Childhood simple obesity has become a significant public health issue in China. Modern medicine primarily relies on lifestyle interventions and often suffers from poor long-term compliance， while pharmacological options are limited and associated with potential adverse effects. Traditional Chinese Medicine （TCM） has a long history in the prevention and management of this condition， demonstrating eight distinct advantages， including systematic theoretical foundation， diversified therapeutic approaches， definite therapeutic efficacy， high safety profile， good patient compliance， comprehensive intervention strategies， emphasis on prevention， and stepwise treatment protocols. Additionally， TCM is characterized by six distinctive features： the use of natural medicinal substances， non-invasive external therapies， integration of medicinal dietetics， simple exercise regimens， precise syndrome differentiation， and diverse dosage forms. By combining internal and external treatments， TCM facilitates individualized regimen adjustment and holistic regulation， demonstrating remarkable effects in improving obesity-related metabolic indicators， regulating constitutional imbalance， and promoting healthy behaviors. However， challenges remain， such as inconsistent operational standards， insufficient high-quality clinical evidence， and a gap between basic research and clinical application. Future efforts should focus on accelerating the standardization of TCM diagnosis and treatment， conducting multicenter randomized controlled trials， and fostering interdisciplinary integration， so as to enhance the scientific validity and international recognition of TCM in the prevention and treatment of childhood obesity.

Objective:To construct a multi-dimensional life-cycle management(MDLM)of medical equipment based on fast Fourier transform-convolutional neural network(FFT-CNN),so as to provide data support for the life-cycle management of medical equipment and its multi-dimensional derivation.Methods:The MDLM system of medical equipment was constructed by setting display layer of comprehensive information,data extension layer of multi business,data layer of life cycle of equipment,data acquisition layer.The FFT-CNN algorithm was adopted to mapping characteristic data of operating medical equipment as business data of hospital,and then,they were derived to operational management indicators of hospital from 6 dimensions included carbon trajectory,operation and maintenance trajectory,benefit trajectory,safety trajectory,performance trajectory and assessment trajectory.The data prober of artificial internet of things(AIoT),and pattern recognition and indicator derivation of FFT-CNN were adopted to conduct comprehensively digital transformation management for life-cycle of medical equipment.The management data that MDLM system were applied in the medical equipment of Baoying People's Hospital from August 2023 to April 2024 were selected to compare the efficiency of maintaining magnetic resonance(MR)equipment of hospital before and after the system was applied.Results:The MDLM system of medical equipment realized the life-cycle management for medical equipment in eight stages included demand,procurement,installation,archiving,operation,monitoring,maintenance and scrapping,and realized the use of"the extended data of energy consumption management,operation and maintenance management,efficiency management,safety management,performance management and assessment management that derived from the indicators of 6 dimensions"in conducting routine management for medical equipment of hospital.The average repair time of the failure of MR equipment of hospital has been decreased from 6.8 hours before the system was applied to 2.3 hours after it was applied,which significantly improved the efficiency of operation and maintenance,and the availability efficiency of the equipment.Conclusion:The FFT-CNN-based MDLM system of medical equipment has established special database of life-cycle management for medical equipment,which can provide multi-dimensionally objective,complete and relevant data support for lean management for medical equipment of hospital,and can improve the efficiency of operating and maintaining equipment.

Objective:To investigate the intervention effect and active mechanism of traditional Chinese medicine Marsdenia tenacis-sima on colitis-associated colorectal cancer(CAC).Methods:High-resolution mass spectrometry and a precise mass number data-base were used to identify the chemical components of Marsdenia tenacissima,and network pharmacology methods were used to obtain the main active components and targets of Marsdenia tenacissima in the treatment of colorectal cancer(CRC)and analyze its mecha-nism of action against CRC.A mouse model of CAC was established,and then Marsdenia tenacissima was analyzed in terms of its effect on disease activity index(DAI),body mass,colorectal morphology,and serum levels of tumor necrosis factor-α(TNF-α)and interleu-kin-1β(IL-1β)in CAC mice.Furthermore,16S rDNA sequencing technique was used to assess the impact of Marsdenia tenacissima on the diversity of intestinal microbiota in mice.Results:A total of 50 components were identified by mass spectrometry,and 37 active components were identified by network pharmacology.Furthermore,313 targets related to CRC were identified.A total of 958 GO terms and 173 KEGG terms were obtained,among which inflammation,oxidative stress,and cancer pathways showed a relatively high de-gree of association with Marsdenia tenacissima.After modeling,the mice in each group had the manifestations such as diarrhea,bloody stool,prolapse of anus,and weight loss,and the model group had a significant increase in DAI score after each DSS cycle,while the Marsdenia tenacissima group had a significant reduction in DAI score of the model mice after the first,second,and third cycles(P=0.007,P<0.001,and P<0.001).In addition,compared with the model group,the Marsdenia tenacissima group had a significant in-crease in colorectal length,alleviation of intestinal swelling,a relatively intact structure of epithelial cells,a reduction in inflammatory cell infiltration,a significant increase in goblet cells,a reduction in atypical hyperplasia,and significant reductions in the levels of TNF-α and IL-6 in CAC mice(P<0.001 and 0.002).The Marsdenia tenacissima group had significant reductions in the abundance and diversity of intestinal microbiota,and compared with the model group,the Marsdenia tenacissima group had a significant reduction in the relative abundance of Bacteroides and significant increases in the relative abundances of Muribaculaceae and Clostridia-UCG-014(P=0.002 and 0.009).Conclusion:Marsdenia tenacissima exerts an intervention effect on colorectal cancer in CAC mice,possibly by regulating the pathways associated with inflammation and cancer and altering the diversity of intestinal microbiota.

