OBJECTIVE： To establish method for simultaneous determination of 7 related substances in solifenacin succinate raw material. METHODS： HPLC method was adopted. The determination was performed on Thermo Hypersil ODS C18 column with mobile phase consisted of 0.02 mol/L KH2PO4 （0.02％ triethylamine， pH＝3.0）-acetonitrile （gradient elution） at the flow rate of 1.2 mL/min. The detection wavelength was set at 210 nm， and column temperature was 40 ℃. The sample size was 20 μL. The regression equation of solifenacin succinate and impurity A， C， D， I， J， K， L were drawn. Correction factors of impurities to solifenacin succinate were calculated with slope. The contents of impurities A， C， D， I， J， K and L were determined in 3 batches of solifenacin succinate raw material. RESULTS： The linear ranges of impurity A， C， D， I， J， K and L were 0.148 1-0.740 3， 0.142 9-0.714 5， 0.141 1-0.705 6， 0.148 9-0.744 6， 0.152 0-0.759 9， 0.137 9-0.689 6， 0.020 0-0.100 0 μg/mL （r＝0.999 8， 0.999 9 or 1.000 0）， respectively. The relative correction factors were 0.51， 0.40， 0.41， 0.91， 0.47， 0.85， 1.23. The limits of detection were 0.049 3， 0.047 6， 0.047 0， 0.048 1， 0.050 7， 0.046 0， 0.006 7   μg/mL. The quantification limits were 0.148 1， 0.142 9， 0.141 1， 0.148 9， 0.152 0， 0.137 9， 0.020 0 μg/mL， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 5.0％ （n＝6）. Average recoveries were 101.09％， 97.58％， 93.77％， 98.56％， 99.68％， 97.07％ and 93.54％； RSDs were 0.75％ ， 0.51％， 0.47％， 0.84％， 0.70％， 0.75％， 1.21％ （n＝9）. The contents of impurity I in 3 batches of solifenacin succinate raw material were 0.015％-0.018％， other impurities were not detected. CONCLUSIONS： The method is sensitive， accurate and reliable， which can be used to determine the related substances of solifenacin succinate raw material.

Objective To evaluate the interventional of bare-handed core muscle strength training and the sling exercise therapy (SET) on low back pain.Methods The soldiers of an Air Force Station who suffered from low back pain participated in the study.One hundred and forty-four patients were equally divided into three groups: the core muscle strength group (using core muscle strength training and the general physical therapy), the SET group (using SET and general physical therapy), and the control group (using the general physical therapy).All the groups were tested for 6 weeks.Questionnaires were distributed to the three groups before intervention, two weeks, four weeks and six weeks after intervention in order to find out about the degree of LBP, lumbar function and activity of these cases.Changes in the lumbar muscle strength were observed.Results The number of the patients who completed the test included 43 cases in the bare-handed core muscle strength group, 43 cases in the SET group and 44 cases in the control group, respectively, the VAS scores were 3.25, the Roland scores were 5.83,the FFD scores were 8.58 of the bare-handed core muscle strength group;the VAS scores were 3.30, the Roland scores were 5.93,the FFD scores were 8.10 of the SET group;the VAS scores were 2.41, the Roland scores were 3.03,the FFD scores were 7.40 of the control group.The LBP intervention efficiency of the three groups was 90.90%, 85.13% and 46.82%, respectively.There was no significant difference in indexes between the test groups(P>0.05).But compared with the control group,there was significant difference(P<0.05).Conclusion The bare-handed core muscle strength training and SET have obvious effects on LBP cases.According to their own infrastructure and facilities, military health care organizations in different environments can intervene in LBP cases with the bare-handed core muscle strength training or SET to alleviate the pain effectively.

Objective To compare the accuracy of the seasonal time series decomposition method and autoregressive integrated moving average (ARIMA) in the prediction of incidence of tuberculosis(TB) in order to facilitate early-warning.Methods The seasonal decomposition model and ARIMA model were constructed by SPSS20.0 software based on time series of monthly TB incidence between January 2005 and December 2014 in Urumqi,China.The obtained models were used to forecast the monthly incidence in 2015 and compared with the actual incidence respectively.Results Between 2005 and 2014,the incidence of TB was higher during March,April and May in Urumqi.A linear fitting model and a cubic curve fitting model were constructed by the time series seasonal decomposition method.The mean absolute percentage error (MAPE) of each predicted monthly incidence in 2015 was 18.75% and 92.25%,respectively.The predicted values of the linear model were lower than actual values and the predicted values of the cubic curve model were higher than actual values.An ARIMA (2,1,1) (1,1,0)12 fitting model was established by ARIMA method.The MAPE of each predicted monthly incidence in 2015 was 9.46% and there were no significant differences between the predicted and actual values.Conclusion The ARIMA method is better than the seasonal decomposition method for predicting the monthly incidence of TB in Urumqi.

