OBJECTIVE To investigate the effect of N-methyl-D-aspartic acid(NMDA)receptor in the secondary visual cortex(V2)on methamphetamine-associated contextual learning and memory.METHODS With male C57BL/6J mice as subjects and using the mouse conditioned place preference(CPP)experiment,the scores of CPP were observed after microinjection of NMDA receptor selective antagonist D-AP5(0.5 μg per side)into the bilateral V2 during the formation phase,single microinjec-tion of D-AP5(0.5 μg per side)into the bilateral V2 prior to the expression test,and methamphetamine(0.5 mg·kg-1,ip)-induced reactivation test and methamphetamine-associated contextual-induced reacti-vation test,to evaluate the effect of NMDA receptors on the formation,expression and reinstatement of methamphetamine-induced CPP.RESULTS Compared with the control group,microinjection of D-AP5(0.5 μg per side)into the bilateral V2 during the formation phase did not have significant inhibitory effect on CPP scores,nor did single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to the expression test,single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to metham-phetamine(0.5 mg·kg-1,ip)-induced reactivation test or single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to methamphetamine-associated contextual-induced reactivation test.CONCLU-SION The NMDA receptor in the V2 is not involved in the formation,expression and reactivation of meth-amphetamine-associated contextual learning and memory.

OBJECTIVE To investigate the effect of N-methyl-D-aspartic acid(NMDA)receptor in the secondary visual cortex(V2)on methamphetamine-associated contextual learning and memory.METHODS With male C57BL/6J mice as subjects and using the mouse conditioned place preference(CPP)experiment,the scores of CPP were observed after microinjection of NMDA receptor selective antagonist D-AP5(0.5 μg per side)into the bilateral V2 during the formation phase,single microinjec-tion of D-AP5(0.5 μg per side)into the bilateral V2 prior to the expression test,and methamphetamine(0.5 mg·kg-1,ip)-induced reactivation test and methamphetamine-associated contextual-induced reacti-vation test,to evaluate the effect of NMDA receptors on the formation,expression and reinstatement of methamphetamine-induced CPP.RESULTS Compared with the control group,microinjection of D-AP5(0.5 μg per side)into the bilateral V2 during the formation phase did not have significant inhibitory effect on CPP scores,nor did single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to the expression test,single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to metham-phetamine(0.5 mg·kg-1,ip)-induced reactivation test or single microinjection of D-AP5(0.5 μg per side)into the bilateral V2 prior to methamphetamine-associated contextual-induced reactivation test.CONCLU-SION The NMDA receptor in the V2 is not involved in the formation,expression and reactivation of meth-amphetamine-associated contextual learning and memory.

Peritoneal metastasis is a common form of distant metastasis in patients with colorectal cancer, and it is typically associated with a poor prognosis. The development of peritoneal metastasis involves complex molecular mechanisms and multifactorial regulation of the tumor microenvironment. Due to the presence of the blood-peritoneal barrier, only a small amount of systemic medication reaches the peritoneal cavity, resulting in limited efficacy against peritoneal metastasis. Intraperitoneal administration shows significant therapeutic advantages as it can directly target the tumor microenvironment, maintain high local drug concentrations, and reduce systemic toxicity. Intraperitoneal chemotherapy, especially hyperthermic intraperitoneal chemotherapy, has become a cornerstone therapeutic strategy in the clinical treatment of peritoneal metastasis. When selecting chemotherapy drugs and drug combinations, pharmacokinetic properties, efficacy, and safety must be comprehensively considered to optimize the treatment outcomes. In addition, the unique microenvironment of the peritoneal cavity provides new treatment approaches for biological treatment strategies, including antitoxins, vaccines, immune checkpoint inhibitors, etc. Techniques such as pressurized intraperitoneal aerosol chemotherapy and novel drug delivery systems demonstrate potential for enhanced efficacy, offering promising alternatives to improve patient outcomes. This article will review peritoneal barrier characteristics, intraperitoneal drug transport, intraperitoneal chemotherapy, and intraperitoneal biological therapies, thereby establishing a theoretical framework for precision therapy in colorectal cancer peritoneal metastasis.

