To enhance the practical ability and job competency of full-time master of public health (MPH) postgraduates and explore a collaborative training mode that integrates medical education with a prevention-and-control approach, in line with standardized public health physician training, the Beijing Center for Disease Control and Prevention, in collaboration with the School of Public Health affiliated to Capital Medical University, had recruited full-time MPH postgraduates since 2015. These students were trained and assessed through a collaborative training mode based on the Beijing public health physician training mechanism. Through the introduction and analysis of the training objectives, training process, practical assessment methods, training quality, and results, this article suggests that the construction of a collaborative training mode integrating MPH postgraduate education of public health professionals and standardized public health physician training has explored a new pathway for cultivating "four-certification integration" public health professionals. This aligns with the Chinese national strategy for public health talent development and can alleviate the problems of "contradictions between work and study", including the current shortage of public health physicians at present and the difficulties in standardized training enrollment. In addition, this collaborative training mode provides valuable experience for other medical schools in training applied public health professionals who meet national public health standards and combine prevention with treatment.

Obesity is a significant risk factor for cancer and is associated with breast cancer metastasis. Nevertheless, the mechanism by which alterations in systemic metabolism affect tumor microenvironment (TME) and consequently influence tumor metastasis remains inadequately understood. Herein, we found that perturbations in circulating metabolites induced by obesity promote metastasis-like phenotypes in breast cancer. Oleoylcarnitine (OLCarn) concentrations were elevated in the serum of obese mice and humans. Administration of exogenous OLCarn induces metastasis-like characteristics in breast cancer cells. Mechanistically, OLCarn directly interacts with the Arg176 site of adenylate cyclase 10 (ADCY10), leading to the activation of ADCY10 and enhancement of cAMP production. Mutations at Arg176 prevent OLCarn from binding to ADCY10, disrupting the ADCY10-mediated activation of cyclic adenosine monophosphate (cAMP) signaling pathway. This activation promotes transcription factor 4 (TCF4)-dependent kinesin family member C1 (KIFC1) transcription, thereby driving breast cancer metastasis. Conversely, the neutralization of both ADCY10 and KIFC1 through knockdown or pharmacological inhibition abrogates the oncogenic effects mediated by OLCarn. Hence, obesity-induced systemic environmental changes lead to the aberrant accumulation of OLCarn within the TME, making it a potential therapeutic target and biomarker for breast cancer.

OBJECTIVE T o establish the automatic diagnosis system for varicella pneumonia based on multi-slice spiral computed tomography(MSCT)and verify its application value.METHODS The clinical data were collected from 295 patients with varicella who were treated in the hospital from Jan.2016 to Mar.2023 and assigned as the training set,the pulmonary imaging findings were acquired from the MSCT chest scanning.The automatic diagno-sis system for varicella pneumonia was established based on MSCT with the use of convolutional neural network technology.Totally 279 patients with varicella who were treated during the same period were chosen as the valida-tion set,the result of comprehensive diagnosis was set as gold standard,and the efficiency of the above system in diagnosis of varicella pneumonia was observed.RESULTS Totally 279 patients with varicella were included in the validation group,243 of whom had varicella pneumonia,and 36 had simple varicella infection.The sensitivity of the automatic diagnosis system established based on MSCT was 97.53％in diagnosis of the varicella pneumonia of the validation group,with the specificity 91.67％,the accuracy 96.77％,respectively higher than 93.83％,83.33％and 92.47％of MSCT,and it was highly consistent with the gold standard(Kappa=0.919,P＜0.001);there was consistency between MSCT and the gold standards(Kappa=0.675,P＜0.001).CONCLUSION The au-tomatic diagnosis system for varicella pneumonia established based MSCT can raise the sensitivity,specificity and accuracy in diagnosis of varicella pneumonia and have the advantages of automation and convenience,and serve as a new tool for clinical diagnosis of varicella pneumonia.

