Objective To analyze the epidemiologic burden of colorectal cancer in Xishan District, Kunming City, Yunnan Province from 2018 to 2020. Methods Indicators of epidemiologic burden were calculated, including incidence rate, mortality rate, age-specific incidence/mortality rates, potential years of life lost (PYLL), and disability-adjusted life years (DALY) based on the National Disease Control and Prevention Center’s "Cancer Information Registration and Reporting System" and "Cause of Death Registration System". Results From 2018 to 2020, the ASR (China) for the incidence of colorectal cancer in Xishan District, Kunming City increased from 25.27/10⁵ to 26.29/10⁵, while the ASR (China) for mortality decreased from 17.11/10⁵ to 16.03/10⁵. The PYLL in 2018–2020 were 1399.63, 1376.78, and 1571.56 person-years, respectively, whereas the DALY in 2018–2020 were 1927.74, 1875.05, and 1945.17 person-years, respectively. The morbidity, mortality, PYLL, and DALY were generally higher in males than in females over 45 years of age. Conclusion Given the parallel trends of persistent incidence risk and steadily declining mortality for colorectal cancer in Xishan District, Kunming City, a three-tier prevention and control system centered on "lifestyle intervention–regular screening–positive case tracking" should be established, with a focus on covering high-risk populations, particularly males over 45 years of age.

Glioblastoma (GBM) is a highly aggressive primary brain tumor characterized by poor prognosis. Conventional chemo-radiotherapy demonstrates limited therapeutic efficacy and is often accompanied by significant side effects, largely due to factors such as drug resistance, radiation resistance, the presence of the blood-brain barrier (BBB), and the activation of DNA damage repair mechanisms. There is a pressing need to enhance treatment efficacy, with BRD4 identified as a promising target for increasing GBM sensitivity to therapy. Lacking small molecule inhibitors, BRD4 can be degraded using PROteolysis Targeting Chimera (PROTAC), thereby inhibiting DNA damage repair. To deliver PROTAC, SIAIS171142 (SIS) effectively, we designed a responsive nanocapsule, MPL_(SS)P@SIS, featuring GBM-targeting and GSH-responsive drug release. Modified with 1-methyl-l-tryptophan (MLT), nanocapsules facilitate targeted delivery of SIS, downregulating BRD4 and sensitizing GBM cells to radiotherapy and chemotherapy. After intravenous administration, MPL_(SS)P@SIS selectively accumulates in tumor tissue, enhancing the effects of radiotherapy and temozolomide (TMZ) by increasing DNA damage and oxidative stress. GSH activates the nanocapsules, triggering BRD4 degradation and hindering DNA repair. In mouse models, the nanosensitizer, combined with TMZ and X-ray irradiation, efficiently inhibited the growth of GBM. These findings demonstrate a novel PROTAC-based sensitization strategy targeting BRD4, offering a promising approach for effective GBM therapy.

Based on the analysis of the relevant records of the contralateral bloodletting technique, it is believed that this needling technique has 3 key characteristics, including "contralateral selection of points", "points on the four extremities dominated" and "collaterals selected rather than meridian points". Through the studies on philology and the annotation by the doctors in the past dynasties, a part of point prescriptions for the contralateral bloodletting is investigated, and those not belonged to the category of this needling technique in Suwen: Miuci Lun (On Contralateral Bloodletting) are distinguished. It is concluded that the failure of consensus-making in classifying point prescriptions is due to the lack of understanding on the characteristics of contralateral bloodletting and the misunderstanding on the errors of sentences and words. It is suggested that the study on theoretic concepts should lay on the problems relevant with the generalization and alienation of concepts. The mode of "combination of the detail with the whole" should be adopted. It starts from grasping the overall by systematic review, and followed by deliberating in details, so as to correctly identify the original concept and connotation.
Humans ; Bloodletting/methods* ; Acupuncture Points ; History, Ancient ; Meridians ; China

