Objective To observe the clinical efficacy of Tongdu Tiaoyang rolling needle pricking-cupping(RNP-C)therapy for cervical spondylosis of qi stagnation and blood stasis type.Methods A total of 90 patients with cervical spondylosis of qi stagnation and blood stasis type were randomly divided into the rolling needle pricking-cupping(RNP-C)group and the electroacupuncture(EA)group,with 45 cases in either group.Both groups were treated with electroacupuncture at Tianzhu(BL 10),Jingjiaji(EX-B2),Houxi(SI 3),Shenmai(BL 62),Xuanzhong(GB 39),Geshu(BL 17),Hegu(LI 4)and Ashi points with continuous wave and 2 Hz of frequency for once a day,7 times as a treatment course.On the basis of electroacupuncture treatment,the rolling needle pricking-cupping(RNP-C)group was treated with bloodletting by rolling needle on the neck Du Mai and three yang channels,from Fengfu(DU 16)to Taodao(DU 13),Yuzhen(BL 9)to Dazhu(BL 11),Fengchi(GB 20)to Jianjing(GB 21),and Yifeng(SJ 17)to Tianyou(SJ 16),followed by fire cupping,for once every other day,and 3 times in all.The visual analogue scale(VAS)scores and Japanese Orthopaedic Association Scores(JOA)were observed before and after treatment,and the efficacy was evaluated.Results Compared before treatment,the scores of VAS in either group were all significantly reduced after treatment(P＜0.01).The scores of VAS in the RNP-C group were significantly inferior to those in the EA group after treatment(P＜0.01).Compared before treatment,the scores of JOA in either group were all significantly promoted after treatment(P＜0.01).The differences of JOA scores between the RNP-C group and EA group after treatment were not significant(P＞0.05).The therapeutic effecacy of the RNP-C group is superior to that of the traditional EA group(P＜0.05).Conclusion Tongdu Tiaoyang rolling needle pricking-cupping(RNP-C)therapy combining with traditional electroacupuncture could improve the cervical pain symptoms of patients with cervical spondylosis of qi stagnation and blood stasis type in a short period of time,and the clinical efficacy is better than traditional electroacupuncture.

Objective To evaluate the clinical efficacy of electroacupuncture(EA)in the treatment of mild to moderate depression with the method of"Regulating the Governor Vessel to Relieve Depression".Methods In a randomized,single-blind,parallel-controlled design,64 patients with depression were enrolled and assigned to the EA group and the control group in a 1∶1 ratio.In the EA group,Baihui,Shenting,Yintang,Neiguan,Sanyinjiao,Hegu and Taichong were selected,electrodes were connected to Baihui and Yintang,continuous wave and frequency of 2 Hz were applied,and each session was given for 30 min,3 times a week for 8 weeks.The control group used Streitberger needles which couldn't be inserted into the skin,and the acupoints and treatment course were the same as the EA group.The primary outcome was the 17-item Hamilton Depression Rating Scale(HAMD-17);secondary outcomes included the Patient Health Questionnaire(PHQ-9),the Self-rating Anxiety Scale(SAS)and the 36-item Short-form Health Survey(SF-36).Patients were assessed before treatment,at weeks 4 and 8 after treatment,and at the follow-up visit at week 12.Results 31 cases in the EA group finished the trial and 30 in the control group,with an overall dropout rate of 4.7％.Using the intention-to-treat analysis,patients'HAMD-17 scores in the EA group were lower than those in the control group at weeks 4,8 and 12 of treatment(P＜0.01).HAMD-17 scores decreased over time in both groups,but the EA's downward trend was more pronounced.At week 8,compared with the control group,the PHQ-9 and SAS scores were lower in the EA group(P＜0.01),the EA group scored higher on all 3 dimensions of SF-36,energy,social functioning and mental health(P＜0.01).Conclusion Compared with sham acupuncture,the EA treatment was more effective in improving depression,relieving anxiety and improving the quality of life of patients with mild to moderate depression.