To enhance the practical ability and job competency of full-time master of public health (MPH) postgraduates and explore a collaborative training mode that integrates medical education with a prevention-and-control approach, in line with standardized public health physician training, the Beijing Center for Disease Control and Prevention, in collaboration with the School of Public Health affiliated to Capital Medical University, had recruited full-time MPH postgraduates since 2015. These students were trained and assessed through a collaborative training mode based on the Beijing public health physician training mechanism. Through the introduction and analysis of the training objectives, training process, practical assessment methods, training quality, and results, this article suggests that the construction of a collaborative training mode integrating MPH postgraduate education of public health professionals and standardized public health physician training has explored a new pathway for cultivating "four-certification integration" public health professionals. This aligns with the Chinese national strategy for public health talent development and can alleviate the problems of "contradictions between work and study", including the current shortage of public health physicians at present and the difficulties in standardized training enrollment. In addition, this collaborative training mode provides valuable experience for other medical schools in training applied public health professionals who meet national public health standards and combine prevention with treatment.

OBJECTIVE T o establish the automatic diagnosis system for varicella pneumonia based on multi-slice spiral computed tomography(MSCT)and verify its application value.METHODS The clinical data were collected from 295 patients with varicella who were treated in the hospital from Jan.2016 to Mar.2023 and assigned as the training set,the pulmonary imaging findings were acquired from the MSCT chest scanning.The automatic diagno-sis system for varicella pneumonia was established based on MSCT with the use of convolutional neural network technology.Totally 279 patients with varicella who were treated during the same period were chosen as the valida-tion set,the result of comprehensive diagnosis was set as gold standard,and the efficiency of the above system in diagnosis of varicella pneumonia was observed.RESULTS Totally 279 patients with varicella were included in the validation group,243 of whom had varicella pneumonia,and 36 had simple varicella infection.The sensitivity of the automatic diagnosis system established based on MSCT was 97.53％in diagnosis of the varicella pneumonia of the validation group,with the specificity 91.67％,the accuracy 96.77％,respectively higher than 93.83％,83.33％and 92.47％of MSCT,and it was highly consistent with the gold standard(Kappa=0.919,P＜0.001);there was consistency between MSCT and the gold standards(Kappa=0.675,P＜0.001).CONCLUSION The au-tomatic diagnosis system for varicella pneumonia established based MSCT can raise the sensitivity,specificity and accuracy in diagnosis of varicella pneumonia and have the advantages of automation and convenience,and serve as a new tool for clinical diagnosis of varicella pneumonia.

AIM:This study aimed to observe the analgesic effects of Hegu acupoint catgut embedding in a rat model of labor and investigate its influence on biomarkers such as calcitonin gene-related peptide(CGRP)signals at the Hegu acupoint.METHODS:Thirty-six pregnant rats were randomly divided into three groups:control group,Hegu acu-puncture group,and Hegu catgut embedding group.Pain threshold changes were assessed using the tail immersion test and paw withdrawal thermal latency at four time points:pre-induction,before the onset of labor,at the onset of labor,and at the mid-stage of labor.Tissue samples from the Hegu acupoint were collected at the mid-stage of labor to detect the ex-pression of CGRP,substance P(SP),and mast cells using immunofluorescence.The concentrations of ATP and ade-nosine were measured using ELISA.RESULTS:Before labor induction,there was no significant difference in tail immer-sion test and paw withdrawal thermal latency among the three groups(P>0.05).Before the onset of labor,both the acu-puncture and catgut embedding groups exhibited significantly higher tail-flick times and paw withdrawal latencies com-pared to the control group(P<0.05).At labor initiation and mid-labor,the catgut embedding group had significantly higher tail-flick times and paw withdrawal latencies compared to both the control and acupuncture groups(P<0.05).During mid-labor,the expression of CGRP,SP,mast cells,ATP,and adenosine concentrations in the catgut embedding group was significantly higher than that in the control and acupuncture groups(P<0.05),with co-expression of CGRP,SP,and mast cells observed.CONCLUSION:Hegu acupoint catgut embedding effectively alleviates labor pain,and its mechanism may involve increased local expression of CGRP and SP,promoting mast cell degranulation,and increasing ATP release and its conversion to adenosine.

