Objective Analysing the risk factors for the occurrence of pre-frailty or frailty of the community elderly in Chengdu and constructing a risk prediction model.Methods The general information questionnaire,MNA-SF scale,Changsha version of the MoCA scale,PSQI scale,FRAIL scale,and Traditional Chinese Medicine Constitution Scale for the elderly were used in the cross-sectional survey.A total of 400 elderly people who completed community physical examinations in Chengdu from April 2022 to April 2023 were selected as the research objects.Based on multivariate Logistic regression analysis,the independent influencing factors were determined,and RStudio software was used to construct a risk prediction model nomogram.The physical examination data of 200 elderly people collected in Deyang City from June 2023 to October 2023 were used for external verification.The area under the ROC curve,Hosmer-Lemeshow test and calibration curve,and decision curve were used to evaluate the discrimination,calibration,and clinical practicability of the model.Results Age,abdominal circumference,number of chronic diseases,PSQI score,allergy history,and balanced constitution were independent influencing factors for pre-frailty/frailty in the elderly in the community(P<0.05),and the regression equation was as follows:Logit(P)=0.063×age+0.025×abdominal circumference-1.006×allergy history+0.300×number of chronic diseases+0.082×PSQI-1.013×balanced constitution-8.269.The area under the ROC curve of the modeling group was 0.779(95%CI:0.733-0.825),the sensitivity was 67.1%,the specificity was 76.3%,and the maximum Youden index was 0.434.The area under the ROC curve of the external validation group was 0.783(95%CI:0.709-0.856),the sensitivity was 62.0%,the specificity was 95.0%,and the maximum Youden index was 0.570.The Hosmer-Lemeshow goodness of fit test results of the two groups were χ2=3.285,P=0.915 and χ2=8.376,P=0.398,and the calibration curve fit was good.DCA showed that the threshold probability of clinical benefit was 5%-99%and 21%-98%.Conclusions The pre-frailty/frailty risk prediction model established in this study has good predictive efficacy for the elderly in the community,and the use of this model for screening and early intervention of high-risk populations can be clinically beneficial.

AIM:To investigate the modulatory effects of electroacupuncture(EA)on spinal cord neurons au-tophagy in rats with bone cancer pain.METHODS:(1)Verification of autophagy-related protein expression at different time points in a bone cancer pain model:a total of 56 female Sprague-Dawley(SD)rats were randomly divided into a sham-operated(sham)group and a model group.The model group was further subdivided into 6 subgroups corresponding to time points of 3,6,9,12,15,and 18 d,with 8 rats per subgroup.Thermal and mechanical pain thresholds,tibial bone destruction,and spinal neuron marker neuronal nuclear antigen(NeuN)co-localized with LC3B,Beclin1,and P62 were examined in rats at each designated time point.(2)Changes in spinal autophagy proteins following EA intervention:an additional 40 rats were randomly assigned to sham group,model group,EA group,sham EA(SEA)group,and autoph-agy agonist rapamycin(Rap)group,with 8 animals per group.EA was administered to the rats in EA group beginning on day 6 after modeling,the rats in SEA group received needle insertion without electrical stimulation,while those in Rap group received intraperitoneal injections of rapamycin(5 mg/kg).Thermal pain thresholds were assessed at designated in-tervals,followed by mechanical pain threshold assessments conducted on the subsequent day.Treatment continued until day 21,with rapamycin administered at the end of each intervention day.Tibial bone destruction was evaluated using he-matoxylin-eosin(HE)staining.Expression levels of LC3B Ⅱ/LC3B I,Beclin1,and P62 proteins in the spinal cord were determined by Western blot and immunohistochemistry.RESULTS:(1)Compared with the Sham group,thermal and mechanical pain thresholds were significantly decreased in the model group starting from day 6(P<0.01).Rat tibial bones exhibited notable damage,with severity progressively increasing over time.Protein expression levels of LC3B Ⅱ/LC3B I,Beclin1,and P62 were significantly elevated in the spinal cord at various time points(P<0.01),and these pro-teins were co-localized with spinal cord neurons.(2)Compared with the model group,mechanical and thermal pain thresholds in the EA and Rap groups gradually increased,with statistically significant differences observed from days 8 and 6 onward,respectively(P<0.01).In addition,LC3B Ⅱ/LC3B I and Beclin1 protein expression levels were signifi-cantly upregulated(P<0.01),whereas P62 expression was markedly downregulated(P<0.01).Immunohistochemical analysis demonstrated significantly enhanced positive staining for LC3B Ⅱ/LC3B I and Beclin1 and significantly decreased positive staining for P62 in the spinal cord of rats in the EA and Rap groups(P<0.05).Notably,no significant differences were observed in the SEA group(P>0.05).CONCLUSION:EA promotes spinal cord neurons autophagy in rats with bone cancer pain.The enhancement of autophagy may represent a potential mechanism underlying the analgesic effect of EA in bone cancer pain.