Insomnia disorder is a common disease in acupuncture clinics.There is a tendency for the theory to solidify and lag behind the clinic.In this paper,we examined insomnia disorder from the perspective of acupuncture and moxibustion theory.Based on the general law of acupuncture and moxibustion to recognize the disease,we concerned that the basic understanding of the disease has commonality between ancient and modern medical practitioners,and the theory can be deconstructed as follows:the theory of"yin and yang"is the starting point and the root of the theory,the theory of"spirit regulars the sleep"is the directly related theory,the theory of internal organs is the basis of the disease mechanism,the theory of meridians and acupoints is the key to diagnosis and treatment,and the theory of acupuncture and moxibustion is the key to treatment.The improvement and development of the theory of acupuncture for insomnia disorder can be carried out on this basis.

Objective To observe the clinical efficacy of Tongdu Tiaoyang rolling needle pricking-cupping(RNP-C)therapy for cervical spondylosis of qi stagnation and blood stasis type.Methods A total of 90 patients with cervical spondylosis of qi stagnation and blood stasis type were randomly divided into the rolling needle pricking-cupping(RNP-C)group and the electroacupuncture(EA)group,with 45 cases in either group.Both groups were treated with electroacupuncture at Tianzhu(BL 10),Jingjiaji(EX-B2),Houxi(SI 3),Shenmai(BL 62),Xuanzhong(GB 39),Geshu(BL 17),Hegu(LI 4)and Ashi points with continuous wave and 2 Hz of frequency for once a day,7 times as a treatment course.On the basis of electroacupuncture treatment,the rolling needle pricking-cupping(RNP-C)group was treated with bloodletting by rolling needle on the neck Du Mai and three yang channels,from Fengfu(DU 16)to Taodao(DU 13),Yuzhen(BL 9)to Dazhu(BL 11),Fengchi(GB 20)to Jianjing(GB 21),and Yifeng(SJ 17)to Tianyou(SJ 16),followed by fire cupping,for once every other day,and 3 times in all.The visual analogue scale(VAS)scores and Japanese Orthopaedic Association Scores(JOA)were observed before and after treatment,and the efficacy was evaluated.Results Compared before treatment,the scores of VAS in either group were all significantly reduced after treatment(P＜0.01).The scores of VAS in the RNP-C group were significantly inferior to those in the EA group after treatment(P＜0.01).Compared before treatment,the scores of JOA in either group were all significantly promoted after treatment(P＜0.01).The differences of JOA scores between the RNP-C group and EA group after treatment were not significant(P＞0.05).The therapeutic effecacy of the RNP-C group is superior to that of the traditional EA group(P＜0.05).Conclusion Tongdu Tiaoyang rolling needle pricking-cupping(RNP-C)therapy combining with traditional electroacupuncture could improve the cervical pain symptoms of patients with cervical spondylosis of qi stagnation and blood stasis type in a short period of time,and the clinical efficacy is better than traditional electroacupuncture.

