Objective To compare postoperative major adverse events in patients with triple-vessel disease undergoing complete versus incomplete revascularization during coronary artery bypass grafting (CABG). Methods A retrospective analysis was conducted on patient data from Tianjin Chest Hospital who underwent primary isolated CABG surgery between 2019 and 2020. Patients were divided into a complete revascularization group and an incomplete revascularization group based on the revascularization status. Inverse probability of treatment weighting (IPTW) was used for risk adjustment. Results A total of 1 419 patients were included in the study, with 1 086 (76.5%) undergoing complete revascularization. IPTW analysis showed that complete revascularization could reduce the incidence of major adverse cardiovascular and cerebrovascular events [HR=0.596, 95%CI (0.404, 0.880), P=0.010] and angina [HR=0.560, 95%CI (0.377, 0.823), P=0.004]. Conclusion In patients with multivessel coronary artery disease, complete revascularization may be associated with improved patient outcomes.

Objective: To analyze the spatial-temporal clustering characteristics of pulmonary tuberculosis (PTB) in Zhangjiajie City, Hunan Province from 2016 to 2024, so as to provide a basis for formulating regional PTB prevention and control strategies. Methods: Data of reported PTB cases in Zhangjiajie City from 2016 to 2024 were collected from the Tuberculosis Surveillance System of China Disease Prevention and Control Information System, and the reported incidence of PTB was analyzed. Joinpoint regression model was used to analyze the trend in reported incidence of PTB from 2016 to 2024. With township (subdistrict) as the basic spatial unit, global and local spatial autocorrelation and hotspot analysis were used to analyze the spatial distribution characteristics of PTB, and spatial-temporal scan analysis was used to identify spatial-temporal clustering characteristics. Results: A total of 14 176 PTB cases were reported in Zhangjiajie City from 2016 to 2024, with an average annual reported incidence of 104.42/105, showing an overall downward trend (AAPC=-5.005%, P<0.05). Global spatial autocorrelation analysis showed that except for 2021-2022, the reported incidence of PTB showed positive spatial correlation in other years, with Moran's I values ranging from 0.134 to 0.306 (all P<0.05). Local spatial autocorrelation analysis and hotspot analysis showed that high-high clusters and hotspot areas from 2016 to 2024 were concentrated in Badagongshan Town, Longtanping Town, Chenjiahe Town, Shanghexi Township, and Liaojiacun Township of Sangzhi County, and Yinjiaxi Township and Luoshui Township of Yongding District. Spatial-temporal scan analysis showed that Class Ⅰ cluster was located in the northwest, centered on Badagongshan Town, covering 13 townships in Sangzhi County, with clustering period spanning from 2017 to 2020. The Class Ⅱ cluster was located in the southwest, centered on Maoyanhe Town, covering 7 townships in Yongding District, with clustering period spanning from 2020 to 2023. Conclusions The reported incidence of PTB in Zhangjiajie City from 2016 to 2024 showed an overall downward trend. The spatial-temporal clusters shifted from the northwest of Sangzhi County to the southwest of Yongding District.

Meat food is an important part of people's dietary supplements,but in recent years,meat adulteration cases are hidden and frequent,which seriously affects people's health and destroys the fairness of the market.Based on this,this paper analyses the development trend of meat adulteration and the current meat adulteration detection technology is summarized,combed in detail the use of the most widely used real-time fluorescence quantitative PCR technology application of the status quo and challenges to help real-time fluorescence quantitative PCR technology testing in meat adulteration evidence testing to the direction of more high detection efficiency and higher accuracy development.

Meat food is an important part of people's dietary supplements,but in recent years,meat adulteration cases are hidden and frequent,which seriously affects people's health and destroys the fairness of the market.Based on this,this paper analyses the development trend of meat adulteration and the current meat adulteration detection technology is summarized,combed in detail the use of the most widely used real-time fluorescence quantitative PCR technology application of the status quo and challenges to help real-time fluorescence quantitative PCR technology testing in meat adulteration evidence testing to the direction of more high detection efficiency and higher accuracy development.

