Background: Mongolian traditional bone-setting is a non-surgical treatment method for fractures; however, there is a lack of evidencebased research evaluating its effectiveness, which forms the basis of our study. Aim: To study the therapeutic efficacy of traditional bonesetting treatment for surgical neck fractures of the humerus. Materials and Methods: From June 2023 to June 2025, 120 patients diagnosed with surgical neck fractures of the humerus and treated at the Inner Mongolia International Mongolian Hospital were randomly divided into a traditional bone-setting group (n=64) and a surgical treatment group (n=56). Shoulder and upper limb function were evaluated at 1, 3, and 6 months after treatment using the Constant–Murley score, Oxford Shoulder Score (OSS), and Visual Analog Scale (VAS) for pain. Result: A total of 120 patients with surgical neck fractures of the humerus were divided into two groups, with no significant differences in the baseline characteristics (p>0.05).In the traditional bone-setting group, pain (VAS) decreased more rapidly at 1–3 months (p<0.01), while activities of daily living and functional outcomes improved significantly at 3–6 months (p<0.01). At 6 months, shoulder function (Constant–Murley score) was significantly improved (p=0.049).In contrast, the surgical group showed faster recovery in joint range of motion and muscle strength in the early stage; however, by 6 months, the outcomes between the two groups were comparable. Conclusion In the treatment of surgical neck fractures of the humerus, no significant difference was observed between traditional bone-setting and surgical treatment in terms of shoulder range of motion and muscle strength recovery. However, the advantages of traditional bone-setting lie in its ability to relieve pain more rapidly, promote faster functional recovery, improve activities of daily living, and achieve better long-term outcomes than modern bone-setting. Therefore, traditional bone setting is a safe and effective treatment method that aligns with modern theories of bone healing and rehabilitation principles and holds significant value for wider clinical application and further research in the management of surgical neck fractures of the humerus.

Background: Saliva is a biological fluid that is constantly secreted into the oral cavity and serves to cleanse, moisturize, digest food, maintain a balance of microorganisms, and provide antibacterial, antifungal, and antiviral services during chewing and swallowing. It is also a biological fluid with many components that is suitable for the early detection of various oral diseases. Since there has been little clinical research on the effects of locally produced, organic toothpastes that are widely used in Mongolia on the oral environment, our study was based on saliva analysis and crystallization. Aim: To study the effects of some toothpastes used in Mongolia on the teeth and oral environment Materials and Methods: In accordance with the approval of the Research Ethics Committee of the Mongolian National University of Health Sciences (No. 26/03-B01), we divided 104 children aged 15-17 into 4 groups, collected questionnaires, performed oral examinations, and assessed the risk of DC and PD using SMT and saliva crystallization analysis. The results of the research were processed using STATA and EXCEL programs. Results: The prevalence of dental caries in the study participants was 88.5%, and the intensity was 5.56±4.3 DFM/T. The risk of developing dental caries was moderate, with type 2 dominating (55%). In repeated measurements, the bacterial count in the control group increased from 28.17 to 50.72. The parameters were stable in the eezgii paste group, the white blood cells decreased in the salt paste group. The plaque parameter decreased statistically significantly in the experimental groups (p=0.001). Conclusion Mongolian-produced eezgii paste reduced the number of salivary bacteria (44.88-44.44) and changed the crystallographic form (44% from type 2 to type 1, and 60% from type 3 to type 2). However, salt paste reduced the number of salivary white blood cells (43.16-33.96), while ammonia levels increased by 41.52-48.04 in the clove leaf paste group.

