Objective: To investigate the role of STAT3 phosphorylation in ferroptosis regulation and its impact on cisplatin resistance mechanisms in human osteosarcoma cells． Methods: Human osteosarcoma HOS cells and cispl- atin-resistant HOS / DDP cells were treated with cisplatin ( 0. 5，1，2，4，8，16，32 mg / L) ，the ferroptosis inducer Erastin ( Era) ( 2 μmol / L) ，and / or the ferroptosis inhibitor Ferrostatin-1 ( Fer1) ( 10 μmol / L) ．Cell viability and proliferation were assessed using the Cell Counting Kit-8 ( CCK-8) and colony formation assays，and cell migration was evaluated via a scratch assay．Reactive oxygen species ( ROS) ，intracellular ferrous iron levels，mitochondrial membrane potential，mitochondrial function，malondialdehyde ( MDA) levels，and the reduced glutathione / oxi- dized glutathione ( GSH / GSSG) ratio were measured using commercial kits．The mRNA expression of ferroptosis- related genes was analyzed by quantitative reverse transcription polymerase chain reaction ( RT-qPCR) ．The protein levels of glutathione peroxidase 4 ( GPX4) ，solute carrier family 7 member 11 ( SLC7A11) ，phosphorylated STAT3 ( p-STAT3) ，and total STAT3 were determined by Western blot． Results: With cisplatin treatment，HOS cells ex- hibited decreased cell viability，mitochondrial membrane potential，and GSH / GSSG ratio ( P＜0. 01) ，along with elevated levels of ROS，ferrous ion，and MDA content ( P ＜0. 01) ． The protein levels of GPX4 ( P ＜0. 01) ， SLC7A11，and p-STAT3 also decreased ( P ＜0. 05) ．Coadministration with the ferroptosis inhibitor Ferrostatin-1 ( Fer-1) reversed these aforementioned effects ( P ＜0. 05) ．In HOS / DDP cells，the mRNA levels of ferroptosis- suppressive genes ( GPX4，FTH1，SLC7A11，and AIFM2) were significantly higher than those in HOS cells ( P < 0. 05) ，whereas the expression of ferroptosis-promoting genes ( ACSL4 and PTGS2) was significantly lower ( P < 0. 05) ．The cisplatin-induced reductions in cell viability and mitochondrial membrane potential，as well as the in- creases in ROS，ferrous ion，and MDA levels，were less pronounced in HOS / DDP cells than in HOS cells．The SLC7A11 protein level showed no significant change． However，combined treatment with the ferroptosis inducer Erastin ( Era) resulted in significant decreases in viability，mitochondrial membrane potential，and the GSH / GSSG ratio in HOS / DDP cells ( P＜0. 05) ．Furthermore，the protein levels of p-STAT3，GPX4，and SLC7A11 were also markedly reduced ( P＜0. 05) ． Conclusion The activation of ferroptosis mediated by p-STAT3 enhances cisplatin sensitivity in HOS / DDP cells．

Objective To develop an automatic machine used for dispensing, filtering and filling the drug solution. Methods The microcomputer integrated with photoelectric -machinery technique is used to develop the automaitically opertating machines which have series of automatic functions,such as heating, stirring, dispensing, filtering and filling the drug solution, as well as digitally dispensing amount, heating temperature, filling bottle-number, and etc. Results The machine is suitable for automatically preparing,filtering a volume of 5,000ml to 30,000ml and automatically filling various specifications of drug solution with a volume of 30ml to ≥1,000ml in all kinds of army and locality hospital pharmacy. Conclusion This machine is characterized with novel and airtight design, and complete functions. It is suitable to be used not only in common but also under field condition of army.

Objective To develop an automatic machine used for rinsing and drying ampoules and filling drug succus in field condition. Methods By using the microcomputer integrated with photoelectric-machinery technique,a full-automatic and continuous production line are formed including such working procedures as delivery,inspection,rinsing,drying,perfusing,topping,sealing,counting on,etc. Results It can implement series of operations described above on a continuous and automatic production line for producing 5,10,20 and 30ml drug succus instead of tedious,manual operations and complicated devices. Conclusion This machine can save 75% of labor and equipment,and 90% of water-electric power and bottles,which is suitable not only for common use but also for field army use.

In this psper,the design principle,structure,distinguishing feature and composition of the Vacuum system Were outlined.The operating procedures of Vacuum suction filtration,distillation,con- centration and drying and the effects of comprehensive application were also introduced.