Objective To explore the main points and preliminary efficacy of unilateral bi-portal spinal endoscopy(UBE)in the treatment of lumbar disc herniation(LDH).Methods From March 2022 to June 2023,patients admitted to the spinal surgery department for LDH who underwent UBE surgery were divided into traditional UBE technique group and UBE targeting technique group according to different surgical methods.The traditional UBE technology group revealed the entire interlaminar space,while the targeting technique group revealed part of the interlaminar space.ODI disability index,VAS score of lower extremity and low back,and intraoperative and postoperative complications of the two groups were recorded before operation and at 1day,1 month,3months and 6months post-operative-ly.Results A total of 78 patients were included,including 34 patients in the UBE traditional technique group,aged 40.1±12.2 years,16males and 18 females;44 patients in the UBE targeting technique group,aged 39.7±11.3 years,28males and 16 females.There was no significant difference in the basic data between the two groups.The operations were completed as planned.The operation time was 72.03±18.39min vs 46.2±11.85min,and the blood loss was 36.3±10.5ml vs 19.5±5.7ml,respectively.There were no significant differences in ODI disability index,VAS scores of back and lower extremities at 1day before surgery between the two groups.The dosage of postoperative painkillers in the traditional technique group was higher than that in the target technique group,and the VAS score and ODI index of low back pain on the first day after surgery were significantly higher than those in the target group(P＜0.05),but there was no significant difference between the two groups of patients with lower extremity pain,both of which were significantly relieved.There was no significant difference in lumbar and lower extremity pain VAS between the two groups at 1 month,3months and 6months after surgery,but ODI in the traditional technique group was significantly higher than that in the targeting technique group at 1 month and 3 months after surgery.Six months after surgery,the remission rate of modified Macnab criteria in the targeting technique group was 91.5％,and that of the traditional technique group was 89.5％without significant difference(P＞0.05).Cerebrospinal fluid leakage occurred in 1 case in the traditional technique group,and hematoma compression of nerves occurred in 2 cases in the targeting technique group after extubation,which were relieved after conservative treatments.Conclusion UBE targeting technique is more minimally invasive than traditional UBE technique in the treatment of lumbar disc herniation,which is helpful for rapid recovery after surgery.

Objective To explore the main points and preliminary efficacy of unilateral bi-portal spinal endoscopy(UBE)in the treatment of lumbar disc herniation(LDH).Methods From March 2022 to June 2023,patients admitted to the spinal surgery department for LDH who underwent UBE surgery were divided into traditional UBE technique group and UBE targeting technique group according to different surgical methods.The traditional UBE technology group revealed the entire interlaminar space,while the targeting technique group revealed part of the interlaminar space.ODI disability index,VAS score of lower extremity and low back,and intraoperative and postoperative complications of the two groups were recorded before operation and at 1day,1 month,3months and 6months post-operative-ly.Results A total of 78 patients were included,including 34 patients in the UBE traditional technique group,aged 40.1±12.2 years,16males and 18 females;44 patients in the UBE targeting technique group,aged 39.7±11.3 years,28males and 16 females.There was no significant difference in the basic data between the two groups.The operations were completed as planned.The operation time was 72.03±18.39min vs 46.2±11.85min,and the blood loss was 36.3±10.5ml vs 19.5±5.7ml,respectively.There were no significant differences in ODI disability index,VAS scores of back and lower extremities at 1day before surgery between the two groups.The dosage of postoperative painkillers in the traditional technique group was higher than that in the target technique group,and the VAS score and ODI index of low back pain on the first day after surgery were significantly higher than those in the target group(P＜0.05),but there was no significant difference between the two groups of patients with lower extremity pain,both of which were significantly relieved.There was no significant difference in lumbar and lower extremity pain VAS between the two groups at 1 month,3months and 6months after surgery,but ODI in the traditional technique group was significantly higher than that in the targeting technique group at 1 month and 3 months after surgery.Six months after surgery,the remission rate of modified Macnab criteria in the targeting technique group was 91.5％,and that of the traditional technique group was 89.5％without significant difference(P＞0.05).Cerebrospinal fluid leakage occurred in 1 case in the traditional technique group,and hematoma compression of nerves occurred in 2 cases in the targeting technique group after extubation,which were relieved after conservative treatments.Conclusion UBE targeting technique is more minimally invasive than traditional UBE technique in the treatment of lumbar disc herniation,which is helpful for rapid recovery after surgery.

