Objective To investigate the changes of CD4+,CD8+and B cells in peripheral blood of breast cancer patients detected before and after surgery and following chemotherapy and the relationship with clinical prognosis.Methods Seventy-nine pa-tients with breast cancer who were admitted for treatment in the Department of General Surgery,Shanghai Minhang Central Hospital from July 2015 to March 2017 were enrolled for the study.T cell and B cell counts were detected by flow cytometry in the peripheral blood of breast cancer patients and benign breast cancer patients,and differences were analyzed between the patients of the 2 groups.Then,the relationship between T cell and B cell counts detected in the patients before and after surgery and following chemotherapy and those pa -tients with different TNM staging and molecular typing.Results There were statistical differences in CD4+cell counts and TNM stag-ing between the patients with malignant and benign tumors(P<0.05;P<0.05).There were also statistical differences in CD8+cell counts,when comparisons were made between the patients with malignant and benign tumors, before and after surgery and following chemotherapy,and between different age stages(P<0.05;P<0.05).After chemotherapy, there were statistical differences between different TNM stages(P=0.001)and molecular types(P =0.011).Statistical differences could also be seen in B cells between malignant and benign tumors,between before and after chemotherapy as well as between different age stages(P<0.05;P<0.05;P<0.05).After surgery,statistical differences could also be noted between different TNM stages(P =0.026)and molecular types(P =0.022).CD8+and B cells might be prognostic factors, however, the levels of CD8+and B cells were not necessarily independent predictors of survival after breast cancer treatment.Conclusion The levels of B cells, CD4+and CD8+in peripheral blood have a certain value for the diagnosis and prognosis of breast cancer patients,for this reason,it is worth further research and may promise fu-ture clinical application.

Objective To investigate the effect of different HAART regimens on liver function,viral load and CD4+T cells of HIV infected children with HCV co-infection.Methods A total of 40 patients were divided into 4 groups:13 cases in AZT+3TC+NVP group(group A),16 cases in AZT+3TC+EFV group(group B),5 cases in D4T+3TC+EFV group(group C)and 6 cases in D4T+3TC+NVP group(group D).Liver function,viral load and CD4+T cells of patients in 4 groups were compared between before and after HAART.Results Eight patients(20％)had abnormal liver function before HAART treatment,and all of them had 1 level of liver damage.After receiving HAART,levels of ALT and AST were increased in group A and B(P<0.01);AST levels were increased in group C(P<0.01).CD4+T counts were all increased and HIV viral loads were decreased in 4 groups(P<0.01).There was no significant difference in RNA HCV load before and after HAART treatment(P>0.05).Conclusion Different HAART regimens can increase the liver damage in HIV infected children co-infected with HCV,but have little effect on the treatment efficiency of HAART regimens.

Objective · To prepare the liposomal pemetrexed and investigate its effects on MCF-7 breast cancer cells in vitro and nude mice bearing MCF-7 xenograft tumors. Methods · Liposomal pemetrexed was prepared by film dispersion method. Inhibition of MCF-7 breast cancer cell lines was evaluated by CCK-8 method, and anti-tumor effects were investigated on Balb/c nude mice bearing MCF-7 xenograft tumors. Results · Liposomal pemetrexed inhibited the growth of MCF-7 cells. When the concentrations of pemetrexed were 0.20, 0.40 and 10.00 μg/mL, the cell viability in experiment group (liposomal pemetrexed) was significantly lower than that in control group (pemetrexed of same concentration gradient), with P values of 0.013, 0.035 and 0.041, respectively. Compared with blank group (same volume of PBS), the volumes and weights of tumors of nude mice in experiment group(liposomal pemetrexed) and control group (same volume of pemetrexed) were significantly lower, and the volume and weight of tumor in experiment group were also significantly lower than those in control group (P=0.000). Conclusion · Compared to bulk drug of pemetrexed, liposomal pemetrexed can inhibit the growth of MCF-7 breast cancer cells and the Balb/c nude mice bearing MCF-7 xenograft tumors.

Objective:To investigate the change of liver function,viral load and CD4+T count in pediatric AIDS patients with HBV/HCV co-infection after HARRT therapy,and explore the effect of HBV/HCV co-infection on HAART.Methods:95 pediatric AIDS patients without HBV/HCV co-infection ( group A) ,9 pediatric AIDS patients with HBV co-infection ( group B) and 23 pediatric AIDS patients with HCV co-infection ( group C) who received HAART for 2 year were enrolled.Liver function,viral load and CD4+T count were detected before and after HAART.Results:After HAART for 2 years,26 patients (20.5%) were found with liver injury of grade 2 (1000.05 ) .Conclusion: Co-infection of HBV/HCV can aggravate the liver damage of HIV-1 infected children,but has no significant effect on HAART.