Objective: This study aims to explore the role of SH2D3A in cervical cancer, as well as its potential interaction with human papillomavirus (HPV) E7 and microRNA (miRNA). Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were used to compare the expressions of SH2D3A in tissues.To assess the effects of SH2D3A on cervical cancer cell phenotypes, SH2D3A was knocked down in SiHa and HeLa cells, followed by cell proliferation (Cell Counting Kit-8 assay), apoptosis (flow cytometry), and invasion (Transwell assay) analyses. A transplantation tumor model was established to compare the tumorigenic ability of cervical cancer cells before and after SH2D3A silencing. Bioinformatics analysis predicted and dual-luciferase reporter assays verified the sponge adsorption effect of SH2D3A on miRNA. Western blot and qRT-PCR analyses were conducted to examine the impact on target genes following the downregulation of HPV E7 and SH2D3A. Results: SH2D3A expression was significantly elevated in cervical cancer tissues. SH2D3A silencing inhibited cell proliferation and invasion, induced apoptosis, and reduced tumorigenesis in nude mice. Bioinformatics tools identified a binding relationship between SH2D3A and miR-143-3p, confirmed by the luciferase reporter assays. Western blot analysis revealed that SH2D3A knockdown led to decreased levels of Janus kinase 1 (JAK1) and signal transducer and activator of transcription 3 (STAT3) proteins. Additionally, qRT-PCR showed that SH2D3A mRNA levels decreased after HPV E7 silencing, whereas miR-143-3p levels significantly increased. Conclusion HPV E7 influences SH2D3A expression through miR-143-3p, thereby regulating the JAK1/STAT3 pathway. This mechanism promotes the occurrence and development of cervical cancer.

Objective: To investigate the interest and awareness of traditional Chinese medicine undergraduates on the course of Chinese medical literature, and to provide suggestions for further enhancement of undergraduates' cognition and in-depth study of this subject. Methods: Taking Shandong University of Traditional Chinese Medicine as an example, 726 undergraduates were sampled and randomly sampled by stratified sampling, and the interest and awareness of Chinese medical literature were suveyed and summarized, and the factors that led to this situation were analyzed. Results: A total of 119 (16.4%) knew and took the course, 78 (10.7%) said they often read Chinese medical literature on their own initiative, 455 (62.7%) said that the factors affecting their access to Chinese medical literature were reading difficulties. 410 (56.5%) students suggested that the course of Chinese medical literature should be open as an optional course. Conclusions The undergraduates have high interest and low awareness of the Chinese medical literature. It is suggested that the school should carry out curriculum reform and organize relevant publicity activities in order to increase the awareness of the undergraduates on the Chinese medical literature.

Objective To investigate the effect of short-term environmental ozone (O3) exposure on hospitalization risk of acute ischemic stroke (AIS) and its subtypes.Methods From January 1,2016 to December 31,2017,the hospitalization data of patients with AIS from Zhongnan Hospital of Wuhan University,air pollutant data published by China Air Quality Online Monitoring and Analysis Plafform,and the meteorological data published by China Meteorological Data Network were collectcd.According to TOAST etiological classification criteria,the patients with AIS were divided into large-artery atherosclerosis (LAA),small-artery occlusion (SVO),cardioembolism (CE),and stroke of other etiology (SOE).The effect of short-term O3 exposure on the hospitalization risk of AIS and its subtypes was analyzed retrospectively using a distributed lag non-linear model of time series analysis.Results A total of 1 413 patients with AIS were enrolled,including 910 males (64.4％),aged 67.7± 12.8 years (range,18-99 years).Short-term O3 exposure increased the overall hospitalization risk of AIS [relative risk (RR) 1.06,95％ confidence interval (CI) 0.99-1.13],mainly caused by increased hospitalization risks of LAA (RR 1.17,95％ CI 1.02-1.34;lag 5 d) and SVO (RR 1.24,95％ CI 1.06-1.45;lag 3 d).After introducing double pollutant (O3 + other pollutants) model its RR did not have significant changes.A stratified analysis based on demographic characteristics and vascular risk factors showed that the different populations had different sensitivities to the acute hazard effects of O3.Conclusion Short-term exposure to O3 could significantly increase the hospitalization risks of LAA and SVO.

Objective To investigate the awareness of AIDS knowledge among medical staffs and their preventive behaviors among hospitals of traditional Chinese Medicine in Liangshan and to analyze the influential factors so as to provide the scientific basis for the staffs' training as well as for the prevention and control about AIDS. Methods Medical staffs from 7 hospitals of traditional Chinese medicine in Liangshan were surveyed using self-designed questionnaires,among which 276 were valid. Results The total score of the medical staffs' knowledge on AIDS was 34.14 ±6.92 points. Those with the highest score accounted for 16.73%, others with intermediate score accounted for 56.13%and still others with the low score accounted for 27.14%. The total score of the medical staffs' preventive behaviors on AIDS was 35.20 ±9.48 points, with the highest score being 12.64%, the intermediate score being 30.86%and the low score being 56.51%. The major factors to influence the medial staffs' knowledge about AIDS related to his/her educational level, specialty, education background of education, trainings on AIDS and contacts with HIV/AIDS patients and so on (<0.05) . The hospital's level, the specialty, whether or not being trained with AIDS knowledge concerned the level of occupational protection ( <0.05), whereas their ages, titles of a technical post, working years of working were irrelevant. Conclusion The overall level of these medical staffs’knowledge and preventive behaviors has not been satisfactory. Therefore, the various training programs will be necessary to improve the technical level of the medical staffs.