Objective:To develop national standard materials for Streptococcus pneumoniae serotypes 12F,14,17F and 22F polysaccharides.Methods:According to the method recommended by the 2020 Chinese Pharmacopoeia,the relevant limit quality control of four different serotypes of polysaccharide candidate standards was conducted.The uniformity of subpackage and stability of the candidate standard products under different conditions were ana-lyzed.Furthermore,three laboratories were organized for collaborative calibration.Results:The results showed that the quality of serotypes 12F,14,17F and 22F polysaccharides candidate standards met the requirements of the quality standard of polysaccharide in the 2020 edition of the Chinese Pharmacopoeia.The RSD between polysaccha-ride content of different candidate standard samples were less than 5.0％,indicating that the uniformity of the poly-saccharide candidate standards was better.The results from stability analysis confirmed that the polysaccharide con-centration of serotypes 12F,14,17F and 22F polysaccharide candidate standard did not change significantly after being placed at 2-8 ℃,25 and 37℃for different times and after repeated freezing and thawing three times.Compared with the test results of each candidate standard product stored at-70 ℃ for a long time,the RSD were all within 5％.The collaborative results confirmed that the RSD of the polysaccharide content in different laborato-ries were less than 12％,of which assigned the concentration of four different serotype polysaccharide candidate standards.Conclusion:Serotypes 12F,14,17F and 22F polysaccharide standards have been developed,of which can be used for quality control of pneumococcal polysaccharide vaccines in the future.

Objective:To develop national standard materials for Streptococcus pneumoniae serotypes 12F,14,17F and 22F polysaccharides.Methods:According to the method recommended by the 2020 Chinese Pharmacopoeia,the relevant limit quality control of four different serotypes of polysaccharide candidate standards was conducted.The uniformity of subpackage and stability of the candidate standard products under different conditions were ana-lyzed.Furthermore,three laboratories were organized for collaborative calibration.Results:The results showed that the quality of serotypes 12F,14,17F and 22F polysaccharides candidate standards met the requirements of the quality standard of polysaccharide in the 2020 edition of the Chinese Pharmacopoeia.The RSD between polysaccha-ride content of different candidate standard samples were less than 5.0％,indicating that the uniformity of the poly-saccharide candidate standards was better.The results from stability analysis confirmed that the polysaccharide con-centration of serotypes 12F,14,17F and 22F polysaccharide candidate standard did not change significantly after being placed at 2-8 ℃,25 and 37℃for different times and after repeated freezing and thawing three times.Compared with the test results of each candidate standard product stored at-70 ℃ for a long time,the RSD were all within 5％.The collaborative results confirmed that the RSD of the polysaccharide content in different laborato-ries were less than 12％,of which assigned the concentration of four different serotype polysaccharide candidate standards.Conclusion:Serotypes 12F,14,17F and 22F polysaccharide standards have been developed,of which can be used for quality control of pneumococcal polysaccharide vaccines in the future.

Objective To investigate the effect of dexmedetomidine combined with ropivacaine on postop-erative sleep quality in patients undergoing upper abdominal surgery following Oblique Subcostal Transversus Abdominis Plane Block(OSTAPB).Methods A total of 140 patients who underwent gastric surgery at the Affiliated Hospital of Xuzhou Medical University between September 2024 and November 2024 were enrolled.According to the random number table method,they were randomly allocated into two groups:the simple subcostal transversus abdominis plane block group(Group A)and the dexmedetomidine combined with subcostal transversus abdominis plane block group(Group B),with 70 patients in each group.The study compared the Asymptomatic Sleep Distur-bance Scale(AIS)scores and Self-rating Anxiety Scale(SAS)scores on the night before surgery,AIS scores on the night after surgery and the following day,the amount of remifentanil used during surgery,and the cumulative num-ber of patient-controlled analgesia(PCA)button presses within 3 days postoperatively.Additionally,the Numeric Rating Scale(NRS)pain scores at 2 h,6 h,12 h,24 h,and 48 h post-surgery,as well as the Quality of Recovery-15(QoR-15)scores on the night of surgery and the second day post-surgery,were evaluated.Results There were no significant differences in anxiety levels or subjective sleep quality scores between the two groups prior to the opera-tion(P>0.05).Additionally,there was no significant difference in the usage of remifentanil between Group A and Group B(P>0.05).The NRS scores for pain differed significantly between the two groups within the first 48 hours post-operation(P<0.05);however,no significant difference was observed in NRS scores between the two groups after 24 hours during rest.Furthermore,the number of patient-controlled analgesia(PCA)activations in Group B was significantly lower than that in Group A at 12 hours post-operation(P<0.05).Lastly,both the QoR-15 and sleep quality were significantly higher in Group B compared to Group A(P<0.05).Conclusion Dexmedetomidine combined with ropivacaine for subcostal transversus abdominis plane block not only provides superior postoperative analgesia compared to ropivacaine alone but also enhances patients' sleep quality and recovery quality on the night following surgery,thereby contributing to improved overall postoperative recovery.

Objective:To analyze effects of T11TS in the treatment of Cryptococcus neoformans fungal infection and the regula-tion of T-cell calcineurin(CaN)-activated T lymphoid nuclear factor(NFAT)pathway.Methods:SD rats infected with Cryptococcus neoformans were treated with immunoenhancer T11TS.Flow cytometry,Western blot and nuclear translocation were used to detect the changes of T cell function and related signal pathway.Results:The number of Cryptococcus neoformans in lung and spleen of CT3 group was significantly less than Cryptococcus neoformans infection group,CT1 group and CT2 group(P<0.05).The results of flow cy-tometry showed that the expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 in Cryptococcus neoformans infection group were significantly lower than control group(P<0.05).The expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 of CT1,CT2 and CT3 groups were significantly higher than Cryptococcus neoformans infection group(P<0.05).The results of Western blot were similar to those of flow cytometry.Conclusion:T11TS in the treatment of Cryptococcus neoformans infection can enhance the immunity of the body by up-regulating the expression of related signal factors in the CaN-NFAT pathway of T lymphocytes,increasing the expression of NFAT and IL-2,stimulating the activation and proliferation of T lymphocytes.

Objective:To analyze the correlation between serum uric acid-to-high-density lipoprotein cholesterol ratio (UHR) and risk of all-cause death or cardiovascular disease (CVD) death among urban and rural elderly in Beijing.Methods:Based on the Beijing Healthy Aging Cohort Study, 9 022 participants with complete baseline data were enrolled, and their survival and death outcomes were followed up. Multivariable Cox proportional hazard regression model were used to analyze the associations between the UHR level and the risks of all-cause mortality and CVD mortality.Results:As of March 31, 2021, the median follow-up time M( Q1, Q3) was 6.18 (5.36, 6.75) years. There were 1 166 all-deaths, with a death density of 19.26 per 1 000 person-years, and 562 CVD deaths, with a death density of 9.28 per 1 000 person-years. After adjusting sociodemographic characteristics and lifestyle factors, multivariable Cox proportional hazard regression model showed that the risk of all-cause mortality increased by 3% ( HR=1.03, 95% CI: 1.02-1.04) and the risk of CVD mortality increased by 4% ( HR=1.04, 95% CI: 1.02-1.06) for every 1% increase in UHR. Compared with the T1 group of UHR tertiles, the T3 group had a 42% increase in the risk of all-cause death ( HR=1.42, 95% CI: 1.22-1.66) and a 53% increase in the risk of CVD death ( HR=1.53, 95% CI: 1.21-1.94). Conclusions:The UHR level is significantly associated with the risks of all-cause mortality and CVD mortality among urban and rural older adults in Beijing. The UHR level may be one of the potential predictors of death risk in community-dwelling older adults.

Objective:To analyze effects of T11TS in the treatment of Cryptococcus neoformans fungal infection and the regula-tion of T-cell calcineurin(CaN)-activated T lymphoid nuclear factor(NFAT)pathway.Methods:SD rats infected with Cryptococcus neoformans were treated with immunoenhancer T11TS.Flow cytometry,Western blot and nuclear translocation were used to detect the changes of T cell function and related signal pathway.Results:The number of Cryptococcus neoformans in lung and spleen of CT3 group was significantly less than Cryptococcus neoformans infection group,CT1 group and CT2 group(P<0.05).The results of flow cy-tometry showed that the expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 in Cryptococcus neoformans infection group were significantly lower than control group(P<0.05).The expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 of CT1,CT2 and CT3 groups were significantly higher than Cryptococcus neoformans infection group(P<0.05).The results of Western blot were similar to those of flow cytometry.Conclusion:T11TS in the treatment of Cryptococcus neoformans infection can enhance the immunity of the body by up-regulating the expression of related signal factors in the CaN-NFAT pathway of T lymphocytes,increasing the expression of NFAT and IL-2,stimulating the activation and proliferation of T lymphocytes.

Objective: To explore intraoral scanning strategies for elastic impressions during the fabrication process of duplicate complete dentures and to investigate the accuracy of 3D scanning strategies on the surface of complete dentures. The goal is to utilize digital technology to improve the traditional fabrication methods of duplicate complete dentures. Methods: Eight sets of replicated denture model for edentulous patients were selected. Conventional complete dentures were created based on these models. The condition of the patient’s alveolar bone atrophy was simulated on these models, and elastic impressions were built using complete dentures as individual trays with polyether silicone rubber materials. TRIOS 3 intraoral scanners were used to scan the elastic impressions according to four scanning strategies (A: tissue surface - artificial teeth - polished surface of denture; B: artificial teeth - polished surface of denture - tissue surface; C: tissue surface - artificial teeth - polished surface of denture in powder spraying state; D: artificial teeth - polished surface - tissue surface in powder spraying state). The 3D data obtained by the desktop scanner were used as the reference. The maximum deviation, average deviation, and standard deviation of the 3D data models obtained by different scanning strategies were compared using the Geomagic Control X software. For the complete denture, the maximum deviation was 0.3 mm. The obtained results were analyzed by PASW Statistics 18 software. Results: The maximum deviation value of the maxillary scans in the 3D data compared with the desktop scanning data was (0.188 ± 0.109) mm, and that of the mandibular scans was (0.200 ± 0.099) mm. There was no statistically significant difference between them (t = 0.139, P = 0.624). However, the maximum deviation values of both the maxillary and mandibular scans were lower than the required maximum error (0.3 mm) for complete dentures in clinical practice, and the difference was statistically significant (P<0.001). The average deviations of the maxillary and mandibular models were (0.024 ± 0.212) mm and (0.014 ± 0.014) mm, respectively, and the differences were statistically significant (t = 4.228, P = 0.021). The standard deviations of the maxillary and mandibular models were (0.074 ± 0.032) mm and (0.074 ± 0.034) mm, respectively. There was no statistically significant difference between them (t = 0.813, P = 0.371). There were no statistically significant differences in the average deviations and standard deviations of each scanning strategy between the maxillary and mandibular impressions within and between groups. Comparing the deviation between the tissue surface and the polished surface of the 3D data of the upper and lower jaws on the oral scanner and the desktop scanner shows that the areas with larger deviations in the maxillary impressions were mainly concentrated in the maxillary tuberosity and palatal vault regions, and those in the mandibular impressions were mainly concentrated in the molar posterior pad region. Conclusion The digital impressions formed by intraoral scanning the maxillary and mandibular elastic impressions can meet the requirements for clinical fabrication of complete dentures. However, in clinical practice, special attention should be paid to checking and adjusting the fit of the maxillary tuberosity and palatal vault and the mandibular molar posterior pad areas of the complete dentures.

Objective To investigate the effect of dexmedetomidine combined with ropivacaine on postop-erative sleep quality in patients undergoing upper abdominal surgery following Oblique Subcostal Transversus Abdominis Plane Block(OSTAPB).Methods A total of 140 patients who underwent gastric surgery at the Affiliated Hospital of Xuzhou Medical University between September 2024 and November 2024 were enrolled.According to the random number table method,they were randomly allocated into two groups:the simple subcostal transversus abdominis plane block group(Group A)and the dexmedetomidine combined with subcostal transversus abdominis plane block group(Group B),with 70 patients in each group.The study compared the Asymptomatic Sleep Distur-bance Scale(AIS)scores and Self-rating Anxiety Scale(SAS)scores on the night before surgery,AIS scores on the night after surgery and the following day,the amount of remifentanil used during surgery,and the cumulative num-ber of patient-controlled analgesia(PCA)button presses within 3 days postoperatively.Additionally,the Numeric Rating Scale(NRS)pain scores at 2 h,6 h,12 h,24 h,and 48 h post-surgery,as well as the Quality of Recovery-15(QoR-15)scores on the night of surgery and the second day post-surgery,were evaluated.Results There were no significant differences in anxiety levels or subjective sleep quality scores between the two groups prior to the opera-tion(P>0.05).Additionally,there was no significant difference in the usage of remifentanil between Group A and Group B(P>0.05).The NRS scores for pain differed significantly between the two groups within the first 48 hours post-operation(P<0.05);however,no significant difference was observed in NRS scores between the two groups after 24 hours during rest.Furthermore,the number of patient-controlled analgesia(PCA)activations in Group B was significantly lower than that in Group A at 12 hours post-operation(P<0.05).Lastly,both the QoR-15 and sleep quality were significantly higher in Group B compared to Group A(P<0.05).Conclusion Dexmedetomidine combined with ropivacaine for subcostal transversus abdominis plane block not only provides superior postoperative analgesia compared to ropivacaine alone but also enhances patients' sleep quality and recovery quality on the night following surgery,thereby contributing to improved overall postoperative recovery.

ObjectiveTo investigate the mechanism by which Mingshi prescription regulates the retinal melanopsin-dopamine （Opn4-DA） axis in myopic mice to inhibit endoplasmic reticulum （ER） stress in the retina and sclera， thereby delaying axial elongation associated with myopia. MethodsSixty 4-week-old male SPF-grade C57BL/6J mice were randomly divided into a normal group， a form-deprived myopia group （FDM group）， an intrinsically photosensitive retinal ganglion cells ablation group （ipRGCs group）， a Mingshi Prescription group （MSF group， 5.2 g·kg^-1）， and an ipRGCs + MSF group （5.2 g·kg^-1）. Except for the normal group， all other groups underwent FDM modeling. Additionally， the ipRGCs and ipRGCs + MSF groups received retinal ipRGC ablation. Three weeks after modeling， the MSF and ipRGCs + MSF groups were administered Mingshi prescription via continuous gavage for six weeks. After refraction and axial length were measured in all mice， eyeballs were collected along with retinal and scleral tissues. Pathological and morphological changes in the retina， choroid， and sclera were observed using periodic acid-Schiff （PAS） staining. Western blot was employed to detect the relative protein expression levels of dopamine D1 receptor （DRD1）， C/EBP homologous protein （CHOP）， and glucose-regulated protein 78 （GRP78） in the retina， and CHOP and GRP78 in the sclera. Real-time PCR was used to detect the relative mRNA expression of Opn4， CHOP， and GRP78 in the retina， and CHOP and GRP78 in the sclera. Immunofluorescence staining （IF） was performed to detect the expression of Opn4 and DRD1 in retinal tissues. ResultsCompared with the normal group， the FDM group showed a significant myopic shift in refraction （P<0.05） and a significant increase in axial length （P<0.05）. The retinal layers were thinner， the number of ganglion cells was reduced， and collagen fibers in the sclera were loosely arranged with evident gaps. Opn4 and DRD1 protein and mRNA expression in the retina were significantly decreased （P<0.05）， while CHOP and GRP78 protein and mRNA expression in both retinal and scleral tissues were significantly increased （P<0.05）. Compared with the FDM group， the ipRGCs group exhibited further increases in myopic refraction and axial length （P<0.05）， more pronounced thinning and looseness in the retinal， choroidal， and scleral layers， lower expression of Opn4 and DRD1 protein and mRNA in the retina （P<0.05）， and higher expression of CHOP and GRP78 protein and mRNA in the retina and sclera （P<0.05）. Compared with the FDM group， the MSF group showed significantly reduced refractive error and axial length （P<0.05）， with improved cellular number， arrangement， and thickness in ocular tissues， increased Opn4 and DRD1 protein and mRNA expression in the retina （P<0.05）， and reduced CHOP and GRP78 protein and mRNA expression in both retina and sclera （P<0.05）. Similarly， the ipRGCs + MSF group showed significant improvements in terms of the above items compared with the ipRGCs group （P<0.05）. ConclusionMingshi Prescription delays myopic axial elongation and refractive progression by regulating the Opn4-DA axis in the retina of myopic mice， thereby inhibiting ER stress in the retina and sclera. This intervention promotes Qi and blood nourishment of the eyes， softens the fascia， and restores ocular rhythm.

Triple-negative breast cancer (TNBC) represents an aggressive breast cancer subtype with poor prognosis and limited targeted treatment options. This investigation examined the anti-cancer potential of Caerulomycin A (Cae A), a natural compound derived from marine actinomycetes, against TNBC. Cae A demonstrated selective inhibition of viability and proliferation in TNBC cell lines, including 4T1, MDA-MB-231, and MDA-MB-468, through apoptosis induction. Mechanistic analyses revealed that the compound induced sustained endoplasmic reticulum (ER) stress and subsequent upregulation of C/EBP homologous protein (CHOP) expression, resulting in mitochondrial damage-mediated apoptosis. Inhibition of ER stress or CHOP expression knockdown reversed mitochondrial damage and apoptosis, highlighting the essential role of ER stress and CHOP in Cae A's anti-tumor mechanism. Both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) decreased in TNBC cells following Cae A treatment, indicating reduced mitochondrial respiratory and glycolytic capacities. This diminished energy metabolism potentially triggers ER stress and subsequent apoptosis. Furthermore, Cae A exhibited significant anti-tumor effects in the 4T1 tumor model in vivo without apparent toxicity. The compound also effectively inhibited human TNBC organoid growth. These results indicate that Cae A may serve as a potential therapeutic agent for TNBC, with its efficacy likely mediated through the disruption of glucose metabolism and the induction of ER stress-associated apoptosis.
Humans ; Endoplasmic Reticulum Stress/drug effects* ; Triple Negative Breast Neoplasms/genetics* ; Apoptosis/drug effects* ; Cell Line, Tumor ; Female ; Animals ; Glucose/metabolism* ; Mice ; Cell Proliferation/drug effects* ; Transcription Factor CHOP/genetics* ; Antineoplastic Agents/pharmacology* ; Mitochondria/metabolism* ; Mice, Inbred BALB C