Objective To evaluate the clinical efficacy of electroacupuncture(EA)in the treatment of mild to moderate depression with the method of"Regulating the Governor Vessel to Relieve Depression".Methods In a randomized,single-blind,parallel-controlled design,64 patients with depression were enrolled and assigned to the EA group and the control group in a 1∶1 ratio.In the EA group,Baihui,Shenting,Yintang,Neiguan,Sanyinjiao,Hegu and Taichong were selected,electrodes were connected to Baihui and Yintang,continuous wave and frequency of 2 Hz were applied,and each session was given for 30 min,3 times a week for 8 weeks.The control group used Streitberger needles which couldn't be inserted into the skin,and the acupoints and treatment course were the same as the EA group.The primary outcome was the 17-item Hamilton Depression Rating Scale(HAMD-17);secondary outcomes included the Patient Health Questionnaire(PHQ-9),the Self-rating Anxiety Scale(SAS)and the 36-item Short-form Health Survey(SF-36).Patients were assessed before treatment,at weeks 4 and 8 after treatment,and at the follow-up visit at week 12.Results 31 cases in the EA group finished the trial and 30 in the control group,with an overall dropout rate of 4.7％.Using the intention-to-treat analysis,patients'HAMD-17 scores in the EA group were lower than those in the control group at weeks 4,8 and 12 of treatment(P＜0.01).HAMD-17 scores decreased over time in both groups,but the EA's downward trend was more pronounced.At week 8,compared with the control group,the PHQ-9 and SAS scores were lower in the EA group(P＜0.01),the EA group scored higher on all 3 dimensions of SF-36,energy,social functioning and mental health(P＜0.01).Conclusion Compared with sham acupuncture,the EA treatment was more effective in improving depression,relieving anxiety and improving the quality of life of patients with mild to moderate depression.

Insomnia disorder is a common disease in acupuncture clinics.There is a tendency for the theory to solidify and lag behind the clinic.In this paper,we examined insomnia disorder from the perspective of acupuncture and moxibustion theory.Based on the general law of acupuncture and moxibustion to recognize the disease,we concerned that the basic understanding of the disease has commonality between ancient and modern medical practitioners,and the theory can be deconstructed as follows:the theory of"yin and yang"is the starting point and the root of the theory,the theory of"spirit regulars the sleep"is the directly related theory,the theory of internal organs is the basis of the disease mechanism,the theory of meridians and acupoints is the key to diagnosis and treatment,and the theory of acupuncture and moxibustion is the key to treatment.The improvement and development of the theory of acupuncture for insomnia disorder can be carried out on this basis.

Objective:To investigate the effects of airway humidification therapy on pertussis in infants.Methods:Sixty children with pertussis who received treatment in Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from May 2020 to September 2021 were included in this study. They were randomly divided into study and control groups, with 30 infants per group. The control group was treated with conventional drugs. The study group was subjected to airway humidification therapy based on conventional drug treatment. Clinical efficacy was evaluated in each group. Time to an improvement in clinical symptoms and length of hospital stay were recorded. Lung function indicators and serum inflammatory factor levels were determined before and after treatment. Pertussis-related complications were recorded.Results:The overall response rate in the study group was significantly higher than that in the control group (100.00% vs. 76.7%, χ2 = 5.82, P = 0.016). Time to disappearance of spastic cough, time to resolution of cyanosis, time to disappearance of pulmonary rales, and length of hospital stay in the study group were (3.10 ± 0.67) days, (1.53 ± 0.68) days, (4.27 ± 0.58) days, and (11.57 ± 0.73) days, respectively, which were significantly shorter than (4.23 ± 1.99) days, (2.67 ± 1.77) days, (5.63 ± 2.38) days, (13.33 ± 2.40) days ( t = 2.93, 3.27, 3.04, 3.86; P = 0.005, 0.002, 0.003, P < 0.001). Respiratory rate in the study group was significantly lower than that in the control group [(21.83 ± 1.15) breaths/minute vs. (24.23 ± 3.12) breaths/minute, t = 3.94, P = 0.002]. Tidal volume/kg body weight, the ratio of time to peak expiratory flow to total expiratory time and the ratio of volume to peak expiratory flow to total expiratory volume in the study group were (10.70 ± 0.81) mL/kg, (41.60 ± 1.57)%, (42.89 ± 1.44)%, respectively, which were significantly higher than (9.78 ± 1.34) mL/kg, (39.18 ± 3.37)%, (40.20 ± 3.05)% in the control group ( t = 3.21, 3.56, 4.36; P < 0.001, P = 0.001, P < 0.001). Serum tumor necrosis alpha (TNF-α), interleukin-6 and interleukin-8 levels in the study group were (62.44 ± 2.96) ng/L, (46.59 ± 1.96) ng/L, (54.63 ± 3.27) ng/L, respectively, which were significantly lower than (79.86 ± 3.30) ng/L, (58.20 ± 2.10) ng/L, (63.31 ± 3.86) ng/L in the control group ( t =21.53, 22.13, 9.38, all P < 0.001). The overall incidence of pertussis in the study group was significantly lower than that in the control group (3.3% vs. 26.7%, χ2 = 4.71, P = 0.030). Conclusion:Airway humidification therapy can shorten the time to reduction of symptoms of spasmodic pertussis, improve clinical efficacy, strengthen pulmonary ventilation function, lower respiratory tract inflammatory responses and reduce the risk of pertussis-related complications.