Objective To construct recombinant human butyrylcholinesterase(rhBChE)knock-in goat fetal fibroblast cell lines(GFFs)by using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated homology-directed repair mechanism for subsequent production of goat expressing rhBChE.Methods The efficient sgRNA sites targeting goat[3-casein(CSN2)gene were designed and screened,and the targeting efficiency of the sgRNA in goat mammary epithelial cells(GMECs)was confirmed by electro-transfection,flow sorting,and sequencing of PCR products.The red fluorescent reporter gene homology repair vector(P2A-mCherry)targeting the sgRNA was constructed,and then the integration and expression efficiency was detected by flow cytometry.The rhBChE homology repair vector(P2A-rhBChE)targeting the sgRNA of CSN2 gene was constructed in GFFs,the rhBChE positive cell clones were obtained via electro-transfection and flow sorting,and the rhBChE knock-in cell lines was identified by sequencing of PCR products.Results The sgRNA4 was identified as an efficient target of goat CSN2 gene,which could be also used for targeted integration of other genes.Three rhBChE knock-in cell lines were successfully constructed.Conclusion The rhBChE knock-in GFFs targeting goat CSN2 gene lays the foundation for the production of mammary bioreactors expressing rhBChE.

Objective:To analyze the clinical features, treatment, pathology and prognosis of retroperitoneal paraganglioma(PGL).Methods:Surgery adopted, pathology and follow-up data of patients with retroperitoneal PGL at the First Affiliated Hospital of Zhengzhou University from Jan 2015 to Jan 2022 were retrospectively analyzed.Results:Compared with non-functional PGL patients, those with functional PGL had higher systolic and diastolic blood pressure (180 mmHg vs. 140 mmHg, Z=-4.807, P<0.001;100 mmHg vs. 82 mmHg, Z=-4.495, P<0.001)at admission, and were more prone to hemodynamic instability during operation ( χ2=8.188, P=0.004). All 65 patients under wentresection,with partial excision and repair of inferior vena cava in 1 patient . Sixty-two patients out of 65 were followed up, and 4 patients died of disease progression. The overall 5-year survival rate was 92%. The prognosis of patients with G3 tumor and distant metastasis was poor , the difference was statistically significant ( χ2=4.259, P=0.039; χ2=13.061, P<0.001). Tumor diameter and tumor functional status were not related to the prognosis, and the difference was not statistically significant ( χ2=0.519, P=0.472; χ2=0.010 P=0.920). Conclusions:Retroperitoneal PGL is less common, and some may encroach abdominal large vessels. The prognosis is good after complete resection of the tumor. Distant metastasis and G3 tumors are associated with poor prognosis .

Objective:To investigate the efficacy and safety of prolonged dual antiplatelet therapy (DAPT) (aspirin + clopidogrel) after coronary artery bypass grafting (CABG) for more than 12 months.Methods:1 900 patients who received CABG treatment in Tianjin Chest Hospital from January 2019 to October 2020 were continuously included, and 1 528 patients were finally identified according to the inclusion and exclusion criteria. According to whether the patients continued to take DAPT treatment 12 months after discharge, they were divided into the extended DAPT group and the standard DAPT group. Cox multivariate regression and propensity score matching (PSM) analysis were performed on major cardiovascular and cerebrovascular adverse events (MACCE) and clinically related bleeding events in the two groups during 12-24 months after discharge to evaluate the efficacy and safety of extended DAPT treatment for more than 12 months. Results:Of the 1 528 patients, 624 (40.8%) continued to take DAPT 12 months after discharge. Compared with patients receiving standard DAPT, patients receiving extended DAPT had a lower incidence of MACCE within 12 to 24 months ( HR=0.597, 95% CI: 0.399-0.892, P=0.012); ( HR=0.519, 95% CI: 0.338-0.798, P=0.003), and there was no significant increase in clinically relevant bleeding risk ( HR=1.209, 95% CI: 0.522-2.798, P=0.658), ( HR=1.112, 95% CI: 0.452-2.737, P=0.817). At the same time, prolonged DAPT treatment also brought a good net benefit. Conclusion:Prolonged DAPT treatment after CABG for more than 12 months significantly reduced the risk of ischemia at 12-24 months after surgery, and did not significantly increase the risk of bleeding at 12-24 months after surgery. It may be beneficial for patients treated with CABG to continue DAPT (aspirin+ clopidogrel) on the basis of intensive DAPT therapy for 1 year.