Objective:To construct a multi-dimensional life-cycle management(MDLM)of medical equipment based on fast Fourier transform-convolutional neural network(FFT-CNN),so as to provide data support for the life-cycle management of medical equipment and its multi-dimensional derivation.Methods:The MDLM system of medical equipment was constructed by setting display layer of comprehensive information,data extension layer of multi business,data layer of life cycle of equipment,data acquisition layer.The FFT-CNN algorithm was adopted to mapping characteristic data of operating medical equipment as business data of hospital,and then,they were derived to operational management indicators of hospital from 6 dimensions included carbon trajectory,operation and maintenance trajectory,benefit trajectory,safety trajectory,performance trajectory and assessment trajectory.The data prober of artificial internet of things(AIoT),and pattern recognition and indicator derivation of FFT-CNN were adopted to conduct comprehensively digital transformation management for life-cycle of medical equipment.The management data that MDLM system were applied in the medical equipment of Baoying People's Hospital from August 2023 to April 2024 were selected to compare the efficiency of maintaining magnetic resonance(MR)equipment of hospital before and after the system was applied.Results:The MDLM system of medical equipment realized the life-cycle management for medical equipment in eight stages included demand,procurement,installation,archiving,operation,monitoring,maintenance and scrapping,and realized the use of"the extended data of energy consumption management,operation and maintenance management,efficiency management,safety management,performance management and assessment management that derived from the indicators of 6 dimensions"in conducting routine management for medical equipment of hospital.The average repair time of the failure of MR equipment of hospital has been decreased from 6.8 hours before the system was applied to 2.3 hours after it was applied,which significantly improved the efficiency of operation and maintenance,and the availability efficiency of the equipment.Conclusion:The FFT-CNN-based MDLM system of medical equipment has established special database of life-cycle management for medical equipment,which can provide multi-dimensionally objective,complete and relevant data support for lean management for medical equipment of hospital,and can improve the efficiency of operating and maintaining equipment.

Metabolic diseases refer to metabolic disorders caused by abnormal material metabolism or energy metabolism of the body such as diabetes,hyperlipidemia,hyperuricemia and liver disease.In recent years,with the change of national diet structure,its incidence has increased year by year,which seriously endangers national physical and mental health.In modern medicine,long-term treatment of metabolic diseases is often used in the treatment of chemical drugs,which is easy to produce drug resistance and liver and kidney damage.Tannin is a class of polyphenolic compounds composed of glucose,gallic acid,flavanols,etc.,which has antibacterial,anti-tumor,anti-oxidation,hypoglycemic,antiviral,anti-inflammatory and analgesic effects,and has a clear easing effect on metabolic diseases.Tannin in the treatment of metabolic diseases has the advantages of less toxic side effects,significant curative effect,low cost,easy to popularize,etc.,which provides a better choice for the treatment of metabolic diseases.

Colorectal cancer is a common malignant tumor.Revealing the interaction between tumor cells and tu-mor microenvironment(TME)can provide new targets for the prevention or therapy of colorectal cancer.Three-dimensional models such as cell spheroids,organoids,organ chips,and three-dimensional printing have been widely used to simulate TME in colorectal cancer,which can better reproduce the structure and heterogeneity of TME.This paper reviews the progress in the application of three-dimensional models in TME research of colorectal cancer to provide references for future research.

Objective:To construct A novel scoring model of immune signatures for colon cancer based on machine learning,which improve the survival prediction and immune therapy.Methods:Screening immune signatures from 1 301 immune-related genes(IRG)by the combined strategy of Lasso+bootstrap+multi Cox to calculate IRG scores of colon cancer patients from TCGA databases,and comprehensive the differences on function,prognostic status and immune therapy between high IRG scores group and IRG scores group.Results:Groups based on IRG scores were significantly different on the prognostic status of colon cancer patients,which were validated by other independent datasets.The IRG scores also could assess the effect of immune therapy of colon cancer.Conclusion:This study provides ideas for immune therapy and researches of colon cancer based on immune genes,and IRG scores can be used to assess the prognosis of colon cancer patients.

