Objective:To evaluate the effect of general practice service based on network platform in health management of patients with multiple chronic conditions (MCC).Methods:Based on network platform, the general practitioners provided health management service for 626 MCC patients in a petrochemical company in Panjin city of Liaoning province from June 2015 to June 2016. The individualized health management plan included health counseling, diet and exercise guidance, health information delivery, health file establishment, and rational medication guidance. After intervention the patient activeness, quality of life, clinical variables were evaluated.Results:Ninety participants dropped out of study (14.38%, 90/626), the questionnaire survey was conducted in rest 536 participants. After 1-year intervention, the scores of physical functioning, role-physical, general health, vitality, social functioning, role-emotional, mental health, and total scores in SF-36 were significantly increased ( F=12.350, 13.215, 10.515, 29.114, 42.972, 10.951, 89.783 and 33.311, respectively, all P<0.05); the values of BMI, waist, systolic and diastolic blood pressure, total cholesterol, triglycerides, low density lipoprotein cholesterol, fasting blood glucose were significantly decreased( F= 30.039, 33.849, 7.152, 27.453, 59.938, 214.270, 73.488 and 62.014, respectively, all P<0.05); the values of high density lipoprotein cholesterol were markedly increased ( F=16.491, P<0.01); the overall scores of activation were increased ( F=9.471, P<0.01), the level of activation was changed (χ 2=16.183, P<0.05); but there was no change in body pain ( F=1.486, P=0.227). There was a weak correlation between patient activation and the general health ( r=0.099, P=0.013), vitality ( r=0.101, P=0.011), mental health ( r=0.099, P=0.013). Conclusion:General practice service based on network platform is feasible and effective for patients with multiple chronic conditions, after the implementation, the activation and the health outcomes are improved significantly.

Objective To characterize the expressions of androgen receptor (AR) and estrogen receptor (ER) in penis skin of patients with congenital hypospadias. Methods Dorsal prepuce, ventral prepuce, and urethral plate were harvested from 30 patients with congenital hypospadias. The expressions of AR and ER in epidermal cells and dermal fibroblasts were assessed respectively by automated immunohistochemistry. Image Pro plus 6.0 was used to analyze the optical density (OD) value of AR and ER in different parts of epidermal cells and dermal fibroblasts. Results AR and ER were located mainly in nucleis of the squamous basal cells and prickle cells, and were also found in nucleis of subcutaneous fibroblast cells. The expression of AR was lower in epidermis of urethral plate than that of dorsal prepuce and ventral prepuce (P<0.05), but no significant difference was detected in dermal fibroblasts. The expression of ER was higher in epidermis of dorsal prepuce than that of urethral plate and ventral prepuce (P<0.05). The dermal expression of ER in fibroblast cells was increased successively in dorsal prepuce, ventral prepuce and urethral plate (P<0.05). Conclusion Lower expression of AR in urethral plate may contribute the development of hypospadias. Disorder of ER in dermal fibroblast cells of prepuce may play an important role in hypospadias.

Objective To investigate the effect of hypoxia inducible factor-1ɑknocked down by small interfering RNA ( siR-NA) on cell proliferation , apoptosis and apoptotic pathways of human Y-79 RB cells.Methods Cell proliferation , HIF-1ɑmRNA and protein levels were measured by MTT , RT-PCR and Western Blot on human Y-79 RB cells under both normoxic and hypoxic conditions , respectively .siRNA knockdown against HIF-1ɑwas carried out to suppress the expression of HIF-1ɑ.Cell apotosis was determined by double staining cells with the annexin V-FITC and PI.Caspase activity was assessed by the fluorometric assay .Biostatistical analyses were conducted with SPSS 16.0 software package.Results A multifold increase in HIF-1ɑmRNA and protein levels were observed af-ter cells were exposed to the hypoxic environment at 0 h,4 h,8 h and 12 h.Both mRNA and protein levels of HIF-1ɑwere attenuated or abolished by transfection with siRNA H IF-ɑunder normoxic and hypoxic conditions .Futhermore,knockdown of HIF-1ɑcould enhance hy-poxia-induced slight increase of Bax/Bcl-2 ratio and activate caspase-9 and caspase-3.Conclusion This study was able to show that a knockdown of HIF-1ɑby siRNAH IF-1ɑresulted in a decrease in proliferation and induction of apoptosis in human Y-79 RB cells under both normoxic and hypoxic conditions .HIF-1ɑexpression may be a promising strategy for the treatment of human RB in the future .