Objective To analyze the levels of serum solute carrier family 7 member 11(SLC7A11)and platelet-derived growth factor BB(PDGFBB)in children with bronchial asthma and their correlations with disease severity.Methods A total of 80 children with acute asthma were en-rolled in acute asthma group,and divided into severe asthma group(40 cases)and mild asthma group(40 cases)according to severity of the disease.Additionally,80 children with asthma in re-mission during the same period were enrolled in remission group,and 80 healthy children undergoing physical examination during the same period were enrolled in control group.Enzyme-linked immu-nosorbent assay(ELISA)was used to detect the levels of serum SLC7A11 and PDGFBB in children from each group.Pearson correlation analysis was conducted to assess the correlation between serum levels of SLC7A11 and PDGFBB and lung function indicators[peak expiratory flow(PEF)and forced expiratory volume in one second as percentage of predicted(FEV1％pred)]in asthmatic children.Multivariate Logistic regression analysis was performed to explore the relationships ofn ser-um levels of SLC7A11 and PDGFBB with disease severity in asthmatic children.Receiver operating characteristic(ROC)curves were plotted to evaluate the diagnostic efficacy of serum SLC7A11 and PDGFBB levels for severe asthma.Results The serum SLC7A11 levels and PEF,FEV1％pred in the acute asthma group were lower than those in the remission group and control group,while the PDGFBB levels were higher than those in the remission group and control group(P＜0.05).The serum SLC7A11 levels and PEF,FEV1％pred in the remission group were lower than those in the con-trol group,while the PDGFBB levels were higher than those in the control group(P＜0.05).The ser-um SLC7A11 levels in asthmatic children were positively correlated with PEF and FEV1％pred(P＜0.05),while the serum PDGFBB levels were negatively correlated with PEF and FEV1％pred(P＜0.05).The serum SLC7A11 levels and PEF,FEV1％pred in the severe asthma group were lower than those in the mild asthma group,while the PDGFBB levels were higher than those in the mild asthma group(P＜0.05).High PEF,high FEV1％pred,and high SLC7A11 were independent protective factors for severe asthma(P＜0.05),while high PDGFBB was an independent risk factor(P＜0.05).The area under the ROC curve for the combined diagnosis of severe asthma using ser-um SLC7A11 and PDGFBB levels was 0.851,significantly larger than the areas under the ROC curves for the individual diagnosis using SLC7A11(0.790)and PDGFBB(0.785)alone(P＜0.05).Conclusion Decreased serum SLC7A11 levels and increased serum PDGFBB levels in asthmatic children are closely related to decreased lung function and increased disease severity.The combined detection of these two biomarkers has high diagnostic value for severe asthma.

OBJECTIVE To observe the clinical efficacy of Gandou Fumu Decoction(GDFMD)combined with swallowing func-tion training on Wilson's disease with dysphagia of phlegm stasis type.METHODS Sixty-eight WD patients in The First Affiliated Hospital of Anhui University of Chinese Medicine were randomly equally divided into a control group(34 cases)and a treatment group(34 cases)during October 2021 to October 2024.Control group patients were treated with basic therapy such as copper drainage and swallowing training,while patients in the treatment group received additional GDFMD.The scores of traditional Chinese medicine(TCM)syndrome scores,Water Swallow Test(WST),Standardized Swallowing Assessment(SSA),Functional Oral Intake Scale(FOIS),Barthel Scale were observed before and after the treatment.24-hour urinary copper,superoxide dismutase(SOD)and malon-dialdehyde(MDA)levels were measured in both groups.RESULTS After treatment,the TCM syndrome scores,SSA,WST scale scores and blood MDA levels of the two groups of patients were significantly reduced(P<0.05,P<0.01),and the treatment group was better than the control group(P<0.05);FOIS,Barthel scale scores,24-hour urine copper content and serum SOD level in both groups were significantly increased(P<0.05,P<0.01),and the treatment group was better than the control group(P<0.05).CON-CLUSION The combined therapy of Gandou Fumu Decoction with swallowing training can improve the swallowing function on Wil-son's disease with dysphagia of phlegm stasis type and enhance the copper removing.The mechanism may be related to improving the level of antioxidant stress.