Objective To investigate the clinical,laboratory and imaging characteristics of children with Mycoplasma pneumoniae pneumonia (MPP) in different age groups.Methods One hundred and sixty-four children whose ages were 1 month to 14 years old with MPP hospitalized at Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine in 2017 were enrolled as study objects.All children were classified into 2 groups:1 month to 3 year-old group (46 cases) and ＞ 3 to 14 year-old group (118 cases).The clinical features,main laboratory and imaging findings of 2 groups were retrospectively analyzed and compared.Results (1) Compared with 1 month to 3 year-old group,＞ 3 to 14 year-old group had more patients with fever [97.5％ (115/118 cases) vs.84.8％ (39/46 cases)],longer duration of fever [48.3％ (57/118 cases) vs.23.9％ (11/46 cases)] and higher fever [83.1％ (98/ 118 cases) vs.52.2％ (24/46 cases)],but less patients with wheezing [2.5％ (3/118 cases) vs.43.5％ (20/46 cases)],shortness of breath and dyspnea [0.8％ (1/118 cases) vs.15.2％ (7/46 cases)],dry rale [1.7％ (2/118 cases) vs.60.9％ (28/46 cases)] and wet pulmonary rale [50.0％ (59/118 cases) vs.69.6％ (32/46 cases)],and the differences were statistically significant (all P ＜ 0.05).(2) The increase of platelet count,creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) in 1 month to 3 year-old group of MPP [316.0 (229.3,372.3) × 109/L,25.70 (17.70,31.98) U/L,346.5 (310.3,388.3) U/L] were more common than those in ＞ 3 to 14 year-old group [266.0 (205.8,317.8) × 109/L,21.50 (15.75,28.00) U/L,303.0 (173.0,352.0) U/L],while the increase of C reactive protein (CRP) and erythrocyte sedimentation rate (ESR) in ＞ 3 to 14 year-old group [12.66(9.16,19.44) mg/L,23.00(17.75,29.00) mm/1 h] were more common than those in 1 month to 3 year-old group[2.46 (0.54,11.63) mg/L,14.00 (10.00,20.25) mm/1 h],and there were significant statistical differences (all P ＜ 0.05).(3) The MPP imaging features of children in different age groups were diverse.In 1 month to 3 year-old group,the double lung involvement [80.4％ (37/46 cases)] was more obvious than that in ＞3 to 14 year-old group [55.9％ (66/118 cases)],and the difference was statistically significant (P ＜ 0.05).The lesions of 1 month to 3 year-old group were more common in lobular pulmonary [82.6％ (38/46 cases)],while in ＞ 3 to 14 year-old group,the radiographic findings were predominant with consolidation in the segment or lobe of the lung [33.1％ (39/118 cases)],and the difference was statistically significant (P ＜ 0.01).Conclusions Younger than 3 year-old children with MPP are prone to wheezing,shortness of breath,dyspnea and lung rale,while the rise of CK-MB and LDH is common.Imaging is mainly involved in double lung involvement and pulmonary lobule infiltration.The older the children with MPP are,the longer the fever duration is,and more common is the higher fever.The older the children,the more more easily they have the relative increase of CRP and ESR,and at the same time they are more likely to have the consolidation of the lung segment or lobe.

