Objective To analyze thestatus quo of comprehensive management services for community prevention and treatment of patients with severe mental impairment (SMI). Methods The clinical data of 630 patients with SMI in the hospital from January 2021 to December 2023 were retrospectively analyzed. The types of disease, demographic characteristics, assistance to registered patients, management and treatment of patients were analyzed by descriptive statistics. Chi-square test and chi-square segmentation were used for statistical inference. Results Among the 630 SMI patients, the ratio of male to female was 1:1.23, and patients were mainly distributed in 18-65 years old, more patients were with education level of primary school and junior high school, they were mainly farmers (21.90%) and laid-off or unemployed people (32.38%). Patients without family history accounted for 72.38% (456/630) and non-poor patients accounted for70.48% (444/630).The main types of SMI were schizophrenia (65.71%) and bipolar disorder (18.10%).The guardianship subsidy subjects accounted for89.52%,nearly 40.95% of the patients received the mental disability certificate, 82.86% of the patients were served by the care and assistance group, and 49.52% of the patients participated in community rehabilitation. The management rates of SMI patients in 2021, 2022, and 2023 were 97.14%, 99.05%, and 98.10% respectively. The standardized management rates were 96.19%, 96.19% and 97.14%, and the medication rates were 86.67%, 88.57% and 85.71%, and the regular medication rates were 79.05%, 81.90% and 80.95% respectively. Conclusion The prevention and treatment of schizophrenia-based serious mental illness has become an important task in this district. The overall management status of SMI is good, with a stable rate of management and standardized management of patients, but there is still a need to strengthen the management of the medication rate and the regularity of the medication rate of patients with serious mental illness, and at the same time, there is a need to intensify the publicity efforts on mental health and to increase the awareness of the prevention of the disease.

With the popularization of small incision lenticule extraction(SMILE), a large number of one-piece corneal stromal lenticules are removed during surgery. As an additional product of surgery, the experimental research and clinical reuse of extracted corneal stromal lenticules from SMILE have become a research hotspot in recent years. Corneal stromal lenticules are thin and transparent, rich in sources, low in cost, and have the advantages of low immunogenicity and good tissue compatibility, so they can be used as important source of cells and corneal stroma research, and also can be used as good biomaterials for corneal reinforcement, patch graft, refractive correction, and lacrimal duct embolization in clinical study. This article reviews the clinical application of SMILE-derived lenticule in the treatment of corneal-related diseases, correction of hyperopia and presbyopia, coverage of glaucoma drainage valve to prevent their exposure, and its use in lacrimal duct plug, aiming to fully recognize the reuse of lenticules in clinical practice, expand its surgical indication, and providing new directions for the treatment of other eye diseases and its application in tissues and organs.

Objective:To evaluate and summarize the best evidence on fatigue management in children with tumors both domestically and internationally, providing reference for medical and nursing staff to improve fatigue symptoms in children.Methods:The evidence on fatigue management in children with tumors, including best practices, recommended practices, guidelines, systematic reviews, evidence summaries, and expert consensus, was systematically retrieved from clinical decision support systems, guideline websites, professional association websites, and databases both domestically and internationally. The search period was from database establishment to April 2023. Two researchers independently conducted literature quality evaluation and evidence extraction.Results:A total of 17 articles were included, including four guidelines and 13 systematic reviews. Thirty-two best pieces of evidence were extracted from six aspects of assessment and screening, identification of risk factors, health education, exercise intervention, medication intervention, and other interventions of fatigue in children with tumors.Conclusions:The best evidence for fatigue management in children with tumors is summarized, which can provide a basis for medical and nursing staff to improve their fatigue symptoms. It is recommended that medical and nursing staff combine clinical context, professional opinions, and patient wishes to screen the best evidence and develop personalized fatigue management programs.

Objective: To prepare monoclonal antibodies against pneumonia serotype 33F polysaccharides (Pn33Fps) and hepatitis B virus (HBV) surface proteins (HBs) by using the conjugate of Pn33Fps and HBs as antigen. Methods: The conjugate of Pn33Fps and HBs was used as antigen to immunize mice with different immune doses, different immune procedures and different immune sites. Mouse spleen cells with higher antibody level after immunization were isolated and fused with SP2/0 myeloma cells. The hybridoma cells were screened specifically with Pn33Fps or HBs to prepare corresponding monoclonal antibodies. Results: Serum antibodies against Pn33Fps and HBs were induced by immunizing mice with the conjugate. Monoclonal cell lines capable of continuously expressing antibodies against Pn33Fps or HBs were obtained. It has been proved that the recovery rates of samples of Pn33Fps and HBs prepared in three batches tested with ascites monoclonal antibodies prepared by these two monoclonal cell lines were between 95% and 105%. Conclusions Monoclonal antibodies against Pn33Fps and HBs could be prepared simultaneously by immunizing mice with the conjugate of Pn33Fps and HBs and used for the quantitative detection of Pn33Fps and HBs.

