Objective To investigate the significance of CD4 and CD8 T lymphocyte ATP release assays for the assessment of cellular immune function in patients with digestive tract tumors.Methods 51 digestive tract tumor patients from the Department of Oncology and Department of Radiotherapy of the General Hospital of Southern Theater Command of PLA during 2023～2024 as a study group,and 51 healthy medical checkups were collected from the Physical Examination Center of that Hospital during 2018～2020 as a control group,and the counts of leukocytes,lymphocytes,CD4 T lymphocyte,and CD8 T lymphocyte were conducted to detect the release of ATP from CD4,CD8 T lymphocyte before and after the stimulation of PHA Levels of ATP release from CD4 and CD8 T lymphocytes before and after PHA stimulation.Results Lymphocyte counts[1.45(1.12,1.75)×109/L]and CD8 T lymphocyte counts[357.00(232.13,451.35)个/μl]in the research group was lower than in the control group[2.04(1.24,2.27)×109/L,454.36(368.00,566.10)个/μl],and the differences were statistically significant(Z=-4.719,-3.209,all P<0.05).After PHA stimulation,the ATP release level of CD4 T lymphocytes in the research group was lower than that in the control group(Z=-2.181,P＜0.05),and the level of ATP release level of CD8 T lymphocytes in the research group before and after PHA stimulation were lower than those in the control group(Z=-3.314,-3.462,all P<0.05).There was no correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the research group(r=0.617,P=0.093),and there was a positive correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the control group(r=0.276,P=0.049).There was no significant correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the research group(r=0.033,P=0.816),and a positive correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the control group(r=0.278,P=0.048).Conclusion The CD4 and CD8 T lymphocyte ATP release assay can be used as a method to evaluate the dynamic function of cellular immunity,and in combination with static cell counting can provide a more comprehensive assessment of cellular immunity in patients with gastrointestinal tumors.

Objective To investigate the significance of CD4 and CD8 T lymphocyte ATP release assays for the assessment of cellular immune function in patients with digestive tract tumors.Methods 51 digestive tract tumor patients from the Department of Oncology and Department of Radiotherapy of the General Hospital of Southern Theater Command of PLA during 2023～2024 as a study group,and 51 healthy medical checkups were collected from the Physical Examination Center of that Hospital during 2018～2020 as a control group,and the counts of leukocytes,lymphocytes,CD4 T lymphocyte,and CD8 T lymphocyte were conducted to detect the release of ATP from CD4,CD8 T lymphocyte before and after the stimulation of PHA Levels of ATP release from CD4 and CD8 T lymphocytes before and after PHA stimulation.Results Lymphocyte counts[1.45(1.12,1.75)×109/L]and CD8 T lymphocyte counts[357.00(232.13,451.35)个/μl]in the research group was lower than in the control group[2.04(1.24,2.27)×109/L,454.36(368.00,566.10)个/μl],and the differences were statistically significant(Z=-4.719,-3.209,all P<0.05).After PHA stimulation,the ATP release level of CD4 T lymphocytes in the research group was lower than that in the control group(Z=-2.181,P＜0.05),and the level of ATP release level of CD8 T lymphocytes in the research group before and after PHA stimulation were lower than those in the control group(Z=-3.314,-3.462,all P<0.05).There was no correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the research group(r=0.617,P=0.093),and there was a positive correlation between CD4 T lymphocyte ATP release values and CD4 T lymphocyte counts in the control group(r=0.276,P=0.049).There was no significant correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the research group(r=0.033,P=0.816),and a positive correlation between the ATP release values of CD8 T lymphocytes and lymphatic counts in the control group(r=0.278,P=0.048).Conclusion The CD4 and CD8 T lymphocyte ATP release assay can be used as a method to evaluate the dynamic function of cellular immunity,and in combination with static cell counting can provide a more comprehensive assessment of cellular immunity in patients with gastrointestinal tumors.

Objective:To explore the effects and mechanism of anti IL-9 antibody on malignant ascites ( MA) of hepatic carci-noma in mice.Methods:A mouse model of MA was established by mouse H 22 cell line.45 mice were divided randomly into experi-mental group,negative control group and blank control group at 24 hours after modeling,with 15 mice in each group.The experimental group was injected intraperitoneally with anti IL-9 antibody;the negative control group was injected with isotype IgG antibody;the blank control group was injected with normal saline .The weight and behavior of the mice were measured before each injection .Five mice of each group was sacrificed at 24 hours after the last injection to measure the volume of MA .The level of VEGF,MMP-2,IL-9 and IFN-γin MA were determined with ELISA assay;the survival time of rest mice were recorded and compared .Results:The mean volume of MA of experimental group,negative control group and blank control group were (6.70±1.52)ml,(10.28±1.75)ml,(10.36±2.30) ml,respectively,the MA volume of experimental group were lower as compared to negative control group and blank control group ( P<0.05).The mean survival time of experimental group was (17.2±2.1)d,which was significantly prolonged compared with the negative control group (14.5±1.2)d and the blank control group (14.8±1.4)d (P<0.05).The levels of VEGF of MA in experimental group was significantly lower compared to the negative control group and blank control group (P<0.05).The levels of IL-9 of MA in experi-mental group was significantly lower compared to the negative control group (P<0.05).The levels of MMP-2 and IFN-γin experimental group had no significant difference compared with the negative control group and blank control group ( P>0.05 ) .Conclusion:Intraper-itoneal injection anti IL-9 antibody on H22 ascites-bearing mice can effectively inhibit the generation of the MA .The mechanism may be related to the inhibition of the expression of the VEGF and IL-9.

0. 05). Conclusions Panipenem and betamipron are effective and safe drugs in the treatment of serious in-hospital infection in elderly patients.