Objective To analyze the epidemiologic characteristics and epidemic intensity of measles in China between 2005 and 2013 , and to provide evidence for reasonable allocation of health resources and measles elimination in China . Methods Descriptive epidemiological methods were applied to measles surveillance data and demographic information during this study.Results A total of 597 594 cases, including 366 deaths, were reported from all over China , among whom 355 409 were males (59.47%) and 242 185 were females (40.53%).The average annual incidence was 5.07/100 000 and the average annual mortality was 0.0031/100 000.The majority of cases were children under 10 years old, accouning for 64.64%.Conclusion The incidence of measles in China is decreasing .Measles mainly occurrs in spring and there is significant difference between age and gender groups , and obvious regional differences are also found .

Risk assessment is critical to responses to public health emergencies .Practices and researches concerning risk assessment of public health emergencies in China′s military are still in the early stage .This paper summarizes the rele-vant concepts , categorization , assessment methods , and practices .Based on management requirements of risk assessment , suggestions are raised about how to strengthen the risk assessment capability of Armed Forces by formulating management rules, perfecting assessment methods and developing assessment tools .

Combining theory and practice at home and abroad in recent years ,construction plan of the military infectious diseases syndromic surveillance system was discussed from aspects of data collection and transmission , data analysis and early warning , incident response based on the core business requirements of syndromic surveillance for infectious diseases . The study provides the demand traction for the system construction .

Objective To analyze and evaluate the effect of ship cabin color matching on the human physiological parameters.Methods Ten healthy male volunteers,with an age range of 19-31 and normal visual acuity and color vision,were all trainees recruited for the study.Heart rate,systolic pressure,diastolic pressure,contrast visual acuity and contrast visual sensitivity of the trainees were determined under 6 different cabin colors.Color codes were put in the order of 2#,6#,5#,4#,3# and 1# in accordance with the color warmth,with 2# being the cold color,6# the sub-cold color,5# the neutral color with cold deflection,4# the neutral color with warm deflection,2# the sub-warm color and 1 # the warm color.The test time cycle of each color scheme was 21 days.The effects of 6 cabin color schemes on the physiological parameters of the trainees were analyzed and evaluated by using TOPSIS (technique for order preference by similarity to an ideal solution) and cluster analysis.Results (1) Heart rate,systolic pressure and diastolic pressure of the trainees did not change with the changes in color scheme (P ＞ 0.05).In the same color scheme,heart rate,systolic pressure and diastolic pressure all fluctuated within normal ranges,without statistical significance (P ＞ 0.05).(2) In the 6 color schemes,average contrast visual acuity fluctuated within the ranges of 0.01-0.03.In the 3# color scheme,contrast visual acuity was the highest (4.98 ± 0.06),while contrast visual acuity in the 1 # color scheme was the lowest(4.95 ± 0.05).No statistical significance in contrast visual acuity could be seen in 6 color schemes(P ＞0.05).(3)In six different color environment schemes,there was statistical significance in contrast visual sensitivity,when the trainees were at 0.6 and 1.2 cycles per degree (P ＜0.05 and P ＜0.01).However,no statistical significance in contrast visual sensitivity could be noted at 2.4,4.8 and 9.6 cycles per degree(P ＞ 0.05).Conclusions Generally,heart rate,systolic pressure,diastolic pressure and contrast visual acuity of the trainees did not change with the changes in color schemes,while contrast visual sensitivity changed with the changes in color schemes of the cabin environment.The optimized sequence order of the 6 color schemes was 5#,6#,1#,3#,4# and 2#.The color scheme that had the least effect on the physiological parameters of the trainees was color scheme 5#,the neutral color with cold deflection.

