The caries-preventive effects of arginine have been attributed to its impact on biofilm composition and pH. Recent in vitro studies suggest that arginine also affects the production of biofilm matrix components that contribute to virulence, but this mechanism has not been investigated clinically. This randomized, placebo-controlled, triple-blind, split-mouth in situ trial assessed arginine's impact on the microbial composition, matrix architecture, and microscale pH of biofilms from caries-active patients (N = 10). We also examined whether individual differences in the pH response to arginine were related to biofilm composition and matrix structure. Biofilms were grown for four days on carriers attached to intraoral splints. Three times daily, the biofilms were treated extraorally with sucrose (5 min), followed by arginine or placebo (30 min), in a split-mouth design. After growth, the microscale biofilm pH response to sucrose was monitored by pH ratiometry. Microbial biofilm composition and carbohydrate matrix architecture were analyzed by 16S rRNA gene sequencing and fluorescence lectin-binding analysis, respectively. Arginine treatment significantly mitigated sucrose-induced pH drops, reduced total carbohydrate matrix production, and altered the spatial distribution of fucose- and galactose-containing carbohydrates. Both arginine- and placebo-treated biofilms were dominated by streptococci and Veillonella spp. Paired analyses showed a significant reduction in mitis/oralis group streptococci and a non-significant increase in several arginine metabolizers in arginine-treated biofilms. Individual pH responses were not significantly associated with the abundance of specific bacterial taxa or carbohydrate matrix components. In conclusion, arginine reduced the virulence of biofilms from caries-active patients through multiple mechanisms, including suppressing matrix carbohydrate production.
Biofilms/drug effects* ; Humans ; Arginine/pharmacology* ; Hydrogen-Ion Concentration ; Dental Caries/prevention & control* ; Male ; Female ; Adult ; Double-Blind Method ; Sucrose/pharmacology*

While driving, a driver is required to control the steering wheel to change direction. The driver’s muscles of the upper limbs and shoulders are involved in such a task. Therefore, an assessment of the driver’s physiology according to certain condition is necessary to improve driving comfort and safety. This study aims to investigate the driver’s Deltoid Anterior (DA) muscle activity while operating the steering wheel. Eleven test subjects were recruited for an experiment using a car simulator. They were required to remain in the car seat and perform the task of steering the wheel. Surface electromyography (SEMG) was used to record each subject’s muscle contraction while turning the steering wheel to the right and left by several degrees. According to the findings, 45 degrees turning recorded the highest Root mean Square (RMS) value for DA. In addition, DA muscle activation increased with more degrees turning.
Deltoid anterior ; steering wheel ; SEMG ; driver ; discomfort ; distant seated posture