[Objective]This study aims to investigate the effects of methionine sulfoxide reductase A(MSRA)on the proliferation,apoptosis,invasion,and metastasis of clear cell renal cell carcinoma(ccRCC)through cellular and animal experiments to elucidate its potential biological mechanisms,providing new molecular targets and strategies for the treatment of ccRCC.[Methods]MSRA-overexpressing cell lines were constructed with transfection.The effects of MSRA on the proliferation,apoptosis,invasion,and migration of ccRCC cells were assessed through proliferation assays,colony formation assays,apoptosis assays,wound healing assays,and invasion assays.Further,the mechanisms and related signaling pathways were explored via ROS detection,RT-qPCR,and Western blot.Subsequently,a subcutaneous xenograft mouse model was employed to verify the effect of MSRA on ccRCC tumor growth and metastasis at the animal level to explore its potential molecular mechanisms.[Results]The mRNA and protein expressions of MSRA in OS-RC-2 and 786-O cell lines were low(RT-qPCR:0.57±0.09,0.56±0.04;WB:0.26±0.13,0.24±0.09).RT-qPCR and Western blot experiments confirmed the successful construction of OS-RC-2-pLVSO2-MSRA(RT-qPCR:108.04±1.80;WB:117.01±20.19)and 786-O-pLVSO2-MSRA(973.45±51.37;WB:190.34±30.13)overexpressed cell lines,with statistically significant differences(P＜0.001).Proliferation assays showed reduced proliferation in OS-RC-2-pLVSO2-MSRA(72 h:1.246±0.003)and 786-O-pLVSO2-MSRA(72 h:1.468±0.001),with significant differences(P＜0.001).Colony formation assays revealed a decrease in colony numbers in OS-RC-2-pLVSO2-MSRA(0.090±0.002)and 786-O-pLVSO2-MSRA(0.080±0.002),with significant differences(P＜0.001).Apoptosis assays demonstrated increased apoptosis rates in OS-RC-2-pLVSO2-MSRA(2.013±0.116)and 786-O-pLVSO2-MSRA(4.767±0.199),with significant differences(P＜0.001).Wound healing assays indicated less migration distance in OS-RC-2-pLVSO2-MSRA(0.643±0.028)and 786-O-pLVSO2-MSRA(0.603±0.034),with significant differences(P＜0.001).Transwell assays showed a reduction in the numbers of penetrative cells in OS-RC-2-pLVSO2-MSRA(16.80±2.28)and 786-O-pLVSO2-MSRA(21.40±4.78),with significant differences(P＜0.001).Fluorescence assays indicated a decreased ROS levels in OS-RC-2-pLVSO2-MSRA(50.59±6.24)and 786-O-pLVSO2-MSRA(62.87±5.35),with significant differences(P＜0.001).Western blot analysis showed a decrease in the expression of N-cadherin and Vimentin,and an increase in the expression of E-cadherin in OS-RC-2-pLVSO2-MSRA and 786-O-pLVSO2-MSRA(P＜0.001).Western blot analysis revealed a significant decrease in the expression levels of p-ERK1/2 and p-SMAD3 in OS-RC-2-pLVSO2-MSRA and 786-O-pLVSO2-MSRA(P＜0.001).Animal experiments showed reduced tumor volumes in OS-RC-2-pLVSO2-MSRA(155.00±50.46),with significant differences(P＜0.001).Western blot analysis of tumor tissues from animals confirmed the decreased expression of N-cadherin and Vimentin,and the increased expression of E-cadherin,with significant differences(P＜0.001).IHC experiments of OS-RC-2-pLVSO2-MSRA tumors revealed a decrease in the expression of Ki-67,N-cadherin,and Vimentin,and an increase in the expression of E-cadherin,with significant differences(P＜0.001).[Conclusion]MSRA overexpression inhibits ROS expression in ccRCC,suppresses the EMT process,and consequently inhibits the proliferation,invasion,and metastasis of ccRCC.