BACKGROUND: In clinical Ia (cT1N0M0) patients, some may have poor prognosis, for it might occur pathologic N1 (pN1) or N2 (pN2) postoperatively. The aim of this study is to determine the radiologicaland pathological factors related to clinical Ia adenocarcinoma. METHODS: The retrospective study was conducted on 297 clinical Ia adenocarcinoma patients resected at our hospital between May 2012 to December 2016. The clinical profiles, radiological and pathological features were analyzed between nodal upstaging group and non-upstaging group. RESULTS: Of 297 patients treated for cN0 tumors, 250 cases (84.2%) were confirmed postoperatively as having pN0 tumors, and 47 (15.8%) were confirmed as having pN1 or pN2 tumors. Female, low smoking index, micropapillary predominant and solid predominant adenocarcinoma, puresolid tumor and large tumor size were all more frequently seen in the nodal upstaging group than in the pN0 group (P<0.05). Logistic regression indicate that radiological solid tumor, micropapillary predominant and solid predominant adenocarcinoma and vessel invasionare the risk factors of nodal upstaging in clinical Ia adenocarcinoma. CONCLUSIONS Radiological solid tumors, micropapillary predominant and solid predominant adenocarcinoma andvessel invasion are risk factors for nodal upstaging for early stage lung cancer. Radiological solid tumors should perform SLND in Ia adenocarcinomas.
Adenocarcinoma ; diagnosis ; pathology ; Adenocarcinoma of Lung ; Female ; Humans ; Lung Neoplasms ; diagnosis ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Retrospective Studies ; Risk Factors

Objective To explore the therapeutic effect of K8 short wave therapeutic apparatus on facial sensitive skin.Methods 130 patients with facial sensitive skin admitted in our hospital from November 2015 to September 2017 were selected as the subjects.According to the different surgical methods,65 cases in the control group were treated with intensive care cream.On the basis of the control group,65 cases in observation group were given K8 therapeutic apparatus.Then we compared the treatment effect between the two groups.Results K8 shortwave therapeutic apparatus to treat facial sensitive skin can achieve higher total effective rate,reduce the skin area score and and erythema parameters,reduce the skin water loss and improve skin stratum corneum moisture content.After two weeks of treatment,the total effective rate of the observation group (98.46％) was higher than that of the control group (86.15％),the skin area score [(2.28 ± 0.26) piont],the erythema parameter (180.28 ± 29.87) and the epidermis water loss [(9.85 ±2.25)g/(cm2 ×h)] were lower than that in the control group [(4.54 ± 1.14) point,223.87 ± 30.47,(13.02 ± 3.25) g/(cm2 × h)],while the water content of the skin cuticle [(88.75 ± 10.35) ％] was higher than that of the control group [(78.98 ± 9.58) ％] (all P ＜ 0.01).Conclusions K8 short-wave therapeutic apparatus combined with intensive care cream has a better clinical effect and certain advantage in the treatment of facial skin sensitive skin than the use of intensive care cream alone.

Objective To explore the effect of prehospital 12-lead electrocardiogram (ECG) and transtelephonic notification on door-to-balloon time in patients with ST-segment elevation myocardial infarction (STEMI). Methods Four hundred and two patients with STEMI who underwent primary PCI from January 2006 to December 2007 in Beijng Anzhen Hospital were analyzed. They were divided into 3 groups: 137 patients without prehospital ECG (group A), 176 patients with prehospital ECG (group B) and 89 patients with prehospital ECG and early transtelephonie notification (group C). Door-to-balloon time and in-hospital mortality were compared among the groups. Results There were no significant differences in age, sex, past medical history and infarcted area among three groups. Compared with group A, patients in group B and group C had much shorter door-to-balloon time (96 minutes and 86 minutes vs. 113 minntes in group A, all P<0. 01). No difference was found in in-hospital mortality among three groups (2. 9% vs. 2.3% vs. 2. 2%, P> 0. 05). Conclusions Prehospital ECG and early transtelephonic notification can significantly shorten door-to-balloon time in patients with STEMI. Coordinated system including prehospital ECG and transtelephonie notification is proved to be feasible and effective.