AIM:To investigate the modulatory effects of electroacupuncture(EA)on spinal cord neurons au-tophagy in rats with bone cancer pain.METHODS:(1)Verification of autophagy-related protein expression at different time points in a bone cancer pain model:a total of 56 female Sprague-Dawley(SD)rats were randomly divided into a sham-operated(sham)group and a model group.The model group was further subdivided into 6 subgroups corresponding to time points of 3,6,9,12,15,and 18 d,with 8 rats per subgroup.Thermal and mechanical pain thresholds,tibial bone destruction,and spinal neuron marker neuronal nuclear antigen(NeuN)co-localized with LC3B,Beclin1,and P62 were examined in rats at each designated time point.(2)Changes in spinal autophagy proteins following EA intervention:an additional 40 rats were randomly assigned to sham group,model group,EA group,sham EA(SEA)group,and autoph-agy agonist rapamycin(Rap)group,with 8 animals per group.EA was administered to the rats in EA group beginning on day 6 after modeling,the rats in SEA group received needle insertion without electrical stimulation,while those in Rap group received intraperitoneal injections of rapamycin(5 mg/kg).Thermal pain thresholds were assessed at designated in-tervals,followed by mechanical pain threshold assessments conducted on the subsequent day.Treatment continued until day 21,with rapamycin administered at the end of each intervention day.Tibial bone destruction was evaluated using he-matoxylin-eosin(HE)staining.Expression levels of LC3B Ⅱ/LC3B I,Beclin1,and P62 proteins in the spinal cord were determined by Western blot and immunohistochemistry.RESULTS:(1)Compared with the Sham group,thermal and mechanical pain thresholds were significantly decreased in the model group starting from day 6(P<0.01).Rat tibial bones exhibited notable damage,with severity progressively increasing over time.Protein expression levels of LC3B Ⅱ/LC3B I,Beclin1,and P62 were significantly elevated in the spinal cord at various time points(P<0.01),and these pro-teins were co-localized with spinal cord neurons.(2)Compared with the model group,mechanical and thermal pain thresholds in the EA and Rap groups gradually increased,with statistically significant differences observed from days 8 and 6 onward,respectively(P<0.01).In addition,LC3B Ⅱ/LC3B I and Beclin1 protein expression levels were signifi-cantly upregulated(P<0.01),whereas P62 expression was markedly downregulated(P<0.01).Immunohistochemical analysis demonstrated significantly enhanced positive staining for LC3B Ⅱ/LC3B I and Beclin1 and significantly decreased positive staining for P62 in the spinal cord of rats in the EA and Rap groups(P<0.05).Notably,no significant differences were observed in the SEA group(P>0.05).CONCLUSION:EA promotes spinal cord neurons autophagy in rats with bone cancer pain.The enhancement of autophagy may represent a potential mechanism underlying the analgesic effect of EA in bone cancer pain.

Objective Analysing the risk factors for the occurrence of pre-frailty or frailty of the community elderly in Chengdu and constructing a risk prediction model.Methods The general information questionnaire,MNA-SF scale,Changsha version of the MoCA scale,PSQI scale,FRAIL scale,and Traditional Chinese Medicine Constitution Scale for the elderly were used in the cross-sectional survey.A total of 400 elderly people who completed community physical examinations in Chengdu from April 2022 to April 2023 were selected as the research objects.Based on multivariate Logistic regression analysis,the independent influencing factors were determined,and RStudio software was used to construct a risk prediction model nomogram.The physical examination data of 200 elderly people collected in Deyang City from June 2023 to October 2023 were used for external verification.The area under the ROC curve,Hosmer-Lemeshow test and calibration curve,and decision curve were used to evaluate the discrimination,calibration,and clinical practicability of the model.Results Age,abdominal circumference,number of chronic diseases,PSQI score,allergy history,and balanced constitution were independent influencing factors for pre-frailty/frailty in the elderly in the community(P<0.05),and the regression equation was as follows:Logit(P)=0.063×age+0.025×abdominal circumference-1.006×allergy history+0.300×number of chronic diseases+0.082×PSQI-1.013×balanced constitution-8.269.The area under the ROC curve of the modeling group was 0.779(95%CI:0.733-0.825),the sensitivity was 67.1%,the specificity was 76.3%,and the maximum Youden index was 0.434.The area under the ROC curve of the external validation group was 0.783(95%CI:0.709-0.856),the sensitivity was 62.0%,the specificity was 95.0%,and the maximum Youden index was 0.570.The Hosmer-Lemeshow goodness of fit test results of the two groups were χ2=3.285,P=0.915 and χ2=8.376,P=0.398,and the calibration curve fit was good.DCA showed that the threshold probability of clinical benefit was 5%-99%and 21%-98%.Conclusions The pre-frailty/frailty risk prediction model established in this study has good predictive efficacy for the elderly in the community,and the use of this model for screening and early intervention of high-risk populations can be clinically beneficial.

[Objective]To explore the guiding significance of the abdominal syndrome described in the Fuzheng Qilan(FuKushokiran-Zen) on the clinical application of various kinds of Guishao Decoctions,and explore the corresponding relation of medicine-abdomen syndrome in Fuzheng Qilan(FuKushokiran-Zen),so as to provide reference for the clinical differential use of Guishao Decoctions.[Methods]Sort out the discussion of abdominal syndrome involving Guishao Decoctions,classify and summarize the abdominal syndrome of various kinds of Guishao Decoctions,summarize the basis of abdominal diagnosis of Guizhi(Cinnamomi Ramulus) and Shaoyao(Paeonia lactiflora),and explore the corresponding relationship between abdominal syndrome and the addition and subtraction of Guishao Decoctions.[Results]The Guishao Decoction involved in Fuzheng Qilan(FuKushokiran-Zen) can be divided into warming Yang,nourishing Yin,and nourishing Yin and Yang according to its efficacy,and all parties have their detailed records of abdominal syndrome.Abdominal throbbing and abdominal cramps are the characteristic abdominal syndromes of Guishao Decoctions.Clinically,it can be used based on different abdominal syndromes and their corresponding relationships with prescriptions,with Guizhi Decoction as the base.[Conclusion]As a monograph on abdominal diagnosis of Japanese Kampo medicine,Fuzheng Qilan(FuKushokiran-Zen) has in-depth research on clinical syndrome differentiation,reflecting the diagnosis and treatment characteristics of Kampo doctors "emphasizing practice and neglecting theory".The book inherits the idea of "relative medicine and syndrome" of Yoshitomo Higashito,and specifies "syndrome" as "abdominal syndrome" to guide clinical prescription and medication,which is widely used by later Japanese doctors and worthy of excavation and research.

[Objective]To explore the guiding significance of the abdominal syndrome described in the Fuzheng Qilan(FuKushokiran-Zen) on the clinical application of various kinds of Guishao Decoctions,and explore the corresponding relation of medicine-abdomen syndrome in Fuzheng Qilan(FuKushokiran-Zen),so as to provide reference for the clinical differential use of Guishao Decoctions.[Methods]Sort out the discussion of abdominal syndrome involving Guishao Decoctions,classify and summarize the abdominal syndrome of various kinds of Guishao Decoctions,summarize the basis of abdominal diagnosis of Guizhi(Cinnamomi Ramulus) and Shaoyao(Paeonia lactiflora),and explore the corresponding relationship between abdominal syndrome and the addition and subtraction of Guishao Decoctions.[Results]The Guishao Decoction involved in Fuzheng Qilan(FuKushokiran-Zen) can be divided into warming Yang,nourishing Yin,and nourishing Yin and Yang according to its efficacy,and all parties have their detailed records of abdominal syndrome.Abdominal throbbing and abdominal cramps are the characteristic abdominal syndromes of Guishao Decoctions.Clinically,it can be used based on different abdominal syndromes and their corresponding relationships with prescriptions,with Guizhi Decoction as the base.[Conclusion]As a monograph on abdominal diagnosis of Japanese Kampo medicine,Fuzheng Qilan(FuKushokiran-Zen) has in-depth research on clinical syndrome differentiation,reflecting the diagnosis and treatment characteristics of Kampo doctors "emphasizing practice and neglecting theory".The book inherits the idea of "relative medicine and syndrome" of Yoshitomo Higashito,and specifies "syndrome" as "abdominal syndrome" to guide clinical prescription and medication,which is widely used by later Japanese doctors and worthy of excavation and research.

Presynaptic dopaminergic PET imaging is a useful method for the diagnosis of parkinsonism. Based on the expert consensus on operation and clinical application of dopamine transporter brain PET imaging technology published in 2020, this paper further recommends the relevant elements of result interpretation of presynaptic dopaminergic PET imaging.