Objective To analyze and evaluate the effect of ship cabin color matching on the human physiological parameters.Methods Ten healthy male volunteers,with an age range of 19-31 and normal visual acuity and color vision,were all trainees recruited for the study.Heart rate,systolic pressure,diastolic pressure,contrast visual acuity and contrast visual sensitivity of the trainees were determined under 6 different cabin colors.Color codes were put in the order of 2#,6#,5#,4#,3# and 1# in accordance with the color warmth,with 2# being the cold color,6# the sub-cold color,5# the neutral color with cold deflection,4# the neutral color with warm deflection,2# the sub-warm color and 1 # the warm color.The test time cycle of each color scheme was 21 days.The effects of 6 cabin color schemes on the physiological parameters of the trainees were analyzed and evaluated by using TOPSIS (technique for order preference by similarity to an ideal solution) and cluster analysis.Results (1) Heart rate,systolic pressure and diastolic pressure of the trainees did not change with the changes in color scheme (P ＞ 0.05).In the same color scheme,heart rate,systolic pressure and diastolic pressure all fluctuated within normal ranges,without statistical significance (P ＞ 0.05).(2) In the 6 color schemes,average contrast visual acuity fluctuated within the ranges of 0.01-0.03.In the 3# color scheme,contrast visual acuity was the highest (4.98 ± 0.06),while contrast visual acuity in the 1 # color scheme was the lowest(4.95 ± 0.05).No statistical significance in contrast visual acuity could be seen in 6 color schemes(P ＞0.05).(3)In six different color environment schemes,there was statistical significance in contrast visual sensitivity,when the trainees were at 0.6 and 1.2 cycles per degree (P ＜0.05 and P ＜0.01).However,no statistical significance in contrast visual sensitivity could be noted at 2.4,4.8 and 9.6 cycles per degree(P ＞ 0.05).Conclusions Generally,heart rate,systolic pressure,diastolic pressure and contrast visual acuity of the trainees did not change with the changes in color schemes,while contrast visual sensitivity changed with the changes in color schemes of the cabin environment.The optimized sequence order of the 6 color schemes was 5#,6#,1#,3#,4# and 2#.The color scheme that had the least effect on the physiological parameters of the trainees was color scheme 5#,the neutral color with cold deflection.

OBJECTIVETo review a group of clinical data of pedicle screw implantation in the treatment of middle-upper thoracic fracture and dislocation and explore these clinical effect.

METHODSThrough the retrospective analysis of 23 patients (31 vertebraes) of middle-upper thoracic fracture and dislocation included 21 male and 2 female,aged from 20 to 47 years in average of 33.5 years. The location and type of fractures were as follows: 2 in T2, 2 in T3, 5 in T4, 6 in T5, 10 in T6, 4 in T7, 2 in T8. Three cases were compression fractures, 7 burst fractures, 9 fracture-dislocations, 4 burst-dislocations.

RESULTSAfter a followed-up from 3 to 48 months, average 25.5 months, all cases had no serious complications. Anterior height of affected vertebral bodies had been restored from 40.4% to 90.3% after operation. Patients with incomplete spinal cord injuries improved one to two grades according to ASIA grade of spinal cord injury. Patients with complete spinal cord injuries improved 21.7 scores on average according to Sensory and Motion Scores.

CONCLUSIONMulti-segmental pedicle screw implantation for the injuries of vertebral body may get satisfactory reduction and fixation effects, and prevent the long-term loss of the vertebral body height and implanting failure.

Adult ; Bone Screws ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Joint Dislocations ; surgery ; Male ; Middle Aged ; Retrospective Studies ; Spinal Fractures ; surgery ; Thoracic Vertebrae ; injuries ; surgery

OBJECTIVE: To investigate the expressions and clinical significance of Hypoxia-Inducible Factor-1alpha (HIF-1alpha) and angiopoietin-2 (Ang-2) proteins in laryngeal squamous cell carcinoma. METHOD: Immunohistochemical technique was used to detect the expressions of HIF-1alpha and Ang-2 protein in 52 laryngeal squamous cell carcinoma and 10 normal tissues. RESULT: The positive rates of HIF-1alpha and Ang-2 proteins were significantly higher in laryngeal squamous cell carcinoma than those in normal tissues (P < 0.05). The expressions of HIF-1alpha protein was related with TNM stage and lymph node metastasis (P < 0.05), but had no marked correlations with the sex, age and histological differentiation (P > 0.05). The level of Ang-2 expression was also correlated with TNM stage, lymph node metastasis and histological differentiation of laryngeal squamous cell carcinoma. But not related to the age and sex. There was positive correlations between both expressions of HIF-1alpha and Ang-2 proteins (r = 0. 333, P < 0.01). CONCLUSION High expressions of HIF-1alpha and Ang-2 proteins in laryngeal squamous cell carcinoma may play an important role in tumor growth, invasion and metastasis.
Aged ; Angiopoietin-2 ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Case-Control Studies ; Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; metabolism ; Laryngeal Neoplasms ; metabolism ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged