rats have changed，the permeability of blood-brain barrier is increased，and the positive expressions of Hsp70 are increased. It is speculated that S-band RF exposure can affect neurological system of SD rats，suggesting that the EMR of this band can bring potential harm to the deck crew，which demands appropriate protective measures.

Objective:To find an effective statistical analysis model for analyzing the detected concentration of the cabin airborne microorganism, so as to provide a method to obtain the theoretical limits of allowable concentration of cabin airborne microbial pollution.Methods:According to the principles of mathematical statistics, the detected data were processed by five mapping transformations: linear transformation y= x, negative exponential transformation y=1/ x, root exponential transformation y=sqrt( x), logarithmic transformation y=log10( x), and inverse trigonometric function transformation y=arctg( x). The distribution of data was analyzed by six probability function models: normal distribution, gamma distribution, exponential distribution, Rayleigh distribution, Weibull distribution, and beta distribution; when the mapping transformation confidence level α=0.05, Kolmogorov-Smirnov test (KS test) was used to test the acceptability of the model. When P≥α, the distribution model was deemed as "not to be rejected" . Results:After linear transformation, negative exponential transformation, root exponential transformation, and logarithmic transformation, the detected concentrations of the cabin airborne microorganism of large surface warships and submarines accepted normal distribution model, gamma distribution model, and Weibull distribution model ( P≥α), while that in the ordinary surface warships accepted Weibull distribution model ( P≥α). Conclusion:Weibull distribution model has good generality in the statistical analysis of the detected concentration of the cabin airborne microorganism, which can be used to calculate the theoretical parameters of allowable concentration.

Objective:To find an effective statistical analysis model for analyzing the detected concentration of the cabin airborne microorganism, so as to provide a method to obtain the theoretical limits of allowable concentration of cabin airborne microbial pollution.Methods:According to the principles of mathematical statistics, the detected data were processed by five mapping transformations: linear transformation y= x, negative exponential transformation y=1/ x, root exponential transformation y=sqrt( x), logarithmic transformation y=log10( x), and inverse trigonometric function transformation y=arctg( x). The distribution of data was analyzed by six probability function models: normal distribution, gamma distribution, exponential distribution, Rayleigh distribution, Weibull distribution, and beta distribution; when the mapping transformation confidence level α=0.05, Kolmogorov-Smirnov test (KS test) was used to test the acceptability of the model. When P≥α, the distribution model was deemed as "not to be rejected" . Results:After linear transformation, negative exponential transformation, root exponential transformation, and logarithmic transformation, the detected concentrations of the cabin airborne microorganism of large surface warships and submarines accepted normal distribution model, gamma distribution model, and Weibull distribution model ( P≥α), while that in the ordinary surface warships accepted Weibull distribution model ( P≥α). Conclusion:Weibull distribution model has good generality in the statistical analysis of the detected concentration of the cabin airborne microorganism, which can be used to calculate the theoretical parameters of allowable concentration.

Objective:To study the inactivation efficacy of pulsed light with different irradiation intensities on microorganisms, so as to provide a validation for the design of sterilization techniques.Methods:Using Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC9372) as experimental bacteria. The carrier quantitative germicidal test was used to evaluate the inactivation efficacy. The exponential dilution method and the pour plate method were used to measure the inactivation efficacy. The scanning electron microscope and transmission electron microscope were used to observe the effects of different irradiation intensities of light on bacterial structure. The bacterial control group (CO group) and the bacterial test group (T group) were set up with the same initial concentration in the experiment. In the CO group, the bacteria without pulsed light irradiation were cultured in parallel for 30 s, 60 s, and 90 s. In the T group, bacteria received irradiation 50 cm, 100 cm, 150 cm, and 200 cm away from the pulsed light source respectively, and the irradiation time duration was set as 30 s, 60 s, and 90 s for each distance.Results:The inactivation rate of ATCC25923 was 100% and that of ATCC9372 was 99.9% when they were 50 cm and 100 cm away from the pulsed light source for 30 s. When the irradiation distance was increased to 150 cm, the inactivation rates of ATCC25923 for 30 s, 60 s, and 90 s were 81.9%, 95.4%, and 100%, respectively; and those of ATCC9372 were 76.0%, 92.3%, and 99.9%, respectively. When the irradiation distance was increased to 200 cm, the inactivation efficiency of pulsed light was significantly reduced; the inactivation rates of ATCC25923 at the three detection time points were reduced to 24.5%, 38.8%, and 48.8%, respectively, and those of ATCC9372 were reduced to 14.2%, 20.0%, and 28.5%.Conclusion:Pulsed light can quickly eradicate microorganisms, and its inactivation rate and efficiency are closely related to the intensity of irradiation.

Objective:To study the inactivation efficacy of pulsed light with different irradiation intensities on microorganisms, so as to provide a validation for the design of sterilization techniques.Methods:Using Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC9372) as experimental bacteria. The carrier quantitative germicidal test was used to evaluate the inactivation efficacy. The exponential dilution method and the pour plate method were used to measure the inactivation efficacy. The scanning electron microscope and transmission electron microscope were used to observe the effects of different irradiation intensities of light on bacterial structure. The bacterial control group (CO group) and the bacterial test group (T group) were set up with the same initial concentration in the experiment. In the CO group, the bacteria without pulsed light irradiation were cultured in parallel for 30 s, 60 s, and 90 s. In the T group, bacteria received irradiation 50 cm, 100 cm, 150 cm, and 200 cm away from the pulsed light source respectively, and the irradiation time duration was set as 30 s, 60 s, and 90 s for each distance.Results:The inactivation rate of ATCC25923 was 100% and that of ATCC9372 was 99.9% when they were 50 cm and 100 cm away from the pulsed light source for 30 s. When the irradiation distance was increased to 150 cm, the inactivation rates of ATCC25923 for 30 s, 60 s, and 90 s were 81.9%, 95.4%, and 100%, respectively; and those of ATCC9372 were 76.0%, 92.3%, and 99.9%, respectively. When the irradiation distance was increased to 200 cm, the inactivation efficiency of pulsed light was significantly reduced; the inactivation rates of ATCC25923 at the three detection time points were reduced to 24.5%, 38.8%, and 48.8%, respectively, and those of ATCC9372 were reduced to 14.2%, 20.0%, and 28.5%.Conclusion:Pulsed light can quickly eradicate microorganisms, and its inactivation rate and efficiency are closely related to the intensity of irradiation.