Objective:To investigate the relationship between methylation levels of ESR1, PRKN genes and the occurrence of polycystic ovary syndrome (PCOS). Methods:In a case-control study, 60 patients with PCOS (PCOS group) and 40 women of normal reproductive age (control group) attending the Department of Reproductive Medicine, the First Hospital of Shanxi Medical University from January 2021 to June 2021 were enrolled. The peripheral blood DNA and total RNA were extracted, and the methylation specific polymerase chain reaction (MS-PCR) technique was used to detect the methylation status of ESR1 and PRKN genes promoter regions. The mRNA expression levels of ESR1 and PRKN genes were measured by real-time quantitative PCR. The relationship between the methylation status, mRNA expression levels of these two genes and the occurrence of PCOS were analyzed. Results:The methylation level of ESR1 gene in PCOS patients [56.7% (34/60)] was significantly lower than that in control group [77.5% (31/40), P=0.035]; while the methylation level of PRKN gene in PCOS patients [76.7% (46/60)] was significantly higher than that in control group [52.5% (21/40), P=0.012]. The expression level of ESR1 mRNA in PCOS patients (1.30±0.93) was significantly higher than that in control group (0.97±0.50, P=0.034); while there was no difference of PRKN expression level between PCOS (1.06±0.83) and control groups (1.15±1.01, P>0.05). Conclusion:The methylation levels in the promoter regions of ESR1 and PRKN genes lead to the changes of the mRNA expression levels, which in turn play an important role in the occurrence of PCOS.

Objective:To investigate the relationship between methylation levels of ESR1, PRKN genes and the occurrence of polycystic ovary syndrome (PCOS). Methods:In a case-control study, 60 patients with PCOS (PCOS group) and 40 women of normal reproductive age (control group) attending the Department of Reproductive Medicine, the First Hospital of Shanxi Medical University from January 2021 to June 2021 were enrolled. The peripheral blood DNA and total RNA were extracted, and the methylation specific polymerase chain reaction (MS-PCR) technique was used to detect the methylation status of ESR1 and PRKN genes promoter regions. The mRNA expression levels of ESR1 and PRKN genes were measured by real-time quantitative PCR. The relationship between the methylation status, mRNA expression levels of these two genes and the occurrence of PCOS were analyzed. Results:The methylation level of ESR1 gene in PCOS patients [56.7% (34/60)] was significantly lower than that in control group [77.5% (31/40), P=0.035]; while the methylation level of PRKN gene in PCOS patients [76.7% (46/60)] was significantly higher than that in control group [52.5% (21/40), P=0.012]. The expression level of ESR1 mRNA in PCOS patients (1.30±0.93) was significantly higher than that in control group (0.97±0.50, P=0.034); while there was no difference of PRKN expression level between PCOS (1.06±0.83) and control groups (1.15±1.01, P>0.05). Conclusion:The methylation levels in the promoter regions of ESR1 and PRKN genes lead to the changes of the mRNA expression levels, which in turn play an important role in the occurrence of PCOS.

ObjectivesTo investigate the effect of pediatric trauma life support (PTLS) curriculum on the improvement of rescue skill for pediatric trauma patients.MethodsPTLS course was introduced to train medical staff engaging in pediatric critical trauma care. The course included theoretical and practical part with main focus on enhancement of students’ decision on scene and rescue skill.ResultsCompared with pre-training, the students’ post-training written test scores improved signiifcant-ly, and the rates of qualiifcation and excellence were improved signiifcantly (P<0.01). All students were qualiifed for the tests of skill stations and simulation scenario. The excellence rates of the tests were as follows: ifxation and transportation 42.62%, basic and advanced airway management 81.97%, shock evaluation and management 70.49%, simulation scenario 72.13%. Both pre-training and post-training written test scores in hospital group were significantly higher than those in pre-hospital group (P<0.01).ConclusionsAfter PTLS courses, the knowledge, practice and integrated life-saving skills of students are greatly im-proved and teamwork is strengthened.

Objective To observe the clinical effects and nursing therapy of Jinchuangkangjungao ointment and infrared therapy in the treatment of chronic skin ulcers .Methods Seventy-five patients with chronic skin ulcers were divided into two groups :the experimental group(n=39) and the control group(n=36) .Jinchuangkangjungao ointment and infrared therapy were applied to the experimental group and the control group underwent conventional treatment ,including debridement ,dressing change and iodine wet compressing .The two group were both given basic nursing therapy .The effect and healing time were observed after 30 days . Results The experimental group′s total effective rate(92 .31% ) was significantly better than that of the control group(69 .44% ) , with significant difference(P<0 .01);The healing time of the experimental group[(11 .98 ± 6 .05)d] was significantly shorten than the control group[(16 .96 ± 7 .13)d] ,with significant difference(P<0 .05) .Conclusion Jinchuangkangjungao ointment combined with infrared therapy had noticeable effect in the treatment of chronic skin ulcers .

0.05).The costs of drug were 936.32,2 011.52 and 1 076.32 yuan respectively.CONCLUSION:Regimen of group A is same to other 2 groups in respect of therapeutic efficacy but better than them in cost of drugs.