Objective Network pharmacology,Molecular docking and Label-free DIA quantitative phosphoproteomics were used to reveal the potential mechanism of Peiminine against colon cancer.Methods 1The target of peiminine was obtained by SwissTargetPrediction,TargetNet and pharmmapper,and the target of colon cancer was obtained by DisGeNET,GeneCards and OMIM.Then the intersection target of Peiminine and Colon cancer was obtained by Venny2.1.0 online platform.Then,String database and Cytoscape3.8.2 software were used to map the PPI network of intersection targets,and the main targets of Peiminine against Colon cancer were obtained.GO analysis and KEGG pathway analysis were carried out through David database and Weisenxin visual cloud platform.② MOE(molecular operating environment)software was used to perform molecular docking of peiminine and the main target.③ Label-free DIA quantitative phosphoproteomics was used to detect and analyze the biological function of DT group(DT1-DT3)treated with Peiminine and control group(NC1-NC3).Results ① There were 275 intersection targets between peiminine and colon cancer.Molecular docking showed that peiminine could stably dock and interact with the protein structures of AKT1,EGFR,HSP90AA1 and SRC:Peiminine interacted with the amino acid residues of AKT1 mainly through hydrogen bonding.Peiminine interacted with amino acid residues of EGFR,HSP90AA1 and SRC mainly through ionic bond and hydrogen bonding.② Phosphoproteomics analysis showed that:Compared with the NC group,880 phosphorylated modification sites were significantly up-regulated in the DT group(including S124 and S126 sites of AKT1 and T648 and S643 sites of EGFR),and 425 phosphorylated modification sites were significantly down-regulated in the DT group(including T317 sites of HSP90AA1).③ Comparing the results of network pharmacology and phosphoproteomics analysis,it was found that:The main targets of Peiminine against Colon cancer are AKT1,EGFR and HSP90AA1.It promotes apoptosis of colon cancer cells by regulating 17 pathways including AMPK signaling pathway,mTOR signaling pathway and Choline metabolism in cancer.Conclusion This study revealed the potential mechanism of peiminine in the treatment of colon cancer with multiple targets and multiple pathways,and provided a certain direction and reference for subsequent research.

Objective Network pharmacology,Molecular docking and Label-free DIA quantitative phosphoproteomics were used to reveal the potential mechanism of Peiminine against colon cancer.Methods 1The target of peiminine was obtained by SwissTargetPrediction,TargetNet and pharmmapper,and the target of colon cancer was obtained by DisGeNET,GeneCards and OMIM.Then the intersection target of Peiminine and Colon cancer was obtained by Venny2.1.0 online platform.Then,String database and Cytoscape3.8.2 software were used to map the PPI network of intersection targets,and the main targets of Peiminine against Colon cancer were obtained.GO analysis and KEGG pathway analysis were carried out through David database and Weisenxin visual cloud platform.② MOE(molecular operating environment)software was used to perform molecular docking of peiminine and the main target.③ Label-free DIA quantitative phosphoproteomics was used to detect and analyze the biological function of DT group(DT1-DT3)treated with Peiminine and control group(NC1-NC3).Results ① There were 275 intersection targets between peiminine and colon cancer.Molecular docking showed that peiminine could stably dock and interact with the protein structures of AKT1,EGFR,HSP90AA1 and SRC:Peiminine interacted with the amino acid residues of AKT1 mainly through hydrogen bonding.Peiminine interacted with amino acid residues of EGFR,HSP90AA1 and SRC mainly through ionic bond and hydrogen bonding.② Phosphoproteomics analysis showed that:Compared with the NC group,880 phosphorylated modification sites were significantly up-regulated in the DT group(including S124 and S126 sites of AKT1 and T648 and S643 sites of EGFR),and 425 phosphorylated modification sites were significantly down-regulated in the DT group(including T317 sites of HSP90AA1).③ Comparing the results of network pharmacology and phosphoproteomics analysis,it was found that:The main targets of Peiminine against Colon cancer are AKT1,EGFR and HSP90AA1.It promotes apoptosis of colon cancer cells by regulating 17 pathways including AMPK signaling pathway,mTOR signaling pathway and Choline metabolism in cancer.Conclusion This study revealed the potential mechanism of peiminine in the treatment of colon cancer with multiple targets and multiple pathways,and provided a certain direction and reference for subsequent research.