Objective To explore the effect of the pneumatic and electric hand training system with multiple sensory stimulation mo-dalities combined with low-frequency repetitive transcranial magnetic stimulation on hand movement and tactile pressure sensation in patients with stroke.Methods From May,2023 to October,2024,62 stroke patients from the First Affiliated Hospital of University of South China were randomly divided into control group(n=31)and experimental group(n=31).Both groups received conventional rehabilitation,and 1 Hz low-frequency repetitive transcranial magnetic stimulation in the primary cerebral motor cortex on the unaffected side,while the experimental group received the pneumatic and electric hand training system with multiple sensory stimulation modalities in addition,for four weeks.They were as-sessed with Fugl-Meyer Assessment-Upper Extremities(FMA-UE),Motor Assessment Scale(MAS),Semmes Weinstein Monofilament Test(SWMT)and modified Barthel index(MBI)before and after treatment.Results One case dropped out in control group and one in the experimental group.After treatment,the scores of FMA-UE,MAS,SWMT and MBI increased in both groups(|t|>5.464,P<0.001),expect the score of SWMT in the control group(P>0.05),and all scores were better in the experimental group than in the control group(|t|>2.195,P<0.05).Conclusion The pneumatic and electric hand training system with multiple sensory stimulation modalities combined with low-frequency repetitive transcranial magnetic stimulation can improve the motor and tactile pressure func-tion of stroke patients,and improve their activity of daily living.

OBJECTIVE To observe the clinical efficacy of Gandou Fumu Decoction(GDFMD)combined with swallowing func-tion training on Wilson's disease with dysphagia of phlegm stasis type.METHODS Sixty-eight WD patients in The First Affiliated Hospital of Anhui University of Chinese Medicine were randomly equally divided into a control group(34 cases)and a treatment group(34 cases)during October 2021 to October 2024.Control group patients were treated with basic therapy such as copper drainage and swallowing training,while patients in the treatment group received additional GDFMD.The scores of traditional Chinese medicine(TCM)syndrome scores,Water Swallow Test(WST),Standardized Swallowing Assessment(SSA),Functional Oral Intake Scale(FOIS),Barthel Scale were observed before and after the treatment.24-hour urinary copper,superoxide dismutase(SOD)and malon-dialdehyde(MDA)levels were measured in both groups.RESULTS After treatment,the TCM syndrome scores,SSA,WST scale scores and blood MDA levels of the two groups of patients were significantly reduced(P<0.05,P<0.01),and the treatment group was better than the control group(P<0.05);FOIS,Barthel scale scores,24-hour urine copper content and serum SOD level in both groups were significantly increased(P<0.05,P<0.01),and the treatment group was better than the control group(P<0.05).CON-CLUSION The combined therapy of Gandou Fumu Decoction with swallowing training can improve the swallowing function on Wil-son's disease with dysphagia of phlegm stasis type and enhance the copper removing.The mechanism may be related to improving the level of antioxidant stress.

Objective To explore the effect of the pneumatic and electric hand training system with multiple sensory stimulation mo-dalities combined with low-frequency repetitive transcranial magnetic stimulation on hand movement and tactile pressure sensation in patients with stroke.Methods From May,2023 to October,2024,62 stroke patients from the First Affiliated Hospital of University of South China were randomly divided into control group(n=31)and experimental group(n=31).Both groups received conventional rehabilitation,and 1 Hz low-frequency repetitive transcranial magnetic stimulation in the primary cerebral motor cortex on the unaffected side,while the experimental group received the pneumatic and electric hand training system with multiple sensory stimulation modalities in addition,for four weeks.They were as-sessed with Fugl-Meyer Assessment-Upper Extremities(FMA-UE),Motor Assessment Scale(MAS),Semmes Weinstein Monofilament Test(SWMT)and modified Barthel index(MBI)before and after treatment.Results One case dropped out in control group and one in the experimental group.After treatment,the scores of FMA-UE,MAS,SWMT and MBI increased in both groups(|t|>5.464,P<0.001),expect the score of SWMT in the control group(P>0.05),and all scores were better in the experimental group than in the control group(|t|>2.195,P<0.05).Conclusion The pneumatic and electric hand training system with multiple sensory stimulation modalities combined with low-frequency repetitive transcranial magnetic stimulation can improve the motor and tactile pressure func-tion of stroke patients,and improve their activity of daily living.

