Ruxolitinib， a small molecule inhibitor， selectively targets Janus kinase （JAK） by competitively binding to adenosine triphosphate on the catalytic site of the JAK1 and JAK2 domain， thereby inhibiting JAK activation and signal transducer and activator of transcription （STAT） phosphorylation and prevents the expressions of the JAK-STAT signaling pathway. Oral ruxolitinib has demonstrated promising efficacy for myelofibrosis and polycythemia vera. The topical Ruxolitinib cream， approved by the US FDA as the first non-segmental vitiligo home treatment drug， is set to be launched in domestic medical pioneer areas in August 2023 and is expected to bring about a breakthrough in the treatment of vitiligo. Clinical cases have also shown that Ruxolitinib cream has significant curative effects on atopic dermatitis， alopecia areata， and other conditions， indicating great application prospects.

Objective:To investigate the clinical effect of drugs combined with hyperbaric oxygen（HBO）on nervous system damage caused by nitrous oxide（N 2O）poisoning. Methods:The clinical data of 12 patients with nerve damage caused by N 2O poisoning admitted to the Department of Neurology，Hyperbaric Oxygen of a certain PLA hospital from February 2020 to April 2021 were reviewed. The changes of neuroelectrophysiological and neurological functions of patients before and after treatment were analyzed using electroneurogram detection，Berg balance scale，and Holden’s functional ambulation categories（FAC）as observation indicators. Results:After drug treatment combined with HBO，the motor conduction velocity，sensory conduction velocity，motor latency，motor evoked potential，and sensory evoked potential of electroneurogram of 12 patients were significantly improved，and the differences were statistically significant（ P < 0.05）.Berg balance scale scores were significantly improved compared with those before treatment，and the differences were statistically significant（ P < 0.05）.FAC scale was improved compared with before treatment，but the difference was not statistically significant（ P > 0.05）. Conclusion:HBO combined with folic acid，vitamin B12 and other neurotrophic drugs has a certain clinical effect in the treatment of nerve damage caused by N 2O poisoning，which is worthy of further study and improvement.

Objective:To investigate the clinical effect of drugs combined with hyperbaric oxygen（HBO）on nervous system damage caused by nitrous oxide（N 2O）poisoning. Methods:The clinical data of 12 patients with nerve damage caused by N 2O poisoning admitted to the Department of Neurology，Hyperbaric Oxygen of a certain PLA hospital from February 2020 to April 2021 were reviewed. The changes of neuroelectrophysiological and neurological functions of patients before and after treatment were analyzed using electroneurogram detection，Berg balance scale，and Holden’s functional ambulation categories（FAC）as observation indicators. Results:After drug treatment combined with HBO，the motor conduction velocity，sensory conduction velocity，motor latency，motor evoked potential，and sensory evoked potential of electroneurogram of 12 patients were significantly improved，and the differences were statistically significant（ P < 0.05）.Berg balance scale scores were significantly improved compared with those before treatment，and the differences were statistically significant（ P < 0.05）.FAC scale was improved compared with before treatment，but the difference was not statistically significant（ P > 0.05）. Conclusion:HBO combined with folic acid，vitamin B12 and other neurotrophic drugs has a certain clinical effect in the treatment of nerve damage caused by N 2O poisoning，which is worthy of further study and improvement.

Objective:To explore efficacy and safety of endovascular therapy combined with hyperbaric oxygen (HBO) intervention in acute anterior circulation macrovascular occlusion stroke.Methods:A total of 150 patients with acute anterior circulation macrovascular occlusive stroke admitted in No.902 Hospital of the CPLA Joint Logistic Support Force were selected and randomly divided into endovascular treatment group and combined treatment group (endovascular+ HBO). Endovascular therapy was adopted in the treatment of all patients. Modified thrombolytic flow grading (mTICI) was used to evaluate vascular recanalization after operation, and the incidences of complications of hemorrhage transformation and vascular re-occlusion were recorded. National institutes of health stroke scale (NIHSS) was used to evaluate the neurological functions on admission, at 24 hours after the operation, and one course after HBO therapy. The prognosis was evaluated by observing the changes of blood homocysteine (HCY) and high-sensitivity C-reactive protein (hs-CRP) levels before and after treatment. The patients had been followed up by the modified Rankin Scale (mRS) for 90 days. At the same time, 75 patients with acute anterior circulation macrovascular occlusive stroke without taking endovascular treatment were selected as the control group.Results:Among 150 patients, 126 cases had a grade of mTICI≥2b, while 24 cases had a grade of mTICI<2b. Postoperative vascular re-occlusion occurred in 3 cases, and symptomatic intracranial hematoma occurred in 6 cases. The NIHSS score at 24 hours after the operation was significantly lower than that on admission, and the NIHSS score was further improved after HBO treatment. Ninety days after the operation, mRS showed 98 cases of good prognoses, while 52 cases of poor prognoses. The levels of HCY and hs-CRP were significantly lower than those before the treatment.Conclusion:Endovascular therapy for acute anterior circulation macrovascular occlusive stroke is safe and effective. Postoperative HBO therapy can significantly improve the prognosis and the quality of life of patients.