[Objective]This study aims to investigate the effects of methionine sulfoxide reductase A(MSRA)on the proliferation,apoptosis,invasion,and metastasis of clear cell renal cell carcinoma(ccRCC)through cellular and animal experiments to elucidate its potential biological mechanisms,providing new molecular targets and strategies for the treatment of ccRCC.[Methods]MSRA-overexpressing cell lines were constructed with transfection.The effects of MSRA on the proliferation,apoptosis,invasion,and migration of ccRCC cells were assessed through proliferation assays,colony formation assays,apoptosis assays,wound healing assays,and invasion assays.Further,the mechanisms and related signaling pathways were explored via ROS detection,RT-qPCR,and Western blot.Subsequently,a subcutaneous xenograft mouse model was employed to verify the effect of MSRA on ccRCC tumor growth and metastasis at the animal level to explore its potential molecular mechanisms.[Results]The mRNA and protein expressions of MSRA in OS-RC-2 and 786-O cell lines were low(RT-qPCR:0.57±0.09,0.56±0.04;WB:0.26±0.13,0.24±0.09).RT-qPCR and Western blot experiments confirmed the successful construction of OS-RC-2-pLVSO2-MSRA(RT-qPCR:108.04±1.80;WB:117.01±20.19)and 786-O-pLVSO2-MSRA(973.45±51.37;WB:190.34±30.13)overexpressed cell lines,with statistically significant differences(P＜0.001).Proliferation assays showed reduced proliferation in OS-RC-2-pLVSO2-MSRA(72 h:1.246±0.003)and 786-O-pLVSO2-MSRA(72 h:1.468±0.001),with significant differences(P＜0.001).Colony formation assays revealed a decrease in colony numbers in OS-RC-2-pLVSO2-MSRA(0.090±0.002)and 786-O-pLVSO2-MSRA(0.080±0.002),with significant differences(P＜0.001).Apoptosis assays demonstrated increased apoptosis rates in OS-RC-2-pLVSO2-MSRA(2.013±0.116)and 786-O-pLVSO2-MSRA(4.767±0.199),with significant differences(P＜0.001).Wound healing assays indicated less migration distance in OS-RC-2-pLVSO2-MSRA(0.643±0.028)and 786-O-pLVSO2-MSRA(0.603±0.034),with significant differences(P＜0.001).Transwell assays showed a reduction in the numbers of penetrative cells in OS-RC-2-pLVSO2-MSRA(16.80±2.28)and 786-O-pLVSO2-MSRA(21.40±4.78),with significant differences(P＜0.001).Fluorescence assays indicated a decreased ROS levels in OS-RC-2-pLVSO2-MSRA(50.59±6.24)and 786-O-pLVSO2-MSRA(62.87±5.35),with significant differences(P＜0.001).Western blot analysis showed a decrease in the expression of N-cadherin and Vimentin,and an increase in the expression of E-cadherin in OS-RC-2-pLVSO2-MSRA and 786-O-pLVSO2-MSRA(P＜0.001).Western blot analysis revealed a significant decrease in the expression levels of p-ERK1/2 and p-SMAD3 in OS-RC-2-pLVSO2-MSRA and 786-O-pLVSO2-MSRA(P＜0.001).Animal experiments showed reduced tumor volumes in OS-RC-2-pLVSO2-MSRA(155.00±50.46),with significant differences(P＜0.001).Western blot analysis of tumor tissues from animals confirmed the decreased expression of N-cadherin and Vimentin,and the increased expression of E-cadherin,with significant differences(P＜0.001).IHC experiments of OS-RC-2-pLVSO2-MSRA tumors revealed a decrease in the expression of Ki-67,N-cadherin,and Vimentin,and an increase in the expression of E-cadherin,with significant differences(P＜0.001).[Conclusion]MSRA overexpression inhibits ROS expression in ccRCC,suppresses the EMT process,and consequently inhibits the proliferation,invasion,and metastasis of ccRCC.

Regional odontodysplasia (ROD) is a rare localized dental developmental anomaly. The typical clinical manifestations of ROD are abnormal tooth eruption, abnormal development of enamel and dentin. The radiographic characteristic is "ghost teeth". Its etiology still remains unknown. The care and treatment of a patient with ROD needs a multidisciplinary approach. And the treatment should be taken after the assessment of each individual case of ROD. This paper reviews the definition, etiology, epidemiological features, clinical manifestations, imaging features, dental microstructure and treatment strategies of ROD to provide reference for clinical diagnosis and treatment.

Objective:To determine the effects of pentoxifylline-tocopherol-clodronate combination (PENTOCLO) protocol in the treatment of localized temporal bone osteoradionecrosis (TBORN).Methods:A retrospective analysis was conducted on the clinical data of 21 patients, who suffered localized TBORN (23 ears) and were treated with the PENTOCLO protocol in Sun Yat-sen Memorial Hospital, Sun Yat-sen University from November 2020 to April 2021. The curative effects of the PENTOCLO protocol were evaluated based on the changes in ear symptoms and the extent of exposed bone before and after treatment.Results:The PENTOCLO protocol was applied for (506 ± 48) d on average. As a result, 19 ears (82.6%) became free of earache and purulent ear discharge, and two ears (8.7%) showed alleviation of symptoms. Moreover, nine ears (39.1%) exhibited re-epithelialization in the ear canal, 11 ears (47.6%) showed a decrease in exposed ear canal bone, and the osteonecrosis of three ears (13.0%) was stable.Conclusions:PENTOCLO has encouraging treatment effects on TBORN, and thus can be used as an effective nonsurgical option for localized TBORN.