Objective To investigate the popularization of cardiopulmonary resuscitation(CPR)knowledge and science popularization needs among urban and rural residents in Tonghai County,Yuxi City,Yunnan Province,so as to explore the establishment of an efficient and appropriate science popularization model.Methods A total of 300 residents aged 15-60 years old were selected from Tonghai County,Yuxi City,Yunnan Province using stratified and simple random sampling methods.A self-designed questionnaire was used to conduct an anonymous questionnaire survey.Results Only 20.3%of Tonghai County residents master CPR skills,and 26.2%of Tonghai County residents have never heard of CPR.There is a statistically significant difference in the awareness rate of CPR between rural residents and non-rural residents(P<0.01).There are differences in residents'age and CPR awareness(P<0.01),the age and CPR are inversely proportional.The residents have a higher willingness to perform chest compressions and mouth-to-mouth resuscitation on strangers,66.2%and 68.6%respectively.63.79%of residents have never attended relevant training.But 92.76%of the people said they were willing to participate in the relevant training when they learned the training news.Conclusion Residents in Tonghai County generally lack knowledge of CPR first aid,but the demand for first aid knowledge of residential CPR is high and the attitude towards rescue is positive.It is recommended that relevant departments increase CPR science popularization and training efforts,and popularize CPR into villages.

Objective:To study the clinical effects of modified surgical method with H-uvulopalatopharyngoplasty(H-UPPP)and traditional surgical method in the treatment of obstructive sleep apnea hypopnea syndrome(OSAHS)in children.Methods:The clini-cal data of 364 pediatric patients with OSAHS were retrospectively analyzed.The patients were divided into 2 groups and treated by low-temperature plasma knife radio frequency ablation for bilateral tonsil removal combined with H-UPPP(group A,n=204)and low-temperature plasma knife radio frequency ablation only(group B,n=160)respectively.The operative time,intraoperative bleed-ing,postoperative secondary bleeding,VAS of pain following operation,postoperative traumatic white film shedding time,OAH1,LSaO2,ESS scores and complications of the 2 groups were compared.Results:In group A the operative time,intraoperative bleed-ing,VAS,OAHI and ESS scores were lower than those in group B(P＜0.05),wile postoperative traumatic white film detachment time and LSaO2 were higher(P＜0.05).No statistically significant difference in terms of the number of cases of postoperative seconda-ry bleeding and complications was observed between the 2 groups(P＞0.05).Conclusion:The combination of low-temperature plas-ma radio frequency ablation and H-UPPP for the treatment of OSAHS may provide clear intraoperative view,and may improve the treatment effects.

Laboratory animals provide an important experimental resource for life science research,and their uniformity plays a key role in the accuracy and reliability of the experimental result.Genetic quality testing of laboratory animals is thus essential to evaluate the genetic quality of laboratory animals.Progress in modern biotechnology has led to improvements in the method of genetic testing of experimental animals.Single nucleotide polymorphisms,as molecular markers,have been widely used in genetic testing of laboratory animals,and method for their detection in laboratory animals have been updated,in line with biotechnology advances.In this paper,we review current research progress and the application of SNP detection method for genetic quality testing of laboratory animals,and discuss the advantages and disadvantages of various detection method,with a view to providing a reference basis for genetic quality testing of laboratory animals.

Laboratory animals provide an important experimental resource for life science research,and their uniformity plays a key role in the accuracy and reliability of the experimental result.Genetic quality testing of laboratory animals is thus essential to evaluate the genetic quality of laboratory animals.Progress in modern biotechnology has led to improvements in the method of genetic testing of experimental animals.Single nucleotide polymorphisms,as molecular markers,have been widely used in genetic testing of laboratory animals,and method for their detection in laboratory animals have been updated,in line with biotechnology advances.In this paper,we review current research progress and the application of SNP detection method for genetic quality testing of laboratory animals,and discuss the advantages and disadvantages of various detection method,with a view to providing a reference basis for genetic quality testing of laboratory animals.

