OBJECTIVES: To investigate the effects of quercetin on cuproptosis and L-type calcium currents in the myocardium of diabetic rats. METHODS: Forty SD rats were randomized into control group and diabetic model groups. The rat models of diabetes mellitus (DM) induced by high-fat and high-sugar diet combined with streptozotocin (STZ) injection were further divided into DM model group, quercetin treatment group, and empagliflozin treatment group (n=10). Blood glucose and body weight were measured every other week, and cardiac function of the rats was evaluated using echocardiography. HE staining, Sirius red staining, and wheat germ agglutinin (WGA) analysis were used to observe the changes in myocardial histomorphology, and serum copper levels and myocardial FDX1 expression were detected. In cultured rat cardiomyocyte H9c2 cells with high-glucose exposure, the effects of quercetin and elesclomol, alone or in combination, on intracellular CK-MB and LDH levels and FDX1 expression were assessed, and the changes in L-type calcium currents were analyzed using patch-clamp technique. RESULTS: The diabetic rats exhibited elevated blood glucose, reduced body weight, impaired left ventricular function, increased serum copper levels and myocardial FDX1 expression, decreased L-type calcium currents, and prolonged action potential duration. Quercetin and empagliflozin treatment significantly lowered blood glucose, improved body weight, and restored cardiac function of the diabetic rats, and compared with empagliflozin, quercetin more effectively reduced serum copper levels, downregulated FDX1 expression, and enhanced myocardial L-type calcium currents in diabetic rats. In H9c2 cells, high glucose exposure significantly increased myocardial expressions of FDX1, CK-MB and LDH, which were effectively lowered by quercetin treatment; Elesclomol further elevated FDX1, CK-MB and LDH levels in the exposed cells, and these changes were not significantly affected by the application of quercetin. CONCLUSIONS Quercetin ameliorates myocardial injury in diabetic rats possibly by suppressing myocardial cuproptosis signaling and restoring L-type calcium channel activity.
Animals ; Quercetin/pharmacology* ; Calcium Channels, L-Type/metabolism* ; Diabetes Mellitus, Experimental/metabolism* ; Rats, Sprague-Dawley ; Rats ; Myocytes, Cardiac/drug effects* ; Myocardium/pathology* ; Male

Against the backdrop of rapid economic development and ongoing reforms in the healthcare system,the cover-age and quality of medical services in China are steadily improving.However,this progress is also accompanied by new challen-ges,particularly the frequent occurrence of emergencies.Cultural diversity and differences in ideological concepts serve as signifi-cant catalysts,exacerbating the complexity and unpredictability of these incidents.As a cornerstone of the healthcare system,public hospitals face particularly severe challenges in responding to emergencies.Limited by inadequate medical service levels,ethical standards,doctor-patient relationship management,and crisis response capabilities,public hospitals often encounter sub-stantial difficulties in crisis resolution.In light of this,this paper conducts an in-depth analysis of the multidimensional factors contributing to medical crises in hospitals,carefully identifying the key issues in crisis management.It proposes a series of targe-ted and actionable strategies centered around critical aspects such as crisis early warning mechanisms,accurate identification,ef-ficient response,strict control,timely feedback,and comprehensive summarization.The aim is to enhance public hospitals'abili-ty to respond to emergencies through scientific planning and effective implementation,ensuring the stability and sustainable devel-opment of healthcare services.

Objective:To establish and apply the intervention model of professional attitude for nursing undergraduates in universities of Traditional Chinese Medicine (TCM), and to explore its effect on the professional attitude of the nursing undergraduates.Methods:The study started in August 2018 included two steps. The first step was to develop intervention model of professional attitude for nursing undergraduates in universities of TCM based on the three-stage theory of Kelman ′s attitude formation and with Delphi method. After two rounds of expert consultation, the intervention model of professional attitude for nursing undergraduates in universities of TCM was finally formed, including three intervention stages, a total of 41 intervention measurements. The second step was to use the historical control research method. Grade 2016 nursing undergraduate (the year 2016—2020) in Beijing University of Chinese Medicine was taken as the experimental group ( n=86) and adopted the intervention model. Grade 2012 nursing undergraduate (the year 2012—2016) in the same university was taken as the control group ( n=56) and did not adopted the intervention model during their university study. The two groups were continuously evaluated by "Scale for Nurse ′s Professional Attitude" during their four years in the university. The measurement time was within one week of admission and the 16th week of the second semester of each academic year, with a total of 5 times of measurement. Results:Repeated measurement design showed that the scores of Scale for Nurse′s Professional Attitude of the two groups changed with time. At the end of the first year, the score of the scale in the experimental group was (6.06±0.95) points, higher than (5.41±1.08) points of the control group ( t value was 3.765, P<0.001). There was no difference between the two groups at other time points ( P>0.05). Conclusions:The intervention model of professional attitude for nursing undergraduates in universities of TCM has good operability and can improve the professional attitude of nursing undergraduates in universities of TCM during their freshman year. The intervention model needs to be further adjusted to improve the professional attitude in the subsequent learning stage.

Nursing College of Beijing University of Chinese Medicine is the first domestic research center of best practice guideline, and the first affiliated hospital-Dongzhimen Hospital is the first domestic application center of best practice guideline. The foundation of two centers sets up a high-end academic platform for better development of practice and research of evidence-based nursing. The evidence-based nursing research receives the academic guidance of international authority, and shares the international evidence-based resources. The research process can enter the NQuIRE ? database of international academic organization for unified system management. We also can communicate with international peers. The academic achievements and research achievements can be promoted and published on international platform. This essay would give a specific exposition about the evidence-based nursing and the implementation mode of best evidence.

The generic chemical structure can express succinctly and can realize large protection range, so it is widely used in chemical, pharmaceutical patents. But because of the complexity and the variety of generic chemical structure,the existing domestic patent retrieval system is unable to realize its retrieval. Efficient processing and depth of indexing for this kind of information is also the industry's problems. In order to construct one-stop service system of intellectual property, to realize the retrieval of generic chemical structure, we develop a new indexing method on the basis of the original GFL indexing language. The method further optimizes the definition of a super atom, regulates the selection principle of indexing term and the typical structure indexing to make the super atom play a greater role in the indexing and retrieval of generic chemical structure, so the method not only can improve the retrieval efficiency and convenience, but also can reduce the cost of indexing.