Objective:To preliminarily evaluate the accuracy of a segmented rotational motion-based osteotomy module for autonomous dental implant robots in immediate implant placement in the anterior region through an in vitro model experiment, providing references for clinical practice. Methods:Twenty-three identical commercial maxillary resin models with dentition defects for immediate implant placement were divided into three groups: a fully guided static computer-assisted implant surgery (sCAIS) group ( n=5), a robotic computer-assisted implant surgery (rCAIS) group based on conventional coronal-apical linear motion osteotomy (rCAIS-C, n=9) and a rCAIS group based on segmented rotational motion osteotomy (rCAIS-S, n=9). Two implants were placed in each model using the corresponding surgical protocols. Intraoral scan data of pre-and post-operative models were imported into software to evaluate deviations between the actual and planned implant positions. Results:In the rCAIS-S group, the global deviations at the implant platform and apex were 0.50 (0.32) mm and 0.52 (0.22) mm, respectively, with an angular deviation of 1.08° (0.49°). These values were significantly smaller than those in the sCAIS group [platform: 0.88 (0.50) mm, apex: 1.44 (0.50) mm, angular deviation: 2.86° (1.76°), P<0.05]. rCAIS-S group also showed significantly smaller platform and apex deviations compared to rCAIS-C group [platform: 0.69 (0.12) mm, apex: 0.94 (0.16) mm, P<0.05], though angular deviations of rCAIS-S group did not differ significantly from rCAIS-C group [1.27° (0.56°), P>0.05]. Conclusions:Within the limitation of this study, the segmented rotational motion-based osteotomy module for dental implant robots demonstrates superior accuracy in immediate implant placement in the anterior area compared to sCAIS and rCAIS based on conventional coronal-apical linear motion osteotomy.

Wound healing is a significant medical challenge,particularly in the treatment of chronic wounds.Traditional therapeutic methods often fall short in addressing the complex demands of chronic wounds,such as diabetic foot ulcers and deep burns.Recently,the combination of hydrogels and stem cell technology has offered new hope for wound treatment.Hydrogels,with their excellent moisturizing properties,breathability,and biocompatibility,have become ideal materials for wound coverage.Stem cells,renowned for their regenerative and tissue repair capabilities,are highly valued in this context.Hydrogels provide a three-dimensional scaffold that mimics the wound site's natural environment,promoting the adhesion,growth,and differentiation of stem cells.They can also be engineered as delivery systems to release growth factors or drugs in a controlled manner,directly targeting the treatment area and accelerating the healing process.Meanwhile,stem cells enhance the repair functions of surrounding tissues by secreting growth factors and cytokines,improving local blood circulation,and reducing inflammation.This article reviews the development and application of hydrogels combined with stem cells in promoting wound healing,in order to provide new ideas and strategies for the clinical treatment of chronic wounds.

Peritoneal metastasis is a common form of distant metastasis in patients with colorectal cancer, and it is typically associated with a poor prognosis. The development of peritoneal metastasis involves complex molecular mechanisms and multifactorial regulation of the tumor microenvironment. Due to the presence of the blood-peritoneal barrier, only a small amount of systemic medication reaches the peritoneal cavity, resulting in limited efficacy against peritoneal metastasis. Intraperitoneal administration shows significant therapeutic advantages as it can directly target the tumor microenvironment, maintain high local drug concentrations, and reduce systemic toxicity. Intraperitoneal chemotherapy, especially hyperthermic intraperitoneal chemotherapy, has become a cornerstone therapeutic strategy in the clinical treatment of peritoneal metastasis. When selecting chemotherapy drugs and drug combinations, pharmacokinetic properties, efficacy, and safety must be comprehensively considered to optimize the treatment outcomes. In addition, the unique microenvironment of the peritoneal cavity provides new treatment approaches for biological treatment strategies, including antitoxins, vaccines, immune checkpoint inhibitors, etc. Techniques such as pressurized intraperitoneal aerosol chemotherapy and novel drug delivery systems demonstrate potential for enhanced efficacy, offering promising alternatives to improve patient outcomes. This article will review peritoneal barrier characteristics, intraperitoneal drug transport, intraperitoneal chemotherapy, and intraperitoneal biological therapies, thereby establishing a theoretical framework for precision therapy in colorectal cancer peritoneal metastasis.

Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an immune-mediated demyelinating peripheral neuropathy characterized by symmetric limb weakness, sensory disturbances, and diminished tendon reflexes. Its pathogenesis involves the interplay of T cells, B cells, and the complement system. Current first-line therapies including intravenous immunoglobulin, corticosteroids and plasma exchange exhibit limited efficacy, with approximately 30% of patients experiencing relapse or poor treatment response. In recent years, novel immunomodulatory therapies including anti-CD20 monoclonal antibodies, neonatal Fc receptor inhibitors, complement inhibitors, Bruton tyrosine kinase inhibitors, autologous hematopoietic stem cell transplantation, and chimeric antigen receptor T-cell therapy, have emerged as promising options for refractory CIDP, ushering in a new era of immunomodulatory treatment.