Background: Squamous cell carcinoma, which accounts for 90-95% of oral cancer, arises from the uncontrolled and disordered growth of keratinocyte cells in the mucous of lip and oral cavity. It occupies 2-3% of all cancers and 38% of head and neck cancers. Aim: To study recurrence and prognosis of patients with tongue squamous cell carcinoma Materials and Мethods: Our study, based on retrospective cohort method was conducted from medical reports of 207 patients who were diagnosed with squamous cell carcinoma of oral cancer and at the Department of Head and Neck Surgery, Radio and Chemotherapy of the National Cancer Center of Mongolia between 2009-2019 years. All statistical analysis were carried out using STATA version 15.0 and we performed survival analysis using Kaplan-Meier method. All statistical tests were two sided, and P-values less than 0.05 were considered significant. The study was approved by the Research Ethics Committee of the Mongolian National University of Medical Sciences on June 08, 2021 (№ 2021/3-07). Results: A total of 207 oral cancer cases were registered during the study period. The most common site of oral cancer was the tongue (47.3%, 98/207). Of these, cases 69.4% (68) presented in males and 30.6% (30) in females. At the time of diagnosis, 77 patients (78.5%) were in late stage (III, IV) and 76 cases (77.6%) were well-differentiated. During the study period, 20 patients (20.4%) experienced tongue cancer recurrence; female patients (18.8%, 3/20) had significantly less chance of recurrence compared to men (81.2%, 17/20), (p=0.033). Patients over 61 years old had higher oral cancer recurrence than patients under 60 years, but there was no significant difference in age groups. 90.6% (18/20) of tongue cancer recurrence had with lymph node metastasis; compared to patients without lymph node metastasis (9.4%, 2/20), tongue cancer recurrence was significantly higher (p=0.025). And there was a statistical difference in tongue cancer recurrence percentage among clinical stages (p=0.003). Thirteen (69.4%) patients diagnosed in stage IV had oral cancer recurrence. Pathological grading, and tumor size did not significantly influence cancer recurrence. Conclusion 1. The gender, advanced stage and lymph node metastasis of patients with tongue cancer were significantly associated with the presence of tongue cancer recurrence. (p=0.033, p=0.025, p=0.003). 2. The 5-year survival rate for tongue cancer was the lowest among the oral cancer, which we consider to be due to factors such as late-stage diagnosis, and anatomical characteristics of tongue.

Introduction: According to epidemiological studies conducted in Mongolia over the past decade, diseases of the digestive system have consistently ranked second in terms of morbidity and fourth in mortality. With the rapid advancement of technology, sedentary lifestyles and unhealthy living habits have become more prevalent among the population, leading to a trend of digestive disorders affecting younger age groups. Therefore, investigating the prevalence, pathological changes, and risk factors associated with gallbladder and pancreatic diseases among university students has become the rationale for this study. Objective: To study the prevalence of gallbladder and pancreatic disorders and determine some of the associated risk factors. Methodology: 200 students from the first to sixth year were selected through random sampling based on the WHO age classification. Data collection included a structured questionnaire and ultrasound examination using the SUN-800D device. Diagnostic criteria followed the national clinical guidelines for acute cholecystitis. Results: Out of 200 participants, 120 (60%) were female and 80 (40%) were male. Among them, 128 students (64%) reported skipping breakfast regularly, and 147 (73.5%) did not consume an adequate amount of fluids daily. Ultrasound examinations revealed that 104 students (52%) had some form of gallbladder or pancreatic abnormality. Additionally, gallstones were detected in 1 student (0.5%), and gallbladder polyps were found in 5 students (2.5%). Conclusion Gallbladder and pancreatic abnormalities were observed in 52% of the participants. Among the associated risk factors, 64% of students reported skipping breakfast, and 73.5% had inadequate daily fluid intake. The ultrasound findings showed that 47% had biliary sludge, 32% had gallbladder kinking, and 48.5% had increased pancreatic echogenicity.

Introduction: The development of analytical methods for evaluation of quality standard parameters of drugs and other health prophylactic, diagnostic, and therapeutic products is essential from the beginning in the process to release to the market finished product. Validation of developed analytical methods is one crucial part of the analysis.
Quantitative determination methods of the total main active compounds of the tincture prepared from the root and the herb of Paeonia anomala L. were developed. In order to verify whether the analytical procedure is suitable for its intended purpose parameters – accuracy, reproducibility, intermediate precision, and linearity were validated. Materials and methods: The total phenolics in the tincture were determined by the reaction with Folin Chicolteau reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively. Based on the achieved results of the developed quantitative methods which were validated according to the accepted and pursued technical documents in our country the Eurachem guide “The Fitness for Purpose of Analytical Methods” and Russian Pharmacopoeia 13 “Validation of analytical methodology”, where parameters as accuracy, precision – reproducibility, intermediate precision, and linearity are involved. Conclusions Statistical analysis of total phenolic compounds and total monoterpene glycosides in the Paeonia anomala tincture demonstrated that the bias calculated was for accuracy 0.674% and 0.703%, relative standard deviations were for reproducibility 0.896% and 0.798%, for inter- mediate precision 0.922% and 0.696%, respectively and the linearity – R²=0.9985 and R²=0.9997. All achieved results validated the appropriateness of the developed quantitative methods for the intended purpose.