Objective To evaluate the diagnostic value of endobronchial ultrasound-transvascular needle aspiration(EBUS-TVNA)in hilum of lung and mediastinal lesions.Methods 10 cases of hilum of lung and mediastinal lesions who underwent EBUS-TVNA because vessels pass between the airway and lesions were selected from June 2021 to January 2023,the diagnostic rate and complications were retrospectively analyzed.Results Of the 10 patients,8 traversed the pulmonary artery or its branches,2 traversed aorta or superior vena cava.Specimens were successfully obtained from all patients and 8 of them got definitive diagnosis,including 6 malignancy and 2 benign lesions.The overall yield of EBUS-TVNA for hilum of lung and mediastinal lesions was 80.0%(8/10),the diagnostic sensitivity for malignancy was 85.7%(6/7),and the diagnostic specificity was 100.0%(3/3).The other 2 cases were not diagnosed,1 was diagnosed as bladder urothelial carcinoma by percutaneous lung puncture biopsy,and the other was classified as benign due to the reduced lesion after 6 months of follow-up.All of them had good tolerance to the operation,and no serious complications occurred.Conclusion EBUS-TVNA is a safe and effective technique for the diagnosis of hilum of lung and mediastinal lesions,it can be used as an effective auxiliary method to traditional ultrasound bronchoscopy techniques.

In recent years, the incidence of sleep disorders is increasing gradually, and sleep disorder has become a common and frequently-occurring disease in community. While drugs bring a series of side effects, non-pharmacological therapy has become a hotspot in sleep quality study field. It is found that"Gua sha" therapy has a significant effect in improving sleep quality as one of the traditional Chinese medicine (TCM) care techniques by literature review. This paper summarized the current situation and analyzed the deficiencies of "Gua sha" in sleep improvement basing on the theoretical foundation of Chinese medicine and biological mechanism, so as to provide references for future research and provide guidance for promoting the application of "Gua sha" therapy in community.

Objective To observe whether strength training of a healthy limb can produce a cross transport effect on the affected side.Methods Thirty hemiplegic stroke survivors were randomly divided into an experimental group and a control group,each of 15,using a random number table.Both groups received conventional rehabilitation,while the experimental group was given training to strengthen dorsiflexion and the isometric resistance of their healthy ankles for 6 weeks.The maximum voluntary contraction (MVC) and surface electromyography were measured before and after the training.The lower-extremity section of the simplified Fugl-Meyer motor function rating scale (FMA) was used to assess the subjects' motor function.Results After the training,the average FMA score of the experimental group was significantly better than before the treatment and also significantly better than that of the control group after the treatment.After the training,the average IEMGs of the tibialis anterior muscle and the gastrocnemius in the treatment group were significantly different from those of their ipsilateral side before the treatment and in the control group after treatment.Significant differences before and after the treatment were observed in the average MVC of both tibialis anterior and gastrocnemius muscles in the experimental group,and between the experimental group and control group averages.Conclusion Ankle strength training of hemiplegics' healthy limbs can strengthen the contralateral and antagonistic muscles,which indicates the cross transport of strength training.Isometric resistance training of the less-affected limb can improve the motor function of hemiplegics' lower extremities.

Objective:To explore the correlation between medication adherence and quality of life (QOL) in aged pa‐tients with hypertension from communities .Methods :Chinese questionnaire of quality of life in patients with cardi‐ovascular disease (CQQC) and Morisky medication adherence scale were used to investigate and analyze 197 aged pa‐tients with hypertension from communities .Results:Total score of CQQC was (78.69 ± 9.36) scores in the 197 aged patients with hypertension from communities .In which ,77 cases (39.09% ) possessed good medication adherence , and 120 cases ( (60. 91% )) with poor adherence to medication . Compared with those with poor adherence to medi‐cation,therewassignificantriseintotalscoreofCQQC [(75.73±8.52)scoresvs.(83.31±8.76)scores ,P<0.01] .Pearson correlation analysis indicated that QOL was significant positively correlated with medication adher‐ence (r=0.397 , P<0.01) .Conclusion:Community health staff should pay attention to influencing factors of med‐ication adherence in aged patients with hypertension ,take individualized and targeted interventional measures to im‐prove their adherence to medicationand quality of life .