Objective:To explore efficacy and safety of endovascular therapy combined with hyperbaric oxygen (HBO) intervention in acute anterior circulation macrovascular occlusion stroke.Methods:A total of 150 patients with acute anterior circulation macrovascular occlusive stroke admitted in No.902 Hospital of the CPLA Joint Logistic Support Force were selected and randomly divided into endovascular treatment group and combined treatment group (endovascular+ HBO). Endovascular therapy was adopted in the treatment of all patients. Modified thrombolytic flow grading (mTICI) was used to evaluate vascular recanalization after operation, and the incidences of complications of hemorrhage transformation and vascular re-occlusion were recorded. National institutes of health stroke scale (NIHSS) was used to evaluate the neurological functions on admission, at 24 hours after the operation, and one course after HBO therapy. The prognosis was evaluated by observing the changes of blood homocysteine (HCY) and high-sensitivity C-reactive protein (hs-CRP) levels before and after treatment. The patients had been followed up by the modified Rankin Scale (mRS) for 90 days. At the same time, 75 patients with acute anterior circulation macrovascular occlusive stroke without taking endovascular treatment were selected as the control group.Results:Among 150 patients, 126 cases had a grade of mTICI≥2b, while 24 cases had a grade of mTICI<2b. Postoperative vascular re-occlusion occurred in 3 cases, and symptomatic intracranial hematoma occurred in 6 cases. The NIHSS score at 24 hours after the operation was significantly lower than that on admission, and the NIHSS score was further improved after HBO treatment. Ninety days after the operation, mRS showed 98 cases of good prognoses, while 52 cases of poor prognoses. The levels of HCY and hs-CRP were significantly lower than those before the treatment.Conclusion:Endovascular therapy for acute anterior circulation macrovascular occlusive stroke is safe and effective. Postoperative HBO therapy can significantly improve the prognosis and the quality of life of patients.

Objective To evaluate the effect of TanshinonneⅡA preconditioning on nuclear factor kappa B ( NF-κB ) signaling pathway in N9 cells subjected to oxygen-glucose deprivation and restoration ( OGD∕R) . Methods N9 cells cultured in vitro were divided into 4 groups ( n=16 each) by using a ran-dom number table method: control group ( C group) , OGD∕R group, vehicle group ( V group) and Tanshi-none ⅡA preconditioning group ( TP group) . N9 cells were subjected to O2-glucose deprivation for 4 h fol-lowed by restoration of O2-glucose supply for 24 h to establish the OGD∕R model. Group C receive no treat-ment. In group TP, N9 cells were cultured in medium containing Tanshinone ⅡA ( final concentration 5 mg∕L) for 24 h before the model was established. Dimethyl sulfoxide was added to the culture medium in group V. At the 24 h of reoxygenation, the level of lactate dehydrogenase ( LDH) and nitric oxide ( NO) in culture medium was detected by spectrophotometry, cell apoptosis was assessed by using TUNEL, and the expression of NF-κB in nucleus was detected by Western blot. The apoptosis rate was calculated. Results Compared with group C, the levels of LDH, IL-β, TNF-α and NO in culture medium and apoptosis rate were significantly increased, and the expression of NF-κB in nucleus was up-regulated in OGD∕R, V and TP groups ( P<0. 05) . Compared with group OGD∕R, the levels of LDH, IL-β, TNF-αand NO in culture me-dium and apoptosis rate were significantly decreased, and the expression of NF-κB in nucleus was down-reg-ulated in group TP ( P<0. 05) , and no significant change was found in the parameters mentioned above in group V ( P>0. 05 ) . Conclusion The mechanism by which Tanshinonne ⅡA preconditioning reduces OGD∕R injury to N9 cells is related to inhibiting NF-κB signaling pathway.

Objective To investigate the effects of multi-mode revascularization combined with hyperbaric oxygen (HBO) in the treatment of acute internal carotid artery occlusion.Methods One mid-aged female patient was admitted into hospital 1 hour after sudden onset of unconsciousness.Past medical history included rheumatic heart disease and atrial fibrillation.Digital subtraction angiography (DSA) showed occlusion at the distal end of internal carotid artery.With the knowledge and consent of the dependants,endovascular intervention treatment was performed and HBO therapy was supplemented after surgery.Results Due to the location,size and complexity of shapes of thrombi,re-canalization was not successful after trying in sequence stent thrombectomy,balloon catheter disruption of thrombi and contact arterial thrombolysis.Finally,the occluded vessel was re-canalized successfully by the catheter aspiration technique,and the prognosis of the patient improved significantly.Conclusions This case suggests that the combined use of multi-mode endovascular interventional technology is an effective method for re-canalizing occluded vessels.Supplemented HBO therapy after surgery is beneficial to the improvement of prognosis.

