To systematically investigate the capacity of cytomimetic microspheres (CMM) to load different drugs and to evaluate their in vivo behavior, CMM were prepared from 4T1 cells using a freeze-thaw method. The preparation procedure involved freezing in liquid nitrogen for 12 h followed by thawing at 37 °C. Small molecules (doxorubicin and rhodamine B), biomacromolecules (bovine serum albumin and protamine), and nanoparticles (soybean phospholipid-cholesterol liposomes, polyethyleneimine, polyethyleneimine-cinnamaldehyde, and polyethyleneimine-phenylboronic acid) were selected as model drugs. Fluorescence spectrophotometry was employed to evaluate the encapsulation and release capacities of CMM for different types of drugs. The results showed that the drug loadings of CMM for polyethyleneimine-cinnamaldehyde and protamine were (41.09±1.21) and (31.07±0.79) μg/106 cells, respectively, which were significantly higher than those for the other drugs, indicating that CMM are suitable for encapsulating positively charged and hydrophobic drugs. In a mouse model of 4T1 breast cancer lung metastasis, CMM exhibited favorable lung accumulation and inhibited tumor growth. The carrier has a simple preparation procedure and a stable structure, and possesses a certain degree of loading capacity for small molecules, biomacromolecules, and nanoparticles. These findings help deepen the understanding of the drug-loading and release properties of CMM and provide a new strategy for the treatment of tumor lung metastasis.

Soft tissue sarcoma (STS) is a highly heterogeneous group of malignant tumors originating from mesenchymal tissues. The most common sites of STS are limbs (45%), viscera (21%) and retroperitoneum (17%). The incidence of head and neck soft tissue sarcomas (HNSTS) is the lowest (5%) compared with other areas of the body. Due to numerous functional organs and delicate and complex anatomical structures of the head and neck, it is often difficult to perform radical surgical treatment. Therefore, radiotherapy plays an important role in the treatment of HNSTS. Due to its low incidence, radiotherapy for HNSTS has been rarely studied and captivated little attention. In this article, the present situation and clinical evidence of radiotherapy for HNSTS were summarized, aiming to provide reference for clinical practice.

Objective:To analyze the effect of global budget of New Rural Cooperative Medical System (NCMS) on the per-visit inpatient compensations,inpatient compensation ratios,per-visit inpatient out-of-pocket expenses and inpatient out-of-pocket rates.Methods:The difference in difference method was used to control the non-intervention factors and estimate the net impact of global budget.Results:Global budget of NCMS decreased the per-visit inpatient compensations by 14.37 yuan,but it had no statistical significance.The compensation ratio of hospitalization increased by 5.23％,the average hospitalization self-payment decrease by 141.51 yuan,the self-payment decreased by 5.23％,which all had statistical significance while there were differences on the effects for specific diseases.Conclusion:Global budget of NCMS increased the inpatient benefit,but the effect was varies by conditions.In addition,measurement of global budget's standard still needed to be scientific and reasonable.