Objective : To explore the effect of Wilms′ tumor 1-associated protein(WTAP) on tissue collagen deposition in pulmonary fibrosis caused by bleomycin. Methods : 60 mice were randomly divided into four groups: control group(Control group), Bleomycin-induced pulmonary fibrosis group(BLM group), pulmonary fibrosis lentivirus empty vector control group(BLM+LV-NC group), pulmonary fibrosis WTAP lentivirus group virus group(BLM+LV-WTAP group). Experimental pulmonary fibrosis mouse model was established by subcutaneous injection of bleomycin(35 mg/kg) into the abdomen, twice a week for a total of 8 times. After modeling, Western Blot was used to detect the protein expression of fibrosis-related markers α-smooth muscle actin(α-SMA), type I collagen(Collagen Ⅰ), fibronectin(Fibronectin), and WTAP protein. Masson staining and Sirius Red staining were used to detect collagen deposition. RT-qPCR was used to detect WTAP mRNA expression, WTAP lentivirus infection effect, and Collagen Ⅰ mRNA expression. Results: Compared with the Control group, the expression of pulmonary fibrosis markers α-SMA(P<0.001), Collagen Ⅰ(P<0.001), and Fibronectin(P<0.01) protein in the BLM group all increased. Masson staining(P<0.001) and Sirius Red staining(P<0.001) confirmed that significant collagen deposition occurred in the lung tissue of the BLM group. In addition, the expression of WTAP protein in the lung tissue of the BLM group increased(P<0.01). Compared with the Control group, the expression of WTAP mRNA in the BLM group increased(P<0.001). Compared with the BLM+LV-NC group, the expression of WTAP mRNA in the tissues of the BLM+LV-WTAP group decreased(P<0.001), proving that virus infection is effective. After infection with WTAP lentivirus, collagen fiber deposition decreased(P<0.001), Collagen Ⅰ mRNA(P<0.001) level decreased, and protein(P<0.001) expression decreased in the BLM+LV-WTAP group. Conclusion Knocking down of WTAP can reduce collagen deposition in bleomycin-induced pulmonary fibrosis tissue in mice and improve experimental pulmonary fibrosis.

Objective: To investigate the effect of sirtuin 2(SIRT2) on the proliferation and migration of cardiac fibroblasts(CFs)in C57BL/6 mice under angiotensin II(Ang Ⅱ) stimulation. Methods : The hearts were taken from 1 to 2 days C57BL/6 milk mice. After cutting and digesting, CFs were extracted by different adhesion centrifugation. After CFs attachment, the cells were cultured under control medium and Ang Ⅱ(100 nmol/L) medium and treated using OE-SIRT2 plasmid to overexpression the SIRT2 gene. RT-qPCR was used to detect mRNA expression of SIRT2 proliferating cell nuclear antigen(PCNA), periostin(POSTN)and type Ⅰ collagen procollagen A1(Col1A1), Western blot assay was used to measure the protein expression levels of SIRT2, PCNA, POSTN and Col1A1, CCK-8 assay and EdU assay were used to evaluate CFs proliferation rate, Transwell experiment was used to assess CFs migration activity. Results: Compared with control group, Ang Ⅱ stimulation led to down-regulation of SIRT2 expression in CFs, increased collagen expression, and promoted CFs proliferation and migration. The expression of SIRT2 was up regulated in CFs treated with OE-SIRT2 plasmid under Ang Ⅱ stimulation, Col1A1, POSTN and PCNA expression was down regulated, and CFs proliferation and migration ability decreased. Conclusion Overexpression of SIRT2 can inhibit the proliferation and migration of CFs under Ang Ⅱ stimulation, indicating that SIRT2 may be a key regulatory point in the onset and progression of cardiac fibrosis.

Objective To explore the application of change speed of lithotomy position in the patients with gynecological laparoscopic surgery.Methods A total of 120 gynecological laparoscopic surgery patients in our hospital were randomly divided into observation group and control group,pneumoperitoneum pressure of two groups was 11 mmHg.The change speed of lithotomy position in the control group was in 6 s,the observation group was ＞ 30 s,and the changes of hemodynamics and blood gas indexes were compared.Results SBP and DBP after pneumoperitoneum in the two groups were increased compared with that before pneumoperitoneum (P ＜ 0.05).The increase of DBP and SBP after pneumoperitoneum in the observation group was less than that in the control group (P ＜ 0.05).PETCO2 after pneumoperitoneum in the observation group showed no statistically significant compared with pneumoperitoneum before (P ＞ 0.05),but the control group had higher PETCO2 than that before pneumoperitoneum,there were statistically significant differences (P ＜ 0.05).The observation group and the control group had a tendency to rise in airway pressure after pneumoperitoneum,but no statistical significance between the two groups was found (P ＞ 0.05).Conclusion Prolonging change time of lithotomy position can reduce the influence of hemodynamics for the patients with gynecological laparoscopic operation,and it is beneficial to the recovery of lung function.

Objective To explore the application of change speed of lithotomy position in the patients with gynecological laparoscopic surgery.Methods A total of 120 gynecological laparoscopic surgery patients in our hospital were randomly divided into observation group and control group,pneumoperitoneum pressure of two groups was 11 mmHg.The change speed of lithotomy position in the control group was in 6 s,the observation group was ＞ 30 s,and the changes of hemodynamics and blood gas indexes were compared.Results SBP and DBP after pneumoperitoneum in the two groups were increased compared with that before pneumoperitoneum (P ＜ 0.05).The increase of DBP and SBP after pneumoperitoneum in the observation group was less than that in the control group (P ＜ 0.05).PETCO2 after pneumoperitoneum in the observation group showed no statistically significant compared with pneumoperitoneum before (P ＞ 0.05),but the control group had higher PETCO2 than that before pneumoperitoneum,there were statistically significant differences (P ＜ 0.05).The observation group and the control group had a tendency to rise in airway pressure after pneumoperitoneum,but no statistical significance between the two groups was found (P ＞ 0.05).Conclusion Prolonging change time of lithotomy position can reduce the influence of hemodynamics for the patients with gynecological laparoscopic operation,and it is beneficial to the recovery of lung function.