Background: Traditional Mongolian medicine (TMM), which is the main knowledge of medicine of ancient nomads, has produced hundreds of healers, practitioners, doctors, and scientists in the course of 5,000 years of historical development. The methods developed to diagnose, treat, and prevent diseases are used to protect the health of the population even today. Based on the study of medical records and artifacts, it is possible to determine which diseases were prevalent and use archeological finds and records related to the diseases to determine what treatment methods were used. Research goal: To clarify reports, facts, and evidence on spinal disorders and their treatment methods from ancient books written in Tibetan, Manchu, and Mongolian languages on Traditional Mongolian medicine. Methods: The source study method, hermeneutic method, and a method of comparison were used in studying traditional medical books, research literature, and other related materials. Conclusion Research has shown that the structure, function, pathological changes, diagnosis, and treatment methods of the spine have been recorded in many ancient records written in Tibetan, Manchu, and Mongolian languages on Traditional Mongolian medicine. At the same time, since ancient times, Mongolians have been adapting to the transformation of natural phenomena, and drastic changes in climate and developed methods of treating injuries and diseases caused by the characteristics of life, habits, and war, as well as established an independent system of methods for studying and treating the structure of the human body and living a healthy life by accumulating experience passed down from generation to generation. This system was not only based on the knowledge of Mongolian dhom treatment, and Indian and Tibetan medicine, but it was also a complex of knowledge that incorporated the ancient scriptures of Western medicine.

Background: Throughout history, Mongolians experienced struggles of ups and downs, creating a comprehensive teaching, knowledge, and system on the art and science of living and healthy living and passing it on to the next generation. Since the middle of the last century, the use and study of traditional knowledge have decreased, and with it, the use has decreased, and some knowledge has almost been forgotten. In particular, many branches of traditional medicine, including manual techniques, surgical methods, techniques related to surgery, the use of tools, and the skills of pressing, bone setting, and massage have declined in value, and some methods have almost been forgotten. Therefore, we have chosen the topic due to the need of exploring the possibility of solving this issue and enriching the history of traditional medicine by elucidating the ancient historical development of Mongolian bone-setting therapy, of which reports and historical documents in Mongolian medicine are rare. Research goal: To clarify the ancient history of Mongolian bone-setting therapy, establishing its features, usage, and types of methods based on historical evidence.
The following methods were used in the research
Herein: the method of researching original documents and reports, the method of comparison, and the method of probabilistic listing. Conclusion
1. Evidence related to ancient Mongolian bone-setting therapy dates back to the Iron Age and can be traced back to 2000 years ago from the traces of illness and injuries, bone ossification, and signs of healing of the ancient nomads living in Chandman mountain of present day Uvs province, the tribe known as Chandman people.
2. The research shows that it is possible to determine which diseases were prevalent based on the study of related artifacts and scriptures related to Traditional Mongolian medicine, and which methods of treatment were used using archaeological finds and scriptures related to the diseases.

Introduction: Due to social transition and change of many societal activities in Mongolia, there is an increasing need for pharmaceutical drugs to treat anxiety, stress, fatigue and insomnia. It is considered that medicinal plants used in traditional medicine as sedatives, anti-anxiety and anti-depressive activities could be effective. Our study focuses on exploring optimal methods for preparing tinctures from Paeonia L. genus species, a sedative in Mongolian traditional medicine, and studying their phytochemical compositions. Materials and methods: Root and herb of Paeonia anomala L., grown in Mogod soum in Bulgan province, were used for the study.
Prepared herb and root were chopped and sieved into 1 mm, 2 mm, 3 mm, 4 mm and 5 mm sizes. Then from each size of two samples, a set was created with a 1:1 ratio. Finally 40 % ethanol (1:10 ratio with a set) was applied to prepare tincture through a traditional maceration method. The obtained tincture was analyzed for its color, quantity of biologically active compounds, dry residue, and absorption coefficient using an optimal particle size of samples, volume of the extractive liquid and tincture yield.
Tincture from the set of herb and root of Paeonia anomala L. was prepared using 3 methods, namely, by the traditional maceration; intensified maceration using the ultrasonic bath processing with 45 kHz at an ambient temperature; constant shaking in a heat of 30o-40oC. Conclusion The more optimal and effective method for preparing tincture from Paeonia anomala L. has been found to be the ultrasonic bath processing with 45 kHz for 6 h at an ambient temperature. Crude drug mixture of 2 mm sizes with 40% ethanol (where volume absorption coefficient was accounted to be 2.8) was mixed at a ratio of 1:10. In the obtained tincture, total phenols were determined as 0.58±0.003% in gallic acid equivalent and total monoterpene glycosides as 1.96±0.002% in paeoniflorin, which were the highest compared to other methods, indicating the method is the more optimal one.

