Objective:To isolate and screen Lactobacillus crispatus from healthy female vagina and evaluate its probiotic characteristics in vitro in order to provide a new strain for further screening and study of microecological agents for effective treatment of female reproductive system diseases. Methods:The vaginal secretions of 10 healthy women were collected and screened in Department of Reproductive Medicine, the Second Affiliated Hospital of Naval Medical University from April 2023 to May 2023. Lactobacillus crispatus was identified by 16S rRNA sequence, and its growth characteristics, acid-producing ability, H 2O 2 producing ability and bacteriostatic ability were detected. Results:Totally 39 strains of Lactobacillus crispatus were isolated and identified from 10 healthy women. The logarithmic growth phase of Lactobacillus crispatus was 10-40 h, among which Lactobacillus crispatus 4-5 and Lactobacillus crispatus 6-7 grew faster. The pH of Lactobacillus crispatus decreased rapidly within 8-24 h, and the pH of 48 h was stable between 3.89 and 4.05. Among them, Lactobacillus crispatus 6-6, 6-7, 6-9, 6-11 had better acid production. All 10 strains of Lactobacillus crispatus could produce H 2O 2, and Lactobacillus crispatus 4-5, 4-11, 6-5 and 6-7 had better H 2O 2 production. Lactobacillus crispatus 6-5, 6-7 and 6-9 had better inhibitory effect on Candida albicans, in which the diameters of bacteriostatic zone were (20.90±0.31) mm, (20.61±0.70) mm and (21.73±0.37) mm, respectively. Lactobacillus crispatus 4-5 and 6-7 had better inhibitory effect on Staphylococcus aureus. Among them, the diameters of bacteriostatic zone were (20.95±0.07) mm, (23.52±0.49) mm and (23.49±0.34) mm, respectively. Lactobacillus crispatus 6-5, 6-6 and 6-7 had better inhibitory effect on Escherichia coli, the diameters of bacteriostatic zone were (19.03±0.23) mm, (18.20±0.18) mm and (18.55±0.29) mm, respectively. Conclusion:Lactobacillus crispatus 6-5 and 6-7 have good acid production, hydrogen peroxide production, bacteriostatic activity and excellent biological characteristics, so they are expected to be candidate strains for the treatment of female reproductive system diseases.

Objective:To isolate and screen Lactobacillus crispatus from healthy female vagina and evaluate its probiotic characteristics in vitro in order to provide a new strain for further screening and study of microecological agents for effective treatment of female reproductive system diseases. Methods:The vaginal secretions of 10 healthy women were collected and screened in Department of Reproductive Medicine, the Second Affiliated Hospital of Naval Medical University from April 2023 to May 2023. Lactobacillus crispatus was identified by 16S rRNA sequence, and its growth characteristics, acid-producing ability, H 2O 2 producing ability and bacteriostatic ability were detected. Results:Totally 39 strains of Lactobacillus crispatus were isolated and identified from 10 healthy women. The logarithmic growth phase of Lactobacillus crispatus was 10-40 h, among which Lactobacillus crispatus 4-5 and Lactobacillus crispatus 6-7 grew faster. The pH of Lactobacillus crispatus decreased rapidly within 8-24 h, and the pH of 48 h was stable between 3.89 and 4.05. Among them, Lactobacillus crispatus 6-6, 6-7, 6-9, 6-11 had better acid production. All 10 strains of Lactobacillus crispatus could produce H 2O 2, and Lactobacillus crispatus 4-5, 4-11, 6-5 and 6-7 had better H 2O 2 production. Lactobacillus crispatus 6-5, 6-7 and 6-9 had better inhibitory effect on Candida albicans, in which the diameters of bacteriostatic zone were (20.90±0.31) mm, (20.61±0.70) mm and (21.73±0.37) mm, respectively. Lactobacillus crispatus 4-5 and 6-7 had better inhibitory effect on Staphylococcus aureus. Among them, the diameters of bacteriostatic zone were (20.95±0.07) mm, (23.52±0.49) mm and (23.49±0.34) mm, respectively. Lactobacillus crispatus 6-5, 6-6 and 6-7 had better inhibitory effect on Escherichia coli, the diameters of bacteriostatic zone were (19.03±0.23) mm, (18.20±0.18) mm and (18.55±0.29) mm, respectively. Conclusion:Lactobacillus crispatus 6-5 and 6-7 have good acid production, hydrogen peroxide production, bacteriostatic activity and excellent biological characteristics, so they are expected to be candidate strains for the treatment of female reproductive system diseases.

Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) has spread rapidly around the world and triggered a worldwide pandemic. Whether SARS-CoV-2 potentially adversely affects female fertility is unclear now, and more evidence is needed to support the follow-up. Preliminary studies of combined SARS-CoV-2 in pregnancy have found that SARS-CoV-2 may increase the risk of preeclampsia, preterm delivery, and premature rupture of membranes, but does not appear to be associated with a high risk of early miscarriage. Whether SARS-CoV-2 infection will be vertically transmitted and affect the next generation is unclear and requires more evidence supports. With the prevalence of SARS-CoV-2, the development and clinical trials of SARS-CoV-2 vaccine are widely conducted worldwide, and current studies suggest that SARS-CoV-2 vaccination may not have adverse effects on the reproductive system of women. This article briefly reviewed the progress of research on SARS-CoV-2 on female reproductive health in the context of recent years.

Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) has spread rapidly around the world and triggered a worldwide pandemic. Whether SARS-CoV-2 potentially adversely affects female fertility is unclear now, and more evidence is needed to support the follow-up. Preliminary studies of combined SARS-CoV-2 in pregnancy have found that SARS-CoV-2 may increase the risk of preeclampsia, preterm delivery, and premature rupture of membranes, but does not appear to be associated with a high risk of early miscarriage. Whether SARS-CoV-2 infection will be vertically transmitted and affect the next generation is unclear and requires more evidence supports. With the prevalence of SARS-CoV-2, the development and clinical trials of SARS-CoV-2 vaccine are widely conducted worldwide, and current studies suggest that SARS-CoV-2 vaccination may not have adverse effects on the reproductive system of women. This article briefly reviewed the progress of research on SARS-CoV-2 on female reproductive health in the context of recent years.

The ban on addition of antibiotics in animal feed in China has made the search for new antibiotics substitutes, e.g. bacteriocin, a hot topic in research. The present study successfully isolated an antibacterial substance producing strain of Bacillus sp. from alpaca feces by agar diffusion method, using Escherichia coli, Salmonella enterica, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus and Listeria monocytogenes as indicator bacteria. The isolated strain was named as B. licheniformis SXAU06 based on colony morphology, Gram staining and 16S rRNA gene sequence. The antibacterial substance was isolated and purified through a series of procedures including (NH4)2SO4 precipitation, chloroform extraction, molecular interception and SDS-PAGE analysis. Bioinformatics analysis of the LC-MS/MS data indicated that the antibacterial substance was a bacteriocin-like substance (BLIS) with an approximate molecular weight of 14 kDa, and it was designated as BLIS_SXAU06. BLIS_SXAU06 exhibited high resistance to treatment of proteinase K, high temperature, high acidity and alkalinity. BLIS_SXAU06 was heterologously expressed in E. coli and the recombinant BLIS_SXAU06 exhibited effective antibacterial activity against S. aureus, S. epidermidis, M. luteus, and L. monocytogenes, showing potential to be investigated further.
Animals ; Anti-Bacterial Agents/pharmacology* ; Bacillus licheniformis ; Bacteriocins/pharmacology* ; China ; Chromatography, Liquid ; Escherichia coli/genetics* ; Listeria monocytogenes ; RNA, Ribosomal, 16S ; Staphylococcus aureus ; Tandem Mass Spectrometry