Common iliac artery ureteral fistula is a rare but potentially life-threatening disease which is difficult to diagnose clinically. This paper reports a case of common iliac artery ureteral fistula. The patient was admitted to our hospital due to ureterostomy bleeding for one day. He underwent radical cystectomy and bilateral ureterostomy for bladder cancer 4 years ago, and also underwent radiotherapy and bilateral ureteral stents indwelling after the operation. Angiography revealed a left common iliac artery pseudoaneurysm, and a left common iliac artery ureteral fistula was considered. The left common iliac artery stent-graft was implanted. The patient recovered well after the operation, and there was no obvious hematuria during follow-up period of 6 months.

Objective:To establish a noninvasive prediction model for liver fibrosis in chronic hepatitis B (CHB) virus infection patients with alanine aminotransferase (ALT) less than upper limit of normal level.Methods:A total of 183 CHB patients with ALT <40 U/L admitted in the First Affiliated of Wenzhou Medical University from January 2014 to September 2017 were enrolled. There were 149 cases of non-marked liver fibrosis (F0-F2) and 34 cases of marked liver fibrosis (F3-F6) according to the Ishak scoring system. The clinical data, liver stiffness measurement (LSM), liver ultrasound imaging, serum biochemical features and hepatitis B virus related indexes of patients were retrospectively analyzed. The independent predictors of liver fibrosis were screened and a prediction model was constructed based on the results of multivariate logistic regression analysis. The receiver operating characteristic (ROC) curve was applied to evaluate the established model and other indicators in predicting liver fibrosis.Results:Multivariate logistic regression analysis showed that LSM ( OR=1.327, 95% CI 1.149-1.531), liver ultrasound scores ( OR=6.610, 95% CI 2.704-16.156) were independent predictors of liver fibrosis. The area under ROC curve(AUC)of the established model (LU) in predicting liver fibrosis was 0.873(95% CI 0.799-0.947), which was significantly greater than that of the ultrasound score, LSM, FIB-4, APRI and GPR (AUC=0.790, 0.804, 0.654, 0.673 and 0.770; Z=3.394, 1.982, 2.077, 3.168 and 2.165, all P<0.05 or <0.01). With the cut-off value of -1.787, the sensitivity and specificity of LU model were 85.3% and 77.2%, respectively. Conclusion:The established model (LU) can effectively predict the liver fibrosis in CHB patients with ALT less than upper limit of normal.

Objective To investigate the therapeutic effects and mechanisms of interleukin-10 (IL-10) gene-modified dendritic cells (DC-IL-10) in mice with liver fibrosis. Methods DC-IL-10 was constructed in vitro, the phenotype and function of which were evaluated by flow cytometry. BALB/c mice were treated with intraperitoneal injection of carbon tetrachloride(CCl4)to establish liver fibrotic model. DC-IL-10 was administrated via tail vein. Animals were divided into 4 groups including normal dendritic cell (DC) control , liver fibrosis only, negative lentiviral transfection DC (DC-mock) and DC-IL-10. Liver function, cytokine secretion, T lymphocyte differentiation and liver histomorphology were tested. Real-time PCR and western blot were used to analyze the effect of DC-IL-10 on Wnt/β-catenin signaling pathway and its role in liver fibrosis. Results When compared with DC control and DC-mock, the expression of DC-IL-10 surface stimulating molecules (major histocompatibity complex-Ⅱ, CD80, CD86) were significantly decreased (F=14.708, 22.503, 12.595, respectively, all P<0.05), and DC-IL-10 significantly inhibited T lymphocyte proliferation (F=50.295, P<0.05). When compared with liver fibrosis group, serum alanine aminotransferase and aspartate transaminase were decreased in DC-IL-10 treated group (all P<0.05), other parameters including inflammatory factors (tumor necrosis factor α, IL-6, IL-1β) reduced (all P<0.05), the proportion of regulatory T cells (Treg) increased (F=6.742, P<0.05), pathological damage improved, the expression of Wnt3a, α-SMA and β-catenin mRNA and protein significantly reduced in DC-IL-10 treatment group(all P<0.001). Conclusions DC-IL-10 induces elevation of Treg for immune tolerance, as well as inhibition of inflammatory response, block of Wnt/β-catenin signaling pathway, which translates into improvement of liver fibrosis.