As a newly discovered posttranslational modification (PTM), crotonylation has been identified in histone and nonhistone proteins and has important roles in the regulation of various diseases and biological processes. With the development of high-resolution mass spectrometry approaches, an increasing number of endogenous crotonylation has been detected and identified. The discovery of crotonylation regulatory enzymes paved the way for the study of crotonylation mechanism. In this review, we summarize the biological functions and regulatory mechanism of crotonylation in eukaryotes and introduce the recent progress on crotonylation in malignant tumor.

Objective:To investigate the effect and mechanism of lactoferrin (LF) on biological behaviors of brain glioma cells.Methods:The effect of LF at different concentrations (0, 100, 200 and 300 μg/mL) on the proliferative ability of human glioma cell line U87MG was analyzed by MTT assay to screen the optimal drug concentration. U87MG cells were divided into blank control group and LF (200 μg/mL) treatment group. Edu staining, Transwell assay, Mito-Tracker staining and DCFH-DA staining were used to detect the cell proliferation, migration, mitochondrial activity and reactive oxygen level. Autophagy marker protein microtubule associated protein 1 light chain 3B (LC3B) expression was detected by immunofluorescent chemical staining; contents of superoxide dismutase (SOD) and malonaldehyde (MDA) were detected by colorimetric chemical sensors; expressions of genes related to epithel-to-mesenchymal transformation (E-cadherin, fibronectin, N-cadherin, vimentin and Snail) were detected by reverse transcription (RT)-PCR; expressions of apoptosis-related proteins (Bax and Bcl-2) were detected by Western blotting.Results:Compared with blank control group, the LF treatment group had significantly decreased proportions of Edu positive cells and Mito-Tracker positive cells, number of cell migration and SOD content, and statistically increased proportion of DCFH-DA positive cells, MDA content and LC3B immunofluorescent staining intensity in cytoplasm ( P<0.05). Compared with blank control group, the LF treatment group had significantly decreased E-cadherin mRNA expression and Bcl-2 protein expression, and statistically increased fibronectin, N-cadherin, vimentin and Snail mRNA expressions and Bax protein expression ( P<0.05). Conclusion:LF can effectively inhibit the proliferation, migration, invasion and other biological behaviors of glioma cells, whose mechanism is closely related to mitochondrial activity.

BACKGROUND: MicroRNA-20a (miR-20a) is dysregulated in many types of malignancies, including human hepatocellular carcinoma (HCC), but its expression level and functional significance in HCC are still disputed. We aimed to study the role of miR-20a-5p in HCC and its downstream molecular mechanisms. METHODS: We used real-time polymerase chain reaction to detect the expression of miR-20a-5p and runt-related transcription factor 3 ( RUNX3 ) in HCC and paraneoplastic tissue, transfected Huh7 and highly metastatic human hepatocellular carcinoma (MHCC97H) cells. A live cell workstation was used to observe the proliferation and migration of transfected cells. The invasiveness of transfected cells was verified by Transwell assay. Cell apoptosis was detected by flow cytometry. The expression levels of proteins after transfection were measured using simple western immunoblot measurements. Gene expression profiles between HCC and normal samples were obtained from The Cancer Genome Atlas. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment results were processed by the database for annotation, visualization and integrated discovery. Potential target genes of miR-20a-5p were predicted to further investigate how miR-20a-5p regulates epithelial-mesenchymal transition (EMT) in HCC. RESULTS: MiR-20a-5p was significantly highly expressed in HCC tissues, and overexpression of miR-20a-5p significantly promoted HCC cell proliferation, migration, and invasion and inhibited apoptosis in vitro. The protein expression of E-cadherin was decreased and that of vimentin was increased after overexpression of miR-20a-5p in HCC cells. We discovered the intersection of genes from miRDB, miR TarBase, and TargetScan, obtained 397 target genes and finally focused on RUNX3. RUNX3 was not only reduced in HCC specimens but also drastically reduced in HCC cells overexpressing miR-20a-5p. RUNX3 expression decreased with elevated miR-20a-5p, which activated downstream EMT signaling and promoted cell proliferation, migration, and invasion. CONCLUSIONS Since RUNX3 is involved in EMT in HCC, as proven by previous research, our findings provide further evidence for a novel regulatory pathway comprising the miR-20a/RUNX3/EMT axis that upregulates EMT signaling and enhances the migration of HCC cells.
Humans ; Carcinoma, Hepatocellular/pathology* ; Epithelial-Mesenchymal Transition/genetics* ; Liver Neoplasms/pathology* ; Gene Expression Regulation, Neoplastic/genetics* ; MicroRNAs/metabolism* ; Cell Movement/genetics* ; Cell Line, Tumor ; Cell Proliferation/genetics* ; Core Binding Factor Alpha 3 Subunit/metabolism*