OBJECTIVE To establish a quantitative analysis model for the contents of tussilagone， moisture， ethanol-soluble extract and total ash in Farfarae Flos based on near-infrared spectroscopy （NIRS）， providing a new idea for the rapid quality evaluation of Farfarae Flos and its preparations. METHODS Referring to the 2020 edition of the Chinese Pharmacopoeia， the contents of the main quality control indexes tussilagone， moisture， ethanol-soluble extract and total ash in 130 batches of Farfarae Flos from 19 producing areas were determined by HPLC， drying method， hot dip method and ash assay， respectively. The NIRS data information of the medicinal herbs of Farfarae Flos was collected， and then NIRS combined with the partial least squares method was used to establish the individual quantitative analysis models of the above quality control indexes in the samples， and the predictive model of the NIRS content was obtained after sample validation with validation set. RESULTS The range for the contents of tussilagone， moisture， ethanol-soluble extract and total ash in 130 batches of Farfarae Flos were 0.051 4%-0.103 5%， 7.75%-10.93%， 20.17%-31.12%， and 7.68%-12.10%， respectively. The internal cross-validation coefficients of determination （R2） of the established models for the quantitative analysis of tussilagone， moisture， ethanol-soluble extract and total ash in Farfarae Flos were 0.985 8， 0.968 4， 0.973 4， 0.988 0， respectively； the root mean square errors of calibration （RMSEC） were 0.001 54， 0.187， 0.478， 0.127， respectively； the root mean square errors of prediction （RMSEP） were 0.001 81， 0.212， 0.543， 0.149， respectively； RMSEP/RMSEC were 1.175 3， 1.133 7， 1.136 0 and 1.173 2， respectively， which were all within a reasonable range （1＜RMSEP/RMSEC≤1.2）. The mean absolute errors between the true and model-predicted values of the above four quality control indexes in the validation set of samples were －0.000 36， 0.061 43， 0.144 00， and 0.010 43， respectively，and the mean predicted recoveries were 99.65%， 100.72%，100.66%， and 100.15%， respectively. CONCLUSIONS The established NIRS quantitative analysis model has high stability and reliable results， which can be used for the rapid batch prediction of the content of relevant quality control indexes in Farfarae Flos.

Objective:To study the clinical effects of modified surgical method with H-uvulopalatopharyngoplasty(H-UPPP)and traditional surgical method in the treatment of obstructive sleep apnea hypopnea syndrome(OSAHS)in children.Methods:The clini-cal data of 364 pediatric patients with OSAHS were retrospectively analyzed.The patients were divided into 2 groups and treated by low-temperature plasma knife radio frequency ablation for bilateral tonsil removal combined with H-UPPP(group A,n=204)and low-temperature plasma knife radio frequency ablation only(group B,n=160)respectively.The operative time,intraoperative bleed-ing,postoperative secondary bleeding,VAS of pain following operation,postoperative traumatic white film shedding time,OAH1,LSaO2,ESS scores and complications of the 2 groups were compared.Results:In group A the operative time,intraoperative bleed-ing,VAS,OAHI and ESS scores were lower than those in group B(P＜0.05),wile postoperative traumatic white film detachment time and LSaO2 were higher(P＜0.05).No statistically significant difference in terms of the number of cases of postoperative seconda-ry bleeding and complications was observed between the 2 groups(P＞0.05).Conclusion:The combination of low-temperature plas-ma radio frequency ablation and H-UPPP for the treatment of OSAHS may provide clear intraoperative view,and may improve the treatment effects.

Common iliac artery ureteral fistula is a rare but potentially life-threatening disease which is difficult to diagnose clinically. This paper reports a case of common iliac artery ureteral fistula. The patient was admitted to our hospital due to ureterostomy bleeding for one day. He underwent radical cystectomy and bilateral ureterostomy for bladder cancer 4 years ago, and also underwent radiotherapy and bilateral ureteral stents indwelling after the operation. Angiography revealed a left common iliac artery pseudoaneurysm, and a left common iliac artery ureteral fistula was considered. The left common iliac artery stent-graft was implanted. The patient recovered well after the operation, and there was no obvious hematuria during follow-up period of 6 months.

Urease decomposes urea to ammonia, and has application potential in agriculture and medical treatment. Urease proteins include structural proteins (UreA, UreB and UreC) and accessory proteins (UreD/UreH, UreE, UreF and UreG), each of them has its own unique role in urease maturation. The structural proteins form the active center of urease, and the accessory proteins are responsible for the delivery of nickel. We review here the structure and function of bacterial urease complexes, and how each protein interacts to complete the activation process. We hope to provide theoretical basis for the regulation of urease activity and the development of urease inhibitors.
Bacterial Proteins ; Nickel ; Urease ; metabolism

