In fixed prosthodontics, clear exposure of the preparation margin is the prerequisite for obtaining accurate digital impressions and improving the marginal fit of restorations. To resolve the issues associated with the cord retraction technique, such as pain, acute injury, and prolonged procedural time, this study proposes a new technology for intraoral digital impression taking with pneumatic gingival retraction. The new scanning head blows a high-speed airflow that instantaneously separates the free gingiva, locally exposing the subgingival preparation margin. Combined with the farthest point preservation stitching algorithm based on the distance from the normal vector and high-speed laser scanning photography, it achieves global preparation edge data and gingival reconstruction, realizing painless, non-invasive, and efficient precise acquisition of the preparation margin. Using this new technique, a patient with a full porcelain crown restoration on a posterior tooth was treated. The digital impression revealed a clear margin of the preparation, and the crown made from this data has a good marginal fit.

Objective:To observe the changes of macular structure and microvessels in eyes with diabetes macular ischemia (DMI).Methods:A retrospective case study. From January 2023 to July 2023, 23 patients of 31 eyes diagnosed with DMI at Tangshan Ophthalmological Hospital were included in this study. Among them, there were 14 males with 23 eyes; Female cases with 8 eyes. Age were (59.5±4.6) years old. According to the DMI grading standard formulated by the research group for early treatment of diabetes retinopathy, the patients were divided into mild DMI group, moderate DMI group, and severe DMI group, with 8, 12, and 11 eyes respectively. The blood flow density (VD), perfusion area (FA), small vessel VD (SVD), inner retinal capillary plexus VD, FA, and outer retinal, choroidal, and ganglion cell complex (GCC) thickness within 1 mm of the macular fovea in retinal superficial vascular plexus (SVP)were measured using a scanning frequency light source optical coherence tomography instrument. The changes in macular structure and microvasculature in the affected eyes of different degrees of DMI groups were compared and observed. Inter group comparisons were conducted using one-way ANOVA or Kruskal Wallis H-test. Spearman correlation analysis was used to analyze the correlation between DMI severity and GCC, outer retina, choroid thickness, VD, FA and SVP VD, SVD and FA in inner retina. Results:The GCC ( F=70.670), outer retinal thickness ( H=12.393), VD ( F=105.506), SVD ( H=25.300), FA ( F=107.655), and VD ( H=24.098) and FA ( H=25.300) of the retinal SVP in the mild, moderate, and severe DMI groups were compared, and the differences were statistically significant ( P<0.05). There was no statistically significant difference in choroidal thickness ( H=2.441, P>0.05). Pairwise comparison between groups: VD, SVD, FA of GCC thickness and SVP, and VD of inner retina were statistically significant between severe DMI group and moderate DMI group, and between moderate DMI group and mild DMI group ( P<0.05). The thickness of outer retina was statistically significant between severe DMI group and moderate DMI group ( P<0.05). Inner retinal FA: there were statistically significant differences between severe DMI group, moderate DMI group and mild DMI group ( P<0.05). The correlation analysis results showed that GCC ( r s=-0.918), outer retinal thickness ( r s=-0.448), and inner retinal VD ( r s=-0.894) and FA ( r s=-0.918), as well as VD ( r s=-0.919), SVD ( r s=-0.924), and FA ( r s=-0.939) of retinal SVP, were all negatively correlated with the degree of DMI ( P<0.05). There was no correlation between choroidal thickness and degree of DMI ( r s=-0.081, P>0.05). Conclusion:The thickness of GCC, outer retina and choroid, the VD, SVD, and FA of the retinal SVP, the VD and FA of inner retina are all reduced in eyes with different degrees of DMI, while all of them are negatively correlated with the degree of DMI, except for choroid thickness.