Objective:To investigate the clinical effect of the bell-bottom flap in the treatment of multiple little toes with syndactyly deformity of the 4th and 5th toes.Methods:Forty cases (22 males and 18 females) of multiple little toes with syndactyly deformity of the 4th and 5th toes were treated in the Plastic Surgery Department of Fujian Maternal and Child Health Hospital from June 2014 to May 2020. The age of patients ranged from 6 months to 36 months, with an average of 11 months. There were 46 feet, among which 19 cases had a complete fusion, and 27 cases had an incomplete fusion. During the operation, the polydactyly was removed, the fibula-deviation deformity of little toes was corrected with the articular surface formation and joint capsule repaired. The bell-bottom flap was used to reconstruct the webbed of the toe, and the wound surface on the side was closed with three flaps made up of the residual skin soft tissue of multiple little toes. Meanwhile, the soft tissue length at the distal end of the little toe was extended, and the toenail fold and palmar flexion transverse lines were reconstructed to form a similar appearance to the normal.Results:All the flaps in all 46 feet survived with no flap necrosis, skin grafting, or infection. After 6 to 36 months of follow-up (averagely, 20 months), the width, depth, and slope of the reconstructed toe webbed were normal or close to normal, the fibula deviation of the small toe was corrected entirely, the deformity of the short toe was improved in varying degrees, no toe scar contracture, and the appearance of nail fold was consistent with that of the normal small toe.Conclusions:Bell-bottom flap is a good method to correct the deformities of small toe polysyndactyly, which can reconstruct the well-formed webbed, no skin grafting, with the shape of small toenail fold and small toe naturally, and the radians of palmar flexion line naturally.

Objective:To investigate the psychological stress and related factors of community medical staff in Shanghai during infectious public health emergencies.Methods:Random cluster sampling, questionnaires combining general demographic information, self-rating anxiety scale(SAS), self-rating depression scale(SDS)and yale-brown obsessive compulsive scale(YB)were administrated to investigate the psychological stress of medical staff from 20 community healthcare centers in 10 Shanghai districts.Results:A total of 696 valid questionnaires were collected. 17.36% of the staff had SAS scores exceeding the boundary value. Nurses, staff with per capita monthly family income<5 000 yuan, or with below-average family relations presented higher SAS scores. 28.19% of the staff had SDS scores exceeding the boundary value. Staff with 5-10 years seniority, nurses, with per capita monthly family income<5 000 yuan, and with poor family relations presented higher SDS scores. 6.68% of the staff had YB score≥16. Nurses presented a higher YB score.Conclusions:Some community healthcare center staff experienced psychological stress during public health emergencies.Factors associated with psychological stress, such as 5-10 years′ seniority, nurses, per capita monthly family income<5 000 yuan, below-average or poor family relations, deserve more attention in order to prevent from or alleviate harmful psychological stress.

Objective:To investigate the clinical effect of the bell-bottom flap in the treatment of multiple little toes with syndactyly deformity of the 4th and 5th toes.Methods:Forty cases (22 males and 18 females) of multiple little toes with syndactyly deformity of the 4th and 5th toes were treated in the Plastic Surgery Department of Fujian Maternal and Child Health Hospital from June 2014 to May 2020. The age of patients ranged from 6 months to 36 months, with an average of 11 months. There were 46 feet, among which 19 cases had a complete fusion, and 27 cases had an incomplete fusion. During the operation, the polydactyly was removed, the fibula-deviation deformity of little toes was corrected with the articular surface formation and joint capsule repaired. The bell-bottom flap was used to reconstruct the webbed of the toe, and the wound surface on the side was closed with three flaps made up of the residual skin soft tissue of multiple little toes. Meanwhile, the soft tissue length at the distal end of the little toe was extended, and the toenail fold and palmar flexion transverse lines were reconstructed to form a similar appearance to the normal.Results:All the flaps in all 46 feet survived with no flap necrosis, skin grafting, or infection. After 6 to 36 months of follow-up (averagely, 20 months), the width, depth, and slope of the reconstructed toe webbed were normal or close to normal, the fibula deviation of the small toe was corrected entirely, the deformity of the short toe was improved in varying degrees, no toe scar contracture, and the appearance of nail fold was consistent with that of the normal small toe.Conclusions:Bell-bottom flap is a good method to correct the deformities of small toe polysyndactyly, which can reconstruct the well-formed webbed, no skin grafting, with the shape of small toenail fold and small toe naturally, and the radians of palmar flexion line naturally.

