Objective To explore the related risk factors for thyroid dysfunction(irTD)after immuno-therapy in patients with non-small cell lung cancer(NSCLC)and to construct a predictive model.Methods A retrospective analysis was conducted on 197 NSCLC patients who received immunotherapy at Chaohu Hospital,Anhui Medical University between January 2019 and June 2024.The patients were divided into a training set(n=137)and a validation set(n=60)in a 7∶3 ratio.Risk factors were screened through Lasso and logistic regression,and a dynamic nomogram model was constructed.The model's performance was evaluated using ROC curve,calibration curve,decision curve analysis(DCA),and clinical impact curve(CIC).Results Multivariate analysis showed that Gender(OR=0.172,95%CI:0.047～0.623),M stage(OR=2.919,95%CI:1.063～8.015),ln(SII)(OR=0.167,95%CI:0.066～0.423),ALC(OR=3.395,95%CI:1.493～7.716),and TSH(OR=1.464,95%CI:1.126～1.904)were independent risk factors for the occurrence of irTD.The model formula based on these factors is:logit(P)=9.261-1.760×Gender+1.071×M stage-1.787×ln(SII)+1.222×ALC+0.381×TSH.The model achieved an AUC of 0.832(95%CI:0.719～0.945)in the validation set,which was similar to the results in the training set.The calibration curve demonstrated good consistency between the predicted probability and actual observed values.DCA and CIC confirmed that the model has good clinical applicability.Conclusion This study identifies key risk factors for the occurrence of irTD after immunotherapy in NSCLC patients.The dynamic nomogram model developed(web calculator:https://lingchun.shinyapps.io/dynnomapp/)can effectively identify high-risk populations for irTD,providing a reliable tool for clinical decision-making.

Objective To explore the related risk factors for thyroid dysfunction(irTD)after immuno-therapy in patients with non-small cell lung cancer(NSCLC)and to construct a predictive model.Methods A retrospective analysis was conducted on 197 NSCLC patients who received immunotherapy at Chaohu Hospital,Anhui Medical University between January 2019 and June 2024.The patients were divided into a training set(n=137)and a validation set(n=60)in a 7∶3 ratio.Risk factors were screened through Lasso and logistic regression,and a dynamic nomogram model was constructed.The model's performance was evaluated using ROC curve,calibration curve,decision curve analysis(DCA),and clinical impact curve(CIC).Results Multivariate analysis showed that Gender(OR=0.172,95%CI:0.047～0.623),M stage(OR=2.919,95%CI:1.063～8.015),ln(SII)(OR=0.167,95%CI:0.066～0.423),ALC(OR=3.395,95%CI:1.493～7.716),and TSH(OR=1.464,95%CI:1.126～1.904)were independent risk factors for the occurrence of irTD.The model formula based on these factors is:logit(P)=9.261-1.760×Gender+1.071×M stage-1.787×ln(SII)+1.222×ALC+0.381×TSH.The model achieved an AUC of 0.832(95%CI:0.719～0.945)in the validation set,which was similar to the results in the training set.The calibration curve demonstrated good consistency between the predicted probability and actual observed values.DCA and CIC confirmed that the model has good clinical applicability.Conclusion This study identifies key risk factors for the occurrence of irTD after immunotherapy in NSCLC patients.The dynamic nomogram model developed(web calculator:https://lingchun.shinyapps.io/dynnomapp/)can effectively identify high-risk populations for irTD,providing a reliable tool for clinical decision-making.