OBJECTIVE To investigate the predictive value of prognostic nutritional index(PNI) and lymphocyte-monocyte ratio(LMR) in severe radiotherapy-induced oral mucositis(RIOM) during treatment of patients with head and neck cancer,and to construct a risk prediction model and test the prediction effect. METHODS A total of 502 patients with head and neck cancer who underwent radiotherapy were recruited from September 2021 to October 2023 in Xiangya Hospital Central South University. The participants were randomly divided into training group and validation group at a ratio of 7:3. According to whether severe RIOM occurred,they were divided into severe RIOM group and non-severe RIOM group. Univariate analysis and logistic regression analysis were used to screen the risk factors of severe RIOM. The receiver operating characteristic(ROC) curve was used to evaluate its prediction effect and R4.3.2 software was used to draw nomograms and decision curve. RESULTS The risk prediction model for patients with head and neck cancer during treatment had five factors,including the number of comorbidities(OR=2.221,95％CI=1.185-4.165),surgical history(OR=2.938,95％CI=1.393-6.198),the degree of tumor differentiation(OR=1.511,95％CI=1.090-2.094),PNI(OR=0.892,95％CI=0.852-0.934),LMR(OR=0.512,95％CI=0.254-1.030). Model formula:Y=2.102+0.413×degree of differentiation+0.798×number of comorbidities+1.078×surgical history-0.114×PNI-0.669×LMR. The validation results of the prediction model showed that the area under the ROC curve of the training group was 0.847(P<0.001),the area under the curve of the validation group was 0.808(P<0.001),and the P values of the Hosmer-Lemeshow test of the modeling group and the validation group were both greater than 0.05. The decision curve was above the reference line within most of the high-risk thresholds. CONCLUSION The risk prediction model constructed in this study has good effect,which can predict the risk of severe RIOM during radiotherapy in patients with head and neck cancer,providing the reference for taking preventive intervention measures for high-risk patients.

Objective To investigate the prevalence and severity of symptoms and to construct symptom networks in head and neck cancer patients during treatment to identify core symptoms and symptom clusters.Methods 366 patients who were hospitalized in 3 tertiary hospitals in Changsha were selected using convenience sampling from March to October 2022 and asked to complete the M.D.Anderson Symptom Inventory-Head & Neck.Exploratory factors analysis was used to extract the symptom clusters,and R packages were used to construct the symptom severity network and symptom clusters network.The centrality indexes of the networks,including strength,closeness,and betweenness,were analyzed to identify core symptoms and core symptom cluster.Results The most common symptoms in head and neck cancer patients during treatment were dry mouth(93.44％),fatigue(89.07％),loss of appetite(86.34％),and difficulty swallowing or chewing(85.79％),and the most severe symptoms were dry mouth,loss of appetite,oral or pharyngeal mucus,and difficulty swallowing or chewing.4 symptom clusters were extracted,namely oral-pharyngeal,gastrointestinal,emotional-sleep,and sickness-sensing behavioral,which could explain 67.415％of the total variance.In the symptom severity network,oral or pharyngeal mucus(rs=9.60)was a symptom with the highest strength.In the symptom clusters network,oral or pharyngeal mucus(rs=1.20),nausea(rs=1.00),fatigue(rs=1.10),and drowsiness(rs=0.97)were the symptoms with the highest strength across 4 symptom clusters.Conclusion Oral or pharyngeal mucus,nausea,fatigue,and drowsiness are the core symptoms of symptom clusters in head and neck cancer patients during treatment.Oral-pharyngeal symptom cluster is the core symptom cluster.It is recommended that clinical staff should develop interventions based on the core symptoms and symptom cluster to implement precise symptom management and improve symptom management efficiency.

Objective:To explore the change characteristics of event-related potential P300 in different violence risk levels of schizophrenic patients and analyze the risk factors of violence in schizophrenic patients.Methods:Totally 158 schizophrenic patients in Lyuzhou hospital of Shihezi City from January 2019 to August 2020 were collected and assessed with the violence risk scale for 3 days.According to the assessment results, the patients who met the inclusion criteria were divided into low-risk group( n=78), medium-risk group( n=51) and high-risk group( n=29). The auditory P300 of patients in each group was completed within 3 days and act of violence was observed and recorded within one week.Data analysis was carried out by SPSS 20.0 software.The changes of P300 in different violence risk groups were analyzed by ANOVA, and the influencing factors of violence in patients with schizophrenia were analyzed by logistic regression. Results:There was no significant difference in latency of P300 among the three groups (χ 2=4.71, P=0.10), but there was significant difference in amplitude of P300( F=6.67, P<0.01). Compared with the low-risk group ((12.14±9.19) μV), the amplitude of P300 in medium-risk group ((8.25±7.13) μV) and high risk group ((6.71±4.97) μV) decreased significantly ( t=-3.14, -5.45, both P<0.05). There was no significant difference in amplitude of P300 between the high-risk group and the middle-risk group( t=-2.31, P>0.05). The latency and amplitude of schizophrenia patients with violent behavior were significantly different from those without violent behavior ( Z=-6.30, 9.78, both P<0.01). High BVC grade (compared with high-risk group, low-risk group: OR=0.03, 95% CI : 0.00-0.35; the middle risk group: OR=0.09, 95% CI : 0.01-0.62), prolonged P300 latency ( OR=1.30, 95% CI : 1.13-1.48) and decreased P300 amplitude ( OR=0.50, 95% CI: 0.36-0.70), delusion of victimization ( OR=0.12, 95% CI: 0.02-0.76)were the risk factors for violent behavior. Conclusion:The latency and amplitude of P300 can be used as the reliable neuroelectrophysiological indicators for evaluating violence risk in patients with schizophrenia.It has important clinical application value for evaluating violence in patients with schizophrenia.