ObjectiveTo investigate the effect of total glucosides of paeony （TGP） on neurotoxicity induced by aqueous extract of Strychni Semen （SA） in mice and to explore its mechanism. MethodThirty-two male KM mice were randomly divided into normal group，SA group （19.5 mg·kg^-1），TGP group （225 mg·kg^-1），and SA+TGP group （SA 19.5 mg·kg^-1+TGP 225 mg·kg^-1）. The open field test and beam walking test were used to observe the behavioral changes in mice. Pathological changes in the Nissl bodies of the cerebral cortex were assessed through Nissl staining. The levels of malondialdehyde （MDA），glutamate （Glu） in the mouse brain tissue，and serum levels of 5-hydroxytryptamine （5-HT） were detected using enzyme-linked immunosorbent assay （ELISA）. Transcriptome sequencing was employed to analyze gene expression profiles in the brain tissue. Common differentially expressed genes （DEGs） underwent gene ontology （GO） and kyoto encyclopedia of genes and genomes （KEGG） enrichment analyses. The mRNA expression levels of key targets were determined using quantitative real-time polymerase chain reaction （Real-time PCR）. ResultCompared with the normal group，the SA group exhibited significant increases in side-to-side distance and average speed in the open field test，as well as increased walking time on the balance beam. The axons of cortical neurons were absent，and the levels of Glu and MDA in the brain tissue were significantly elevated （P<0.05，P<0.01），along with a notable increase in serum 5-HT levels （P<0.05）. In contrast to the SA group，the SA+TGP group significantly reduced the side-to-side distance，average speed，and balance beam walking time （P<0.05 or P<0.01）. The neuronal axons were clearly visible，and levels of 5-HT，Glu，and MDA were decreased （P<0.05，P<0.01）. Transcriptome analysis indicated that TGP could regulate the glutamate receptor，ionotropic，N-methyl-D-aspartate 2a （GRIN2A）/phospholipase C β₁ （PLCB1）/protein kinase C，gamma （PRKCG） signaling pathway. Compared with the normal group，SA significantly decreased the expression of GRIN2A，PLCB1，and PRKCG genes in the mouse brain （P<0.01），while the mRNA levels of GRIN2A and PRKCG significantly increased after TGP administration （P<0.05，P<0.01）. ConclusionSA induces significant neurotoxicity in the mouse brain，and TGP significantly alleviates SA-induced neurological damage，potentially through the GRIN2A/PLCB1/PRKCG signaling pathway.

Hepatic fibrosis is a pathological process of chronic liver injury. Without timely intervention and treatment， liver fibrosis may eventually lead to liver cirrhosis and cancer. Janus kinase （JAK）/signal transducer and activator of transcription （STAT） signaling pathway is closely associated with the occurrence and development of liver fibrosis. Based on this， this paper summarized and analyzed the mechanism and effects of active ingredients and compounds of traditional Chinese medicine improving liver fibrosis based on JAK/STAT signaling pathway. It is found that the active ingredients and compounds of traditional Chinese medicine that promote blood circulation and remove blood stasis （ingredients such as ethanol extract of Euonymus alatus and paclitaxel， as well as compounds such as Ershiwuwei songshi pill and Ganfukang）， clear away heat and toxic material （ingredients such as betulinic acid， total flavonoids from Persicaria perfoliata， as well as compounds such as Pianzaihuang and Kehuang capsules）， and sooth the liver and promote qi circulation （ingredients such as fraxetin and cucurbitacin B， as well as compounds such as Chaihu shugan powder and Xiaochaihu decoction） can all relieve liver fibrosis by inhibiting the activity of the JAK/STAT signaling pathway， reducing inflammatory reactions， and inhibiting the proliferation of hepatic stellate cells.