Objective To evaluate the application of deep learning reconstruction(DLR)in high-resolu-tion T2WI of the uterus and compare it with traditional reconstruction method.Methods A total of 45 pa-tients diagnosed with cervical cancer and undergoing pelvic MRI scans at the hospital from May to August 2024 were prospectively included in the study.DLR technology was used to reconstruct high-resolution T2WI images,which were then compared with high-resolution T2WI images obtained using traditional reconstruc-tion techniques.Likert-type scale was employed for subjective quality evaluation of artifacts and tissue con-trast in high-resolution T2WI images,while relative contrast(RC)between the lesion area and uterine myo-metrium was used for objective quality assessment of the images.Results The artifact score of high-resolu-tion T2WI images obtained using DLR technology showed no significant difference compared to traditional re-construction method(4.22±0.42 vs.4.16±0.37,P=0.18).However,the tissue contrast score was signifi-cantly higher than that of traditional reconstruction methods(4.38±0.49 vs.3.98±0.26,P＜0.001).The RC of high-resolution T2WI images obtained using DLR technology was superior to that of traditional recon-struction methods(0.74±0.06 vs.0.71±0.05,P＜0.001).Conclusion DLR demonstrates significant ad-vantages in high-resolution uterine T2WI.Although it shows no significant difference in artifact suppression compared to traditional methods,it improves tissue contrast and enhances lesion visualization.

Objective To map the super-enhancers remodeling of intestinal gastric cancer and reveal the tumor biological functions of the super-enhancers and the downstream target genes that may be activated.Methods A total of 31 normal gastric mucosal tissues,23 intestinal gastric cancer tissues and 9 intestinal gastric cancer organoids were collected from the Department of Gastroenterology of Army Medical Center of PLA from January to December 2022.Chromatin targeting histone H3K27ac modified chromatin targeting cleavage under targets and tagmentation(CUT&Tag)sequencing was conducted on above tissues.The remodeling profiles of super-enhancers in intestinal gastric cancer were analyzed and the key target genes were identified based on bioinformation tools.CRISPRi technology was used to intervene with the super-enhancers,the expression of target genes was detected with Western blotting,and the proliferation,migration and invasion abilities were detected by CCK-8 assay and Transwell chambers in the control group and the intervention group.Results There was a significant difference in the signal of super-enhancers between intestinal gastric cancer tissues and normal gastric mucosal tissues(P<0.05),and the active super-enhancers in cancer tissues may be involved in biological processes such as negative regulation of the immune system and cell adhesion.The expression of up-regulated cell migration-inducing protein(CEMIP)in tumor cells was regulated by the super-enhancers,and intervening the super-enhancers down-regulated the expression of CEMIP(P<0.05),and inhibited the cell proliferation,invasion and migration abilities of tumor cells(P<0.05).Conclusion Super-enhancer remodeling is observed in intestinal gastric cancer,and they can up-regulate the expression of CEMIP gene and promote the growth,migration and invasion of cancer cells.

Objective To identify the enhancer profile marked by histone H3K27ac modification in high-grade intraepithelial neoplasia(HGIN)in order to reveal the novel regulatory mechanism of HGIN pathogensis.Methods Gastric tissue samples were collected from Department of Gastroenterology of Army Medical Center of PLA between June 2022 and June 2023,including 14 normal gastric tissues(Nor group),31 HGIN tissues(HGIN group)and 17 gastric cancer tissues(GC group).Cleavage under targets and tagmentation(CUT&Tag)technique was employed to capture enhancer regions modified by histone H3K27ac.Multi-omics analysis was performed to identify HGIN-specific active enhancers and their potentially regulated genes.Immunohistochemical profiling was performed to assess differential expression of the gene of interest across clinically stratified specimens,combined with CRISPR-dCas9-mediated ablation of active enhancers to monitor the gene of interest transcriptional dynamics and validate enhancer-mediated regulatory mechanisms.Results Epigenomic sequencing obtained the data with excellent quality,and indicated that obvious remodeling was observed in H3K27ac enhancers in HGIN and GC groups(P<0.05),though no significant difference in the genome-wide distribution of H3K27ac modification among the 3 groups.Combining transcriptome data revealed that enhancer remodeling may up-regulate the expression of the proliferation-related target gene,CD24,in the HGIN tissue;while,inhibiting enhancer activity can notably reduce CD24 expression level(P<0.05).Immunohistochemical assay displayed a positive correlation between the expression levels of CD24 and Ki-67(P<0.001).Conclusion The remodeling of H3K27ac enhancer represents a significant epigenetic feature of the transformation from normal condition to HGIN.Remodeling of H3K27ac enhancer up-regulates CD24,which may facilitate the abnormal proliferation of gastric epithelial cells.