Objective: To observe the effects of electroacupuncture (EA) on depressive-like behavior, synaptic plasticity and 5-HTT protein expression of hippocampal CA1 region in the WKY model rats. Methods In this experiment, 10 male Wistar rats were selected as the normal group (Control), and 30 male WKY rats were randomly divided into model group, electroacupuncture group (EA) and sham needle group (Sham EA) . Behavioral testing was performed by the Sour Water Preference Experiment (SPT), the Open Field Experiment (OFT), and the Forced Swimming Experiment (FST) . The electrophysiological field potential experiment was used to detect the effect of electroacupuncture on LTP in the hippocampal CA1 region of rats with depression, which is the effect on synaptic plasticity. The expression of 5-HTT protein in hippocampal CA1 region was detected by Western blot. Results In the SPT, compared to the Control group, the sucrose solution intake was significantly reduced in the Model group (P ＜ 0.01) . It was improved after EA treatment compared to Sham EA group (P ＜ 0.05) . In the OFT, compared with the Control group, the center time, total move time, center distance, total distance, rearing and grooming incidents were all decreased in the Model group. Compared with the Model group, the center time and total move time in the EA group increased significantly after 3 weeks of treatment (P ＜0.05) . In the FST, duration of immobility was significantly (P ＜ 0.05) longer in the Model group comparing with the Control group. Compared with Sham EA group and Model group, the immobile time of EA group was significantly shorter (P ＜ 0.05) . Compared with the Control group, the fEPSP slope of the Model group was significantly shorter (P ＜ 0.001) .Compared with the Sham EA group, the fEPSP slope of the EA group increased significantly (P ＜ 0.001) . And the LTP of Sham EA and Model groups induced failure in hippocampal CA1 region, the LTP induction was successful after EA treatment. Compared with the Control group, the expression of 5-HTT protein was significantly increased (P＜0.001) .Compared with Sham EA group, the expression of 5-HTT protein in EA group decreased (P ＜ 0.001) . Conclusion EA could alleviate depression-like behaviors in depression model rats and reverse the impairment of hippocampus synaptic plasticity by decrease the over-expression of 5-HTT protein in hippocampal CA1 region. It may be one of the mechanisms of EA on depression.

Objective To observe the clinical efficacy of surround electroacupuncture in relieving pain after operation for mixed hemorrhoids. Method Seventy-two patients with mixed hemorrhoids were randomized into a treatment group and a control group, 36 cases in each group. The two groups both received the corresponding interventions once in 15 min after the operation: the treatment group received electroacupuncture treatment, and the control group was only placed in a simulated environment of electroacupuncture but without receivimg any needling treatment. The pain intensity was evaluated by using Verbal Rating Scale (VRS), Wong-Baker Faces Pain Rating Scale, and Visual Analogue Scale (VAS) at 11 different time points after the treatment: once every 1 h in 8 h after the treatment, once at the first defecation, once respectively 24 h and 48 h after the treatment. The data were statistically analyzed afterwards. Result At 3 h and 4 h after the treatment and at the first defecation after the treatment, the VRS scores of the treatment group were significantly lower than that of the control group (P<0.05); respectively at 4 h, 5 h, 6 h, 7 h, and 8 h after the treatment and at the first defecation, the Wong-Baker scores of the treatment group were significantly lower than that of the control group (P<0.05); respectively at 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, and 8 h after the treatment, at the first defecation, as well as at 24 h after the treatment, the VAS scores of the treatment group were significantly lower than that of the control group (P<0.05); it indicates that the pain intensity of the treatment group should be lower than that of the control group at the above time points, and the analgesic effect in the treatment group lasts a longer time, running a lower risk of losing effectiveness compared to the control group (P<0.05). Conclusion Real-time acupuncture treatment after the operation is significantly effective in relieving pain after operation for hemorrhoids.