The paper reports a 32-year-old female acute myeloid leukemia patient who developed graft-versus-host disease after paternal hematopoietic stem cell transplantation, which subsequently led to renal thrombotic microangiopathy. She subsequently required a kidney transplant from the same donor 5 years later due to renal failure. Considering that both the bone marrow and kidney were from the same donor and the recovery of renal function was favorable, immunosuppressive therapy was discontinued after a short course of anti-rejection treatment, with maintained stable kidney function. This case suggests that under the condition of high chimerism, allogeneic hematopoietic stem cell transplantation and kidney transplantation from the same donor can achieve immune tolerance, potentially improving solid organ transplantation success rate. The findings provide a novel therapeutic approach for solid organ transplantation following allogeneic hematopoietic stem cell transplantation.

The paper reports a 32-year-old female acute myeloid leukemia patient who developed graft-versus-host disease after paternal hematopoietic stem cell transplantation, which subsequently led to renal thrombotic microangiopathy. She subsequently required a kidney transplant from the same donor 5 years later due to renal failure. Considering that both the bone marrow and kidney were from the same donor and the recovery of renal function was favorable, immunosuppressive therapy was discontinued after a short course of anti-rejection treatment, with maintained stable kidney function. This case suggests that under the condition of high chimerism, allogeneic hematopoietic stem cell transplantation and kidney transplantation from the same donor can achieve immune tolerance, potentially improving solid organ transplantation success rate. The findings provide a novel therapeutic approach for solid organ transplantation following allogeneic hematopoietic stem cell transplantation.

Objective To explore the role of Fas gene expression in pathogenesis of human colonic cancer tissue.Method Expression of Fas gene protein in benign and malignant colonic tissue was detected by using flow cytometry in forty patients.Results Expression rate of Fas in normal control mucosas was(12 17?3 68)%.The expression rate in colonic cancer tissue was lower than both normal control group and benign tissue.The expression rate in inflammatory tissue was higher than the normal mucosal tissue,and the expression rate was correlated with the tumor differentiation.Conclusions Fas-mediated apoptosis may play an important role in carcinogenesis of colonic tissue.

Objective To examine deletion and mutation of p16 gene in primary pancreatic carcinoma to evaluate the relationsip between p16 gene alteration and carcinogenesis of pancreatic carcinoma as well as its progression. Methods Deletion and mutation of p16 gene in 28 primary pancreatic carcinomas and 28 normal tissues adjacent to tumors were detected by PCR-based deletion analysis technique and PCR-SSCP respectively, and the relationship between the results of detection and the clinical parameters was analysed. Results In 28 pancreatic carcinomas, homozygous deletion and mutation of p16 gene were found in 4 and 5 cases respectively. The frequency of p16 gene alteration was 32 1%. p16 gene alteration closely correlated with lymph node metastasis (P

Objective To study the effect of embolization of gastric coronary and short veins on the control of hemorrhage from gastric fundal varices. Methods Thirty-two patients with liver cirrhosis and hemorrhage from esophagus and fundal varices, who still had variceal bleeding after endoscopic banding or sclerosing of esophageal varices were included in the study. Gastric coronary and short veins were (embolized) by percutaneous, transhepatic embolization with absolute alcohol, steel ring, or gelfoam. The follow-up of 3 to 11 months was carried out after embolization. Results The follow-up endoscopy showed that the varices of gastric fundus disappeared completely in 21 of 29 patients (72.4%), and (eleminated) partially in 8 patients (27.6%). Only one patient (3.1%) had re-bleeding due to portal (hypertensive) gastropathy in the follow-up period. No obvious complication was found in these patients. Conclusions The varices of gastric fundus could be obliterated by percutaneous, transhepatic embolization of gastric coronary and short veins.

To study the expression of adhesion molecules on tissues and peripheral lymphoeytes in patients with ulcerative eolitis(UC),CD_(44),CD_(54)were assessed using flow cytometry in 34 UC patients and 20 healthy volunteers.Results showed that increments of CD_(44)on tissues and blood cells in UC were 20.3?8.2% and 19.4?6.3% respectively,that of CD_(54)being 37.2?8.9% and 18.3?8.9% as compared with the controls'(9.7?4.6%,7.8?4.5%,8.8?4.2% and 6.2?3.7% correspondingly,P

Objective To evaluate the efficacy and complication of endoscopic dense ligation for bleeding esophageal varices(EV). Methods 128 cases of EV experienced varcies ligation, and 16～22 points(average 18.8 points) of the varices were ligated by using dense ligation, while only 5～6 points were ligated in common ligation (common method) for varices. All patients got one to two times of ligation therapy. Results In dense ligation group, the rate of prompt hemostasis was 96.9%, rate of elimination of EV was 92.2% with one therapy, and 96.9% with twice therapy, while the rate of bleeding was 0.8%. In common method the rates were 95.7%, 42.6%, 64.7% and 7.1% respectively. Between the two groups, the rate of elimination of EV and rebleeding had significant difference (P

By using a micro-sensor through an endoscope,the esophageal variceal pressure in 94 patients with cirrhosis was measured.The results of the measurement were as follow:in 5 cases with Ⅱ-degree,it was 2.68?0.18 kPa,varices in 26 cases with Ⅲ-degree,it was 2.90?0.23 kPa,while in 63 cases of Ⅳ-degree varices,it was 3.51?0.33 kPa.The results suggested that there was a significant correlation between the pressure of esophageal varices with the degree of varices (r=0.313,P=0.01).The method seemed to be a valuable non-invading technic for evaluating the degree of varices.

This study was to study the effect of cyclosporine A (CsA) on the expression of nitric oxide synthase (NOS) in liver cells in T cell dependent liver injury induced by concanavalin A (ConA) in mice. The results showed that there was no NOS expression in the normal murine hepatocytes. Affer ConA challenge, the expression of NOS in hepatocytes increased markedly.Moreover, the hepatocytes closer to the central vein showed stronger expression.After pretreatment of CsA, activation of T lymphocytes was inhibited and NOS expression in the hepatocytes was almost completely inhibited too.The above results suggest that an upregulation of NOS expression might be a protective response to insufficient supply of blood and oxygen as a result of ConA induced liver injury, and inhibition of the NOS expression by CsA might be associated with inactivation of T lymphocyte.

This article reports on 217 patients with cirrhosis before sclerotherapy. It was observed that there was relationship between the 10 indexes and bleeding, i.e. the diameter of portal vein in bleeding group 1.60?0.29 cm, portal venous flow 986.5?117.8 ml/min, free portal pressure 3.76?0.28 kPa, the esophageal variceal pressure 3.56?0.25 kPa. In the patients with no bleeding, the value were 1.40?0.16 cm, 831.7?53.6 ml/min, 2.79?0.33 kPa, and 2.75?0.31 kPa, respectively. Differences between two groups were significant (P