No abstract available.
Leprosy*

Objective To investigate the clinical efficacy of comprehensive hyperbaric oxygen (HBO) in the treatment of the patients with sudden hearing loss .Methods Totally, 80 patients with sudden hearing loss who were admitted into our hospital for treatment from June 2015 to March 2017 were selected as research subjects, and were divided into the observation group (n =40) and the control group (n =40), in accordance with the sequence of admission.The control group was given volume expansion to improve hemorheology , vasodilator to improve microcirculation, glucocorticoids, neurotrophic drugs and ginkgo biloba extract dripping pills, while the observation group received HBO in addition to the treatment received by the control group .One course of treatment for all patients in the 2 groups was 10 days, and following 3 courses of treatment, changes in hearing and clinical efficacy before and after treatment for the patients of the 2 groups were compared between the 2 groups.Results Following treatment for 3 courses for the observation group , 4 cases were cured, 21 cases were markedly effective , 10 cases were effective and 5 cases were ineffective, with a total effective rate of 87.5％, while for the control group, no cases were cured, 5 cases were markedly effective, 22 cases were effective and 13 cases were ineffective, with a total effective rate of 67.5％.Statistical significance could be seen, when comparisons were made between the observation group and the control group (P <0.05).Before treatment, there was no significant differences in full frequency hearing loss for the patients of 2 groups (P >0.05).After 2 courses of treatment, the value of low frequency hearing for the observation group increased more significantly, as compared with that of the control group [(37.39 ±5.77) vs (23.91 ±4.08) dB,P <0.05], however, there were no statistical differences in the increased values of high frequency hearing , when comparisons were made between the 2 groups (P >0.05).After 3 courses of treatment, the increased values of both low frequency and high frequency hearing for the patients of the observation group were all significantly better than those of the control group [ low frequency: (45.99 ±5.98) vs (36.79 ±5.72) dB; high frequency:(19.63 ±3.94) vs (9.70 ±2.17)dB, P <0.05].Conclusions Comprehensive HBO treatment could effectively improve the hearing level of the patients with full frequency sudden hearing loss , which was worth further clinical popularization .

Objective To investigate the clinical efficacy of comprehensive hyperbaric oxygen (HBO) in the treatment of the patients with sudden hearing loss .Methods Totally, 80 patients with sudden hearing loss who were admitted into our hospital for treatment from June 2015 to March 2017 were selected as research subjects, and were divided into the observation group (n =40) and the control group (n =40), in accordance with the sequence of admission.The control group was given volume expansion to improve hemorheology , vasodilator to improve microcirculation, glucocorticoids, neurotrophic drugs and ginkgo biloba extract dripping pills, while the observation group received HBO in addition to the treatment received by the control group .One course of treatment for all patients in the 2 groups was 10 days, and following 3 courses of treatment, changes in hearing and clinical efficacy before and after treatment for the patients of the 2 groups were compared between the 2 groups.Results Following treatment for 3 courses for the observation group , 4 cases were cured, 21 cases were markedly effective , 10 cases were effective and 5 cases were ineffective, with a total effective rate of 87.5％, while for the control group, no cases were cured, 5 cases were markedly effective, 22 cases were effective and 13 cases were ineffective, with a total effective rate of 67.5％.Statistical significance could be seen, when comparisons were made between the observation group and the control group (P <0.05).Before treatment, there was no significant differences in full frequency hearing loss for the patients of 2 groups (P >0.05).After 2 courses of treatment, the value of low frequency hearing for the observation group increased more significantly, as compared with that of the control group [(37.39 ±5.77) vs (23.91 ±4.08) dB,P <0.05], however, there were no statistical differences in the increased values of high frequency hearing , when comparisons were made between the 2 groups (P >0.05).After 3 courses of treatment, the increased values of both low frequency and high frequency hearing for the patients of the observation group were all significantly better than those of the control group [ low frequency: (45.99 ±5.98) vs (36.79 ±5.72) dB; high frequency:(19.63 ±3.94) vs (9.70 ±2.17)dB, P <0.05].Conclusions Comprehensive HBO treatment could effectively improve the hearing level of the patients with full frequency sudden hearing loss , which was worth further clinical popularization .

Objective To analyze the malaria epidemiological characteristics in Tengchong City from 2010 to 2015,so as to provide the evidence for adjusting and formulating measures in the elimination stage. Methods The malaria data were collected and analyzed by using Microsoft Excel 2010 in Tengchong City from 2010 to 2015. Results There were 1 408 malaria cases re?ported in Tengchong City from 2010 to 2015,including 1 091 cases of Plasmodium vivax infection,256 cases of P. falciparum infection,5 cases of P. malariae infection,1 case of P. ovale infection,1 case of mixed infections,and 54 unclassified cases. Totally 1 390 imported cases were recorded and 98.06%of them(1 363/1 390)were imported from Myanmar. Most of the pa?tients(n=908)were aged from 21 to 40 years,and the male to female ratio was 11.03∶1. The highest?risk populations were farmers and migrant workers. The most cases were observed in April and June,and at that time,most of the floating workers re?turned. Conclusion Imported malaria is severe in Tengchong City,and there is a great challenge to malaria elimination.

Objective To study the prevalence of tick bites and relative factors of outpatients with fever from health care fa?cilities in Tengchong County. Methods From July to August,2014,the outpatients with fever in five health care facilities namely Tengchong Center for Disease Control and Prevention,Hehua Health Center,Jietou Health Center,Qushi Health Cen?ter and Xinhua Health Center were investigated by questionnaires. The factors related to tick bites were explored with the univari?ate analysis and multiple regression models. Results Totally 884 effect questionnaires were acquired. Among the 884 partici?pants,85(9.6%)had experienced tick bites. The frequency of tick bites was associated with locations,gender,age group,own?ership of cattle,working at foreign country,firewood cutting and lumbering in the forest(all P<0.05). The multiple regression revealed that the male and people raising cattle,working at foreign country,engaging in firewood cutting and lumbering in the forest had a more risk for tick bites. Conclusions Tick bites are common in the residents of Tengchong County. The risk of be?ing bitten varies in different populations. The local health departments should promote health education in the high?risk popula?tion to reduce the risk of infecting tick?borne diseases.

A brief introduction to the development milestones of postgraduate medical education in the world, highlighting the present details of such systems in the United States, United Kingdom, France, Germany and some Asian countries.It is held that common patterns can be identified despite the gaps found in such systems of these countries.In view of the training management system cornmonalities of thecountries,the authors proposed the development directions of residency training in China to enhance thehands-on capability and competence of clinical doctors in a standardized manner.