Objective:To find a suitable semen treatment method in in vitro fertilization (IVF). Methods:A prospective randomized double-blind design was performed, 300 infertile couples with female factors and no abnormal fertility test were selected for IVF-embryo transfer (ET), the patients were randomly divided into float group (FY group, n=100), density gradient group (DG group, n=100) and upstream group (SU group, n=100). The remaining sperm after fertilization were collected from three groups, and sections of electron microscope were prepared. The ultrastructure of sperm was observed by transmission electron microscope. The differences among the three groups of selected sperm DNA fragment index (DFI) and fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate during IVF-ET were compared. Results:The sperm DFI of the three groups after treatment was statistically different ( P=0.02), and the sperm DFI of the FY group was significantly lower than that of DG group and SU group (3.22%±2.73% vs. 8.31%±2.14% vs. 6.43%±2.56%). The rates of sperm head and tail plasma membrane integrity rate in FY group were significantly higher than those in the other two groups (92.0%±24.2% vs. 80.2%±29.5% vs. 73.2%±30.1% and 93.9%±1.2% vs. 80.1%±1.1% vs. 74.9%±1.2%), and the differences were statistically significant ( P=0.01, P=0.03). The high-quality embryo rate and the blastocyst formation rate of FY group were significantly higher than those of DG group and SU group [44.14% (452/1024) vs. 32.30% (292/904) vs. 32.46% (296/912) and 54.40% (396/728) vs. 43.52% (302/694) vs. 46.34% (330/712)], and the differences were statistically significant (all P<0.001). The pregnancy rate and the implantation rate in the fresh cycle FY group were significantly higher than those in DG and SU groups [57.14% (24/42) vs. 33.33% (16/48) vs. 35.56% (16/45) and 50.00% (30/60) vs. 27.45% (14/51) vs. 28.26% (13/46)], and the differences were statistically significant ( P=0.04, P=0.02). The pregnancy rate and implantation rate in the resuscitation cycle FY group were significantly higher than those in the DG and SU groups [52.38% (22/42) vs. 31.25% (10/32) vs. 37.14% (13/35) and 52.38% (22/42) vs. 29.41% (10/34) vs. 30.56% (11/36)], and the difference was statistically significant ( P=0.03, P=0.02). Conclusion:The Plankton method can reduce physical damage to sperm, improve the integrity rate of sperm plasma membrane and decrease sperm DFI, improve assisted reproductive technology outcome.

According to the statistics of new malignant tumors in China in 2015, there were 38 800 female patients under 30 years old, who urgently needed doctors' advices and suggestions on fertility protection and preservation. Ovarian tissue freezing method is the best way to preserve female endocrine function and fertility. At present, the key to the survival of ovarian tissue is to solve the problem of ischemia and reperfusion after ovarian transplantation. In this paper, we will systematically review the mechanism of ischemia and reperfusion after ovarian transplantation, as well as some intervention methods including antioxidants, angiogenesis and remote ischemic preconditioning. This will promote the combination of the latest biotechnology and medical technology, develop more effective, safer and operable solutions to improve the ischemic state after ovarian transplantation, and strive for early clinical use to address the increasing need for female fertility preservation.