Objective To establish a long-term survival model of deep hypothermic circulatory arrest (DHCA) in rats,which could contribute to the research of organ damage mechanism and clinical treatments related to DHCA.Methods Twenty Sprague-Dawley rats were randomly (random number) divided into the sham group (n=10) and DHCA group (n=10).After anesthesia,a 20 G catheter was cannulated in the tail artery for arterial inflow,a multiorificed catheter in the right jugular vein for venous drainage,and a 24G catheter in the branch of left femoral artery for artery blood pressure monitoring.Rats in the DHCA group underwent DHCA procedure for 40 min after brain temperature cooled to 18℃,then rewarmed for 40 min,till the brain temperature were above 34℃.Rats in the sham group were cannulated but did not undergo cardiopulmonary bypass (CPB).Hemodynamic parameters and blood gas analysis were measured for 5 times (pre-CPB,15 min after CPB,10 min after rewarming,40 min after rewarming,and 30 min after CPB).Results One rat in the DHCA group died,and the rest rats survived.The lactate level in the DHCA group after rewarming during operation was significantly higher than that in the sham group (7.84 mmol/L vs 1.93 mmol/L,P＜0.05).Conclusions In this study,40-min DHCA model in rats is characterized by safe and long-term survival.

Objective: The purpose of this study was to explore the relationship between maternal tea consumption and birth outcomes. Methods: From January 2005 to December 2010, pregnant women were recruited from Nanjing Maternity and Child Health Hospital. The basic information and the situation of tea consumption during pregnancy were investigated using questionnaire and the birth outcomes of newborns were followed up. Finally, 500 pairs of mothers and infants with complete and standard-compliant data were included in the analysis. The differences of birth outcomes between the tea consumption group and the non tea consumption group were compared and the associations between tea consumption and birth outcomes were analyzed using multivariate logistic regression. Results: Mother's tea consumption rate was 32.8% (164 cases) during pregnancy. The rate of low birth weight in the tea consumption group was (5.5%, 9 cases) and higher than that in the non-tea consumption group (2.1%, 7 cases) (P=0.042). There was no significant difference in the rate of premature birth, small for gestational age, fetal distress, and macrosomia between the two groups After the adjustment of maternal age, education level, family income, weight gain during pregnancy, pre-pregnancy BMI, parity, gestational hypertension, gestational diabetes, neonatal gender and gestational age, compared with non-tea consumption group, there was a positive effect on low birth weight, OR(95%CI) was 4.76 (1.06-21.48). The OR (95%CI) value of the low birth weight risk of the tea group was 5.30 (1.04-26.92) compared with the non-tea consumption group after the adjustment of additional factors such as passive smoking, coffee consumption, folic acid supplement, mineral supplement, carbonated beverage consumption. Simultaneously, compared with non-tea consumption group, there was no statistically significant association between tea consumption during pregnancy and premature birth, small for gestational age, fetal distress and macrosomia (P>0.05). Conclusion Tea consumption during pregnancy was a risk factor for low birth weight in offspring.

Objective To analyze the antimicrobial resistance profile of clinical isolates in Shanghai Xinhua Hospital Chongming Branch affiliated to Shanghai Jiaotong University School of Medicine , a member of China Antimicrobial Resistance Surveillance System, during 2015, for the purpose to facilitate rational antimicrobial therapy. Methods Strain identification?and?susceptibility?testing?were?carried?out?for?the?clinical?isolates?using?MicroScan?WalkAway?96?Automated?Systems and Kirby-Bauer method. Results In 2015, a total of 1815 isolates were collected, including gram-negative bacteria (73.2 %) and gram-positive bacteria (26.8 %). The top three frequently isolated species were Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. ESBL-producing strains were found in 36.3 % of the Escherichia coli isolates, 12.6 % of the Klebsiella (K. pneumoniae and K. oxytoca) isolates, and 28.0 % of the Proteus mirabilis isolates. The prevalence of carbapenem-resistant strains was 0.69 % in Enterobacteriaceae isolates. The prevalence of methicillin-resistant strain was 29.1 % in S. aureus, and 61.4 % in coagulase-negative Staphylococcus isolates. No more than 15 % of the Enterobacteriaceae isolates and no more than 20 % of the P. aeruginosa and Acinetobacter isolates were resistant to carbapenems. No vancomycin-or linezolid-resistant strains were found in Enterococcus or Staphylococcus. Conclusions Antibiotic-resistant clinical isolates are a serious threat for clinical antimicrobial treatment. We should pay more attention to such urgent situation and rational use of antibiotics.