Objective To suggest countermeasures by analyzing the aviation effects on pilots' lymphocyte and its subsets in peripheral blood and to provide basis for taking active counter measures and managing their immune function of lymphocyte.Methods We sampled the peripheral blood from 51 male fighter pilots (pilot group),who were hospitalized for physical examination,and 25 healthy male military personnel (control group) to detect the surface markers of CD45+ lymphocytes,CD3+T lymphocytes,CD4+T lymphocytes,CD8+T lymphocytes,native T lyrnphocytes,memory T lymphocytes and regulatory T lymphocytes in peripheral blood by flow cytometry.The pilot group was further divided into other subgroups by flying hours:＜1 000 h,1 000-2 000 h and ＞2 000 h.The detect results were compared between pilot and control groups and among flying hour groups.Results As compared with the control group,the mean values of surface marker of CD45+ lymphocyte and CD8+ T lymphocytes in peripheral blood of pilot group were significantly higher (0.365±0.074 vs.0.308±0.084,0.370±0.075 vs.0.320+0.071).The comparison showed statistical differences (t=3.051,2.752,P＜0.01).The CD4+/CD8+ ratio of pilot group was significantly lower than that of control group (1.34±0.54 vs.2.04±1.54,t=2.928,P＜0.01).Other comparisons had no significant difference between two groups.The mean value of CD4+/CD8+ ratio,the mean values of the surface marker of CD8+ native T lymphocytes and CD8+ memory T lymphocytes in peripheral blood showed significances among different flying hour groups (P＞0.05),but the mean values of surface marker CD45+ lymphocytes,CD3+ T lymphocytes,CD4+ T lymphocytes,CD8+ T iymphocytes,CD4+ native T lymphocytes,CD4+ memory T lymphocytes and regulatory T lymphocytes had no significant difference(P＞0.05).Conclusions There are statistical differences on the mean values of surface marker of CD45+ lymphocytes,CD8+ T lymphocytes in peripheral blood and CD4+/CD8+ ratio between pilot group and control group.The mean values of CD4+/CD8+ ratio and surface marker of CD8+ memory T lymphocytes,CD8+ native T lymphocytes show differences among different hour groups.Those indicate certain immune disorder in the fighter pilots.It may relate to the flying environment factors.The corresponding protection is suggested.

Objective To suggest countermeasures by analyzing the aviation effects on pilots' lymphocyte and its subsets in peripheral blood and to provide basis for taking active counter measures and managing their immune function of lymphocyte.Methods We sampled the peripheral blood from 51 male fighter pilots (pilot group),who were hospitalized for physical examination,and 25 healthy male military personnel (control group) to detect the surface markers of CD45+ lymphocytes,CD3+T lymphocytes,CD4+T lymphocytes,CD8+T lymphocytes,native T lyrnphocytes,memory T lymphocytes and regulatory T lymphocytes in peripheral blood by flow cytometry.The pilot group was further divided into other subgroups by flying hours:＜1 000 h,1 000-2 000 h and ＞2 000 h.The detect results were compared between pilot and control groups and among flying hour groups.Results As compared with the control group,the mean values of surface marker of CD45+ lymphocyte and CD8+ T lymphocytes in peripheral blood of pilot group were significantly higher (0.365±0.074 vs.0.308±0.084,0.370±0.075 vs.0.320+0.071).The comparison showed statistical differences (t=3.051,2.752,P＜0.01).The CD4+/CD8+ ratio of pilot group was significantly lower than that of control group (1.34±0.54 vs.2.04±1.54,t=2.928,P＜0.01).Other comparisons had no significant difference between two groups.The mean value of CD4+/CD8+ ratio,the mean values of the surface marker of CD8+ native T lymphocytes and CD8+ memory T lymphocytes in peripheral blood showed significances among different flying hour groups (P＞0.05),but the mean values of surface marker CD45+ lymphocytes,CD3+ T lymphocytes,CD4+ T lymphocytes,CD8+ T iymphocytes,CD4+ native T lymphocytes,CD4+ memory T lymphocytes and regulatory T lymphocytes had no significant difference(P＞0.05).Conclusions There are statistical differences on the mean values of surface marker of CD45+ lymphocytes,CD8+ T lymphocytes in peripheral blood and CD4+/CD8+ ratio between pilot group and control group.The mean values of CD4+/CD8+ ratio and surface marker of CD8+ memory T lymphocytes,CD8+ native T lymphocytes show differences among different hour groups.Those indicate certain immune disorder in the fighter pilots.It may relate to the flying environment factors.The corresponding protection is suggested.

AIM:To investigate the role of anti-miR-21 oligonucleotide ( AMO) in the anti-leukemic activity of decitabine (DCA) in vitro.METHODS:AMO and scramble oligonucleotide (SCR) were constructed and transfected into HL-60 cells.The miR-21 expression was analyzed by real-time PCR to identify the transfection efficiency .The cells were treated with DCA at gradient concentrations (0.5, 2.0 and 4.0 μmol/L) for 48 h.The mRNA expression of human period circadian protein 3 (hPer3) was detected by real-time PCR.The early apoptotic rates were determined by flow cy-tometry with Annexin V/PI staining.Mean fluorescence intensities ( MFI) of CD117 and CD11b were also measured by flow cytometry.RESULTS:The miR-21 relative expression level in AMO group was significantly lower than that in blank group and SCR group (P<0.01).IC50 of DCA in AMO group was significantly lower than that in blank group and SCR group (P<0.01).With the same concentration of DCA, the early apoptotic rate, the mRNA expression of hPer3 and the MFI of CD11b in AMO group were significantly higher than those in blank group and SCR group (P<0.01).The MFI of CD117 in AMO group were significantly lower than those in blank group and SCR group ( P<0.01 ) .CONCLUSION:Activation of hPer3 expression plays an important role in enhanced anti-leukemic activity of decitabine by AMO in vitro.