Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective:To investigate the clinical value of endometrial receptivity test(ERT)combined with per-sonalized embryo transfer in improving pregnancy outcomes in patients with repeated implantation failures.Meth-ods:The clinical data of patients diagnosed with repeated implantation failure at the Reproductive Medicine Cen-ter,The Sixth Affiliated Hospital of Sun Yat-Sen University from March 2019 to August 2022 were retrospectively analyzed,among which 428 patients who underwent endometrial receptivity test were included in the ERT group.396 patients who did not undergo ERT were selected by propensity score matching of SPSS 26.0 software as the control group.We compared the clinical characteristic and pregnancy outcomes of two groups and ana-lyzed the pregnancy outcomes of individualized embryo transfer in patients with normal,delayed by 1 day and de-layed by 2 days of endometrial window of implantation(WOI)after personalized embryo implantation of shifting WOI patients.Results:The clinical pregnancy rate(47.73％vs.12.79％)and the live birth rate(38.97％vs.9.76％)of ERT group were significantly higher than those in the control group(P＜0.05).Moreover,endome-triosis and polycystic ovary syndrome(PCOS)accounted for a higher proportion of infertility factors in the ERT group compared to the control group(P＜0.05).Among 428 patients who underwent ERT,there were 316 pa-tients with normal WOI(73.83％),112 patients with WOI displaced(26.17％).There was no statistical difference in the pregnancy outcome among the three groups of normal WOI delayed by 1 day and WOI delayed by 2 days(P＞0.05).Conclusions:ERT technology combined with personalized embryo transfer protocol can improve the fertility outcomes of RIF patients,especially for those with WOI displacement.ERT technology has application prospect and clinical application value.

Objective To investigate the role of nuclear factor-κB(NF-κB)/NOD-like receptors family pyrin domain containing 3(NLRP3)pathway in limb ischemia-reperfusion injury(IRI)pretreated with ulinastatin(UTI).Methods Twenty-one SD rats were randomly divided into Sham group,IRI group,UTI group.In UTI group,ulinastatin was injected after anesthesia.After 10 minutes,the animal model was established by claming the femoral artery and ligaturing collateral circulation.After 3h of ischemia,the clamp and tourniquet were removed and the rats underwent 2h of reperfusion.In the other groups,the homologous saline was also injected in the same time.Plasma concentrations of lactate dehydrogenase(LDH)and creatine kinase(CK),interleukin(IL)-6,IL-18,tumor necrosis factor-α(TNF-α)were measured.The gastrocnemius muscle was harvested and immediately stored at-80℃.NF-κB and NLRP3 were detected by Western blot and PCR.The other section muscle was stored in triformol for HE staining.The wet/dry was also immediately detecting.Results The level of wet/dry,LDH,CK,IL-6,IL-18,TNF-α,NF-κB,NLRP3 IRI were higher than those in Sham group(P＜0.05).The level of wet/dry,LDH,CK,IL-6,IL-18,TNF-α,NF-κB,NLRP3 IRI were significantly lower than those in IRI group(P＜0.05).Conclusion Ulinastatin can reduce the cellular inflammatory response through the NF-κB/NLRP3 pathway,thereby achieving a protective effect on limb IRI in rats.

Objective To investigate the role of low dose esketamine in pretreated limb ischemia-reperfusion inju-ry.Methods The rats were divided into sham-operated group(Sham group),ischemia-reperfusion group(I/R group,3 h of ischemia and 2 h of reperfusion),esketamine group(ESK group,ip,5 mg/kg).The plasma con-centrations of creatine kinase(CK)and lactate dehydrogenase(LDH)were measured.The wet/dry weight ratio of skeletal muscle was immediately detected.The gastrocnemius muscle was harvested and the level of malondial-dehyde(MDA)and superoxide dismutase(SOD)was detected by colorimetric assay.The expression of nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)were detected by Western blot and im-munohistochemical staining.Results Compared with Sham group,the index of wet/dry weight ratio,MDA,CK,LDH,Nrf2 and HO-1 were all increased,but SOD was decreased in I/R group(P＜0.05).Compared with I/R group,the index of wet/dry weight ratio,MDA,CK and LDH were significantly lower,but SOD and Nrf2 and HO-1 were significantly higher in ESK group(P＜0.05).Conclusions ESK may increase Nrf2 in the nucleus,thereby increase the HO-1 protein as an antioxidant agent.