Objective To investigate the impact of carotid artery stenting (CAS) combined with hyperbaric oxygen (HBO) on cerebral hemodynamics in patients with internal carotid artery stenosis.Methods Seventy-eight patients with severe unilateral carotid artery stenosis were randomly divided into the control group (n =36) and the experimental group (n =42),which all received carotid artery stenting.After surgery,the control group was treated with anti-platelet and other routine treatment,while the experimental group received HBO therapy within 24 hours after surgery,in addition to routine treatment.Trancranial Doppler (TCD) and Computed tomography perfusion (CTP) were used to detect changes in cerebral hemodynamics both before and after surgery,as well as 2 weeks after surgery,and the rate of stroke recurrence was also observed within one year of surgery.Results Mean velocity (Vm) of blood flow,peak systolic velocity (PSV),pulse index (PI) and cerebrovascular reserve (CVR) of the affected middle cerebral artery were significantly increased after surgery.Moreover,2 weeks after surgery,regional cerebral blood volume and flow (rCBV and rCBF) of the experimental group were also significantly higher,as compared with those before surgery (P ＜ 0.05).Mean transit time (rMTT) and peak time (rTTP) were all lower than those before surgery.Changes in rCBF,rMTT and rTTP 2 weeks after surgery were all greater than those of the control group(P ＜ 0.05).Oneyear follow-up indicated that stroke recurrence rate of the experimental group was lower than that of the control group (11.9％ vs.16.7％,P ＜ 0.05).Conclusions Carotid artery stenting combined with HBO intervention could improve cerebral hemodynamics in patients with severe internal carotid artery stenosis,and at the same time could reduce stroke recurrence rate.

Objective To investigate the impact of carotid artery stenting (CAS) combined with hyperbaric oxygen (HBO) on cerebral hemodynamics in patients with internal carotid artery stenosis.Methods Seventy-eight patients with severe unilateral carotid artery stenosis were randomly divided into the control group (n =36) and the experimental group (n =42),which all received carotid artery stenting.After surgery,the control group was treated with anti-platelet and other routine treatment,while the experimental group received HBO therapy within 24 hours after surgery,in addition to routine treatment.Trancranial Doppler (TCD) and Computed tomography perfusion (CTP) were used to detect changes in cerebral hemodynamics both before and after surgery,as well as 2 weeks after surgery,and the rate of stroke recurrence was also observed within one year of surgery.Results Mean velocity (Vm) of blood flow,peak systolic velocity (PSV),pulse index (PI) and cerebrovascular reserve (CVR) of the affected middle cerebral artery were significantly increased after surgery.Moreover,2 weeks after surgery,regional cerebral blood volume and flow (rCBV and rCBF) of the experimental group were also significantly higher,as compared with those before surgery (P ＜ 0.05).Mean transit time (rMTT) and peak time (rTTP) were all lower than those before surgery.Changes in rCBF,rMTT and rTTP 2 weeks after surgery were all greater than those of the control group(P ＜ 0.05).Oneyear follow-up indicated that stroke recurrence rate of the experimental group was lower than that of the control group (11.9％ vs.16.7％,P ＜ 0.05).Conclusions Carotid artery stenting combined with HBO intervention could improve cerebral hemodynamics in patients with severe internal carotid artery stenosis,and at the same time could reduce stroke recurrence rate.

Objective To investigate the effects of multi-mode revascularization combined with hyperbaric oxygen (HBO) in the treatment of acute internal carotid artery occlusion.Methods One mid-aged female patient was admitted into hospital 1 hour after sudden onset of unconsciousness.Past medical history included rheumatic heart disease and atrial fibrillation.Digital subtraction angiography (DSA) showed occlusion at the distal end of internal carotid artery.With the knowledge and consent of the dependants,endovascular intervention treatment was performed and HBO therapy was supplemented after surgery.Results Due to the location,size and complexity of shapes of thrombi,re-canalization was not successful after trying in sequence stent thrombectomy,balloon catheter disruption of thrombi and contact arterial thrombolysis.Finally,the occluded vessel was re-canalized successfully by the catheter aspiration technique,and the prognosis of the patient improved significantly.Conclusions This case suggests that the combined use of multi-mode endovascular interventional technology is an effective method for re-canalizing occluded vessels.Supplemented HBO therapy after surgery is beneficial to the improvement of prognosis.