Understanding the patterns and occurrence of influenza is crucial for effective public health responses. National sentinel surveillance systems provide invaluable data that can be used to track the prevalence, seasonality and evolution of influenza viruses, and to inform timely interventions and early warning systems for disease control. This study aimed to describe the trends in influenza-like illness (ILI) and influenza incidence based on data obtained from ILI surveillance conducted among patients presenting to health facilities with symptoms indicative of influenza in Cambodia during 2006–2023. A total of 27 098 samples were tested, of which 15.5% were positive for influenza. Of these, 63.6% were influenza A, 36.2% were influenza B, and 0.2% were both influenza A and B. The annual incidence of influenza among the outpatient population varied, averaging 8.2 cases (range: 0.05–23.8) per 1000 population. Incidence rates were highest in 2007, 2008 and early 2023. The overall positivity rate was 16%. Not all ILI surveillance sites were consistently active during the study period, primarily due to funding constraints. Given the constraints of sentinel site coverage and data collection inconsistency, the study emphasizes the vital need for ongoing and improved ILI surveillance to accurately assess influenza incidence. To gain a more detailed picture of influenza dynamics in Cambodia, surveillance systems should be strengthened and data collection should be expanded to include asymptomatic patients and other important patient characteristics. These results are needed to guide regional and national influenza preparedness, prevention and control initiatives.