Objective To investigate the impact of suppressor of cytokine signaling 1 (SOCS1) overexpression on dendritic cells (DC) functions and its therapeutic effect on acute liver failure (ALF) in mice.Methods Bone marrow derived dendritic cells (BMDC) from C57BL/6 mice were transfected with lentivirus encoding SOCS1 and negative control lentivirus at a MOI=50, and labeled as DC-SOCS1and DC-VNG, respectively after 96 hours of successful transduction.Then DCs were stimulated with lipopolysaccharides(LPS)1 mg/L and collected for flow cytometry analysis of surface costimulatory molecules, allogeneic mixed lymphocyte reaction (MLR) and western blot test of Janus kinase (JAK)/signaling transducers and activators of transcription (STAT) pathway.Afterwards, 90 mice were randomly assigned into 4 groups including 12 in normal control group, 26 in ALF group, 26 in treatment groups with DC-SOCS1 and 26 with the treatment of DC-VNG.All were received tail vein injection with normal saline, modified DC-VNG and DC-SOCS1 suspended in normal saline, respectively.Twelve hours after injection, LPS (10 μg/kg)/D-GaIN (600 mg/kg) were injected intraperitoneally to induce ALF model.The mortality, serum levels of alanine aminotransferase (ALT) and aspartate transaminase (AST), liver pathology and proportion of splenic regulatory T cells of each group were observed.Means in different groups were compared with one-way ANOVA analysis.Categorical variables were analyzed with x2 test.Variables were examined with normality test and homogeneity of variance with LSD test.Results The results of mixed lymphocyte reaction (MLR) revealed that T cell proliferation ratio in DC-SOCS1 group with mixture ratio of 100∶1 were (25.87±0.38)%, which was lower than that of mixture ratio of 10∶1 in the mDC group ([84.29±3.25]%) with statistical significance (x2=49.821, P<0.01);interleukin (IL)-10 concentration was higher than that in mDC group with mixture ratio of 10∶1 with statistical significance (F=20.112, P<0.05);IL-6 concentration was also lower with statistical significance (F=47.718, P<0.05).Compared to imDC, expression of JAK2 (t=0.525,0.523 and 0.489, respectively, all P<0.01), signal transduction factors and activation of transcription factors-1 (STAT1) (t=0.442,0.400 and 0.402, respectively, all P<0.01) and SOCS1 (t=0.322,0.363 and 1.090, respectively, all P<0.01) of mDC, DC-VNG and DC-SOCS1 after LPS stimulation increased significantly.Furthermore, the expressions of phosphorylated STAT1 (p-STAT1) and phosphorylated JAK2 (p-JAK2) of DC-SOCS1 were much lower than those of the mDC, with statistically significant difference (t=-3.840 and 0.254, respectively, both P<0.01).Pathological analysis revealed that there existed moderate hepatic cells necrosis and less immune cell infiltration in DC-SOCS1 group accompanied with higher regulatory T lymphocytes proportion than those in ALF group and DC-VNG group.Survival rate of ALF with DC-SOCS1 treatment group was significantly higher than that of ALF group with statistical difference (x2=12.87, P<0.05).Conclusions DC-SOCS1could sustain an immature state and exhibit as regulatory DC through negative regulation of JAK2/STAT1 pathway with overexpression of SOCS1.Infusion of DC-SOCS1 could ameliorate ALF by inhibiting aggressive inflammation response with increased proportion of regulatory T cells in mice, which shows good therapeutic effect for ALF mice.