Objective? To search and summarize the best evidence of nursing management of procedural pain in pediatric patients so as to provide guidance for clinical nursing staff in the standardized prevention and management of procedural pain. Methods? The Cochrane Database of Systematic Reviews, JBI Database of Systematic Reviews, National Guideline Clearinghouse(NGC), the National Institute for Health and Care Excellence(NICE) and Scottish Intercollegiate Guideline Network(SIGN) were searched on computer to collect literature including clinical practice guidelines, evidence summary and systemic reviews about the management of procedural pain among children 3 years old above. Two researchers independently assessed the quality and extracted data and conducted evidence extraction from the articles that met the standard of quality. Results? Totally eleven articles were enrolled including three best practice guidelines, three systematic reviews and five evidence summaries. Conclusions? Cognitive behavioral intervention, topical application of epidermal anesthetics or local cold compress, and combined operation of distraction can effectively reduce the pain and anxiety caused by procedures. The knowledge and skills of procedural pain management should be included in the education and training courses of medical institutions. Evidence should be selected pertinently to participate in the management of procedural pain in the mode of multidisciplinary cooperation.

Objective To study the role of recombinant human epidermal growth factor (rhEGF) in the prognosis of multiple organ dysfunction syndrome (MODS) in mice. Methods One hundred and twenty clean male Kunming mice were randomly ( random number) divided into normal saline control group (n =15),MODS model control group (n =15) and MODS + rhEGF treatment group (n =90).The MODS models were made by using Caballero ME method with thioacetamide (TAA) 2000 mg/kg injected intraperitoneally to establish monophasic rapid onset pattern of MODS model in mice.MODS + rhEGF treatment group was further randomly divided into two subgroups,namely intraperitoneal injection group (n =45 ) and subcutaneous injection group (n =45 ).Each subgroup was divided again into three small subgroups (n =15) as per different doses of rhEGF used,namely 10 μg/kg,30 μg/kg and 50 μg/kg.Within 24 hours after modeling,the respiration,body weight,food eaten and general physical changes were observed.Mortality was calculated 24 hours after modeling.After the animals sacrificed,the tissues of viscus including liver,kidney,heart,brain,lung,spleen,pancreas,intestine and stomach were collected immediately.The histological changes of visceral tissues were studied by using hematoxylin -eosin staining under the light microscope.All the experimental data were presented in,and body weight changes were compared using t-test,and after different routes of administration with different doses of rhEGF used in MODS,the mice body weight changes were analysed by using the Dunnett method,and the mortalities of mice were compared by using Fisher exact test,and P ＜ 0.05 was considered statistically significant difference. Results There was no significant difference in mortality betweeu mice in rhEGF subcutaneous administration group and MODS model control group (P ＞ 0.05 ),but the total mortality of hrEGF MODS intraperitoneal administration group (6.7％ in dose of 50 μg/kg and 20％ in dose of 30 μg/kg) was significantly lower than that of MODS model control group (73.3％) ( P ＜ 0.05 ) and the mortality of mice treated with intraperitoneal 50μg/kg rhEGF (6.7％ ) was lower than that treated with 10μg/kg rhEGF (P=0.014).The mortality of mice in rhEGF MODS (50 μg/kg ) intraperitoneal administration group was significantly lower than that in subcutaneous administration group (40％) (P =0.031 ), The histopathological changes in rhEGF MODS treatment group were not as remarkable as seen in mice of control group.The histopathological changes were dose - dependent.The higher doses of rhEGF,the lesser hepatic congestion,liver cell apoptosis,hepatic cell cloudy swelling and cell vacuolization.Similarly,as RhEGF dosage increased,pulmonary interstitial congestion,inflammatory cells and apoptotic bodies reduced,and bronchial ciliated columnar epithelium less shed.Conclusions RhEGF plays a positive role in repairement of tissue damage in TAA - induced MODS murine model.The rhEGF given by intraperitoneal route of administration is more effective to reduce the 24 h mortality of MODS mice than that by subcutaneous route.

Objective To investigate the preparation and stability of compound cefaclor tablets.Methods The compound cefaclor tablets were prepared.The quality standard Were established.A HPLC method WaS established to determine the content and stability of samples.Results The prepared tablets was stable and the quality could be controlled.Conclusion The tablets were of reasonable formulation,good sample technology and good stability.The established method is accurate and reliable for the dissolution test for compound cefaclor tablets.The method can be used for quality control of compound cefaclor tablets.