Objective:To systematically evaluate the effect of different pressing time on bleeding in patients with subcutaneous injection of low molecular weight heparin (LMWH) .Methods:We retrieved the randomized controlled trial (RCT) or controlled clinicial trials (CCT) on local pressing time and bleeding in patients with subcutaneous injection of LMWH by searching PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI) , China Biology Medicine (CBM) , Wanfang Data and VIP from the database establishment to September 2019. Two researchers independently screened literature based on inclusion and exclusion criteria, evaluated quality and extracted data. RevMan 5.3 was used to conduct Meta-analysis on the extracted data.Results:A total of 9 studies that met the criteria were finally included and the included documents were all in Chinese, of which 4 were RCTs and 5 were CCTs. Subcutaneous hemorrhage diameter <5 mm was defined as no bleeding, subcutaneous hemorrhage ≥5 mm was defined as bleeding, and bleeding incidence was used as the final outcome indicator. According to different pressing time, they were divided into non-pressing group, group of pressing for 3 minutes, group of pressing for 5 minutes and group of pressing for 10 minutes, and were compared each group in pairs. Meta-analysis results showed there were significantly statistical differences between non-pressing group and group of pressing for 3 minutes [ OR=0.44, 95% CI (0.25, 0.78) , P=0.005], between group of pressing for 3 minutes and group of pressing for 5 minutes [ OR=2.12, 95% CI (1.18, 3.83) , P=0.010]; there were no significantly statistical differences between non-pressing group and group of pressing for 5 minutes [ OR=0.94, 95% CI (0.32, 2.81) , P=0.920], between non-pressing group and group of pressing for 10 minutes [ OR=1.11, 95% CI (0.33, 3.70) , P=0.870], between group of pressing for 5 minutes and group of pressing for 10 minutes [ OR=2.56, 95% CI (0.72, 9.11) , P=0.150]. Conclusions:Subcutaneous injection of LMWH without pressing is better than pressing for 3 minutes, and pressing for 5 minutes is better than pressing for 3 minutes. Considering the limited number of included documents and the quality grades are all B, research conclusions still need to be verified by large samples and rigorous experimental design.

The origin of precision medicine was discussed in this article,as well as its advantages and deficiencies at this stage.Tangshen formula was taken as an example to explain a rational and ideal mode of traditional Chinese medical (TCM) precision medicine research under the guidance of TCM theory.In view of the two major problems existing in the TCM modernization research,including the separated strategy among formula-disease-syndrome and independent research approach using genomics,proteomics and metabonomics,critical scientific issues on developing the drug system to biological system research mode and solutions for integrating formula-disease-syndrome were proposed here,and the roadmap and total framework of top design were also provided.The specific methods and the corresponding research results on Tangshen formula at the three stages concluded as formula-disease related,disease-syndrome combinated and formula-disease-syndrome integrated were introduced and summarized.At this time for the innovative breakthrough development of TCM,precision medicine will promote the TCM modernization research,and also will provide a powerful driving force for the establishment of holistic systems medicine.

Objective To understand the schistosomiasis epidemic situation among migrant population,so as to assess the potential risk of schistosomiasis transmission in Haining City. Methods Five districts in Haining City were randomly selected to investigate the schistosomiasis endemic situation in migrant population from the schistosomiasis endemic provinces. Results Totally 1207 persons were investigated and four positives were found in serological tests,with the positive rate of 0.33%,but no patients were found in pathologic tests. The positive cases in serological tests were mainly between 31-40 years old,with the pos-itive rate of 1.68%,which was higher than that of other age groups( χ2 = 6.590,P < 0.05). Conclusion The schistosomiasis surveillance work in migrant population still should be strengthened in Haining City.

Applications of network pharmacology are increasingly widespread and methods abound in the field of drug development and pharmacological research. In this study, we choose rosiglitazone compound as the object to predict the targets and to discuss the mechanism based on three kinds of prediction methods of network pharmacology. Comparison of the prediction result has identified that the three kinds of prediction methods had their own characteristics: targets and pathways predicted were not in accordance with each other. However, the calcium signaling pathway could be predicted in the three kinds of methods, which associated with diabetes and cognitive impairment caused by diabetes by bioinformatics analysis. The above conclusion indicates that the calcium signaling pathway is important in signal pathway regulation of rosiglitazone compound, which provides a clue to further explain the mechanism of the compound and also provides a reference for the selection and application of methods of network pharmacology in the actual research.

Major breakthrough and challenge that encountered by modern western medicine were described in this article. Some standpoints about the necessity of innovative revolution for the development of traditional Chinese medicine, together with the key points should be bring to the attention during ideological emancipation have been put forward here. Chinese scientists agreed that it is the most appropriate timing for us to establish a new medicine system, which we named Holistic Systems Medicine (HSM). The concept and research contents of Holistic Systems Medicine were also introduced in this article. Finally, a proposal for setting up“China Holistic Systems Medicine development project” was proposed.