ObjectiveTo investigate the social support status and influencing factors of schizophrenics in remission in Northeast Sichuan， and to provide ideas for improving their social support. MethodsFrom May to September 2020， a total of 533 patients who met the diagnosis criteria of the International Classification of Diseases， tenth edition （ICD-10） for schizophrenics in remission at the mental health institutions in Guangyuan， Bazhong and Dazhou cities were selected for the survey， and patients were assessed by self-made demographic and clinical data inventory and Social Support Rating Scale （SSRS）. Then the social support status of schizophrenic in remission and influencing factors were analyzed， meantime， the impact of the second round reimbursement policy of medical insurance benefits on their social support was addressed particularly. Results①The SSRS total score， objective support， subjective support， and utilization of support scores of schizophrenics in remission were lower than those of the national norm （t=5.065~30.382， P<0.01）. ②Univariate analysis showed that SSRS score was relatively high among patients with female gender （t=-3.632）， retired status （F=5.951）， married status （F=5.951）， spouse as primary caregiver （F=23.841）， annual household income >5 000 yuan （F=15.892）， patient's economic income （t=4.083）， and outpatient or online follow-up （F=3.954）， with statistically significant differences （P<0.05 or 0.01）. ③The total and dimensional scores of SSRS in patients with access to the second round medical insurance reimbursement were significantly higher than those without （t=10.195~25.103， P<0.01）. ④Multiple linear regression analysis denoted that gender， work status， marital status， primary caregivers， annual family income， economic income， follow-up visits and the second round medical insurance reimbursement were the factors influencing social support status of schizophrenics in remission （β=0.201~2.115， P<0.05 or 0.01）. ConclusionThe social support of schizophrenics in remission in Northeast Sichuan is below the national average， furthermore， their social support levels are affected by the gender， work status， marital status， primary caregivers， annual family income， economic income， follow-up visits and the second round medical insurance reimbursement， and the second round medical insurance reimbursement may ameliorate the social support status of patients.

Objective:To explore the occurrence and influencing factors of perimenopausal syndrome among Han, Hui and Tibetan women in Gansu Province.Methods:A stratified cluster sampling method was used to select 7290 women aged 40-55 years as the research subjects for cross-sectional survey. The questionnaire was used to investigate demographic characteristics, marriage and childbearing menstrual history, and the modified Kupperman score was used to investigate the symptoms related to perimenopausal syndrome. The data were statistically analyzed using SPSS20.0 software.Results:The natural menopausal age of women in Gansu Province was (47.43±3.99) years old, Hui women had the earliest age of menopause (47.08±4.29). There were statistically significant differences in occupation, education level and residence among the three ethnic groups ( P<0.001, P=0.001, P=0.005). The incidence of perimenopausal syndrome was 58.85% (246/418) for Hui, 44.16% (136/308) for Tibetans, and 41.06% (2695/6564) for Hans. The incidence of perimenopausal syndrome and the grade of related symptoms were significantly different among the three ethnic groups (all P<0.001). Han women had the highest proportion of mild symptoms, while Hui women and Tibetan women had the highest proportion of moderate symptoms. The differences of the incidence of mild and severe symptoms among the three ethnic groups were statistically significant (all P<0.001). Logistic regression analysis showed that engaging in heavy physical labor is a common risk factor for the incidence of perimenopausal syndrome in the three ethnic groups. Conclusion:The prevention and treatment of perimenopausal syndrome can be carried out from the following aspects: promoting economic development, improving living conditions, changing life and work patterns, increasing investment in education, medical and health care, and strengthening knowledge popularization.

Objective:To explore the occurrence and influencing factors of perimenopausal syndrome among Han, Hui and Tibetan women in Gansu Province.Methods:A stratified cluster sampling method was used to select 7290 women aged 40-55 years as the research subjects for cross-sectional survey. The questionnaire was used to investigate demographic characteristics, marriage and childbearing menstrual history, and the modified Kupperman score was used to investigate the symptoms related to perimenopausal syndrome. The data were statistically analyzed using SPSS20.0 software.Results:The natural menopausal age of women in Gansu Province was (47.43±3.99) years old, Hui women had the earliest age of menopause (47.08±4.29). There were statistically significant differences in occupation, education level and residence among the three ethnic groups ( P<0.001, P=0.001, P=0.005). The incidence of perimenopausal syndrome was 58.85% (246/418) for Hui, 44.16% (136/308) for Tibetans, and 41.06% (2695/6564) for Hans. The incidence of perimenopausal syndrome and the grade of related symptoms were significantly different among the three ethnic groups (all P<0.001). Han women had the highest proportion of mild symptoms, while Hui women and Tibetan women had the highest proportion of moderate symptoms. The differences of the incidence of mild and severe symptoms among the three ethnic groups were statistically significant (all P<0.001). Logistic regression analysis showed that engaging in heavy physical labor is a common risk factor for the incidence of perimenopausal syndrome in the three ethnic groups. Conclusion:The prevention and treatment of perimenopausal syndrome can be carried out from the following aspects: promoting economic development, improving living conditions, changing life and work patterns, increasing investment in education, medical and health care, and strengthening knowledge popularization.