The approach of stem cell transplantation to treat different types of diseases has been becoming a hot issue in the field of regenerative medicine.Potentially, bone marrow mesenchymal stem cells (BMMSCs) possess strong proliferation and multi-di-rection differentiation ability .Under special certain kind of induction circumstances , BMMSCs can trans-differentiate into myocardial cells, osteoblasts, fat cells, neurons, endothelial cells.It is difficult that how to obtain enough BMMSCs for basic and clinical studies using BMMSCs treating diseases .Currently , there are many methods of in vitro proliferation of BMMSCs with advantages and disadvan -tages.In this paper, an overview of BMMSCs proliferation strategy in vitro is provided .

[Abstract ] Objective Liver cirrhosis modeling with carbon tetrachloride (CCL4) may be influenced by many factors, such as drug concentration and dosing methods.In this article, we explored the influences of different concentrations and different dosing methods and time of CCL4 on the induction of liver cirrhosis in rats. Methods We constructed rat models of liver cirrhosis with different con-centrations of CCL4(30%and 50%), using different dosing methods (subcutaneous injection, intraperitoneal injection, and intragastric administration) , and for different lengths of dosing time (8 wk, 10 wk, and 12 wk) .We collected blood and liver tissues from the rats at different time points for HE and MTC staining, biochemical and histomorphological scores based on the Scoring Model for Liver Cirrho-sis Disease (SLCD, expressed by R) and the Laennec Fibrosis Scoring System (LFSS, expressed by L), and analysis of the results by 3 ×2 ×3 factorial experiment design. Results The R value was lower in the intraperitoneal injection than in the subcutaneous injection and intragastric administration groups, and so was it in the 50% than in the 30%CCL4 group, decreasing with the extending of dosing time, with statistically significant differences in the main effects ( P<0.05) as well as a remarkable correlation among drug concentrations, dosing methods, and dosing time (P<0.05).The L value was higher in the intraperitoneal injection than in the subcutaneous injection and intra-gastric administration groups, and so was it in the 50% than in the 30% CCL4 group and in the 12 wk than in the 10 wk and 8 wk groups, with statistically significant differences in the main effects ( P<0.05) but no remarkable correlation among drug concentrations, dosing methods, and dosing time ( P>0.05) .The death rate showed an increasing trend in the intraperitoneal injection, subcutaneous injection and intragastric administration of 30% CCL4 (25.33%, 37.78%, and 38.37%) and 50% CCL4 (42.97%, 47.85%, and 51.88%), higher in the 50%than in the 30%CCL4 .However, no significant differences were found in the survival curves among differ-ent dosing methods or between different drug concentrations (P>0.05). Conclusion Intraperitoneal injection was better than subcu-taneous injection and intragastric administration of CCL4 in inducing liver cirrhosis, and the three dosing methods all showed progressively improved efficiency of modeling with the increase of drug concentration and dosing time.

Objective: To compare the difference of infrared radiation temperature on body surface of points between healthy people and patients with hyperplasia of mammary glands. Method: The thermaCATMT P30 infrared thermal imaging system was selected to measure the infrared radiation temperature on body surface of eight points in four meridians in 74 patients with hyperplasia of mammary glands and 63 healthy people. Results: The infrared radiation temperatures on body surface of right Youmen (KI 21) in cases with hyperplasia of mammary glands were significantly higher than the healthy people (P=0.009), and the infrared radiation temperatures on left Youmen (KI 21) and other points had no significant difference with healthy people (P>0.05). Conclusion: The infrared radiation temperatures on body surface of right Youmen (KI 21) have significant differences with healthy people. This might occur because of the pathogenesis of hyperplasia of mammary glands and of the specificity of the point Youmen (KI 21).

Granulocyte colony-stimulating factor(G-CSF) is a specific hematopoietic regulating growth factor of granulocyte lineage.It can be used to treat different kinds of granulocytopenia.Recently a variety of basic and clinical researches reported that G-CSF can mobilize bone marrow stem cells and haemopoietic stem cells in the peripheral blood,which suggested a potential aproach of releasing、enriching、mobolizing、promoting migration and inducing cell differentiation in the stem cell transplantation.The recent application of G-CSF in stem cell transplantation is reviewed in this view.