The objective of this study is to fully investigate the therapeutic effect and mechanisms of action of Gegen Qinlian decoction (GD) on type 2 diabetes mellitus (type 2 DM). A rat model of type 2 DM was established with the combination of high-fat diet and multiple low doses of streptozotocin (STZ). Biochemical indicators related to glucose metabolism disorders, insulin resistance, oxidative stress were observed. The type 2 DM rats were administrated with GD for 80 days, the above-mentioned indexes were detected. The results indicated that the hepatic glycogen synthesis level was promoted, fasting blood glucose level and fasting blood insulin level were significantly reduced, insulin sensitivity index was significantly improved; the level of superoxide dismutase (SOD) was increased and the level of malondialdehyde (MDA) was reduced; pathologic morphology of pancreas and kidney was ameliorated in the GD group. It was indicated that the therapeutic mechanisms of action of GD on type 2 DM might be related to its effect of ameliorating glucose metabolism disorders, relieving insulin resistance, increasing the tissues' sensitivity to insulin, improving the antioxidative ability of living system, GD has therapeutic effect on type 2 DM and protective effects against damaged pancreatic function.

This study was to report the effect of Tangshen Formula on phospholipids metabolism in diabetic nephropathy patients. A normal phase-HPLC-TOF/MS method was used in this study for the determination of seven species of phospholipids in human plasma. Then, the concentration changes of potential phospholipids biomarkers were discussed in diabetic nephropathy phase III and phase IV patients among different groups, including before and 3, 6 months after administration of Tangshen Formula. Significant increases of PE750, PI885, PC792, PC826, PC830, PC854 and PC802 levels were observed 6 months after administration of Tangshen Formula and conventional western medicine, as well as a decrease of LPC540 level, when compared with those before medication. Concentrations of all the potential phospholipids biomarkers showed a tendency towards normal levels; however, both the improvement degree and onset time of these compounds were not same. Additionally, Tangshen Formula treatment based on conventional western medicine treatment was more efficient in adjusting the levels of these compounds when compared with western medicine treatment alone, especially for the phase IV patients. These results indicated that Tangshen Formula was capable in regulating and improving phospholipids metabolism in diabetic nephropathy patients, which may be related with the direct or indirect inhibition of protein kinase C pathway and the corresponding reduction of phospholipase A2 activity. Therefore, Tangshen Formula may be used as an effective drug for diabetic nephropathy therapy, at least as an adjunctive therapeutic drug.

OBJECTIVETo verify the rational of Chinese medicine Shuanglong formula.

METHODRat models of acute myocardial ischemia were induced by hydrochloride isoproterenol. ECG J point change, myocardial infarction area and cellular enzyme (CK, LDH, SOD and MDA) levels were observed and detected to show protective effect of treatment groups.

RESULTThe single drug prescriptions and compatibilities both could improve ECG performance, decrease levels of CK, LDH, SOD and MDA in serum, and reduce a certain myocardial infarct size.

CONCLUSIONThe Shuanglong formula at the high dose of 3:7 was proved to be more effective on myocardial ischemia.

Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Electrocardiography ; drug effects ; Isoproterenol ; Male ; Myocardial Infarction ; drug therapy ; Rats ; Rats, Sprague-Dawley

Objective To explore the efficacy and value of the one-off cure of the serious cervical erosion by Microwave combined with idophor.Methods Eighty patients with serious cervical erosion enrolled in our department from 8,2003 to 10,2005 were randomly allocated to group A as test group and group B as control group.Both groups were treated with microwave.But every one or three days, cervix and vagina of group A was scrubbed by idophor.After three months,every group was re - examined to observe the efficacy and side -effect of the one-off cure.Results The one-off cure rate in group A(95%)was significantly higher than in group B(77.5%). There was a significant difference between the two groups(P＜0.05).The average days of took off scab in group A(9.66)had a signifi- cantly deeper invasion than in group B(13.87)(P＜0.05).Conclusion On cure of the serious cervical erosion by microwave combined with idophor can shorten the took off scab period and improved the one - off cure rate.The convenient method can be used widely.

0.999). The relative standard deviation (RSD) of chromatogram area was less than 1.0% for all of them after six successive injections. The detection limit was 0.05 ?g/mL for norephedrine (NE), 0.04 ?g/mL for norpseudoephedrine (NPE), 0.1 ?g/mL for E and PE, and 0.2 ?g/mL for mephedrine (ME) and mepseudoephedrine (MPE), respectively (S/N≥3). The recovery rate was more than 97.1% for six ephedrine alkaloids. Under this method system, all of the above-mentioned six samples were tested and found to contain some of the impurity at different levels. Conclusion This developed method, which is very simple, perfect precision, high sensitivity, and selectivity, can be used for the qualitative and quantitative determination of impurities of ephedrine alkaloid-type samples.