Introduction: It is important to standardize a tincture prepared from the herb and root of Paeonia anomala L, which is widely used as a sedative in traditional medicine, based on the results of the studies its chemical composition, biological and pharmacological activities. Moreover, there is a need to carry out a quantitative stability testing in order to establish the ability to maintain quality under certain storage condition, shelf-life and to develop suitable packaging information. Materials and methods: Standardization parameters of prepared Paeonia anomala tincture have been evaluated according to WHO guidelines for the determination of crude drug material, plant preparations and finished products technical parameters, along with the Mongolian National Pharma- copoeian (MNP) and Russian XIV Pharmacopoeian methods for tincture. The color of a tincture, dry residue, special density, alcohol content, and microbiological content were determined according to the methods described in MNP. A qualitative analysis of biological active constituents has been evaluated by thin-layer chromatography, the total phenolic compounds were determined by the reaction with Folin Chicalteu reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively.
The stability testing study was performed according to the “General requirements for the stability testing study of drug-active compounds” MNS-6439-2014 using the real-time methods for the standardization parameters of the tincture. Conclusions The standardized parameters of tincture prepared from the herb and root of Paeonia anomala L. were approved by the National Reference Laboratory for Food Safety analysis. Consequently, the Mongolian pharmacopoeian article on Paeonia anomala tincture was officially permitted under the number ҮФӨ-0327-2017.
The stability study of tincture was carried out according to the MNS-6439-2014 by real-time tests for 24 months which provided that the changes in tincture quality were within the permitted limits. Consequently, it can conclude that the storage condition and shelf-time of Paeonia anomala tincture are 2 years under not above 25°С.

Introduction: Over 800,000 people in the world contract HCC each year and approximately 700,000 die from the disease. HCC is the 6th most common cancer in the world. HCC is the 3rd leading cause of cancer deaths in the world. 2/3 of liver cancer deaths are caused by hepatitis. In the U.S, HCV infection is the more common cause of HCC, while in Asia and Africa, HBV is more common. Mongolia ranks first in the world in mortality from liver cancer, indicating the need for early detection and treatment of cirrhosis. Sysmex Corporation has introduced for HISCL series analyser, a new cirrhosis marker M2BPGi of non-invasive, blood-testing. In 2016, the test was introduced at Medipas Hospital in Orkhon province. It is possible to study the advantages and significance of the marker for use in clinical practice. Materials and methods: From a total of 385 patients who underwent M2BPGi marker testing in 2016-2017Medipas hospital laboratory, data from a total of 283 patients tested for hepatitis B and C virus and M2BRGi markers were selected. A comparison of age, sex, and test parameters of a total of HCVab and HBsAg positive 172 patients tested for Total bilirubin, GPT, GOT, GGT, AFP and M2BPGi. HCV Ab, HBsAg, AFP, M2BPGi markers were analyzed by SysmexHISCL-5000 fully automated immunological analyzer, Liver function tests were performed with a fully automatic biochemical analyzer JEOL Biomajesty BM6010/C. Results: Of the M2BPGi marker tested 283 patients 94 (33%) were infected with the C virus, 78 (28%) were with the B virus,11 (4%) were co-infected with B and C viruses, 100 (35%) no any viral infection. Of the 172 patients diagnosed with hepatitis B and C virus infection, 97 (56%) were male, 75 (44%) were female. In terms of age, 72% of the population is over 45 years old.
Of the 172 patients, 115 (67%) had M2BPGi marker abnormal or > 1.0 COI. Of the M2BPGi marker abnormal patients, 47 (41%) were infected with the B virus and 68 (59%) with the C virus. In terms of age, 27.7% of hepatitis B patients and 10.3% of hepatitis C patients were under 45 years of age, 72.3% of hepatitis B patients and 89.7% of hepatitis C virus patients were over 45 years of age.
Hepatitis B and C viruses are slightly more common in men than in women. The majority of patients infected with the hepatitis virus over the age of 45. The majority of patients with hepatitis virus have abnormal liver function. Increased M2BPGi markers in people under the age of 45 with hepatitis B virus infection are relatively higher for hepatitis B virus infection than for C virus infection. Conclusions The M2BPGi marker was abnormal in 67% of hepatitis virus infected patients. It has been observed that the probability of an increase in M2BPGi marker is slightly higher in hepatitis C virus infection than in hepatitis B virus infection.