Objective: To evaluate the clinical effects of vitrectomy with or without fovea-sparing internal limiting membrane peeling on macular foveoschisis (MF) secondary to pathologic myopia. Methods: A non-randomized controlled study was adopted.Twenty-three patients (25 eyes) with refractive error ≥-8.00 DS and MF either combined with foveal retinal detachment or epi-macualr membrane or lamellar macular hole.The subjects were divided into non-internal limiting membrane peeling group (11 patients /11 eyes) who underwent triamcinolone (TA) assisted vitrectomy and fovea-sparing internal limiting membrane peeling group (12 patients/14 eyes) who underwent TA assisted vitrectomy with fovea-sparing internal limiting membrane peeling.The baseline data such as age, best corrected visual acuity (BCVA), refractive error, axial length were not significant difference between the two group.Main outcomes were BCVA, remission of MF defined by optical coherence tomographyc OCT as well as complications.This study followed the Declaration of Helsinki and this protocol was approved by Ethic Committee of Xiamen Eye Center of Xiamen University (NO.XMYKZX-2016-YWS-007). Results: All patients completed follow-up for more 6 months.BCVA (LogMAR) was 0.47±0.30 in non-internal limiting membrane peeling group and 0.40±0.33 in fovea-sparing internal limiting membrane peeling group, showing no significant difference between the two groups (t=0.66, P=0.52). Complete remission of MF was achieved in 22 eyes.The remission time in non-internal limiting membrane peeling group was 2.5 (1.8, 9.3) months, and 1.0 (1.0, 3.8) months in fovea-sparing internal limiting membrane peeling group, no statistical significance was obtained between the two groups (U=35.00, P=0.09). One eye had post-operative macular hole in non-internal limiting membrane peeling group, accounting for 9%, while in fovea-sparing internal limiting membrane peeling group, one eye had macular hole, accounting for 7%, and one eye had rhegmatogenous retinal detachment post-operatively, accounting for 7%. Conclusions MF can be resolved by vitrectomy while complete remission can achieved more quickly when combined with fovea-sparing internal limiting membrane peeling.

Objective: To investigate the effect of ELMOD2 over-expression on the malignant biological behaviors of gastric cancer MGC803 cells, and to study its related molecular mechanism. Methods: GV141-ELMOD2 expression vector was transfected into human gastric cancer MGC803 cells. The mRNA and protein expressions of ELMOD2 were detected by Real-time fluorescent quantitative PCR and WB, respectively. The cell proliferation ability was detected by CCK-8 method. Apoptosis rate was detected by flow cytometry. The cell migration ability was detected by Transwell method. The protein expressions of PCNA, BAX and Bcl-2 and Vimentin were detected by WB. Results:After transfection of ELMOD2 expression vector, the mRNAand protein expressions of ELMOD2 were significantly increased in MGC803 cells (P<0.05). Further studies showed that over-expression of ELMOD2 increased the proliferation and migration ability but reduced the apoptosis rate of MGC803 cells significantly (P<0.05 or P<0.01). The protein levels of PCNA, Vimentin and Bcl-2 in MGC803 cells increased, while the protein level of BAX decreased (P<0.05 or P<0.01). Conclusion: Over-expression of ELMOD2 can promote the proliferation and migration of MGC803 cells and inhibit cell apoptosis. These effects may be achieved by increasing the protein level of PCNA, Vimentin and Bcl-2, and reducing the protein level of BAX.

Objective To analyze the present situation of the dental instrument cleaning and disinfection in Hainan province and to explore the management mode of dental instrument cleaning and disinfection which is suitable for the provincial situation .Meth-ods By adopting the stratified random sampling method according to the hospital grades ,24 hospitals in Hainan province were per-formed the questionnaire survey on the cleaning mode of oral instruments ,layout of cleaning and disinfection room ,cleaning method and facilities ,protection measures and training of cleaning staff .Results 14 hospitals (58 .33% ) had the sterilization and supply center for conducting the centralized processing on the dental instruments .The tertiary hospitals and the second-grade hospitals had the independent cleaning and disinfection rooms with the rational layout and professional cleaning staff ;the safeguard facilities had the application in place ,the training of the related cleaning and disinfection work and the cleaning process conformed the require-ment of the standards .Among 10 first-grade and below hospitals ,only 1 hospital(10 .00% ) had the rational layout of cleaning and disinfection rooms ;3 hospitals(30 .00% ) had the professional cleaning staff ;the related training of the cleaning staff was not basi-cally carried out and the safeguard was not in place ,most of the cleaning and disinfection instruments and the cleaning process were not in accordance with the requirements .The qualification rates of instruments cleaning and disinfection in different grades of hospi-tals by the ATP bioluminescence assay were 100 .00% ,90 .00% and 80 .00% .Conclusion The existing problems are general and prominent in the hospitals of the first-grade and below .It is suggested that the regionalized disinfection and supply management mode is implemented for maximally realizing the optimized resource configuration in the disinfection and supply center .