Objective: To study the expression and clinical significance of Vimentin and E-cadherin in human breast cancer tissues. Methods: : The clinical data of 56 cases of breast cancer patients, who underwent radical mastectomy in Chaohu Hospital Affiliated to Anhui Medical University from January 2014 to January 2016, were retrospectively analyzed. The protein and mRNAexpressions of Vimentin and E-cadherin in breast cancer tissues were detected by immunohistochemistry and qPCR, respectively; and the relationship between the expression of Vimentin and E-cadherin in breast cancer tissues and the clinicopathological characteristics was analyzed. Logistic multivariate regression was used to analyze the independent factors affecting the protein expressions of Vimentin and E-cadherin. Spearman was used to analyze the correlation between Vimentin and E-cadherin. Kaplan-Meier was used to analyze the relationship between protein expressions of Vimentin, E-cadherin and prognosis. ROC curve was used to analyze the diagnostic value of Vimentin and E-cadherin on prognosis. Results: The rates of breast cancer tissues with high positive expression of Vimentin and E-cadherin were 76.79% and 19.64%, respectively.Among them, 47 cases (47/56, 83.93%) of breast cancer tissues showed significantly higher Vimentin mRNA expression than adjacent tissues (P<0.05), and 46 cases (46/56, 82.14%) of breast cancer tissues showed significantly lower Ecadherin mRNA expression than adjacent tissues (P<0.05). Vimentin protein expression was associated with tumor size, lymph node metastasis, vascular invasion, histological grade, clinical stage, molecular typing, Ki67+, ER-, PR- and HER2- expression (P<0.05). And E-cadherin protein expression was associated with lymph node metastasis, vascular invasion, histological grade, clinical stage, molecular typing, Ki67+, ER-, PR- and HER2- expression (P<0.05). Tumor size, lymph node metastasis, vascular invasion, histological grading, clinical staging, molecular typing, Ki67+, ER-, PR- and HER2- expression were all independent factors affecting the expression of Vimentin and E-cadherin (P<0.05). There was a negative correlation between Vimentin and E-cadherin expression (P<0.05). The 3-year survival rate of patients with high expression of Vimentin protein was 67.44%, while that of patients with low expression of E-cadherin protein was 68.89%. Conclusion: The high expression of Vimentin and low expression of E-cadherin in breast cancer tissues may be related to the occurrence, development, invasion and metastasis of breast cancer. It can be used as a reliable indicator of clinical diagnosis and prognosis.

Objective To explore the feasibility and application value of saving the left colic artery (LCA) feasibility in laparoscopic anterior resection of rectal carcinoma (Dixon).Methods Collecting the clinical data of 63 patients diagnosed by rectal cancer and underwent laparoscopic anterior resection of rectal carcinoma from January 2009 to June 2012,including 32 cases underwent saving LCA (saving LCA group) and 31 cases not saving LCA (no saving LCA group).The amount of bleeding volume,operation time,the number of lymph node dissection of inferior mesenteric artery in the operation,pass wind time,anastomotic ischemia,anastomotic leakage,and recurrence and metastasis were compared.Results No significant difference was found in the bleeding volume [(62.82 ± 19.72) ml vs.(60.68 ± 21.39) ml],operation time [(129.48 ± 13.69) min vs.(129.45 ± 19.78) min],the number of lymph node dissection of inferior mesenteric artery in the operation (2.93 ± 1.54 vs.3.21 ± 1.30) between two groups (P ＞0.05).No significant difference was found in pass wind time [(2.82 ± 1.16) d vs.(3.14 ± 0.92) d] after operation (P ＞ 0.05).The saving LCA group was not performed freeing colon splenic area and the terminal ileum stoma,the proximal intestinal blood circulation disorder and anastomotic leakage were not found.In no saving LCA group,4 cases were underwent ileostomy because of the proximal intestinal blood circulation disorder,2 cases occurred anastomotic leakage.During the follow-up of 2-24 months,1 case had a recurrence,3 cases had metastasis in saving LCA group.1 case had a recurrence,4 cases had metastasis in no saving LCA group.Conclusion Saving LCA in Dixon operation can ensure the anastomotic part with adequate blood supply,and reduce the occurrence of anastomotic leakage.

Objective To study the clinical effect of endoscopic thoracic sympathectomy at different segments on palmar hyperhidrosis and research the incidence of postoperative compensatory hyperhidrosis.Methods One hundred palmar hyperhidrosis patients who underwent endoscopic thoracic sympathectomy were divided into 2 groups according to treatment method,the patients in A group (46 cases) received T4 surgery,the patients in B group (54 cases) received T3-4 surgery.The success rate of postoperative,incidence of postoperative compensatory hyperhidrosis and postoperative satisfaction rate between the 2 groups were compared.Results The surgery of 2 groups were successful.There was no statistical difference in total effective rate between the 2 groups (P ＞ 0.05).The incidence of postoperative compensatory hyperhidrosis in A group was significantly lower than that in B group [4.3％ (2/46) vs.20.4％ (11/54)],the postoperative satisfaction rate was significantly higher than that in B group [93.5％(43/46) vs.79.6％ (43/54)],there were statistical differences (P ＜ 0.05).Conclusion Endoscopic thoracic sympathectomy T3-4 or T4 surgery is very effective and safe treatment of palmar hyperhidrosis,but T4 surgery has a lower incidence of postoperative compensatory hyperhidrosis.