Objective:To find a suitable semen treatment method in in vitro fertilization (IVF). Methods:A prospective randomized double-blind design was performed, 300 infertile couples with female factors and no abnormal fertility test were selected for IVF-embryo transfer (ET), the patients were randomly divided into float group (FY group, n=100), density gradient group (DG group, n=100) and upstream group (SU group, n=100). The remaining sperm after fertilization were collected from three groups, and sections of electron microscope were prepared. The ultrastructure of sperm was observed by transmission electron microscope. The differences among the three groups of selected sperm DNA fragment index (DFI) and fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate during IVF-ET were compared. Results:The sperm DFI of the three groups after treatment was statistically different ( P=0.02), and the sperm DFI of the FY group was significantly lower than that of DG group and SU group (3.22%±2.73% vs. 8.31%±2.14% vs. 6.43%±2.56%). The rates of sperm head and tail plasma membrane integrity rate in FY group were significantly higher than those in the other two groups (92.0%±24.2% vs. 80.2%±29.5% vs. 73.2%±30.1% and 93.9%±1.2% vs. 80.1%±1.1% vs. 74.9%±1.2%), and the differences were statistically significant ( P=0.01, P=0.03). The high-quality embryo rate and the blastocyst formation rate of FY group were significantly higher than those of DG group and SU group [44.14% (452/1024) vs. 32.30% (292/904) vs. 32.46% (296/912) and 54.40% (396/728) vs. 43.52% (302/694) vs. 46.34% (330/712)], and the differences were statistically significant (all P<0.001). The pregnancy rate and the implantation rate in the fresh cycle FY group were significantly higher than those in DG and SU groups [57.14% (24/42) vs. 33.33% (16/48) vs. 35.56% (16/45) and 50.00% (30/60) vs. 27.45% (14/51) vs. 28.26% (13/46)], and the differences were statistically significant ( P=0.04, P=0.02). The pregnancy rate and implantation rate in the resuscitation cycle FY group were significantly higher than those in the DG and SU groups [52.38% (22/42) vs. 31.25% (10/32) vs. 37.14% (13/35) and 52.38% (22/42) vs. 29.41% (10/34) vs. 30.56% (11/36)], and the difference was statistically significant ( P=0.03, P=0.02). Conclusion:The Plankton method can reduce physical damage to sperm, improve the integrity rate of sperm plasma membrane and decrease sperm DFI, improve assisted reproductive technology outcome.

According to the statistics of new malignant tumors in China in 2015, there were 38 800 female patients under 30 years old, who urgently needed doctors' advices and suggestions on fertility protection and preservation. Ovarian tissue freezing method is the best way to preserve female endocrine function and fertility. At present, the key to the survival of ovarian tissue is to solve the problem of ischemia and reperfusion after ovarian transplantation. In this paper, we will systematically review the mechanism of ischemia and reperfusion after ovarian transplantation, as well as some intervention methods including antioxidants, angiogenesis and remote ischemic preconditioning. This will promote the combination of the latest biotechnology and medical technology, develop more effective, safer and operable solutions to improve the ischemic state after ovarian transplantation, and strive for early clinical use to address the increasing need for female fertility preservation.

Objective To explore the role of forkhead box F2 (FoxF2) in the extracellular matrix of trabecular meshwork.Methods The cultured human trabecular meshwork cells (HTMCs) were divided into Scramble control group and FoxF2 small hairpin RNA (shRNA) group,then FoxF2 shRNA,the FoxF2 restructuring interference carrier was built,HTMCs were infected with FoxF2 shRNA lentivirus.Western blot assay was used to detect the expression of FoxF2 protein and extracellular matrix.Furthermore,Transwell counting experiment was used to analyze the migration ability of HTMCs.Results The cultured HTMCs grew well and showed a long spindle shape.The growth status of HTMCs was well,and their morphological characteristics were consistent with the HTMCs in vivo.The relative expression level of FoxF2 protein in the FoxF2 shRNA group was lower than that in the Scramble control group,with a significant difference between them (0.72 ± 0.02 vs.1.27 ± 0.05;t =16.68,P ＜ 0.01).The relative expression level of fibronectin (FN),collagen type Ⅰ (COL Ⅰ) and α-smooth muscle actin (α-SMA) were 0.43±0.03,0.53 ±0.08 and O.86±0.15 in the FoxF2 shRNA group,and 0.87±0.04,1.66±0.06 and 1.73 ±0.13 in the Scramble control group,respectively,the relative expression levels of FN,COL Ⅰ and α-SMA in the FoxF2 shRNA group were significantly lower than those in the Scramble control group (t =15.08,18.81,7.50,all at P＜0.01).The migration number of HTMCs in the FoxF2 shRNA group was significantly lower than that in the Scramble control group (117.30±11.41 vs.251.00±10.37;t =8.72,P＜0.01).Conclusions The FoxF2 shRNA lentivirus are successfully constructed,which can decrease the expression of FoxF2 in HTMCs.Low expression of FoxF2 can reduce the expression level of extracellular matrix protein in HTMCs and inhibit the migration ability of HTMCs.