Rationale and Background:: Antimicrobial misuse and resistance are significant global health concerns. Parents play a crucial role in administering medications to their children, making their knowledge, attitudes, and practices (KAP) essential in the fight against antimicrobial resistance. Objectives: : This study aimed to assess the knowledge, attitudes, and practices of parents regarding antibiotic use in Vientiane Capital, Lao People’s Democratic Republic. Methodology: : Data were collected using a self-administered questionnaire. Descriptive statistics, including percentages, means, and standard deviations, were used for data analysis. Results: : The study found that 51.5% of parents had moderate knowledge regarding antibiotic use. However, attitudes towards antibiotic use were generally negative, and 90% of parents exhibited poor practices related to antibiotic use in children. Conclusion: Given the moderate knowledge level, negative attitudes, and poor practices observed, efforts should be made to improve parental knowledge, attitudes, and responsible antibiotic use behaviors to help combat antimicrobial resistance.